Medicago sativa amalgavirus 1
Average proteome isoelectric point is 6.42
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2V0A0B8|A0A2V0A0B8_9VIRU ORF1+2p OS=Medicago sativa amalgavirus 1 OX=2069326 GN=ORF1+ORF2 PE=4 SV=1
MM1 pKa = 7.95 ADD3 pKa = 4.38 FEE5 pKa = 4.39 AQRR8 pKa = 11.84 DD9 pKa = 3.92 DD10 pKa = 5.06 NIAANAPIGGLSSKK24 pKa = 8.01 QQEE27 pKa = 4.29 IDD29 pKa = 3.4 TVTAAITPLLAAGFPQAIFNYY50 pKa = 10.32 DD51 pKa = 3.5 DD52 pKa = 4.24 LLLRR56 pKa = 11.84 GYY58 pKa = 7.12 TAKK61 pKa = 10.03 TFCDD65 pKa = 5.23 FIKK68 pKa = 10.16 PLSAITEE75 pKa = 4.04 RR76 pKa = 11.84 RR77 pKa = 11.84 EE78 pKa = 4.35 LISLCALGNNRR89 pKa = 11.84 FWDD92 pKa = 3.6 MSVVAEE98 pKa = 4.57 LDD100 pKa = 3.35 EE101 pKa = 4.34 FLNFVKK107 pKa = 10.02 WLKK110 pKa = 10.79 SPEE113 pKa = 3.9 GRR115 pKa = 11.84 DD116 pKa = 3.51 AQTQAAKK123 pKa = 10.32 KK124 pKa = 9.67 RR125 pKa = 11.84 ALNKK129 pKa = 9.82 KK130 pKa = 9.92 ASDD133 pKa = 3.5 GMSTKK138 pKa = 10.57 DD139 pKa = 3.32 VALVQMGNAIIADD152 pKa = 3.74 YY153 pKa = 10.58 QRR155 pKa = 11.84 EE156 pKa = 4.18 RR157 pKa = 11.84 KK158 pKa = 8.4 QRR160 pKa = 11.84 RR161 pKa = 11.84 FPIEE165 pKa = 3.64 EE166 pKa = 3.69 EE167 pKa = 3.84 MAEE170 pKa = 4.25 LRR172 pKa = 11.84 RR173 pKa = 11.84 QLRR176 pKa = 11.84 QLDD179 pKa = 4.0 EE180 pKa = 4.07 EE181 pKa = 4.34 LQAVEE186 pKa = 4.6 EE187 pKa = 4.45 EE188 pKa = 4.43 IKK190 pKa = 11.01 VKK192 pKa = 10.49 YY193 pKa = 10.57 GPVALYY199 pKa = 8.86 EE200 pKa = 4.64 GPDD203 pKa = 3.4 NTRR206 pKa = 11.84 VKK208 pKa = 10.78 SDD210 pKa = 3.59 AYY212 pKa = 11.43 LMYY215 pKa = 10.52 QDD217 pKa = 4.71 DD218 pKa = 4.42 CRR220 pKa = 11.84 KK221 pKa = 9.74 KK222 pKa = 9.78 GYY224 pKa = 9.7 RR225 pKa = 11.84 AIAQYY230 pKa = 10.71 QGGFEE235 pKa = 4.14 KK236 pKa = 10.62 AVEE239 pKa = 3.94 LFGNKK244 pKa = 7.29 VRR246 pKa = 11.84 EE247 pKa = 4.21 AHH249 pKa = 5.71 FCAYY253 pKa = 10.48 LSDD256 pKa = 4.01 PARR259 pKa = 11.84 SDD261 pKa = 3.67 FVRR264 pKa = 11.84 DD265 pKa = 3.53 YY266 pKa = 9.79 YY267 pKa = 10.07 NQKK270 pKa = 9.46 IVHH273 pKa = 6.41 LEE275 pKa = 3.55 RR276 pKa = 11.84 SGEE279 pKa = 4.08 KK280 pKa = 9.92 KK281 pKa = 9.73 QAGSFRR287 pKa = 11.84 SLLVAAHH294 pKa = 6.63 GEE296 pKa = 4.24 VAVNVPIVNPFNFDD310 pKa = 3.56 GEE312 pKa = 4.4 DD313 pKa = 3.37 SGRR316 pKa = 11.84 EE317 pKa = 3.86 SSQTGGNANQPPATKK332 pKa = 10.0 RR333 pKa = 11.84 RR334 pKa = 11.84 RR335 pKa = 11.84 QKK337 pKa = 10.73 SRR339 pKa = 11.84 AEE341 pKa = 3.92 RR342 pKa = 11.84 EE343 pKa = 4.2 AEE345 pKa = 3.9 RR346 pKa = 11.84 LQIDD350 pKa = 3.77 QPVRR354 pKa = 11.84 TQARR358 pKa = 11.84 TSRR361 pKa = 11.84 VHH363 pKa = 5.9 NKK365 pKa = 9.3 SRR367 pKa = 11.84 NAGGQDD373 pKa = 2.87 QDD375 pKa = 3.82 LQIVGVGAGEE385 pKa = 4.81 DD386 pKa = 3.72 DD387 pKa = 3.76 PHH389 pKa = 7.84 VQEE392 pKa = 4.79 QVV394 pKa = 2.81
Molecular weight: 44.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.002
IPC2_protein 6.02
IPC_protein 6.084
Toseland 6.313
ProMoST 6.389
Dawson 6.313
Bjellqvist 6.3
Wikipedia 6.3
Rodwell 6.287
Grimsley 6.415
Solomon 6.313
Lehninger 6.3
Nozaki 6.547
DTASelect 6.722
Thurlkill 6.722
EMBOSS 6.693
Sillero 6.664
Patrickios 4.571
IPC_peptide 6.326
IPC2_peptide 6.532
IPC2.peptide.svr19 6.616
Protein with the highest isoelectric point:
>tr|A0A2V0A0B8|A0A2V0A0B8_9VIRU ORF1+2p OS=Medicago sativa amalgavirus 1 OX=2069326 GN=ORF1+ORF2 PE=4 SV=1
MM1 pKa = 7.95 ADD3 pKa = 4.38 FEE5 pKa = 4.39 AQRR8 pKa = 11.84 DD9 pKa = 3.92 DD10 pKa = 5.06 NIAANAPIGGLSSKK24 pKa = 8.01 QQEE27 pKa = 4.29 IDD29 pKa = 3.4 TVTAAITPLLAAGFPQAIFNYY50 pKa = 10.32 DD51 pKa = 3.5 DD52 pKa = 4.24 LLLRR56 pKa = 11.84 GYY58 pKa = 7.12 TAKK61 pKa = 10.03 TFCDD65 pKa = 5.23 FIKK68 pKa = 10.16 PLSAITEE75 pKa = 4.04 RR76 pKa = 11.84 RR77 pKa = 11.84 EE78 pKa = 4.35 LISLCALGNNRR89 pKa = 11.84 FWDD92 pKa = 3.6 MSVVAEE98 pKa = 4.57 LDD100 pKa = 3.35 EE101 pKa = 4.34 FLNFVKK107 pKa = 10.02 WLKK110 pKa = 10.79 SPEE113 pKa = 3.9 GRR115 pKa = 11.84 DD116 pKa = 3.51 AQTQAAKK123 pKa = 10.32 KK124 pKa = 9.67 RR125 pKa = 11.84 ALNKK129 pKa = 9.82 KK130 pKa = 9.92 ASDD133 pKa = 3.5 GMSTKK138 pKa = 10.57 DD139 pKa = 3.32 VALVQMGNAIIADD152 pKa = 3.74 YY153 pKa = 10.58 QRR155 pKa = 11.84 EE156 pKa = 4.18 RR157 pKa = 11.84 KK158 pKa = 8.4 QRR160 pKa = 11.84 RR161 pKa = 11.84 FPIEE165 pKa = 3.64 EE166 pKa = 3.69 EE167 pKa = 3.84 MAEE170 pKa = 4.25 LRR172 pKa = 11.84 RR173 pKa = 11.84 QLRR176 pKa = 11.84 