Streptococcus sp. UMB1385
Average proteome isoelectric point is 6.51
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1829 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2N6S842|A0A2N6S842_9STRE Aminotransferase class V-fold PLP-dependent enzyme OS=Streptococcus sp. UMB1385 OX=2069309 GN=CJ217_00750 PE=3 SV=1
MM1 pKa = 7.8 ANTDD5 pKa = 3.4 NQIILITDD13 pKa = 3.53 VAVGKK18 pKa = 9.14 FQEE21 pKa = 4.27 MLFDD25 pKa = 4.4 ADD27 pKa = 5.19 KK28 pKa = 10.81 RR29 pKa = 11.84 DD30 pKa = 3.59 SYY32 pKa = 11.72 LKK34 pKa = 10.41 ISIALEE40 pKa = 4.2 GTQMHH45 pKa = 6.1 YY46 pKa = 10.82 AIDD49 pKa = 3.63 IKK51 pKa = 11.34 DD52 pKa = 3.65 EE53 pKa = 4.56 LEE55 pKa = 4.35 VGDD58 pKa = 4.25 KK59 pKa = 10.55 VLNFGDD65 pKa = 4.21 LKK67 pKa = 11.3 VLVNEE72 pKa = 4.23 EE73 pKa = 4.75 DD74 pKa = 3.66 EE75 pKa = 4.92 LLLKK79 pKa = 10.62 GLEE82 pKa = 3.72 IDD84 pKa = 4.13 YY85 pKa = 11.15 VQDD88 pKa = 3.48 EE89 pKa = 4.71 YY90 pKa = 11.83 GSEE93 pKa = 4.2 FTLHH97 pKa = 6.14 NPNMIEE103 pKa = 4.6 IYY105 pKa = 10.39 DD106 pKa = 4.39 DD107 pKa = 4.02 EE108 pKa = 7.58 DD109 pKa = 4.4 GGCCGNGCCCGG120 pKa = 3.43
Molecular weight: 13.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.727
IPC2_protein 3.884
IPC_protein 3.859
Toseland 3.656
ProMoST 4.024
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.77
Rodwell 3.694
Grimsley 3.567
Solomon 3.834
Lehninger 3.795
Nozaki 3.961
DTASelect 4.177
Thurlkill 3.706
EMBOSS 3.783
Sillero 3.986
Patrickios 1.036
IPC_peptide 3.834
IPC2_peptide 3.961
IPC2.peptide.svr19 3.856
Protein with the highest isoelectric point:
>tr|A0A2N6S708|A0A2N6S708_9STRE Histidine kinase OS=Streptococcus sp. UMB1385 OX=2069309 GN=CJ217_03485 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.42 RR3 pKa = 11.84 TYY5 pKa = 10.24 QPNKK9 pKa = 8.16 RR10 pKa = 11.84 KK11 pKa = 9.54 HH12 pKa = 5.99 SKK14 pKa = 8.91 VHH16 pKa = 5.68 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MSTKK25 pKa = 10.08 NGRR28 pKa = 11.84 NVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.94 GRR39 pKa = 11.84 KK40 pKa = 8.67 VLSAA44 pKa = 4.05
Molecular weight: 5.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.39
IPC2_protein 11.052
IPC_protein 12.398
Toseland 12.559
ProMoST 13.042
Dawson 12.559
Bjellqvist 12.544
Wikipedia 13.027
Rodwell 12.34
Grimsley 12.603
Solomon 13.056
Lehninger 12.954
Nozaki 12.559
DTASelect 12.544
Thurlkill 12.559
EMBOSS 13.056
Sillero 12.559
Patrickios 12.076
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 9.006
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1829
0
1829
588639
23
3507
321.8
36.32
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.163 ± 0.134
0.678 ± 0.02
5.65 ± 0.059
7.754 ± 0.074
4.506 ± 0.065
6.148 ± 0.068
1.44 ± 0.021
8.449 ± 0.103
9.001 ± 0.072
9.253 ± 0.106
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.166 ± 0.041
6.079 ± 0.061
2.909 ± 0.062
2.679 ± 0.035
3.4 ± 0.045
6.016 ± 0.055
5.612 ± 0.086
7.214 ± 0.067
0.636 ± 0.018
4.249 ± 0.053
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here