Arthrobacter phage Seahorse

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 100 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G3M4X4|A0A3G3M4X4_9CAUD Uncharacterized protein OS=Arthrobacter phage Seahorse OX=2419611 GN=60 PE=4 SV=1
MM1 pKa = 6.49TQRR4 pKa = 11.84IVSVGDD10 pKa = 4.01DD11 pKa = 3.53FALPPDD17 pKa = 3.93VNVLDD22 pKa = 4.06EE23 pKa = 4.17QLPARR28 pKa = 11.84LQDD31 pKa = 3.33TALNATYY38 pKa = 10.69AGAFPDD44 pKa = 3.98SQGITYY50 pKa = 10.39NADD53 pKa = 2.95GSVATVTEE61 pKa = 4.01NGITTSYY68 pKa = 10.0TYY70 pKa = 11.02NADD73 pKa = 3.15GTVASDD79 pKa = 2.86SRR81 pKa = 11.84TVGGVTTVRR90 pKa = 11.84NYY92 pKa = 10.72TYY94 pKa = 10.93TNGNLTSITKK104 pKa = 9.48EE105 pKa = 3.73AA106 pKa = 3.91

Molecular weight: 11.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G3M6L7|A0A3G3M6L7_9CAUD Uncharacterized protein OS=Arthrobacter phage Seahorse OX=2419611 GN=99 PE=4 SV=1
MM1 pKa = 7.66SKK3 pKa = 10.76GDD5 pKa = 3.33RR6 pKa = 11.84DD7 pKa = 3.63GRR9 pKa = 11.84GFRR12 pKa = 11.84HH13 pKa = 6.15HH14 pKa = 6.98KK15 pKa = 9.9KK16 pKa = 10.46CPSLNCDD23 pKa = 2.64RR24 pKa = 11.84CVNGRR29 pKa = 11.84AKK31 pKa = 10.31RR32 pKa = 11.84PYY34 pKa = 8.39RR35 pKa = 11.84QKK37 pKa = 10.13QRR39 pKa = 11.84RR40 pKa = 11.84EE41 pKa = 3.83AKK43 pKa = 9.32HH44 pKa = 5.74ALRR47 pKa = 11.84TEE49 pKa = 3.99PAEE52 pKa = 4.01

Molecular weight: 6.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

100

0

100

17816

38

1350

178.2

19.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.989 ± 0.609

0.965 ± 0.135

6.618 ± 0.219

5.787 ± 0.285

2.93 ± 0.142

8.167 ± 0.366

2.341 ± 0.197

4.541 ± 0.15

4.558 ± 0.239

7.791 ± 0.267

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.161 ± 0.125

3.267 ± 0.187

5.456 ± 0.305

3.418 ± 0.181

6.511 ± 0.322

5.607 ± 0.221

6.567 ± 0.272

6.741 ± 0.222

1.959 ± 0.203

2.627 ± 0.151
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski