Muricauda alvinocaridis
Average proteome isoelectric point is 6.28
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3339 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4S8RQJ5|A0A4S8RQJ5_9FLAO Uncharacterized protein OS=Muricauda alvinocaridis OX=2530200 GN=EZV76_15095 PE=4 SV=1
MM1 pKa = 6.73 NTYY4 pKa = 10.41 ILRR7 pKa = 11.84 LLLLCMLFLCAPSCNQDD24 pKa = 3.24 DD25 pKa = 4.43 TVVDD29 pKa = 4.55 DD30 pKa = 5.92 IINEE34 pKa = 4.17 EE35 pKa = 4.62 PDD37 pKa = 3.95 PDD39 pKa = 4.76 PNPDD43 pKa = 4.46 PDD45 pKa = 5.19 PDD47 pKa = 4.95 PDD49 pKa = 4.97 PDD51 pKa = 3.49 TDD53 pKa = 3.82 KK54 pKa = 11.28 PIFEE58 pKa = 5.27 GDD60 pKa = 3.14 TGNVLVYY67 pKa = 10.6 DD68 pKa = 3.96 EE69 pKa = 5.16 EE70 pKa = 5.77 KK71 pKa = 10.81 IFDD74 pKa = 4.19 GYY76 pKa = 11.58 VLVNDD81 pKa = 4.09 ASDD84 pKa = 3.36 NRR86 pKa = 11.84 VYY88 pKa = 11.57 LMTKK92 pKa = 8.82 EE93 pKa = 3.95 GKK95 pKa = 9.01 IRR97 pKa = 11.84 YY98 pKa = 7.55 EE99 pKa = 3.65 WEE101 pKa = 3.84 LPSNIGNDD109 pKa = 3.63 AEE111 pKa = 4.19 LLEE114 pKa = 4.32 NGKK117 pKa = 9.46 LLVALTDD124 pKa = 3.41 EE125 pKa = 4.2 NAFYY129 pKa = 11.0 DD130 pKa = 3.4 VGGFGGRR137 pKa = 11.84 IQIINPDD144 pKa = 3.74 RR145 pKa = 11.84 SVEE148 pKa = 3.7 WDD150 pKa = 3.52 FLYY153 pKa = 9.91 STEE156 pKa = 5.35 DD157 pKa = 3.8 YY158 pKa = 10.51 ISHH161 pKa = 7.15 HH162 pKa = 7.38 DD163 pKa = 4.57 IEE165 pKa = 4.73 MLPNGNVLILAWQKK179 pKa = 10.7 RR180 pKa = 11.84 SKK182 pKa = 10.84 EE183 pKa = 4.0 DD184 pKa = 3.44 ALQAGYY190 pKa = 10.83 DD191 pKa = 4.04 GIPDD195 pKa = 3.74 NEE197 pKa = 4.2 LLLLEE202 pKa = 4.6 SLIEE206 pKa = 4.12 VNPEE210 pKa = 3.47 NNDD213 pKa = 3.7 IVWEE217 pKa = 4.06 WHH219 pKa = 5.82 SWDD222 pKa = 5.68 HH223 pKa = 7.29 IIQDD227 pKa = 3.89 FDD229 pKa = 3.61 DD230 pKa = 4.37 TKK232 pKa = 11.68 DD233 pKa = 3.44 SFGDD237 pKa = 3.28 IAEE240 pKa = 4.3 NPQLIDD246 pKa = 5.12 LNFHH250 pKa = 7.19 DD251 pKa = 5.84 DD252 pKa = 3.58 DD253 pKa = 6.66 RR254 pKa = 11.84 GDD256 pKa = 3.33 IMHH259 pKa = 7.31 ANGLDD264 pKa = 3.64 YY265 pKa = 11.37 DD266 pKa = 4.2 PVNDD270 pKa = 5.89 LIYY273 pKa = 10.76 LSVNFYY279 pKa = 11.12 SEE281 pKa = 3.91 VWVIDD286 pKa = 3.46 HH287 pKa = 6.2 STTTEE292 pKa = 3.66 EE293 pKa = 4.12 AATNSGGNYY302 pKa = 9.88 NKK304 pKa = 10.79 GGDD307 pKa = 3.43 LVYY310 pKa = 10.85 RR311 pKa = 11.84 FGNPLAYY318 pKa = 9.63 KK319 pKa = 10.4 NEE321 pKa = 4.02 TGTRR325 pKa = 11.84 LFYY328 pKa = 11.12 NNHH331 pKa = 5.8 FPNYY335 pKa = 9.53 LDD337 pKa = 3.57 SDD339 pKa = 4.28 EE340 pKa = 5.2 IGAGNLLIYY349 pKa = 10.43 MNGNNGSQQQSHH361 pKa = 5.75 VYY363 pKa = 9.57 EE364 pKa = 4.58 LNLPDD369 pKa = 5.39 SFTLLPEE376 pKa = 4.34 NDD378 pKa = 3.79 NEE380 pKa = 4.21 PTIEE384 pKa = 4.06 WEE386 pKa = 4.11 FTDD389 pKa = 4.32 AEE391 pKa = 4.65 LYY393 pKa = 10.8 SPIVSGAVRR402 pKa = 11.84 LPNGNTLIAEE412 pKa = 4.11 GRR414 pKa = 11.84 YY415 pKa = 9.91 GYY417 pKa = 10.09 WEE419 pKa = 4.01 VTEE422 pKa = 4.23 SGEE425 pKa = 4.62 VVWLFEE431 pKa = 4.32 GNKK434 pKa = 9.96 LFWRR438 pKa = 11.84 GYY440 pKa = 9.99 SYY442 pKa = 11.23 PKK444 pKa = 10.06 DD445 pKa = 3.61 HH446 pKa = 7.29 PGVLLLEE453 pKa = 4.76 LNN455 pKa = 3.91
Molecular weight: 51.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.71
IPC2_protein 3.859
IPC_protein 3.884
Toseland 3.668
ProMoST 4.024
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.783
Rodwell 3.706
Grimsley 3.567
Solomon 3.859
Lehninger 3.821
Nozaki 3.973
DTASelect 4.202
Thurlkill 3.706
EMBOSS 3.795
Sillero 3.999
Patrickios 1.227
IPC_peptide 3.859
IPC2_peptide 3.973
IPC2.peptide.svr19 3.88
Protein with the highest isoelectric point:
>tr|A0A4S8RUP1|A0A4S8RUP1_9FLAO Ferredoxin--NADP reductase OS=Muricauda alvinocaridis OX=2530200 GN=EZV76_04575 PE=3 SV=1
MM1 pKa = 7.75 KK2 pKa = 10.5 YY3 pKa = 10.47 SPLWRR8 pKa = 11.84 ILWSRR13 pKa = 11.84 FRR15 pKa = 11.84 FPKK18 pKa = 10.0 MPLQLRR24 pKa = 11.84 SGVWLKK30 pKa = 10.67 IPPICHH36 pKa = 6.4 FDD38 pKa = 3.6 MRR40 pKa = 11.84 CGTLTEE46 pKa = 4.92 KK47 pKa = 10.39 SHH49 pKa = 5.99 QLIISHH55 pKa = 6.29 SSFGYY60 pKa = 9.13 SQDD63 pKa = 3.48 RR64 pKa = 11.84 ARR66 pKa = 11.84 NDD68 pKa = 3.27 KK69 pKa = 10.81 KK70 pKa = 10.9 NSKK73 pKa = 9.94
Molecular weight: 8.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.319
IPC2_protein 9.736
IPC_protein 10.204
Toseland 10.774
ProMoST 10.423
Dawson 10.847
Bjellqvist 10.496
Wikipedia 11.008
Rodwell 11.257
Grimsley 10.891
Solomon 10.935
Lehninger 10.921
Nozaki 10.76
DTASelect 10.496
Thurlkill 10.76
EMBOSS 11.155
Sillero 10.774
Patrickios 11.023
IPC_peptide 10.95
IPC2_peptide 9.443
IPC2.peptide.svr19 8.604
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3339
0
3339
1128126
18
4754
337.9
38.01
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.581 ± 0.043
0.744 ± 0.014
5.758 ± 0.039
6.841 ± 0.039
5.101 ± 0.032
6.987 ± 0.042
1.959 ± 0.024
7.085 ± 0.039
7.129 ± 0.059
9.485 ± 0.05
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.436 ± 0.025
5.395 ± 0.043
3.66 ± 0.028
3.599 ± 0.023
3.773 ± 0.031
6.272 ± 0.034
5.614 ± 0.051
6.56 ± 0.032
1.136 ± 0.018
3.869 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here