Candidatus Nitromaritima sp. SCGC AAA799-C22

Taxonomy: cellular organisms; Bacteria; Nitrospinae/Tectomicrobia group; Nitrospinae; Nitrospinia; Nitrospinales; Nitrospinaceae; Candidatus Nitromaritima

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1350 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0J6YWX5|A0A0J6YWX5_9BACT Uncharacterized protein OS=Candidatus Nitromaritima sp. SCGC AAA799-C22 OX=1628279 GN=UZ36_04360 PE=4 SV=1
MM1 pKa = 7.01STIITDD7 pKa = 3.3EE8 pKa = 4.7CINCGVCEE16 pKa = 4.14PEE18 pKa = 4.7CPNGAISEE26 pKa = 4.21GDD28 pKa = 3.41DD29 pKa = 3.92YY30 pKa = 12.04YY31 pKa = 11.82VIDD34 pKa = 5.43ADD36 pKa = 4.71LCTEE40 pKa = 4.0CVGFHH45 pKa = 6.69GEE47 pKa = 3.94EE48 pKa = 4.28ACQEE52 pKa = 4.17VCPVDD57 pKa = 4.2CCIPDD62 pKa = 3.34EE63 pKa = 4.73DD64 pKa = 4.28NRR66 pKa = 11.84EE67 pKa = 4.14TEE69 pKa = 3.78EE70 pKa = 5.23DD71 pKa = 3.65LLEE74 pKa = 4.29KK75 pKa = 10.47AKK77 pKa = 10.52KK78 pKa = 9.23IHH80 pKa = 6.79SDD82 pKa = 3.54QEE84 pKa = 4.4FPALDD89 pKa = 3.45QLTEE93 pKa = 4.04EE94 pKa = 4.5TSLFRR99 pKa = 11.84NPSRR103 pKa = 11.84KK104 pKa = 9.08NANLL108 pKa = 3.31

Molecular weight: 12.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0J6YVV3|A0A0J6YVV3_9BACT Pyruvate carboxylase subunit B OS=Candidatus Nitromaritima sp. SCGC AAA799-C22 OX=1628279 GN=UZ36_04335 PE=4 SV=1
MM1 pKa = 7.6KK2 pKa = 8.57MTYY5 pKa = 8.55QPKK8 pKa = 8.76NLRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.02RR14 pKa = 11.84THH16 pKa = 6.04GFRR19 pKa = 11.84KK20 pKa = 9.77RR21 pKa = 11.84SSTVGGRR28 pKa = 11.84RR29 pKa = 11.84ILANRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.84GRR39 pKa = 11.84KK40 pKa = 8.9RR41 pKa = 11.84LTVV44 pKa = 3.11

Molecular weight: 5.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1350

0

1350

399800

44

1932

296.1

33.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.386 ± 0.068

1.085 ± 0.023

5.541 ± 0.054

7.052 ± 0.077

4.713 ± 0.063

7.305 ± 0.063

2.074 ± 0.029

6.656 ± 0.054

6.508 ± 0.079

10.045 ± 0.083

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.507 ± 0.033

4.048 ± 0.043

4.482 ± 0.048

3.358 ± 0.036

5.508 ± 0.06

6.041 ± 0.056

5.017 ± 0.055

6.635 ± 0.063

1.148 ± 0.033

2.889 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski