Amino acid dipepetide frequency for Microbacterium sp. Root53

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.897AlaAla: 21.897 ± 0.307	0.706AlaCys: 0.706 ± 0.036	8.714AlaAsp: 8.714 ± 0.121	9.507AlaGlu: 9.507 ± 0.153	3.982AlaPhe: 3.982 ± 0.077	12.571AlaGly: 12.571 ± 0.136	2.844AlaHis: 2.844 ± 0.067	6.018AlaIle: 6.018 ± 0.093	2.77AlaLys: 2.77 ± 0.08	14.799AlaLeu: 14.799 ± 0.175	2.828AlaMet: 2.828 ± 0.057	2.114AlaAsn: 2.114 ± 0.052	7.27AlaPro: 7.27 ± 0.139	3.887AlaGln: 3.887 ± 0.069	10.617AlaArg: 10.617 ± 0.143	6.596AlaSer: 6.596 ± 0.096	7.068AlaThr: 7.068 ± 0.107	11.905AlaVal: 11.905 ± 0.167	2.07AlaTrp: 2.07 ± 0.051	2.517AlaTyr: 2.517 ± 0.053	0.0AlaXaa: 0.0 ± 0.0
Cys	0.715CysAla: 0.715 ± 0.033	0.055CysCys: 0.055 ± 0.009	0.331CysAsp: 0.331 ± 0.02	0.268CysGlu: 0.268 ± 0.017	0.18CysPhe: 0.18 ± 0.016	0.573CysGly: 0.573 ± 0.029	0.117CysHis: 0.117 ± 0.011	0.167CysIle: 0.167 ± 0.013	0.057CysLys: 0.057 ± 0.007	0.434CysLeu: 0.434 ± 0.025	0.079CysMet: 0.079 ± 0.01	0.089CysAsn: 0.089 ± 0.01	0.279CysPro: 0.279 ± 0.019	0.131CysGln: 0.131 ± 0.015	0.398CysArg: 0.398 ± 0.026	0.275CysSer: 0.275 ± 0.018	0.293CysThr: 0.293 ± 0.017	0.403CysVal: 0.403 ± 0.025	0.063CysTrp: 0.063 ± 0.009	0.101CysTyr: 0.101 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	9.469AspAla: 9.469 ± 0.113	0.224AspCys: 0.224 ± 0.017	4.161AspAsp: 4.161 ± 0.08	4.879AspGlu: 4.879 ± 0.088	1.712AspPhe: 1.712 ± 0.052	6.253AspGly: 6.253 ± 0.113	1.217AspHis: 1.217 ± 0.038	2.458AspIle: 2.458 ± 0.053	0.923AspLys: 0.923 ± 0.039	6.065AspLeu: 6.065 ± 0.079	0.906AspMet: 0.906 ± 0.034	0.793AspAsn: 0.793 ± 0.033	4.421AspPro: 4.421 ± 0.076	1.509AspGln: 1.509 ± 0.042	4.767AspArg: 4.767 ± 0.094	2.129AspSer: 2.129 ± 0.056	2.75AspThr: 2.75 ± 0.064	5.95AspVal: 5.95 ± 0.083	0.946AspTrp: 0.946 ± 0.037	1.17AspTyr: 1.17 ± 0.042	0.0AspXaa: 0.0 ± 0.0
Glu	8.404GluAla: 8.404 ± 0.135	0.26GluCys: 0.26 ± 0.018	3.224GluAsp: 3.224 ± 0.066	3.573GluGlu: 3.573 ± 0.081	1.874GluPhe: 1.874 ± 0.052	4.738GluGly: 4.738 ± 0.074	1.645GluHis: 1.645 ± 0.043	2.96GluIle: 2.96 ± 0.069	1.473GluLys: 1.473 ± 0.056	7.001GluLeu: 7.001 ± 0.098	0.936GluMet: 0.936 ± 0.039	1.179GluAsn: 1.179 ± 0.039	3.591GluPro: 3.591 ± 0.09	2.334GluGln: 2.334 ± 0.064	6.026GluArg: 6.026 ± 0.111	2.801GluSer: 2.801 ± 0.057	3.3GluThr: 3.3 ± 0.066	4.935GluVal: 4.935 ± 0.083	0.994GluTrp: 0.994 ± 0.036	1.229GluTyr: 1.229 ± 0.041	0.0GluXaa: 0.0 ± 0.0
Phe	4.458PheAla: 4.458 ± 0.079	0.154PheCys: 0.154 ± 0.013	2.362PheAsp: 2.362 ± 0.052	1.896PheGlu: 1.896 ± 0.047	1.0PhePhe: 1.0 ± 0.038	3.312PheGly: 3.312 ± 0.066	0.58PheHis: 0.58 ± 0.026	1.021PheIle: 1.021 ± 0.036	0.483PheLys: 0.483 ± 0.027	2.689PheLeu: 2.689 ± 0.06	0.48PheMet: 0.48 ± 0.027	0.611PheAsn: 0.611 ± 0.032	1.381PhePro: 1.381 ± 0.045	0.744PheGln: 0.744 ± 0.034	1.805PheArg: 1.805 ± 0.046	1.499PheSer: 1.499 ± 0.048	1.915PheThr: 1.915 ± 0.051	2.578PheVal: 2.578 ± 0.062	0.448PheTrp: 0.448 ± 0.023	0.613PheTyr: 0.613 ± 0.03	0.0PheXaa: 0.0 ± 0.0
Gly	11.586GlyAla: 11.586 ± 0.143	0.555GlyCys: 0.555 ± 0.026	5.398GlyAsp: 5.398 ± 0.097	5.657GlyGlu: 5.657 ± 0.087	3.199GlyPhe: 3.199 ± 0.069	7.838GlyGly: 7.838 ± 0.12	1.867GlyHis: 1.867 ± 0.05	4.55GlyIle: 4.55 ± 0.084	1.973GlyLys: 1.973 ± 0.054	8.742GlyLeu: 8.742 ± 0.117	2.029GlyMet: 2.029 ± 0.054	1.488GlyAsn: 1.488 ± 0.053	3.998GlyPro: 3.998 ± 0.068	2.355GlyGln: 2.355 ± 0.055	7.056GlyArg: 7.056 ± 0.097	4.899GlySer: 4.899 ± 0.086	5.147GlyThr: 5.147 ± 0.076	8.23GlyVal: 8.23 ± 0.115	1.701GlyTrp: 1.701 ± 0.049	2.213GlyTyr: 2.213 ± 0.063	0.001GlyXaa: 0.001 ± 0.001
His	2.656HisAla: 2.656 ± 0.062	0.132HisCys: 0.132 ± 0.014	1.348HisAsp: 1.348 ± 0.041	1.279HisGlu: 1.279 ± 0.039	0.563HisPhe: 0.563 ± 0.022	2.19HisGly: 2.19 ± 0.051	0.628HisHis: 0.628 ± 0.029	0.778HisIle: 0.778 ± 0.033	0.272HisLys: 0.272 ± 0.018	2.025HisLeu: 2.025 ± 0.054	0.35HisMet: 0.35 ± 0.022	0.325HisAsn: 0.325 ± 0.022	1.6HisPro: 1.6 ± 0.041	0.433HisGln: 0.433 ± 0.025	1.715HisArg: 1.715 ± 0.048	0.902HisSer: 0.902 ± 0.032	1.021HisThr: 1.021 ± 0.034	1.864HisVal: 1.864 ± 0.056	0.285HisTrp: 0.285 ± 0.019	0.426HisTyr: 0.426 ± 0.024	0.002HisXaa: 0.002 ± 0.002
Ile	7.171IleAla: 7.171 ± 0.094	0.218IleCys: 0.218 ± 0.015	3.454IleAsp: 3.454 ± 0.061	3.15IleGlu: 3.15 ± 0.066	0.998IlePhe: 0.998 ± 0.04	4.618IleGly: 4.618 ± 0.086	0.751IleHis: 0.751 ± 0.033	1.49IleIle: 1.49 ± 0.049	0.765IleLys: 0.765 ± 0.034	3.726IleLeu: 3.726 ± 0.072	0.6IleMet: 0.6 ± 0.027	0.769IleAsn: 0.769 ± 0.037	2.34IlePro: 2.34 ± 0.059	1.03IleGln: 1.03 ± 0.033	3.058IleArg: 3.058 ± 0.07	1.991IleSer: 1.991 ± 0.053	2.477IleThr: 2.477 ± 0.057	4.491IleVal: 4.491 ± 0.079	0.474IleTrp: 0.474 ± 0.023	0.759IleTyr: 0.759 ± 0.033	0.0IleXaa: 0.0 ± 0.0
Lys	2.538LysAla: 2.538 ± 0.073	0.069LysCys: 0.069 ± 0.009	1.067LysAsp: 1.067 ± 0.044	0.906LysGlu: 0.906 ± 0.037	0.404LysPhe: 0.404 ± 0.023	1.626LysGly: 1.626 ± 0.052	0.486LysHis: 0.486 ± 0.024	0.818LysIle: 0.818 ± 0.037	0.732LysLys: 0.732 ± 0.037	1.9LysLeu: 1.9 ± 0.053	0.353LysMet: 0.353 ± 0.022	0.442LysAsn: 0.442 ± 0.028	1.212LysPro: 1.212 ± 0.042	0.675LysGln: 0.675 ± 0.032	1.524LysArg: 1.524 ± 0.05	0.956LysSer: 0.956 ± 0.038	1.193LysThr: 1.193 ± 0.05	1.66LysVal: 1.66 ± 0.05	0.215LysTrp: 0.215 ± 0.017	0.411LysTyr: 0.411 ± 0.025	0.0LysXaa: 0.0 ± 0.0
Leu	15.337LeuAla: 15.337 ± 0.185	0.511LeuCys: 0.511 ± 0.027	6.568LeuAsp: 6.568 ± 0.103	5.938LeuGlu: 5.938 ± 0.095	2.714LeuPhe: 2.714 ± 0.069	9.139LeuGly: 9.139 ± 0.12	1.886LeuHis: 1.886 ± 0.055	4.327LeuIle: 4.327 ± 0.09	1.751LeuLys: 1.751 ± 0.049	9.708LeuLeu: 9.708 ± 0.143	1.701LeuMet: 1.701 ± 0.048	1.595LeuAsn: 1.595 ± 0.047	5.582LeuPro: 5.582 ± 0.095	2.463LeuGln: 2.463 ± 0.049	7.86LeuArg: 7.86 ± 0.111	5.231LeuSer: 5.231 ± 0.077	5.865LeuThr: 5.865 ± 0.085	8.951LeuVal: 8.951 ± 0.124	1.279LeuTrp: 1.279 ± 0.044	1.575LeuTyr: 1.575 ± 0.043	0.001LeuXaa: 0.001 ± 0.001
Met	2.24MetAla: 2.24 ± 0.054	0.087MetCys: 0.087 ± 0.011	0.853MetAsp: 0.853 ± 0.031	0.648MetGlu: 0.648 ± 0.031	0.518MetPhe: 0.518 ± 0.023	1.399MetGly: 1.399 ± 0.048	0.369MetHis: 0.369 ± 0.021	0.834MetIle: 0.834 ± 0.034	0.423MetLys: 0.423 ± 0.025	1.919MetLeu: 1.919 ± 0.046	0.311MetMet: 0.311 ± 0.02	0.427MetAsn: 0.427 ± 0.021	1.184MetPro: 1.184 ± 0.038	0.502MetGln: 0.502 ± 0.024	1.499MetArg: 1.499 ± 0.037	1.361MetSer: 1.361 ± 0.042	1.659MetThr: 1.659 ± 0.047	1.273MetVal: 1.273 ± 0.042	0.234MetTrp: 0.234 ± 0.018	0.281MetTyr: 0.281 ± 0.019	0.0MetXaa: 0.0 ± 0.0
Asn	2.225AsnAla: 2.225 ± 0.051	0.095AsnCys: 0.095 ± 0.011	0.931AsnAsp: 0.931 ± 0.036	0.971AsnGlu: 0.971 ± 0.037	0.504AsnPhe: 0.504 ± 0.028	1.653AsnGly: 1.653 ± 0.055	0.35AsnHis: 0.35 ± 0.021	0.729AsnIle: 0.729 ± 0.033	0.342AsnLys: 0.342 ± 0.023	1.718AsnLeu: 1.718 ± 0.049	0.261AsnMet: 0.261 ± 0.02	0.374AsnAsn: 0.374 ± 0.023	1.452AsnPro: 1.452 ± 0.041	0.507AsnGln: 0.507 ± 0.028	1.198AsnArg: 1.198 ± 0.043	0.762AsnSer: 0.762 ± 0.027	0.937AsnThr: 0.937 ± 0.038	1.503AsnVal: 1.503 ± 0.042	0.246AsnTrp: 0.246 ± 0.019	0.396AsnTyr: 0.396 ± 0.021	0.0AsnXaa: 0.0 ± 0.0
Pro	7.96ProAla: 7.96 ± 0.139	0.219ProCys: 0.219 ± 0.017	4.181ProAsp: 4.181 ± 0.079	4.399ProGlu: 4.399 ± 0.078	1.682ProPhe: 1.682 ± 0.045	5.359ProGly: 5.359 ± 0.083	1.216ProHis: 1.216 ± 0.043	2.118ProIle: 2.118 ± 0.042	0.987ProLys: 0.987 ± 0.035	5.026ProLeu: 5.026 ± 0.09	0.894ProMet: 0.894 ± 0.038	0.927ProAsn: 0.927 ± 0.037	2.442ProPro: 2.442 ± 0.075	1.501ProGln: 1.501 ± 0.045	3.726ProArg: 3.726 ± 0.078	2.902ProSer: 2.902 ± 0.055	3.062ProThr: 3.062 ± 0.072	5.121ProVal: 5.121 ± 0.079	0.884ProTrp: 0.884 ± 0.036	1.125ProTyr: 1.125 ± 0.042	0.001ProXaa: 0.001 ± 0.001
Gln	3.561GlnAla: 3.561 ± 0.074	0.119GlnCys: 0.119 ± 0.011	1.375GlnAsp: 1.375 ± 0.044	1.327GlnGlu: 1.327 ± 0.045	0.741GlnPhe: 0.741 ± 0.032	2.155GlnGly: 2.155 ± 0.046	0.644GlnHis: 0.644 ± 0.029	1.31GlnIle: 1.31 ± 0.039	0.647GlnLys: 0.647 ± 0.032	3.1GlnLeu: 3.1 ± 0.063	0.48GlnMet: 0.48 ± 0.024	0.549GlnAsn: 0.549 ± 0.027	1.446GlnPro: 1.446 ± 0.046	1.05GlnGln: 1.05 ± 0.042	2.337GlnArg: 2.337 ± 0.056	1.283GlnSer: 1.283 ± 0.043	1.468GlnThr: 1.468 ± 0.044	2.266GlnVal: 2.266 ± 0.051	0.422GlnTrp: 0.422 ± 0.019	0.572GlnTyr: 0.572 ± 0.026	0.0GlnXaa: 0.0 ± 0.0
Arg	10.177ArgAla: 10.177 ± 0.157	0.355ArgCys: 0.355 ± 0.023	4.733ArgAsp: 4.733 ± 0.083	5.094ArgGlu: 5.094 ± 0.097	2.566ArgPhe: 2.566 ± 0.053	6.138ArgGly: 6.138 ± 0.097	1.721ArgHis: 1.721 ± 0.05	4.095ArgIle: 4.095 ± 0.064	1.318ArgLys: 1.318 ± 0.044	7.767ArgLeu: 7.767 ± 0.114	1.865ArgMet: 1.865 ± 0.041	1.255ArgAsn: 1.255 ± 0.036	4.076ArgPro: 4.076 ± 0.085	1.919ArgGln: 1.919 ± 0.048	7.37ArgArg: 7.37 ± 0.12	3.873ArgSer: 3.873 ± 0.072	4.346ArgThr: 4.346 ± 0.076	6.455ArgVal: 6.455 ± 0.092	1.18ArgTrp: 1.18 ± 0.042	1.572ArgTyr: 1.572 ± 0.048	0.002ArgXaa: 0.002 ± 0.002
Ser	6.377SerAla: 6.377 ± 0.088	0.253SerCys: 0.253 ± 0.018	2.844SerAsp: 2.844 ± 0.064	2.721SerGlu: 2.721 ± 0.059	1.664SerPhe: 1.664 ± 0.046	5.128SerGly: 5.128 ± 0.08	0.965SerHis: 0.965 ± 0.036	2.299SerIle: 2.299 ± 0.052	0.98SerLys: 0.98 ± 0.043	4.788SerLeu: 4.788 ± 0.07	1.036SerMet: 1.036 ± 0.032	0.926SerAsn: 0.926 ± 0.032	2.887SerPro: 2.887 ± 0.062	1.244SerGln: 1.244 ± 0.044	3.697SerArg: 3.697 ± 0.07	2.824SerSer: 2.824 ± 0.063	2.958SerThr: 2.958 ± 0.066	4.085SerVal: 4.085 ± 0.076	0.827SerTrp: 0.827 ± 0.03	1.006SerTyr: 1.006 ± 0.036	0.001SerXaa: 0.001 ± 0.001
Thr	7.473ThrAla: 7.473 ± 0.114	0.286ThrCys: 0.286 ± 0.02	3.128ThrAsp: 3.128 ± 0.058	3.036ThrGlu: 3.036 ± 0.065	1.72ThrPhe: 1.72 ± 0.051	5.649ThrGly: 5.649 ± 0.082	1.126ThrHis: 1.126 ± 0.037	2.69ThrIle: 2.69 ± 0.057	0.998ThrLys: 0.998 ± 0.041	5.663ThrLeu: 5.663 ± 0.081	0.859ThrMet: 0.859 ± 0.027	0.896ThrAsn: 0.896 ± 0.037	3.895ThrPro: 3.895 ± 0.072	1.402ThrGln: 1.402 ± 0.04	3.787ThrArg: 3.787 ± 0.069	2.888ThrSer: 2.888 ± 0.059	3.241ThrThr: 3.241 ± 0.077	5.353ThrVal: 5.353 ± 0.096	0.892ThrTrp: 0.892 ± 0.035	1.149ThrTyr: 1.149 ± 0.034	0.002ThrXaa: 0.002 ± 0.002
Val	12.273ValAla: 12.273 ± 0.131	0.496ValCys: 0.496 ± 0.023	5.8ValAsp: 5.8 ± 0.095	5.312ValGlu: 5.312 ± 0.088	2.759ValPhe: 2.759 ± 0.067	7.009ValGly: 7.009 ± 0.111	1.724ValHis: 1.724 ± 0.052	4.147ValIle: 4.147 ± 0.074	1.684ValLys: 1.684 ± 0.05	9.172ValLeu: 9.172 ± 0.105	1.441ValMet: 1.441 ± 0.045	1.603ValAsn: 1.603 ± 0.046	4.993ValPro: 4.993 ± 0.089	2.08ValGln: 2.08 ± 0.053	6.496ValArg: 6.496 ± 0.1	4.567ValSer: 4.567 ± 0.086	5.396ValThr: 5.396 ± 0.082	8.857ValVal: 8.857 ± 0.127	1.154ValTrp: 1.154 ± 0.042	1.626ValTyr: 1.626 ± 0.044	0.001ValXaa: 0.001 ± 0.001
Trp	1.665TrpAla: 1.665 ± 0.046	0.104TrpCys: 0.104 ± 0.011	0.843TrpAsp: 0.843 ± 0.033	0.672TrpGlu: 0.672 ± 0.028	0.561TrpPhe: 0.561 ± 0.03	1.131TrpGly: 1.131 ± 0.042	0.369TrpHis: 0.369 ± 0.021	0.847TrpIle: 0.847 ± 0.033	0.285TrpLys: 0.285 ± 0.023	1.731TrpLeu: 1.731 ± 0.06	0.327TrpMet: 0.327 ± 0.021	0.393TrpAsn: 0.393 ± 0.022	0.696TrpPro: 0.696 ± 0.028	0.552TrpGln: 0.552 ± 0.025	1.352TrpArg: 1.352 ± 0.047	0.795TrpSer: 0.795 ± 0.033	0.896TrpThr: 0.896 ± 0.038	1.093TrpVal: 1.093 ± 0.037	0.356TrpTrp: 0.356 ± 0.02	0.288TrpTyr: 0.288 ± 0.018	0.001TrpXaa: 0.001 ± 0.001
Tyr	2.499TyrAla: 2.499 ± 0.055	0.11TyrCys: 0.11 ± 0.011	1.381TyrAsp: 1.381 ± 0.038	1.241TyrGlu: 1.241 ± 0.044	0.69TyrPhe: 0.69 ± 0.029	2.002TyrGly: 2.002 ± 0.059	0.288TyrHis: 0.288 ± 0.018	0.672TyrIle: 0.672 ± 0.032	0.323TyrLys: 0.323 ± 0.022	2.007TyrLeu: 2.007 ± 0.051	0.286TyrMet: 0.286 ± 0.018	0.397TyrAsn: 0.397 ± 0.025	0.988TyrPro: 0.988 ± 0.037	0.536TyrGln: 0.536 ± 0.025	1.59TyrArg: 1.59 ± 0.044	0.975TyrSer: 0.975 ± 0.038	1.138TyrThr: 1.138 ± 0.037	1.616TyrVal: 1.616 ± 0.044	0.288TyrTrp: 0.288 ± 0.022	0.448TyrTyr: 0.448 ± 0.024	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.001XaaCys: 0.001 ± 0.001	0.001XaaAsp: 0.001 ± 0.001	0.001XaaGlu: 0.001 ± 0.001	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.001XaaLys: 0.001 ± 0.001	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.001XaaPro: 0.001 ± 0.001	0.002XaaGln: 0.002 ± 0.002	0.001XaaArg: 0.001 ± 0.001	0.001XaaSer: 0.001 ± 0.001	0.0XaaThr: 0.0 ± 0.0	0.001XaaVal: 0.001 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2609 proteins (838976 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---