Campylobacter hominis (strain ATCC BAA-381 / LMG 19568 / NCTC 13146 / CH001A)
Average proteome isoelectric point is 7.15
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1687 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A7I3E6|A7I3E6_CAMHC Histidinol-phosphatase OS=Campylobacter hominis (strain ATCC BAA-381 / LMG 19568 / NCTC 13146 / CH001A) OX=360107 GN=CHAB381_1502 PE=3 SV=1
MM1 pKa = 7.43 AVKK4 pKa = 9.0 ITDD7 pKa = 3.0 ICIACGSCIDD17 pKa = 4.12 EE18 pKa = 4.92 CPVSAIVDD26 pKa = 3.59 DD27 pKa = 4.6 SEE29 pKa = 4.67 NPTGEE34 pKa = 4.35 DD35 pKa = 2.52 IYY37 pKa = 11.57 YY38 pKa = 10.45 VFADD42 pKa = 3.63 KK43 pKa = 10.89 CVEE46 pKa = 4.29 CVGHH50 pKa = 6.61 NDD52 pKa = 3.81 EE53 pKa = 4.8 PACANACPTDD63 pKa = 3.79 GCIVWSDD70 pKa = 3.34 VTAGQPSRR78 pKa = 11.84 KK79 pKa = 9.68 EE80 pKa = 3.32 IGADD84 pKa = 3.31 LRR86 pKa = 11.84 KK87 pKa = 10.29 GDD89 pKa = 4.03 TPVVAA94 pKa = 5.35
Molecular weight: 9.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.867
IPC2_protein 3.948
IPC_protein 3.897
Toseland 3.681
ProMoST 4.075
Dawson 3.897
Bjellqvist 4.05
Wikipedia 3.846
Rodwell 3.732
Grimsley 3.605
Solomon 3.884
Lehninger 3.834
Nozaki 4.012
DTASelect 4.253
Thurlkill 3.745
EMBOSS 3.846
Sillero 4.024
Patrickios 0.299
IPC_peptide 3.872
IPC2_peptide 3.999
IPC2.peptide.svr19 3.917
Protein with the highest isoelectric point:
>sp|A7HZM3|RS5_CAMHC 30S ribosomal protein S5 OS=Campylobacter hominis (strain ATCC BAA-381 / LMG 19568 / NCTC 13146 / CH001A) OX=360107 GN=rpsE PE=3 SV=1
MM1 pKa = 7.59 AKK3 pKa = 10.37 KK4 pKa = 10.93 SMIAKK9 pKa = 9.93 ANRR12 pKa = 11.84 PAKK15 pKa = 9.93 FSSRR19 pKa = 11.84 AYY21 pKa = 8.76 TRR23 pKa = 11.84 CKK25 pKa = 9.61 ICGRR29 pKa = 11.84 PHH31 pKa = 5.66 SVYY34 pKa = 10.37 RR35 pKa = 11.84 DD36 pKa = 3.43 FGICRR41 pKa = 11.84 VCLRR45 pKa = 11.84 KK46 pKa = 9.44 LANEE50 pKa = 4.0 GLIPGLKK57 pKa = 8.97 KK58 pKa = 10.89 ASWW61 pKa = 3.28
Molecular weight: 6.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.324
IPC2_protein 9.78
IPC_protein 10.233
Toseland 10.833
ProMoST 10.409
Dawson 10.906
Bjellqvist 10.57
Wikipedia 11.067
Rodwell 11.33
Grimsley 10.935
Solomon 10.994
Lehninger 10.979
Nozaki 10.833
DTASelect 10.555
Thurlkill 10.818
EMBOSS 11.228
Sillero 10.833
Patrickios 11.096
IPC_peptide 11.008
IPC2_peptide 9.706
IPC2.peptide.svr19 8.528
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1687
0
1687
481493
37
3010
285.4
32.26
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.739 ± 0.06
1.194 ± 0.027
5.635 ± 0.064
6.58 ± 0.078
6.264 ± 0.073
5.99 ± 0.067
1.342 ± 0.021
9.581 ± 0.069
9.792 ± 0.07
9.221 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.216 ± 0.027
6.942 ± 0.089
2.662 ± 0.037
2.403 ± 0.031
3.117 ± 0.041
6.384 ± 0.052
4.321 ± 0.05
5.437 ± 0.05
0.625 ± 0.02
3.556 ± 0.045
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here