Amino acid dipepetide frequency for Streptococcus sp. UMB1385

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.402AlaAla: 4.402 ± 0.176
0.432AlaCys: 0.432 ± 0.033
3.126AlaAsp: 3.126 ± 0.112
4.104AlaGlu: 4.104 ± 0.118
2.339AlaPhe: 2.339 ± 0.077
4.191AlaGly: 4.191 ± 0.11
0.885AlaHis: 0.885 ± 0.039
5.492AlaIle: 5.492 ± 0.162
6.114AlaLys: 6.114 ± 0.423
5.817AlaLeu: 5.817 ± 0.126
1.434AlaMet: 1.434 ± 0.059
3.292AlaAsn: 3.292 ± 0.085
1.646AlaPro: 1.646 ± 0.097
1.792AlaGln: 1.792 ± 0.062
2.222AlaArg: 2.222 ± 0.064
3.42AlaSer: 3.42 ± 0.065
3.919AlaThr: 3.919 ± 0.133
4.425AlaVal: 4.425 ± 0.119
0.445AlaTrp: 0.445 ± 0.039
2.132AlaTyr: 2.132 ± 0.065
0.0AlaXaa: 0.0 ± 0.0
Cys
0.409CysAla: 0.409 ± 0.028
0.121CysCys: 0.121 ± 0.015
0.455CysAsp: 0.455 ± 0.032
0.465CysGlu: 0.465 ± 0.034
0.33CysPhe: 0.33 ± 0.026
0.579CysGly: 0.579 ± 0.034
0.143CysHis: 0.143 ± 0.018
0.545CysIle: 0.545 ± 0.03
0.474CysLys: 0.474 ± 0.031
0.617CysLeu: 0.617 ± 0.038
0.138CysMet: 0.138 ± 0.015
0.311CysAsn: 0.311 ± 0.021
0.224CysPro: 0.224 ± 0.02
0.175CysGln: 0.175 ± 0.018
0.224CysArg: 0.224 ± 0.018
0.494CysSer: 0.494 ± 0.03
0.316CysThr: 0.316 ± 0.024
0.438CysVal: 0.438 ± 0.026
0.037CysTrp: 0.037 ± 0.007
0.28CysTyr: 0.28 ± 0.021
0.0CysXaa: 0.0 ± 0.0
Asp
3.294AspAla: 3.294 ± 0.227
0.357AspCys: 0.357 ± 0.026
2.64AspAsp: 2.64 ± 0.074
4.845AspGlu: 4.845 ± 0.103
3.036AspPhe: 3.036 ± 0.083
3.671AspGly: 3.671 ± 0.206
0.674AspHis: 0.674 ± 0.032
5.063AspIle: 5.063 ± 0.114
5.883AspLys: 5.883 ± 0.116
4.757AspLeu: 4.757 ± 0.114
1.193AspMet: 1.193 ± 0.046
3.554AspAsn: 3.554 ± 0.102
1.374AspPro: 1.374 ± 0.088
1.045AspGln: 1.045 ± 0.051
1.949AspArg: 1.949 ± 0.065
3.202AspSer: 3.202 ± 0.074
2.749AspThr: 2.749 ± 0.1
3.991AspVal: 3.991 ± 0.084
0.399AspTrp: 0.399 ± 0.028
2.829AspTyr: 2.829 ± 0.079
0.0AspXaa: 0.0 ± 0.0
Glu
4.51GluAla: 4.51 ± 0.12
0.494GluCys: 0.494 ± 0.033
4.364GluAsp: 4.364 ± 0.1
7.297GluGlu: 7.297 ± 0.164
3.255GluPhe: 3.255 ± 0.085
3.858GluGly: 3.858 ± 0.113
1.154GluHis: 1.154 ± 0.055
6.343GluIle: 6.343 ± 0.143
8.732GluLys: 8.732 ± 0.174
7.341GluLeu: 7.341 ± 0.146
1.67GluMet: 1.67 ± 0.061
5.368GluAsn: 5.368 ± 0.118
1.432GluPro: 1.432 ± 0.062
2.521GluGln: 2.521 ± 0.074
2.643GluArg: 2.643 ± 0.085
3.35GluSer: 3.35 ± 0.081
3.52GluThr: 3.52 ± 0.107
5.997GluVal: 5.997 ± 0.137
0.42GluTrp: 0.42 ± 0.029
3.269GluTyr: 3.269 ± 0.089
0.0GluXaa: 0.0 ± 0.0
Phe
2.524PheAla: 2.524 ± 0.078
0.387PheCys: 0.387 ± 0.026
2.769PheAsp: 2.769 ± 0.075
3.211PheGlu: 3.211 ± 0.089
2.135PhePhe: 2.135 ± 0.078
2.93PheGly: 2.93 ± 0.081
0.564PheHis: 0.564 ± 0.033
4.126PheIle: 4.126 ± 0.129
3.525PheLys: 3.525 ± 0.072
4.327PheLeu: 4.327 ± 0.11
1.029PheMet: 1.029 ± 0.051
2.754PheAsn: 2.754 ± 0.076
1.113PhePro: 1.113 ± 0.047
0.905PheGln: 0.905 ± 0.037
1.279PheArg: 1.279 ± 0.046
3.496PheSer: 3.496 ± 0.105
2.44PheThr: 2.44 ± 0.076
3.172PheVal: 3.172 ± 0.076
0.338PheTrp: 0.338 ± 0.023
2.032PheTyr: 2.032 ± 0.069
0.0PheXaa: 0.0 ± 0.0
Gly
4.21GlyAla: 4.21 ± 0.122
0.506GlyCys: 0.506 ± 0.034
3.112GlyAsp: 3.112 ± 0.091
4.274GlyGlu: 4.274 ± 0.115
2.837GlyPhe: 2.837 ± 0.085
4.023GlyGly: 4.023 ± 0.11
1.011GlyHis: 1.011 ± 0.04
5.304GlyIle: 5.304 ± 0.119
5.241GlyLys: 5.241 ± 0.145
5.723GlyLeu: 5.723 ± 0.132
1.486GlyMet: 1.486 ± 0.059
3.111GlyAsn: 3.111 ± 0.115
1.062GlyPro: 1.062 ± 0.042
1.687GlyGln: 1.687 ± 0.081
2.254GlyArg: 2.254 ± 0.092
3.53GlySer: 3.53 ± 0.095
3.715GlyThr: 3.715 ± 0.147
4.913GlyVal: 4.913 ± 0.099
0.454GlyTrp: 0.454 ± 0.032
3.024GlyTyr: 3.024 ± 0.073
0.0GlyXaa: 0.0 ± 0.0
His
0.81HisAla: 0.81 ± 0.044
0.127HisCys: 0.127 ± 0.017
0.766HisAsp: 0.766 ± 0.036
0.968HisGlu: 0.968 ± 0.046
0.707HisPhe: 0.707 ± 0.037
1.033HisGly: 1.033 ± 0.043
0.301HisHis: 0.301 ± 0.029
1.228HisIle: 1.228 ± 0.049
1.046HisLys: 1.046 ± 0.044
1.308HisLeu: 1.308 ± 0.052
0.291HisMet: 0.291 ± 0.019
0.865HisAsn: 0.865 ± 0.045
0.64HisPro: 0.64 ± 0.037
0.347HisGln: 0.347 ± 0.027
0.608HisArg: 0.608 ± 0.038
0.87HisSer: 0.87 ± 0.039
0.736HisThr: 0.736 ± 0.038
0.922HisVal: 0.922 ± 0.041
0.095HisTrp: 0.095 ± 0.013
0.734HisTyr: 0.734 ± 0.036
0.0HisXaa: 0.0 ± 0.0
Ile
5.569IleAla: 5.569 ± 0.123
0.7IleCys: 0.7 ± 0.037
5.363IleAsp: 5.363 ± 0.172
6.163IleGlu: 6.163 ± 0.125
4.028IlePhe: 4.028 ± 0.117
4.886IleGly: 4.886 ± 0.108
1.174IleHis: 1.174 ± 0.045
7.208IleIle: 7.208 ± 0.167
7.003IleLys: 7.003 ± 0.143
7.954IleLeu: 7.954 ± 0.175
1.763IleMet: 1.763 ± 0.062
4.84IleAsn: 4.84 ± 0.114
2.835IlePro: 2.835 ± 0.079
2.096IleGln: 2.096 ± 0.073
2.628IleArg: 2.628 ± 0.081
5.907IleSer: 5.907 ± 0.129
4.803IleThr: 4.803 ± 0.114
6.046IleVal: 6.046 ± 0.108
0.454IleTrp: 0.454 ± 0.031
3.07IleTyr: 3.07 ± 0.072
0.0IleXaa: 0.0 ± 0.0
Lys
5.144LysAla: 5.144 ± 0.289
0.33LysCys: 0.33 ± 0.027
5.946LysAsp: 5.946 ± 0.135
9.062LysGlu: 9.062 ± 0.168
3.432LysPhe: 3.432 ± 0.078
4.286LysGly: 4.286 ± 0.131
1.244LysHis: 1.244 ± 0.049
7.297LysIle: 7.297 ± 0.142
8.234LysLys: 8.234 ± 0.158
8.141LysLeu: 8.141 ± 0.156
2.256LysMet: 2.256 ± 0.081
6.204LysAsn: 6.204 ± 0.128
2.47LysPro: 2.47 ± 0.121
2.852LysGln: 2.852 ± 0.069
2.871LysArg: 2.871 ± 0.086
4.24LysSer: 4.24 ± 0.091
4.714LysThr: 4.714 ± 0.115
6.656LysVal: 6.656 ± 0.141
0.489LysTrp: 0.489 ± 0.029
4.147LysTyr: 4.147 ± 0.1
0.0LysXaa: 0.0 ± 0.0
Leu
6.272LeuAla: 6.272 ± 0.115
0.617LeuCys: 0.617 ± 0.034
5.278LeuAsp: 5.278 ± 0.118
7.342LeuGlu: 7.342 ± 0.151
3.999LeuPhe: 3.999 ± 0.118
6.333LeuGly: 6.333 ± 0.124
1.215LeuHis: 1.215 ± 0.051
7.235LeuIle: 7.235 ± 0.158
7.748LeuLys: 7.748 ± 0.15
8.696LeuLeu: 8.696 ± 0.209
1.93LeuMet: 1.93 ± 0.067
5.195LeuAsn: 5.195 ± 0.11
3.189LeuPro: 3.189 ± 0.081
2.271LeuGln: 2.271 ± 0.08
3.092LeuArg: 3.092 ± 0.091
6.405LeuSer: 6.405 ± 0.137
5.404LeuThr: 5.404 ± 0.121
6.547LeuVal: 6.547 ± 0.136
0.499LeuTrp: 0.499 ± 0.032
3.258LeuTyr: 3.258 ± 0.073
0.0LeuXaa: 0.0 ± 0.0
Met
1.436MetAla: 1.436 ± 0.058
0.166MetCys: 0.166 ± 0.015
1.116MetAsp: 1.116 ± 0.041
1.327MetGlu: 1.327 ± 0.054
0.985MetPhe: 0.985 ± 0.045
1.225MetGly: 1.225 ± 0.05
0.314MetHis: 0.314 ± 0.024
1.828MetIle: 1.828 ± 0.067
2.141MetLys: 2.141 ± 0.062
2.096MetLeu: 2.096 ± 0.076
0.501MetMet: 0.501 ± 0.034
1.429MetAsn: 1.429 ± 0.051
0.737MetPro: 0.737 ± 0.038
0.673MetGln: 0.673 ± 0.039
0.838MetArg: 0.838 ± 0.043
1.393MetSer: 1.393 ± 0.053
1.249MetThr: 1.249 ± 0.046
1.296MetVal: 1.296 ± 0.048
0.112MetTrp: 0.112 ± 0.013
0.795MetTyr: 0.795 ± 0.045
0.0MetXaa: 0.0 ± 0.0
Asn
3.083AsnAla: 3.083 ± 0.107
0.352AsnCys: 0.352 ± 0.024
3.26AsnAsp: 3.26 ± 0.126
3.838AsnGlu: 3.838 ± 0.092
2.725AsnPhe: 2.725 ± 0.081
3.739AsnGly: 3.739 ± 0.153
0.887AsnHis: 0.887 ± 0.037
6.009AsnIle: 6.009 ± 0.126
5.754AsnLys: 5.754 ± 0.132
5.314AsnLeu: 5.314 ± 0.103
1.264AsnMet: 1.264 ± 0.049
4.723AsnAsn: 4.723 ± 0.158
2.253AsnPro: 2.253 ± 0.089
1.534AsnGln: 1.534 ± 0.075
2.005AsnArg: 2.005 ± 0.07
3.692AsnSer: 3.692 ± 0.09
3.15AsnThr: 3.15 ± 0.098
3.826AsnVal: 3.826 ± 0.088
0.409AsnTrp: 0.409 ± 0.03
2.978AsnTyr: 2.978 ± 0.107
0.0AsnXaa: 0.0 ± 0.0
Pro
1.872ProAla: 1.872 ± 0.111
0.149ProCys: 0.149 ± 0.017
1.395ProAsp: 1.395 ± 0.056
2.647ProGlu: 2.647 ± 0.102
1.405ProPhe: 1.405 ± 0.054
1.716ProGly: 1.716 ± 0.088
0.42ProHis: 0.42 ± 0.029
2.346ProIle: 2.346 ± 0.069
2.638ProLys: 2.638 ± 0.095
2.383ProLeu: 2.383 ± 0.07
0.584ProMet: 0.584 ± 0.03
1.791ProAsn: 1.791 ± 0.085
0.539ProPro: 0.539 ± 0.029
0.809ProGln: 0.809 ± 0.05
0.787ProArg: 0.787 ± 0.035
1.678ProSer: 1.678 ± 0.055
2.231ProThr: 2.231 ± 0.134
2.366ProVal: 2.366 ± 0.101
0.189ProTrp: 0.189 ± 0.017
1.142ProTyr: 1.142 ± 0.047
0.0ProXaa: 0.0 ± 0.0
Gln
1.842GlnAla: 1.842 ± 0.109
0.112GlnCys: 0.112 ± 0.013
1.483GlnAsp: 1.483 ± 0.05
2.125GlnGlu: 2.125 ± 0.073
1.142GlnPhe: 1.142 ± 0.044
1.74GlnGly: 1.74 ± 0.085
0.44GlnHis: 0.44 ± 0.028
1.991GlnIle: 1.991 ± 0.065
2.249GlnLys: 2.249 ± 0.072
2.494GlnLeu: 2.494 ± 0.076
0.598GlnMet: 0.598 ± 0.039
1.736GlnAsn: 1.736 ± 0.087
0.894GlnPro: 0.894 ± 0.073
0.934GlnGln: 0.934 ± 0.053
1.019GlnArg: 1.019 ± 0.045
1.498GlnSer: 1.498 ± 0.059
1.424GlnThr: 1.424 ± 0.063
1.768GlnVal: 1.768 ± 0.055
0.195GlnTrp: 0.195 ± 0.02
1.104GlnTyr: 1.104 ± 0.048
0.0GlnXaa: 0.0 ± 0.0
Arg
1.923ArgAla: 1.923 ± 0.061
0.199ArgCys: 0.199 ± 0.019
1.933ArgAsp: 1.933 ± 0.084
2.947ArgGlu: 2.947 ± 0.092
1.463ArgPhe: 1.463 ± 0.056
1.984ArgGly: 1.984 ± 0.085
0.482ArgHis: 0.482 ± 0.032
2.779ArgIle: 2.779 ± 0.084
3.196ArgLys: 3.196 ± 0.094
3.09ArgLeu: 3.09 ± 0.088
0.914ArgMet: 0.914 ± 0.044
1.913ArgAsn: 1.913 ± 0.06
0.888ArgPro: 0.888 ± 0.043
1.038ArgGln: 1.038 ± 0.041
1.594ArgArg: 1.594 ± 0.063
1.746ArgSer: 1.746 ± 0.064
1.76ArgThr: 1.76 ± 0.055
2.467ArgVal: 2.467 ± 0.088
0.228ArgTrp: 0.228 ± 0.02
1.451ArgTyr: 1.451 ± 0.054
0.0ArgXaa: 0.0 ± 0.0
Ser
3.153SerAla: 3.153 ± 0.078
0.426SerCys: 0.426 ± 0.029
3.265SerAsp: 3.265 ± 0.084
4.273SerGlu: 4.273 ± 0.093
3.009SerPhe: 3.009 ± 0.087
4.145SerGly: 4.145 ± 0.091
0.945SerHis: 0.945 ± 0.047
5.2SerIle: 5.2 ± 0.124
5.29SerLys: 5.29 ± 0.096
5.837SerLeu: 5.837 ± 0.131
1.238SerMet: 1.238 ± 0.047
3.495SerAsn: 3.495 ± 0.099
1.544SerPro: 1.544 ± 0.06
1.546SerGln: 1.546 ± 0.055
2.02SerArg: 2.02 ± 0.059
4.096SerSer: 4.096 ± 0.098
3.291SerThr: 3.291 ± 0.078
4.126SerVal: 4.126 ± 0.092
0.527SerTrp: 0.527 ± 0.029
2.73SerTyr: 2.73 ± 0.074
0.0SerXaa: 0.0 ± 0.0
Thr
3.685ThrAla: 3.685 ± 0.14
0.343ThrCys: 0.343 ± 0.026
2.908ThrAsp: 2.908 ± 0.111
3.62ThrGlu: 3.62 ± 0.113
2.492ThrPhe: 2.492 ± 0.069
3.739ThrGly: 3.739 ± 0.12
0.856ThrHis: 0.856 ± 0.044
4.74ThrIle: 4.74 ± 0.112
4.752ThrLys: 4.752 ± 0.111
5.214ThrLeu: 5.214 ± 0.109
1.021ThrMet: 1.021 ± 0.046
3.128ThrAsn: 3.128 ± 0.098
2.589ThrPro: 2.589 ± 0.156
1.395ThrGln: 1.395 ± 0.06
1.772ThrArg: 1.772 ± 0.066
3.299ThrSer: 3.299 ± 0.082
3.794ThrThr: 3.794 ± 0.162
4.174ThrVal: 4.174 ± 0.166
0.36ThrTrp: 0.36 ± 0.027
2.237ThrTyr: 2.237 ± 0.086
0.0ThrXaa: 0.0 ± 0.0
Val
4.935ValAla: 4.935 ± 0.105
0.617ValCys: 0.617 ± 0.036
4.478ValAsp: 4.478 ± 0.1
5.764ValGlu: 5.764 ± 0.123
3.216ValPhe: 3.216 ± 0.087
4.56ValGly: 4.56 ± 0.108
0.943ValHis: 0.943 ± 0.046
5.793ValIle: 5.793 ± 0.117
5.924ValLys: 5.924 ± 0.151
6.563ValLeu: 6.563 ± 0.128
1.359ValMet: 1.359 ± 0.053
3.799ValAsn: 3.799 ± 0.116
2.271ValPro: 2.271 ± 0.092
1.745ValGln: 1.745 ± 0.077
2.358ValArg: 2.358 ± 0.078
4.543ValSer: 4.543 ± 0.09
4.183ValThr: 4.183 ± 0.192
5.9ValVal: 5.9 ± 0.166
0.364ValTrp: 0.364 ± 0.025
2.827ValTyr: 2.827 ± 0.085
0.0ValXaa: 0.0 ± 0.0
Trp
0.386TrpAla: 0.386 ± 0.028
0.048TrpCys: 0.048 ± 0.008
0.37TrpAsp: 0.37 ± 0.026
0.404TrpGlu: 0.404 ± 0.03
0.331TrpPhe: 0.331 ± 0.027
0.384TrpGly: 0.384 ± 0.026
0.131TrpHis: 0.131 ± 0.014
0.545TrpIle: 0.545 ± 0.03
0.471TrpLys: 0.471 ± 0.03
0.691TrpLeu: 0.691 ± 0.036
0.163TrpMet: 0.163 ± 0.018
0.379TrpAsn: 0.379 ± 0.033
0.122TrpPro: 0.122 ± 0.013
0.221TrpGln: 0.221 ± 0.02
0.212TrpArg: 0.212 ± 0.016
0.367TrpSer: 0.367 ± 0.028
0.345TrpThr: 0.345 ± 0.026
0.423TrpVal: 0.423 ± 0.039
0.071TrpTrp: 0.071 ± 0.012
0.297TrpTyr: 0.297 ± 0.023
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.171TyrAla: 2.171 ± 0.068
0.294TyrCys: 0.294 ± 0.024
2.474TyrAsp: 2.474 ± 0.073
2.861TyrGlu: 2.861 ± 0.081
2.193TyrPhe: 2.193 ± 0.072
2.455TyrGly: 2.455 ± 0.059
0.622TyrHis: 0.622 ± 0.031
3.416TyrIle: 3.416 ± 0.084
3.6TyrLys: 3.6 ± 0.092
4.164TyrLeu: 4.164 ± 0.104
0.822TyrMet: 0.822 ± 0.046
2.908TyrAsn: 2.908 ± 0.094
1.262TyrPro: 1.262 ± 0.045
1.203TyrGln: 1.203 ± 0.049
1.621TyrArg: 1.621 ± 0.059
2.929TyrSer: 2.929 ± 0.079
2.378TyrThr: 2.378 ± 0.085
2.684TyrVal: 2.684 ± 0.099
0.279TyrTrp: 0.279 ± 0.026
2.151TyrTyr: 2.151 ± 0.078
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1829 proteins (588639 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski