Arthrobacter phage Waltz
Average proteome isoelectric point is 6.43
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 96 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2H4PCD6|A0A2H4PCD6_9CAUD ParB domain-containing protein OS=Arthrobacter phage Waltz OX=2047828 GN=SEA_WALTZ_7 PE=4 SV=1
MM1 pKa = 7.33 SRR3 pKa = 11.84 YY4 pKa = 7.61 EE5 pKa = 4.43 TITDD9 pKa = 3.72 LTVHH13 pKa = 6.47 NLTVLGDD20 pKa = 3.49 VTLPDD25 pKa = 3.52 GTIPEE30 pKa = 4.6 PATVEE35 pKa = 3.87 AVEE38 pKa = 4.5 PVQAPAPLPEE48 pKa = 4.98 KK49 pKa = 10.22 LTMAALGDD57 pKa = 3.93 YY58 pKa = 11.11 LEE60 pKa = 5.02 ALTGALVLAGVLKK73 pKa = 10.51 AYY75 pKa = 8.17 EE76 pKa = 4.37 AAPEE80 pKa = 4.2 APEE83 pKa = 4.33 GAADD87 pKa = 4.02 GAGEE91 pKa = 3.94
Molecular weight: 9.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.081
IPC2_protein 3.91
IPC_protein 3.77
Toseland 3.617
ProMoST 3.872
Dawson 3.745
Bjellqvist 3.986
Wikipedia 3.643
Rodwell 3.617
Grimsley 3.528
Solomon 3.719
Lehninger 3.668
Nozaki 3.872
DTASelect 3.973
Thurlkill 3.656
EMBOSS 3.656
Sillero 3.897
Patrickios 3.02
IPC_peptide 3.719
IPC2_peptide 3.872
IPC2.peptide.svr19 3.834
Protein with the highest isoelectric point:
>tr|A0A2H4PCW1|A0A2H4PCW1_9CAUD Major tail protein OS=Arthrobacter phage Waltz OX=2047828 GN=SEA_WALTZ_62 PE=4 SV=1
MM1 pKa = 6.48 TAKK4 pKa = 9.82 TATRR8 pKa = 11.84 TTAHH12 pKa = 5.15 VVNRR16 pKa = 11.84 TRR18 pKa = 11.84 AEE20 pKa = 3.97 AKK22 pKa = 9.91 RR23 pKa = 11.84 LSGSTALTARR33 pKa = 11.84 MEE35 pKa = 4.3 CAACDD40 pKa = 4.04 SEE42 pKa = 5.94 SPWTPPAFAEE52 pKa = 4.35 AARR55 pKa = 11.84 ADD57 pKa = 4.02 HH58 pKa = 6.16 VCPGRR63 pKa = 11.84 RR64 pKa = 11.84 PGGRR68 pKa = 11.84 RR69 pKa = 11.84 RR70 pKa = 11.84 KK71 pKa = 9.77 QVDD74 pKa = 3.37 SFF76 pKa = 3.87
Molecular weight: 8.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.317
IPC2_protein 9.37
IPC_protein 10.233
Toseland 10.891
ProMoST 10.862
Dawson 10.921
Bjellqvist 10.716
Wikipedia 11.199
Rodwell 10.935
Grimsley 10.935
Solomon 11.199
Lehninger 11.155
Nozaki 10.906
DTASelect 10.701
Thurlkill 10.877
EMBOSS 11.33
Sillero 10.877
Patrickios 10.76
IPC_peptide 11.213
IPC2_peptide 10.175
IPC2.peptide.svr19 9.02
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
96
0
96
17863
30
1030
186.1
20.24
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.935 ± 0.401
0.733 ± 0.11
5.912 ± 0.221
6.018 ± 0.285
2.62 ± 0.202
8.386 ± 0.398
1.959 ± 0.189
4.546 ± 0.213
4.277 ± 0.19
7.541 ± 0.176
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.581 ± 0.124
3.185 ± 0.164
5.805 ± 0.23
3.689 ± 0.217
6.298 ± 0.382
5.285 ± 0.246
7.166 ± 0.235
7.518 ± 0.24
1.864 ± 0.111
2.682 ± 0.196
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here