QLDD179 pKa = 4.0 EE180 pKa = 4.07 EE181 pKa = 4.34 LQAVEE186 pKa = 4.6 EE187 pKa = 4.45 EE188 pKa = 4.43 IKK190 pKa = 11.01 VKK192 pKa = 10.49 YY193 pKa = 10.57 GPVALYY199 pKa = 8.86 EE200 pKa = 4.64 GPDD203 pKa = 3.4 NTRR206 pKa = 11.84 VKK208 pKa = 10.78 SDD210 pKa = 3.59 AYY212 pKa = 11.43 LMYY215 pKa = 10.52 QDD217 pKa = 4.71 DD218 pKa = 4.42 CRR220 pKa = 11.84 KK221 pKa = 9.74 KK222 pKa = 9.78 GYY224 pKa = 9.7 RR225 pKa = 11.84 AIAQYY230 pKa = 10.71 QGGFEE235 pKa = 4.14 KK236 pKa = 10.62 AVEE239 pKa = 3.94 LFGNKK244 pKa = 7.29 VRR246 pKa = 11.84 EE247 pKa = 4.21 AHH249 pKa = 5.71 FCAYY253 pKa = 10.48 LSDD256 pKa = 4.01 PARR259 pKa = 11.84 SDD261 pKa = 3.67 FVRR264 pKa = 11.84 DD265 pKa = 3.53 YY266 pKa = 9.79 YY267 pKa = 10.07 NQKK270 pKa = 9.46 IVHH273 pKa = 6.41 LEE275 pKa = 3.55 RR276 pKa = 11.84 SGEE279 pKa = 4.08 KK280 pKa = 9.98 KK281 pKa = 9.91 QAGSFAVSWWPLMEE295 pKa = 4.54 KK296 pKa = 9.44 WLLMFPLSTRR306 pKa = 11.84 STLMEE311 pKa = 5.3 KK312 pKa = 10.09 IPVGKK317 pKa = 8.72 VHH319 pKa = 7.07 KK320 pKa = 10.31 PGEE323 pKa = 4.46 TPTNRR328 pKa = 11.84 PLLRR332 pKa = 11.84 GADD335 pKa = 3.42 KK336 pKa = 11.25 SRR338 pKa = 11.84 VLNVRR343 pKa = 11.84 QSGYY347 pKa = 10.79 KK348 pKa = 10.01 SISRR352 pKa = 11.84 LEE354 pKa = 4.25 RR355 pKa = 11.84 KK356 pKa = 9.69 HH357 pKa = 6.23 EE358 pKa = 3.98 LQGCITSQGMPEE370 pKa = 3.72 GRR372 pKa = 11.84 IKK374 pKa = 10.4 IFKK377 pKa = 9.66 SWEE380 pKa = 4.05 SEE382 pKa = 4.0 PGRR385 pKa = 11.84 MIPMSRR391 pKa = 11.84 SKK393 pKa = 11.12 YY394 pKa = 7.64 EE395 pKa = 3.86 CAVRR399 pKa = 11.84 WVIGGGEE406 pKa = 3.96 VRR408 pKa = 11.84 NWKK411 pKa = 9.66 VDD413 pKa = 2.82 SSMYY417 pKa = 10.35 RR418 pKa = 11.84 GGGSSNDD425 pKa = 3.5 ALKK428 pKa = 11.08 LLANASTVRR437 pKa = 11.84 PGKK440 pKa = 10.0 LLRR443 pKa = 11.84 DD444 pKa = 3.67 VYY446 pKa = 10.47 SFRR449 pKa = 11.84 VARR452 pKa = 11.84 ARR454 pKa = 11.84 LQLPGDD460 pKa = 4.01 FSVPDD465 pKa = 4.08 GPDD468 pKa = 2.69 ACRR471 pKa = 11.84 VKK473 pKa = 10.94 NFNNFATSGPVLKK486 pKa = 10.94 AFGVRR491 pKa = 11.84 SKK493 pKa = 11.42 NGLRR497 pKa = 11.84 QLLQDD502 pKa = 3.67 EE503 pKa = 4.49 AWWYY507 pKa = 10.52 FNSFGNSDD515 pKa = 3.93 FGVEE519 pKa = 3.94 GLPWFGARR527 pKa = 11.84 LGFRR531 pKa = 11.84 SKK533 pKa = 10.97 LVTEE537 pKa = 4.58 EE538 pKa = 3.97 KK539 pKa = 10.41 ARR541 pKa = 11.84 KK542 pKa = 9.2 KK543 pKa = 10.28 ISEE546 pKa = 4.15 GDD548 pKa = 3.75 SVGRR552 pKa = 11.84 AVMMMDD558 pKa = 5.17 ALEE561 pKa = 4.23 QCCSSPLYY569 pKa = 10.51 NVLSTYY575 pKa = 8.45 TFHH578 pKa = 7.56 KK579 pKa = 10.07 RR580 pKa = 11.84 LNRR583 pKa = 11.84 RR584 pKa = 11.84 SGFKK588 pKa = 10.34 NAVVKK593 pKa = 10.89 ASSDD597 pKa = 3.05 WAHH600 pKa = 6.24 IWNGVKK606 pKa = 10.23 DD607 pKa = 3.51 AAVIVEE613 pKa = 4.4 LDD615 pKa = 2.89 WSKK618 pKa = 10.98 FDD620 pKa = 3.95 RR621 pKa = 11.84 EE622 pKa = 4.19 RR623 pKa = 11.84 PRR625 pKa = 11.84 EE626 pKa = 3.97 DD627 pKa = 3.66 LEE629 pKa = 4.65 FMVSLISSCFNPKK642 pKa = 9.27 SARR645 pKa = 11.84 EE646 pKa = 3.8 EE647 pKa = 4.22 RR648 pKa = 11.84 LLHH651 pKa = 7.2 AYY653 pKa = 5.92 TTSNFRR659 pKa = 11.84 ALVEE663 pKa = 4.16 RR664 pKa = 11.84 PVFLDD669 pKa = 3.17 GGGVFGIEE677 pKa = 3.97 GMVPSGSLWTGWLDD691 pKa = 3.31 TALNILYY698 pKa = 8.53 MKK700 pKa = 10.05 AVCAEE705 pKa = 4.06 IGVGDD710 pKa = 4.56 DD711 pKa = 4.32 DD712 pKa = 5.55 VEE714 pKa = 4.64 VMCAGDD720 pKa = 4.8 DD721 pKa = 3.67 NLTLFKK727 pKa = 10.59 FDD729 pKa = 4.01 PGEE732 pKa = 4.06 ANLRR736 pKa = 11.84 RR737 pKa = 11.84 IRR739 pKa = 11.84 DD740 pKa = 3.74 LLNDD744 pKa = 3.7 WFLAGISEE752 pKa = 4.74 DD753 pKa = 3.12 EE754 pKa = 4.4 FLIHH758 pKa = 6.97 RR759 pKa = 11.84 PPYY762 pKa = 10.28 HH763 pKa = 4.66 VTKK766 pKa = 10.7 VQACFPEE773 pKa = 4.8 GTDD776 pKa = 3.77 LSRR779 pKa = 11.84 GTSGMLKK786 pKa = 9.64 KK787 pKa = 10.59 AVWVPFEE794 pKa = 4.32 GEE796 pKa = 4.29 VVIDD800 pKa = 3.58 NARR803 pKa = 11.84 GRR805 pKa = 11.84 SHH807 pKa = 6.44 RR808 pKa = 11.84 WEE810 pKa = 4.06 YY811 pKa = 10.99 RR812 pKa = 11.84 FSGCPKK818 pKa = 10.15 FLSCYY823 pKa = 8.11 WLHH826 pKa = 7.32 DD827 pKa = 3.8 GLPIRR832 pKa = 11.84 PAHH835 pKa = 6.99 DD836 pKa = 3.77 NLEE839 pKa = 4.15 KK840 pKa = 10.97 LLWPEE845 pKa = 4.36 GLHH848 pKa = 7.51 DD849 pKa = 6.67 DD850 pKa = 3.4 IDD852 pKa = 4.38 VYY854 pKa = 10.95 EE855 pKa = 4.41 GAVISMVVDD864 pKa = 3.69 NPHH867 pKa = 5.86 NHH869 pKa = 6.37 HH870 pKa = 6.75 NCNHH874 pKa = 5.61 MLSRR878 pKa = 11.84 FIIIRR883 pKa = 11.84 EE884 pKa = 3.93 ARR886 pKa = 11.84 RR887 pKa = 11.84 LGASVEE893 pKa = 4.39 DD894 pKa = 3.7 PFIPIKK900 pKa = 10.37 HH901 pKa = 5.49 GSIRR905 pKa = 11.84 PVGDD909 pKa = 3.26 EE910 pKa = 3.72 PVPYY914 pKa = 10.02 PEE916 pKa = 4.13 LAPWRR921 pKa = 11.84 RR922 pKa = 11.84 APGGYY927 pKa = 9.84 KK928 pKa = 10.4 LEE930 pKa = 5.09 DD931 pKa = 3.48 YY932 pKa = 10.68 PEE934 pKa = 4.29 NAEE937 pKa = 4.42 HH938 pKa = 6.15 IQVFRR943 pKa = 11.84 DD944 pKa = 3.97 FMQGVSSLYY953 pKa = 10.32 LRR955 pKa = 11.84 EE956 pKa = 4.05 ATGGVDD962 pKa = 2.83 AWQFMDD968 pKa = 5.25 IIRR971 pKa = 11.84 GDD973 pKa = 3.53 AFVGEE978 pKa = 4.47 GQFGNDD984 pKa = 3.17 LRR986 pKa = 11.84 SWLNWLHH993 pKa = 5.94 HH994 pKa = 6.34 HH995 pKa = 7.41 PISKK999 pKa = 10.09 YY1000 pKa = 10.17 LRR1002 pKa = 11.84 EE1003 pKa = 4.18 TKK1005 pKa = 9.23 TFRR1008 pKa = 11.84 LKK1010 pKa = 10.98 DD1011 pKa = 3.52 GADD1014 pKa = 3.3 PDD1016 pKa = 4.0 APKK1019 pKa = 10.72 DD1020 pKa = 3.63 LEE1022 pKa = 4.2 KK1023 pKa = 10.86 AGRR1026 pKa = 11.84 ALRR1029 pKa = 11.84 ALRR1032 pKa = 11.84 GRR1034 pKa = 11.84 LEE1036 pKa = 4.34 LGGFACVEE1044 pKa = 4.13 DD1045 pKa = 4.49 FVRR1048 pKa = 11.84 WLDD1051 pKa = 4.05 GIRR1054 pKa = 11.84 SSRR1057 pKa = 11.84 EE1058 pKa = 3.58
Molecular weight: 120.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.832
IPC2_protein 7.22
IPC_protein 7.088
Toseland 6.898
ProMoST 7.629
Dawson 7.775
Bjellqvist 8.126
Wikipedia 7.688
Rodwell 7.79
Grimsley 6.985
Solomon 7.805
Lehninger 7.819
Nozaki 8.361
DTASelect 7.936
Thurlkill 7.98
EMBOSS 8.009
Sillero 8.273
Patrickios 4.571
IPC_peptide 7.805
IPC2_peptide 7.249
IPC2.peptide.svr19 7.225
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1452
394
1058
726.0
82.16
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.609 ± 1.223
1.446 ± 0.229
6.749 ± 0.19
7.163 ± 0.374
4.683 ± 0.33
7.576 ± 0.518
1.86 ± 0.313
4.339 ± 0.013
5.854 ± 0.126
8.54 ± 0.76
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.135 ± 0.325
4.063 ± 0.268
4.545 ± 0.392
3.926 ± 1.687
8.196 ± 0.096
6.129 ± 0.559
2.961 ± 0.179
6.405 ± 0.032
1.928 ± 0.754
2.893 ± 0.081
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here