Anabarilius grahami (Kanglang fish) (Barilius grahami)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Osteoglossocephalai; Clupeocephala; Otomorpha; Ostariophysi; Otophysi;

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 23663 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3N0XLN5|A0A3N0XLN5_ANAGA Uncharacterized protein OS=Anabarilius grahami OX=495550 GN=DPX16_2126 PE=4 SV=1
MM1 pKa = 7.87DD2 pKa = 4.94RR3 pKa = 11.84NIYY6 pKa = 9.1MLIRR10 pKa = 11.84HH11 pKa = 6.42RR12 pKa = 11.84NGQSTLHH19 pKa = 5.61ATIIDD24 pKa = 3.52GDD26 pKa = 3.82VRR28 pKa = 11.84RR29 pKa = 11.84EE30 pKa = 3.77IPEE33 pKa = 3.8IAVPSHH39 pKa = 7.38DD40 pKa = 3.06EE41 pKa = 3.83VLFNRR46 pKa = 11.84VVQAVCRR53 pKa = 11.84LTRR56 pKa = 11.84YY57 pKa = 9.21PLTPAPDD64 pKa = 3.29RR65 pKa = 11.84QYY67 pKa = 11.89NPLLSMLAWNADD79 pKa = 2.98AWGVSTMTATGDD91 pKa = 3.35EE92 pKa = 4.34SDD94 pKa = 3.09IAFYY98 pKa = 10.0PVYY101 pKa = 10.5NNPPTEE107 pKa = 3.76RR108 pKa = 11.84VEE110 pKa = 4.02RR111 pKa = 11.84FRR113 pKa = 11.84IEE115 pKa = 3.94HH116 pKa = 6.75RR117 pKa = 11.84SVQPRR122 pKa = 11.84PSGEE126 pKa = 3.93SHH128 pKa = 4.69MHH130 pKa = 6.78GSRR133 pKa = 11.84TDD135 pKa = 3.58DD136 pKa = 3.33EE137 pKa = 4.93HH138 pKa = 7.32PEE140 pKa = 4.02PSHH143 pKa = 6.97LDD145 pKa = 3.44NNSEE149 pKa = 3.92IGSDD153 pKa = 3.69FDD155 pKa = 4.97RR156 pKa = 11.84SSEE159 pKa = 4.21SLGSSLIGEE168 pKa = 4.97ASTDD172 pKa = 3.71DD173 pKa = 4.1DD174 pKa = 4.41QSGSSQMDD182 pKa = 3.53NNSDD186 pKa = 3.06SGYY189 pKa = 10.33YY190 pKa = 10.75SNDD193 pKa = 3.05SSDD196 pKa = 3.86SLSSSFNPQASLGEE210 pKa = 3.96TSRR213 pKa = 11.84DD214 pKa = 3.6DD215 pKa = 3.85EE216 pKa = 4.62QPGPSHH222 pKa = 6.93LVNNSKK228 pKa = 9.83IRR230 pKa = 11.84SNSDD234 pKa = 2.97CSINSLSSSCFPPASLRR251 pKa = 11.84EE252 pKa = 3.97TSTDD256 pKa = 3.52DD257 pKa = 3.84DD258 pKa = 5.08LSGSSHH264 pKa = 6.53MDD266 pKa = 3.1NNSEE270 pKa = 4.04IVFDD274 pKa = 4.48SDD276 pKa = 3.79CSSDD280 pKa = 3.22SFYY283 pKa = 11.64SLASLGEE290 pKa = 3.81ISTDD294 pKa = 3.6DD295 pKa = 4.08DD296 pKa = 4.87LSGSSHH302 pKa = 5.41MVNNSEE308 pKa = 4.59IGFDD312 pKa = 3.94SDD314 pKa = 3.93CSSDD318 pKa = 3.22SFYY321 pKa = 11.64SLASLGEE328 pKa = 3.81ISTDD332 pKa = 3.68DD333 pKa = 4.25DD334 pKa = 4.99LSGSPHH340 pKa = 5.64MVNNSEE346 pKa = 4.17IGFDD350 pKa = 3.94SDD352 pKa = 3.93CSSDD356 pKa = 3.22SFYY359 pKa = 11.64SLASLGEE366 pKa = 3.81ISTDD370 pKa = 3.68DD371 pKa = 4.25DD372 pKa = 4.99LSGSPHH378 pKa = 5.64MVNNSEE384 pKa = 4.17IGFDD388 pKa = 3.94SDD390 pKa = 3.93CSSDD394 pKa = 3.22SFYY397 pKa = 11.64SLASLGEE404 pKa = 3.81ISTDD408 pKa = 3.68DD409 pKa = 4.25DD410 pKa = 4.99LSGSPHH416 pKa = 5.64MVNNSEE422 pKa = 4.17IGFDD426 pKa = 3.94SDD428 pKa = 3.93CSSDD432 pKa = 3.22SFYY435 pKa = 11.64SLASLGEE442 pKa = 3.81ISTDD446 pKa = 3.68DD447 pKa = 4.25DD448 pKa = 4.99LSGSPHH454 pKa = 5.64MVNNSEE460 pKa = 4.17IGFDD464 pKa = 3.94SDD466 pKa = 3.93CSSDD470 pKa = 3.22SFYY473 pKa = 11.64SLASLGEE480 pKa = 3.81ISTDD484 pKa = 3.68DD485 pKa = 4.25DD486 pKa = 4.99LSGSPHH492 pKa = 5.64MVNNSEE498 pKa = 4.17IGFDD502 pKa = 3.94SDD504 pKa = 3.93CSSDD508 pKa = 3.22SFYY511 pKa = 11.64SLASLGEE518 pKa = 3.81ISTDD522 pKa = 3.68DD523 pKa = 4.25DD524 pKa = 4.99LSGSPHH530 pKa = 5.64MVNNSEE536 pKa = 4.17IGFDD540 pKa = 3.79SDD542 pKa = 4.03CTLDD546 pKa = 3.94LSS548 pKa = 4.34

Molecular weight: 58.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3N0XMM2|A0A3N0XMM2_ANAGA Tyrosine-protein phosphatase non-receptor type 21 OS=Anabarilius grahami OX=495550 GN=DPX16_2139 PE=3 SV=1
MM1 pKa = 7.01VVQVAQSTMVVQVAQGTMAVQVAQGTMAMQVAQGTMVVQVAQGTMAVQVAQGTMAMQVAQGTMVVQLAQGPMVVQVAQRR80 pKa = 11.84PMAMQVAQGTMVVQLAQGPMVVQVAQRR107 pKa = 11.84PMVVQVAHH115 pKa = 7.08RR116 pKa = 11.84PMAVQVAHH124 pKa = 7.29RR125 pKa = 11.84PMAMQVAQGTMVVQLAQGPMVVQVAQRR152 pKa = 11.84PMAMQVAQGTMVVQLAQGPMVVQVAQRR179 pKa = 11.84PMVVQVAHH187 pKa = 6.96RR188 pKa = 11.84PMVVQVAQGTMVMQVAQGAMVMQVAQGAMVVV219 pKa = 3.42

Molecular weight: 22.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

23663

0

23663

11186444

49

34580

472.7

52.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.37 ± 0.016

2.315 ± 0.013

5.182 ± 0.012

6.988 ± 0.029

3.624 ± 0.013

5.938 ± 0.022

2.69 ± 0.01

4.554 ± 0.014

5.743 ± 0.026

9.432 ± 0.023

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.379 ± 0.009

3.895 ± 0.012

5.598 ± 0.022

4.676 ± 0.019

5.754 ± 0.016

8.87 ± 0.026

5.787 ± 0.018

6.361 ± 0.019

1.172 ± 0.007

2.619 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski