Streptococcus phage Javan11
Average proteome isoelectric point is 6.33
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZYM0|A0A4D5ZYM0_9CAUD Uncharacterized protein OS=Streptococcus phage Javan11 OX=2547980 GN=Javan11_0020 PE=4 SV=1
MM1 pKa = 8.06 DD2 pKa = 5.52 RR3 pKa = 11.84 GLFGTFDD10 pKa = 3.81 YY11 pKa = 11.27 DD12 pKa = 4.15 RR13 pKa = 11.84 DD14 pKa = 3.85 NLQPEE19 pKa = 4.54 PEE21 pKa = 4.13 RR22 pKa = 11.84 EE23 pKa = 3.91 EE24 pKa = 4.35 RR25 pKa = 11.84 DD26 pKa = 3.18 PDD28 pKa = 2.91 EE29 pKa = 3.78 WLFRR33 pKa = 11.84 GGQWIYY39 pKa = 11.19 VGDD42 pKa = 3.89 YY43 pKa = 10.77
Molecular weight: 5.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.279
IPC2_protein 4.062
IPC_protein 3.948
Toseland 3.745
ProMoST 4.113
Dawson 3.935
Bjellqvist 4.101
Wikipedia 3.884
Rodwell 3.783
Grimsley 3.668
Solomon 3.923
Lehninger 3.872
Nozaki 4.062
DTASelect 4.266
Thurlkill 3.808
EMBOSS 3.884
Sillero 4.062
Patrickios 3.681
IPC_peptide 3.91
IPC2_peptide 4.037
IPC2.peptide.svr19 3.994
Protein with the highest isoelectric point:
>tr|A0A4D5ZXS9|A0A4D5ZXS9_9CAUD Membrane protein OS=Streptococcus phage Javan11 OX=2547980 GN=Javan11_0047 PE=4 SV=1
MM1 pKa = 6.62 GTSVSVKK8 pKa = 9.68 IDD10 pKa = 3.29 LKK12 pKa = 11.05 GIEE15 pKa = 4.56 KK16 pKa = 10.23 KK17 pKa = 10.93 VSSTALAKK25 pKa = 10.58 GKK27 pKa = 9.91 LAIASQMKK35 pKa = 9.42 MDD37 pKa = 4.08 STPFIPRR44 pKa = 11.84 KK45 pKa = 9.94 NGEE48 pKa = 4.2 LSGSAQVSKK57 pKa = 11.26 DD58 pKa = 3.22 GIRR61 pKa = 11.84 YY62 pKa = 6.35 PGPYY66 pKa = 10.0 ARR68 pKa = 11.84 AQFYY72 pKa = 10.01 GSSYY76 pKa = 10.07 NKK78 pKa = 9.58 HH79 pKa = 6.23 RR80 pKa = 11.84 SFTFKK85 pKa = 10.68 RR86 pKa = 11.84 YY87 pKa = 5.03 TTPGTGKK94 pKa = 10.27 RR95 pKa = 11.84 WDD97 pKa = 3.89 LKK99 pKa = 11.18 ASALHH104 pKa = 5.99 IKK106 pKa = 10.19 DD107 pKa = 2.95 WGKK110 pKa = 10.65 VGLRR114 pKa = 11.84 AMGVKK119 pKa = 10.0 AA120 pKa = 4.47
Molecular weight: 13.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.216
IPC2_protein 9.853
IPC_protein 9.999
Toseland 10.687
ProMoST 10.204
Dawson 10.774
Bjellqvist 10.379
Wikipedia 10.906
Rodwell 11.403
Grimsley 10.818
Solomon 10.804
Lehninger 10.789
Nozaki 10.643
DTASelect 10.379
Thurlkill 10.657
EMBOSS 11.052
Sillero 10.687
Patrickios 11.125
IPC_peptide 10.818
IPC2_peptide 8.873
IPC2.peptide.svr19 8.73
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
54
0
54
11374
38
1218
210.6
23.66
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.517 ± 1.002
0.44 ± 0.079
6.242 ± 0.435
6.972 ± 0.62
4.185 ± 0.236
6.471 ± 0.417
1.125 ± 0.125
6.91 ± 0.282
8.238 ± 0.501
7.755 ± 0.244
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.558 ± 0.175
5.539 ± 0.415
2.787 ± 0.197
4.422 ± 0.316
3.868 ± 0.331
6.99 ± 0.564
6.348 ± 0.346
6.436 ± 0.384
1.178 ± 0.157
4.018 ± 0.432
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here