Amino acid dipepetide frequency for Arthrobacter phage Waltz

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.187AlaAla: 17.187 ± 1.565
0.952AlaCys: 0.952 ± 0.221
7.39AlaAsp: 7.39 ± 0.581
8.398AlaGlu: 8.398 ± 0.744
3.079AlaPhe: 3.079 ± 0.513
10.301AlaGly: 10.301 ± 1.032
1.624AlaHis: 1.624 ± 0.249
6.102AlaIle: 6.102 ± 0.778
5.878AlaLys: 5.878 ± 0.623
9.909AlaLeu: 9.909 ± 0.683
3.247AlaMet: 3.247 ± 0.481
2.183AlaAsn: 2.183 ± 0.41
6.158AlaPro: 6.158 ± 0.741
4.479AlaGln: 4.479 ± 0.519
7.166AlaArg: 7.166 ± 0.866
5.375AlaSer: 5.375 ± 0.626
7.614AlaThr: 7.614 ± 0.71
7.894AlaVal: 7.894 ± 0.76
1.959AlaTrp: 1.959 ± 0.317
2.463AlaTyr: 2.463 ± 0.341
0.0AlaXaa: 0.0 ± 0.0
Cys
0.728CysAla: 0.728 ± 0.216
0.056CysCys: 0.056 ± 0.06
0.224CysAsp: 0.224 ± 0.103
0.336CysGlu: 0.336 ± 0.129
0.168CysPhe: 0.168 ± 0.095
0.952CysGly: 0.952 ± 0.254
0.336CysHis: 0.336 ± 0.131
0.168CysIle: 0.168 ± 0.106
0.28CysLys: 0.28 ± 0.11
0.448CysLeu: 0.448 ± 0.163
0.112CysMet: 0.112 ± 0.08
0.056CysAsn: 0.056 ± 0.049
0.728CysPro: 0.728 ± 0.2
0.448CysGln: 0.448 ± 0.151
0.728CysArg: 0.728 ± 0.235
0.28CysSer: 0.28 ± 0.122
0.392CysThr: 0.392 ± 0.162
0.448CysVal: 0.448 ± 0.17
0.224CysTrp: 0.224 ± 0.102
0.224CysTyr: 0.224 ± 0.116
0.0CysXaa: 0.0 ± 0.0
Asp
7.39AspAla: 7.39 ± 0.846
0.448AspCys: 0.448 ± 0.178
3.023AspAsp: 3.023 ± 0.5
4.591AspGlu: 4.591 ± 0.486
1.624AspPhe: 1.624 ± 0.273
5.822AspGly: 5.822 ± 0.532
1.568AspHis: 1.568 ± 0.306
1.288AspIle: 1.288 ± 0.236
2.127AspLys: 2.127 ± 0.299
5.039AspLeu: 5.039 ± 0.6
2.239AspMet: 2.239 ± 0.366
1.624AspAsn: 1.624 ± 0.299
4.031AspPro: 4.031 ± 0.633
1.288AspGln: 1.288 ± 0.276
3.695AspArg: 3.695 ± 0.563
3.023AspSer: 3.023 ± 0.445
2.687AspThr: 2.687 ± 0.362
4.759AspVal: 4.759 ± 0.603
1.176AspTrp: 1.176 ± 0.224
1.68AspTyr: 1.68 ± 0.289
0.0AspXaa: 0.0 ± 0.0
Glu
8.846GluAla: 8.846 ± 0.84
0.168GluCys: 0.168 ± 0.087
3.975GluAsp: 3.975 ± 0.488
2.631GluGlu: 2.631 ± 0.297
1.903GluPhe: 1.903 ± 0.285
4.255GluGly: 4.255 ± 0.505
1.064GluHis: 1.064 ± 0.207
2.743GluIle: 2.743 ± 0.456
2.239GluLys: 2.239 ± 0.362
4.815GluLeu: 4.815 ± 0.503
1.568GluMet: 1.568 ± 0.361
1.736GluAsn: 1.736 ± 0.281
2.687GluPro: 2.687 ± 0.424
1.736GluGln: 1.736 ± 0.328
4.423GluArg: 4.423 ± 0.544
2.463GluSer: 2.463 ± 0.38
3.639GluThr: 3.639 ± 0.428
5.99GluVal: 5.99 ± 0.585
1.288GluTrp: 1.288 ± 0.237
2.015GluTyr: 2.015 ± 0.382
0.0GluXaa: 0.0 ± 0.0
Phe
2.295PheAla: 2.295 ± 0.383
0.112PheCys: 0.112 ± 0.071
1.903PheAsp: 1.903 ± 0.298
1.176PheGlu: 1.176 ± 0.216
0.392PhePhe: 0.392 ± 0.139
1.68PheGly: 1.68 ± 0.33
0.672PheHis: 0.672 ± 0.217
1.4PheIle: 1.4 ± 0.338
1.176PheLys: 1.176 ± 0.312
2.351PheLeu: 2.351 ± 0.396
1.12PheMet: 1.12 ± 0.299
1.008PheAsn: 1.008 ± 0.315
1.176PhePro: 1.176 ± 0.28
0.448PheGln: 0.448 ± 0.223
1.847PheArg: 1.847 ± 0.284
1.736PheSer: 1.736 ± 0.284
2.911PheThr: 2.911 ± 0.465
1.624PheVal: 1.624 ± 0.355
0.392PheTrp: 0.392 ± 0.116
0.784PheTyr: 0.784 ± 0.262
0.0PheXaa: 0.0 ± 0.0
Gly
8.678GlyAla: 8.678 ± 1.15
0.392GlyCys: 0.392 ± 0.141
4.647GlyAsp: 4.647 ± 0.424
4.423GlyGlu: 4.423 ± 0.527
2.743GlyPhe: 2.743 ± 0.586
8.23GlyGly: 8.23 ± 0.994
1.624GlyHis: 1.624 ± 0.34
3.527GlyIle: 3.527 ± 0.569
3.695GlyLys: 3.695 ± 0.414
5.822GlyLeu: 5.822 ± 0.671
1.903GlyMet: 1.903 ± 0.337
2.687GlyAsn: 2.687 ± 0.415
2.519GlyPro: 2.519 ± 0.375
3.583GlyGln: 3.583 ± 0.656
4.871GlyArg: 4.871 ± 0.469
5.095GlySer: 5.095 ± 0.71
6.158GlyThr: 6.158 ± 0.733
7.222GlyVal: 7.222 ± 0.791
2.855GlyTrp: 2.855 ± 0.404
3.135GlyTyr: 3.135 ± 0.405
0.0GlyXaa: 0.0 ± 0.0
His
1.847HisAla: 1.847 ± 0.291
0.168HisCys: 0.168 ± 0.106
1.344HisAsp: 1.344 ± 0.273
1.288HisGlu: 1.288 ± 0.257
0.504HisPhe: 0.504 ± 0.177
1.736HisGly: 1.736 ± 0.289
0.784HisHis: 0.784 ± 0.235
0.896HisIle: 0.896 ± 0.272
0.504HisLys: 0.504 ± 0.18
1.736HisLeu: 1.736 ± 0.352
0.168HisMet: 0.168 ± 0.098
0.84HisAsn: 0.84 ± 0.25
1.792HisPro: 1.792 ± 0.362
0.504HisGln: 0.504 ± 0.125
1.232HisArg: 1.232 ± 0.276
1.064HisSer: 1.064 ± 0.257
0.784HisThr: 0.784 ± 0.216
1.624HisVal: 1.624 ± 0.315
0.28HisTrp: 0.28 ± 0.117
0.504HisTyr: 0.504 ± 0.158
0.0HisXaa: 0.0 ± 0.0
Ile
4.983IleAla: 4.983 ± 0.55
0.448IleCys: 0.448 ± 0.21
2.239IleAsp: 2.239 ± 0.311
2.015IleGlu: 2.015 ± 0.373
1.456IlePhe: 1.456 ± 0.33
3.583IleGly: 3.583 ± 0.553
0.56IleHis: 0.56 ± 0.158
2.127IleIle: 2.127 ± 0.328
2.519IleLys: 2.519 ± 0.422
3.135IleLeu: 3.135 ± 0.481
0.952IleMet: 0.952 ± 0.168
2.127IleAsn: 2.127 ± 0.379
2.407IlePro: 2.407 ± 0.385
2.071IleGln: 2.071 ± 0.398
3.639IleArg: 3.639 ± 0.442
2.015IleSer: 2.015 ± 0.362
4.367IleThr: 4.367 ± 0.448
3.247IleVal: 3.247 ± 0.487
0.952IleTrp: 0.952 ± 0.246
0.616IleTyr: 0.616 ± 0.183
0.0IleXaa: 0.0 ± 0.0
Lys
5.934LysAla: 5.934 ± 0.645
0.448LysCys: 0.448 ± 0.156
2.687LysAsp: 2.687 ± 0.355
2.183LysGlu: 2.183 ± 0.42
1.344LysPhe: 1.344 ± 0.298
2.463LysGly: 2.463 ± 0.364
0.952LysHis: 0.952 ± 0.266
1.903LysIle: 1.903 ± 0.336
1.288LysLys: 1.288 ± 0.28
3.751LysLeu: 3.751 ± 0.483
1.456LysMet: 1.456 ± 0.296
1.456LysAsn: 1.456 ± 0.302
1.959LysPro: 1.959 ± 0.347
1.456LysGln: 1.456 ± 0.391
2.407LysArg: 2.407 ± 0.313
2.183LysSer: 2.183 ± 0.344
3.359LysThr: 3.359 ± 0.441
3.583LysVal: 3.583 ± 0.409
0.728LysTrp: 0.728 ± 0.231
1.232LysTyr: 1.232 ± 0.265
0.0LysXaa: 0.0 ± 0.0
Leu
7.894LeuAla: 7.894 ± 0.633
0.56LeuCys: 0.56 ± 0.201
4.647LeuAsp: 4.647 ± 0.441
4.871LeuGlu: 4.871 ± 0.609
1.568LeuPhe: 1.568 ± 0.372
6.662LeuGly: 6.662 ± 0.585
1.903LeuHis: 1.903 ± 0.331
3.135LeuIle: 3.135 ± 0.428
4.031LeuLys: 4.031 ± 0.413
4.927LeuLeu: 4.927 ± 0.515
1.624LeuMet: 1.624 ± 0.279
3.359LeuAsn: 3.359 ± 0.404
4.927LeuPro: 4.927 ± 0.611
2.463LeuGln: 2.463 ± 0.464
4.759LeuArg: 4.759 ± 0.51
4.591LeuSer: 4.591 ± 0.504
6.662LeuThr: 6.662 ± 0.646
4.255LeuVal: 4.255 ± 0.44
1.12LeuTrp: 1.12 ± 0.26
1.456LeuTyr: 1.456 ± 0.24
0.0LeuXaa: 0.0 ± 0.0
Met
3.695MetAla: 3.695 ± 0.425
0.168MetCys: 0.168 ± 0.097
1.568MetAsp: 1.568 ± 0.294
1.792MetGlu: 1.792 ± 0.316
0.672MetPhe: 0.672 ± 0.207
1.568MetGly: 1.568 ± 0.375
0.28MetHis: 0.28 ± 0.122
0.56MetIle: 0.56 ± 0.187
1.344MetLys: 1.344 ± 0.268
1.456MetLeu: 1.456 ± 0.273
0.672MetMet: 0.672 ± 0.149
0.896MetAsn: 0.896 ± 0.234
1.959MetPro: 1.959 ± 0.307
0.672MetGln: 0.672 ± 0.188
0.952MetArg: 0.952 ± 0.215
1.903MetSer: 1.903 ± 0.338
3.135MetThr: 3.135 ± 0.44
1.959MetVal: 1.959 ± 0.305
0.392MetTrp: 0.392 ± 0.125
0.168MetTyr: 0.168 ± 0.098
0.0MetXaa: 0.0 ± 0.0
Asn
3.975AsnAla: 3.975 ± 0.716
0.224AsnCys: 0.224 ± 0.111
1.792AsnAsp: 1.792 ± 0.267
1.456AsnGlu: 1.456 ± 0.368
1.288AsnPhe: 1.288 ± 0.236
2.799AsnGly: 2.799 ± 0.467
0.784AsnHis: 0.784 ± 0.2
1.736AsnIle: 1.736 ± 0.312
1.064AsnLys: 1.064 ± 0.258
2.519AsnLeu: 2.519 ± 0.398
0.616AsnMet: 0.616 ± 0.179
0.448AsnAsn: 0.448 ± 0.15
3.079AsnPro: 3.079 ± 0.478
0.952AsnGln: 0.952 ± 0.265
2.295AsnArg: 2.295 ± 0.448
1.792AsnSer: 1.792 ± 0.268
1.736AsnThr: 1.736 ± 0.303
1.847AsnVal: 1.847 ± 0.289
0.672AsnTrp: 0.672 ± 0.243
0.784AsnTyr: 0.784 ± 0.185
0.0AsnXaa: 0.0 ± 0.0
Pro
7.502ProAla: 7.502 ± 0.744
0.504ProCys: 0.504 ± 0.197
3.247ProAsp: 3.247 ± 0.44
5.151ProGlu: 5.151 ± 0.607
0.896ProPhe: 0.896 ± 0.19
5.487ProGly: 5.487 ± 0.542
1.288ProHis: 1.288 ± 0.286
2.183ProIle: 2.183 ± 0.323
2.071ProLys: 2.071 ± 0.364
3.863ProLeu: 3.863 ± 0.435
1.512ProMet: 1.512 ± 0.261
2.183ProAsn: 2.183 ± 0.282
3.135ProPro: 3.135 ± 0.573
2.631ProGln: 2.631 ± 0.66
2.855ProArg: 2.855 ± 0.404
2.967ProSer: 2.967 ± 0.467
2.967ProThr: 2.967 ± 0.421
4.759ProVal: 4.759 ± 0.452
1.064ProTrp: 1.064 ± 0.263
1.792ProTyr: 1.792 ± 0.295
0.0ProXaa: 0.0 ± 0.0
Gln
4.311GlnAla: 4.311 ± 0.542
0.28GlnCys: 0.28 ± 0.149
1.288GlnAsp: 1.288 ± 0.255
1.736GlnGlu: 1.736 ± 0.356
1.344GlnPhe: 1.344 ± 0.263
3.247GlnGly: 3.247 ± 0.532
1.008GlnHis: 1.008 ± 0.239
1.456GlnIle: 1.456 ± 0.275
0.952GlnLys: 0.952 ± 0.209
3.023GlnLeu: 3.023 ± 0.457
0.56GlnMet: 0.56 ± 0.168
0.952GlnAsn: 0.952 ± 0.236
2.799GlnPro: 2.799 ± 0.677
2.183GlnGln: 2.183 ± 0.782
2.743GlnArg: 2.743 ± 0.486
1.903GlnSer: 1.903 ± 0.334
2.183GlnThr: 2.183 ± 0.326
2.575GlnVal: 2.575 ± 0.416
0.616GlnTrp: 0.616 ± 0.161
1.736GlnTyr: 1.736 ± 0.333
0.0GlnXaa: 0.0 ± 0.0
Arg
8.062ArgAla: 8.062 ± 0.892
0.336ArgCys: 0.336 ± 0.154
3.751ArgAsp: 3.751 ± 0.52
4.983ArgGlu: 4.983 ± 0.562
1.568ArgPhe: 1.568 ± 0.302
4.199ArgGly: 4.199 ± 0.543
1.288ArgHis: 1.288 ± 0.305
3.359ArgIle: 3.359 ± 0.435
3.135ArgLys: 3.135 ± 0.429
4.087ArgLeu: 4.087 ± 0.516
1.568ArgMet: 1.568 ± 0.278
1.624ArgAsn: 1.624 ± 0.293
3.807ArgPro: 3.807 ± 0.503
2.743ArgGln: 2.743 ± 0.47
4.535ArgArg: 4.535 ± 0.642
2.911ArgSer: 2.911 ± 0.385
4.087ArgThr: 4.087 ± 0.585
4.087ArgVal: 4.087 ± 0.545
0.84ArgTrp: 0.84 ± 0.234
2.015ArgTyr: 2.015 ± 0.349
0.0ArgXaa: 0.0 ± 0.0
Ser
5.542SerAla: 5.542 ± 0.628
0.392SerCys: 0.392 ± 0.136
2.911SerAsp: 2.911 ± 0.4
2.911SerGlu: 2.911 ± 0.438
1.512SerPhe: 1.512 ± 0.369
5.71SerGly: 5.71 ± 0.726
0.672SerHis: 0.672 ± 0.186
3.303SerIle: 3.303 ± 0.435
2.127SerLys: 2.127 ± 0.377
3.695SerLeu: 3.695 ± 0.438
1.624SerMet: 1.624 ± 0.294
1.512SerAsn: 1.512 ± 0.279
3.135SerPro: 3.135 ± 0.451
2.071SerGln: 2.071 ± 0.341
2.799SerArg: 2.799 ± 0.428
2.911SerSer: 2.911 ± 0.749
4.143SerThr: 4.143 ± 0.624
3.527SerVal: 3.527 ± 0.474
0.896SerTrp: 0.896 ± 0.236
1.456SerTyr: 1.456 ± 0.336
0.0SerXaa: 0.0 ± 0.0
Thr
8.734ThrAla: 8.734 ± 0.671
0.392ThrCys: 0.392 ± 0.159
4.311ThrAsp: 4.311 ± 0.551
3.247ThrGlu: 3.247 ± 0.374
0.952ThrPhe: 0.952 ± 0.208
5.598ThrGly: 5.598 ± 0.89
1.232ThrHis: 1.232 ± 0.276
3.919ThrIle: 3.919 ± 0.533
2.855ThrLys: 2.855 ± 0.427
5.71ThrLeu: 5.71 ± 0.604
1.736ThrMet: 1.736 ± 0.285
2.183ThrAsn: 2.183 ± 0.436
5.934ThrPro: 5.934 ± 0.654
2.575ThrGln: 2.575 ± 0.388
3.863ThrArg: 3.863 ± 0.755
4.367ThrSer: 4.367 ± 0.496
4.255ThrThr: 4.255 ± 0.536
6.83ThrVal: 6.83 ± 0.653
1.176ThrTrp: 1.176 ± 0.246
1.792ThrTyr: 1.792 ± 0.328
0.0ThrXaa: 0.0 ± 0.0
Val
8.062ValAla: 8.062 ± 0.713
0.56ValCys: 0.56 ± 0.2
4.983ValAsp: 4.983 ± 0.612
4.703ValGlu: 4.703 ± 0.643
1.68ValPhe: 1.68 ± 0.403
5.207ValGly: 5.207 ± 0.603
1.232ValHis: 1.232 ± 0.258
3.975ValIle: 3.975 ± 0.46
3.359ValLys: 3.359 ± 0.414
5.431ValLeu: 5.431 ± 0.594
2.127ValMet: 2.127 ± 0.373
2.519ValAsn: 2.519 ± 0.324
3.303ValPro: 3.303 ± 0.354
3.303ValGln: 3.303 ± 0.523
5.151ValArg: 5.151 ± 0.678
3.583ValSer: 3.583 ± 0.448
7.278ValThr: 7.278 ± 0.781
5.598ValVal: 5.598 ± 0.454
1.4ValTrp: 1.4 ± 0.289
1.736ValTyr: 1.736 ± 0.368
0.0ValXaa: 0.0 ± 0.0
Trp
1.456TrpAla: 1.456 ± 0.248
0.28TrpCys: 0.28 ± 0.177
1.4TrpAsp: 1.4 ± 0.245
0.896TrpGlu: 0.896 ± 0.261
0.84TrpPhe: 0.84 ± 0.19
1.68TrpGly: 1.68 ± 0.376
0.28TrpHis: 0.28 ± 0.14
0.448TrpIle: 0.448 ± 0.217
0.784TrpLys: 0.784 ± 0.21
1.232TrpLeu: 1.232 ± 0.232
0.224TrpMet: 0.224 ± 0.1
1.344TrpAsn: 1.344 ± 0.409
0.672TrpPro: 0.672 ± 0.174
0.84TrpGln: 0.84 ± 0.187
1.232TrpArg: 1.232 ± 0.217
1.4TrpSer: 1.4 ± 0.297
1.68TrpThr: 1.68 ± 0.353
1.4TrpVal: 1.4 ± 0.264
0.168TrpTrp: 0.168 ± 0.092
0.392TrpTyr: 0.392 ± 0.135
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.239TyrAla: 2.239 ± 0.425
0.448TyrCys: 0.448 ± 0.146
1.792TyrAsp: 1.792 ± 0.32
1.4TyrGlu: 1.4 ± 0.284
0.672TyrPhe: 0.672 ± 0.189
2.687TyrGly: 2.687 ± 0.392
0.448TyrHis: 0.448 ± 0.145
1.232TyrIle: 1.232 ± 0.293
1.344TyrLys: 1.344 ± 0.278
2.463TyrLeu: 2.463 ± 0.452
0.728TyrMet: 0.728 ± 0.228
1.12TyrAsn: 1.12 ± 0.221
1.847TyrPro: 1.847 ± 0.278
0.448TyrGln: 0.448 ± 0.174
1.792TyrArg: 1.792 ± 0.305
1.288TyrSer: 1.288 ± 0.23
1.624TyrThr: 1.624 ± 0.431
1.959TyrVal: 1.959 ± 0.357
0.448TyrTrp: 0.448 ± 0.206
0.84TyrTyr: 0.84 ± 0.226
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 96 proteins (17863 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski