Microbacterium sp. Root53

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Microbacterium; unclassified Microbacterium

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2609 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q7U6K2|A0A0Q7U6K2_9MICO Beta-xylanase OS=Microbacterium sp. Root53 OX=1736553 GN=ASD19_03650 PE=3 SV=1
MM1 pKa = 7.37KK2 pKa = 10.05RR3 pKa = 11.84NKK5 pKa = 9.44IALAGLALLGSGSFVLAGCAGGGGEE30 pKa = 4.51TGGSEE35 pKa = 4.1TPAATTAVITTNGSEE50 pKa = 4.08PQYY53 pKa = 11.04PLIPTMTGEE62 pKa = 4.15TGGGKK67 pKa = 9.32IVDD70 pKa = 4.24SIYY73 pKa = 11.12AGLITYY79 pKa = 7.61EE80 pKa = 4.08ADD82 pKa = 2.95GSVVNEE88 pKa = 3.91VAEE91 pKa = 4.87EE92 pKa = 4.08ITVDD96 pKa = 3.89SPTQLTVKK104 pKa = 10.11LKK106 pKa = 10.8EE107 pKa = 4.03GTTFTNGEE115 pKa = 4.36EE116 pKa = 4.08VTADD120 pKa = 3.38NFTKK124 pKa = 10.32AWNLGAQASNAHH136 pKa = 5.09YY137 pKa = 10.08AAYY140 pKa = 9.84FFEE143 pKa = 6.39DD144 pKa = 3.34IEE146 pKa = 4.5GFSYY150 pKa = 11.32DD151 pKa = 4.15EE152 pKa = 4.81DD153 pKa = 4.46SEE155 pKa = 4.53LTGLEE160 pKa = 4.09VVDD163 pKa = 5.84DD164 pKa = 3.9YY165 pKa = 11.97TFTITLNKK173 pKa = 8.73PASDD177 pKa = 4.0FAQRR181 pKa = 11.84LGYY184 pKa = 9.92SAFYY188 pKa = 9.5PLPDD192 pKa = 3.34VAFDD196 pKa = 4.8DD197 pKa = 5.09IEE199 pKa = 5.46AFGQNPIGNGPYY211 pKa = 9.74MLDD214 pKa = 5.6GEE216 pKa = 5.32DD217 pKa = 3.37AWQHH221 pKa = 5.55DD222 pKa = 3.79VRR224 pKa = 11.84IDD226 pKa = 3.54LVANPDD232 pKa = 3.54YY233 pKa = 10.7EE234 pKa = 4.79GPRR237 pKa = 11.84EE238 pKa = 3.98PMNGGLDD245 pKa = 3.16IVFYY249 pKa = 10.76ASQDD253 pKa = 3.15AAYY256 pKa = 10.95SDD258 pKa = 4.13LQAGNLDD265 pKa = 3.84VLDD268 pKa = 6.05AIPDD272 pKa = 3.95SAFEE276 pKa = 4.3TYY278 pKa = 10.37EE279 pKa = 3.93SDD281 pKa = 4.26LEE283 pKa = 4.29GRR285 pKa = 11.84TVNQAAAIFQSFVIPEE301 pKa = 4.35WIEE304 pKa = 3.88QFSSTTEE311 pKa = 3.59EE312 pKa = 4.18GQLRR316 pKa = 11.84RR317 pKa = 11.84AAISHH322 pKa = 6.97AINRR326 pKa = 11.84EE327 pKa = 3.71EE328 pKa = 3.91VTDD331 pKa = 3.9VVFHH335 pKa = 5.82GTRR338 pKa = 11.84TPASDD343 pKa = 3.36FTSPVIDD350 pKa = 3.69GWSDD354 pKa = 3.5SVPGSEE360 pKa = 4.02VLEE363 pKa = 4.08YY364 pKa = 10.85DD365 pKa = 3.49PEE367 pKa = 4.29KK368 pKa = 11.0AQEE371 pKa = 3.73LWAQAEE377 pKa = 4.36EE378 pKa = 4.02IAPFEE383 pKa = 4.52GEE385 pKa = 3.73FTLAYY390 pKa = 10.15NADD393 pKa = 4.07GGHH396 pKa = 4.87QTWVDD401 pKa = 3.23AVVNQISNTLGITAKK416 pKa = 10.43GDD418 pKa = 3.73PYY420 pKa = 11.15PDD422 pKa = 3.51FATLLDD428 pKa = 3.93ARR430 pKa = 11.84DD431 pKa = 3.77AGTVGPYY438 pKa = 9.37RR439 pKa = 11.84AGWQADD445 pKa = 3.67YY446 pKa = 10.83PGLYY450 pKa = 10.33NFLGPLYY457 pKa = 10.08ATGAGGNDD465 pKa = 3.25GKK467 pKa = 11.25YY468 pKa = 10.63SNPEE472 pKa = 3.28FDD474 pKa = 3.67QLLEE478 pKa = 4.13EE479 pKa = 5.57GISATDD485 pKa = 3.64PEE487 pKa = 4.09EE488 pKa = 4.24AQAKK492 pKa = 8.51FQEE495 pKa = 4.42AQTVLFTDD503 pKa = 4.87LPAIPLWYY511 pKa = 10.7SNVTGGWAAGVEE523 pKa = 4.04NVEE526 pKa = 4.6FGWNSVPLYY535 pKa = 10.72HH536 pKa = 7.61LITKK540 pKa = 10.48AEE542 pKa = 3.92

Molecular weight: 58.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q7UL28|A0A0Q7UL28_9MICO o-succinylbenzoate synthase OS=Microbacterium sp. Root53 OX=1736553 GN=menC PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 10.04KK16 pKa = 8.86HH17 pKa = 4.36GFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILAARR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.31GRR40 pKa = 11.84AEE42 pKa = 3.92LSAA45 pKa = 4.93

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2609

0

2609

838976

38

2051

321.6

34.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.068 ± 0.085

0.501 ± 0.013

6.27 ± 0.044

6.248 ± 0.042

3.003 ± 0.031

9.048 ± 0.045

2.079 ± 0.021

4.433 ± 0.033

1.919 ± 0.039

9.998 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.802 ± 0.021

1.715 ± 0.023

5.573 ± 0.039

2.685 ± 0.029

7.788 ± 0.054

4.951 ± 0.024

5.613 ± 0.033

8.859 ± 0.039

1.496 ± 0.021

1.948 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski