Amino acid dipepetide frequency for Arthrobacter phage Seahorse

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.569AlaAla: 17.569 ± 1.833
0.73AlaCys: 0.73 ± 0.2
9.037AlaAsp: 9.037 ± 0.692
7.69AlaGlu: 7.69 ± 0.694
2.807AlaPhe: 2.807 ± 0.341
10.441AlaGly: 10.441 ± 1.145
2.526AlaHis: 2.526 ± 0.521
5.052AlaIle: 5.052 ± 0.504
6.399AlaLys: 6.399 ± 0.625
9.543AlaLeu: 9.543 ± 0.691
3.536AlaMet: 3.536 ± 0.504
3.705AlaAsn: 3.705 ± 0.478
5.276AlaPro: 5.276 ± 0.592
4.827AlaGln: 4.827 ± 0.546
6.904AlaArg: 6.904 ± 0.76
5.501AlaSer: 5.501 ± 0.565
7.073AlaThr: 7.073 ± 0.842
6.792AlaVal: 6.792 ± 0.705
2.245AlaTrp: 2.245 ± 0.37
2.245AlaTyr: 2.245 ± 0.382
0.0AlaXaa: 0.0 ± 0.0
Cys
0.898CysAla: 0.898 ± 0.311
0.112CysCys: 0.112 ± 0.074
1.01CysAsp: 1.01 ± 0.267
0.393CysGlu: 0.393 ± 0.143
0.225CysPhe: 0.225 ± 0.104
1.123CysGly: 1.123 ± 0.306
0.225CysHis: 0.225 ± 0.124
0.281CysIle: 0.281 ± 0.137
0.674CysLys: 0.674 ± 0.185
0.393CysLeu: 0.393 ± 0.148
0.056CysMet: 0.056 ± 0.066
0.225CysAsn: 0.225 ± 0.106
1.123CysPro: 1.123 ± 0.304
0.112CysGln: 0.112 ± 0.072
0.617CysArg: 0.617 ± 0.18
0.617CysSer: 0.617 ± 0.21
0.954CysThr: 0.954 ± 0.235
0.393CysVal: 0.393 ± 0.162
0.112CysTrp: 0.112 ± 0.088
0.112CysTyr: 0.112 ± 0.068
0.0CysXaa: 0.0 ± 0.0
Asp
8.308AspAla: 8.308 ± 0.8
0.617AspCys: 0.617 ± 0.224
3.761AspAsp: 3.761 ± 0.434
4.603AspGlu: 4.603 ± 0.568
2.133AspPhe: 2.133 ± 0.324
6.904AspGly: 6.904 ± 0.818
1.347AspHis: 1.347 ± 0.262
3.256AspIle: 3.256 ± 0.414
2.582AspLys: 2.582 ± 0.393
4.996AspLeu: 4.996 ± 0.563
1.235AspMet: 1.235 ± 0.238
1.572AspAsn: 1.572 ± 0.333
3.985AspPro: 3.985 ± 0.497
1.852AspGln: 1.852 ± 0.292
3.649AspArg: 3.649 ± 0.508
3.312AspSer: 3.312 ± 0.523
2.919AspThr: 2.919 ± 0.429
5.333AspVal: 5.333 ± 0.679
1.572AspTrp: 1.572 ± 0.317
2.245AspTyr: 2.245 ± 0.366
0.0AspXaa: 0.0 ± 0.0
Glu
7.915GluAla: 7.915 ± 0.701
0.449GluCys: 0.449 ± 0.148
3.2GluAsp: 3.2 ± 0.483
2.189GluGlu: 2.189 ± 0.363
1.516GluPhe: 1.516 ± 0.228
4.547GluGly: 4.547 ± 0.468
1.684GluHis: 1.684 ± 0.344
2.975GluIle: 2.975 ± 0.436
2.414GluLys: 2.414 ± 0.362
4.21GluLeu: 4.21 ± 0.559
1.235GluMet: 1.235 ± 0.217
1.796GluAsn: 1.796 ± 0.272
2.863GluPro: 2.863 ± 0.466
3.143GluGln: 3.143 ± 0.412
4.322GluArg: 4.322 ± 0.548
2.919GluSer: 2.919 ± 0.407
2.582GluThr: 2.582 ± 0.294
4.322GluVal: 4.322 ± 0.456
2.021GluTrp: 2.021 ± 0.422
1.516GluTyr: 1.516 ± 0.281
0.0GluXaa: 0.0 ± 0.0
Phe
3.031PheAla: 3.031 ± 0.424
0.393PheCys: 0.393 ± 0.166
2.638PheAsp: 2.638 ± 0.356
1.235PheGlu: 1.235 ± 0.294
0.954PhePhe: 0.954 ± 0.264
2.526PheGly: 2.526 ± 0.395
1.01PheHis: 1.01 ± 0.257
0.898PheIle: 0.898 ± 0.237
1.291PheLys: 1.291 ± 0.242
1.572PheLeu: 1.572 ± 0.385
0.786PheMet: 0.786 ± 0.247
1.123PheAsn: 1.123 ± 0.223
1.235PhePro: 1.235 ± 0.282
1.067PheGln: 1.067 ± 0.19
2.189PheArg: 2.189 ± 0.33
1.796PheSer: 1.796 ± 0.309
2.077PheThr: 2.077 ± 0.363
1.852PheVal: 1.852 ± 0.382
0.73PheTrp: 0.73 ± 0.184
0.898PheTyr: 0.898 ± 0.199
0.0PheXaa: 0.0 ± 0.0
Gly
7.185GlyAla: 7.185 ± 0.811
0.674GlyCys: 0.674 ± 0.199
4.603GlyAsp: 4.603 ± 0.458
4.603GlyGlu: 4.603 ± 0.488
2.975GlyPhe: 2.975 ± 0.37
7.073GlyGly: 7.073 ± 0.839
1.74GlyHis: 1.74 ± 0.29
3.48GlyIle: 3.48 ± 0.6
5.108GlyLys: 5.108 ± 0.641
6.792GlyLeu: 6.792 ± 0.871
2.021GlyMet: 2.021 ± 0.26
3.368GlyAsn: 3.368 ± 0.37
3.536GlyPro: 3.536 ± 0.589
2.75GlyGln: 2.75 ± 0.447
5.108GlyArg: 5.108 ± 0.599
4.827GlySer: 4.827 ± 0.571
5.782GlyThr: 5.782 ± 0.785
4.884GlyVal: 4.884 ± 0.64
2.47GlyTrp: 2.47 ± 0.393
2.694GlyTyr: 2.694 ± 0.387
0.0GlyXaa: 0.0 ± 0.0
His
2.638HisAla: 2.638 ± 0.371
0.505HisCys: 0.505 ± 0.184
1.459HisAsp: 1.459 ± 0.333
1.684HisGlu: 1.684 ± 0.329
0.786HisPhe: 0.786 ± 0.247
1.852HisGly: 1.852 ± 0.345
0.73HisHis: 0.73 ± 0.238
1.235HisIle: 1.235 ± 0.276
0.842HisLys: 0.842 ± 0.245
1.852HisLeu: 1.852 ± 0.344
0.393HisMet: 0.393 ± 0.148
0.617HisAsn: 0.617 ± 0.173
1.516HisPro: 1.516 ± 0.326
0.674HisGln: 0.674 ± 0.203
1.291HisArg: 1.291 ± 0.269
1.01HisSer: 1.01 ± 0.247
1.347HisThr: 1.347 ± 0.211
1.572HisVal: 1.572 ± 0.255
0.449HisTrp: 0.449 ± 0.173
0.954HisTyr: 0.954 ± 0.226
0.0HisXaa: 0.0 ± 0.0
Ile
4.378IleAla: 4.378 ± 0.572
0.393IleCys: 0.393 ± 0.167
3.761IleAsp: 3.761 ± 0.475
3.424IleGlu: 3.424 ± 0.372
1.067IlePhe: 1.067 ± 0.266
2.919IleGly: 2.919 ± 0.383
1.179IleHis: 1.179 ± 0.269
1.796IleIle: 1.796 ± 0.326
1.572IleLys: 1.572 ± 0.267
2.863IleLeu: 2.863 ± 0.453
0.393IleMet: 0.393 ± 0.142
1.347IleAsn: 1.347 ± 0.258
2.807IlePro: 2.807 ± 0.415
1.572IleGln: 1.572 ± 0.323
3.536IleArg: 3.536 ± 0.475
2.863IleSer: 2.863 ± 0.437
4.771IleThr: 4.771 ± 0.501
2.863IleVal: 2.863 ± 0.415
0.281IleTrp: 0.281 ± 0.141
1.628IleTyr: 1.628 ± 0.329
0.0IleXaa: 0.0 ± 0.0
Lys
6.455LysAla: 6.455 ± 0.75
0.449LysCys: 0.449 ± 0.178
2.863LysAsp: 2.863 ± 0.431
1.965LysGlu: 1.965 ± 0.372
1.909LysPhe: 1.909 ± 0.315
2.526LysGly: 2.526 ± 0.459
1.235LysHis: 1.235 ± 0.257
1.965LysIle: 1.965 ± 0.371
1.628LysLys: 1.628 ± 0.331
3.592LysLeu: 3.592 ± 0.432
1.235LysMet: 1.235 ± 0.202
1.516LysAsn: 1.516 ± 0.271
2.919LysPro: 2.919 ± 0.474
1.796LysGln: 1.796 ± 0.294
3.705LysArg: 3.705 ± 0.525
2.245LysSer: 2.245 ± 0.428
3.087LysThr: 3.087 ± 0.356
2.975LysVal: 2.975 ± 0.374
0.786LysTrp: 0.786 ± 0.241
0.73LysTyr: 0.73 ± 0.162
0.0LysXaa: 0.0 ± 0.0
Leu
10.385LeuAla: 10.385 ± 1.021
0.898LeuCys: 0.898 ± 0.28
4.827LeuAsp: 4.827 ± 0.518
4.266LeuGlu: 4.266 ± 0.54
2.526LeuPhe: 2.526 ± 0.496
6.455LeuGly: 6.455 ± 0.649
1.965LeuHis: 1.965 ± 0.347
4.154LeuIle: 4.154 ± 0.472
3.424LeuLys: 3.424 ± 0.434
6.006LeuLeu: 6.006 ± 0.628
1.347LeuMet: 1.347 ± 0.298
2.919LeuAsn: 2.919 ± 0.473
4.434LeuPro: 4.434 ± 0.478
1.796LeuGln: 1.796 ± 0.315
5.108LeuArg: 5.108 ± 0.544
4.491LeuSer: 4.491 ± 0.459
5.838LeuThr: 5.838 ± 0.764
4.659LeuVal: 4.659 ± 0.508
1.01LeuTrp: 1.01 ± 0.269
1.403LeuTyr: 1.403 ± 0.248
0.0LeuXaa: 0.0 ± 0.0
Met
2.414MetAla: 2.414 ± 0.453
0.112MetCys: 0.112 ± 0.094
1.235MetAsp: 1.235 ± 0.245
0.786MetGlu: 0.786 ± 0.195
0.674MetPhe: 0.674 ± 0.212
0.898MetGly: 0.898 ± 0.25
0.73MetHis: 0.73 ± 0.197
0.898MetIle: 0.898 ± 0.232
0.674MetLys: 0.674 ± 0.142
1.684MetLeu: 1.684 ± 0.284
0.337MetMet: 0.337 ± 0.139
1.123MetAsn: 1.123 ± 0.26
1.74MetPro: 1.74 ± 0.386
0.449MetGln: 0.449 ± 0.14
1.403MetArg: 1.403 ± 0.261
2.301MetSer: 2.301 ± 0.3
2.077MetThr: 2.077 ± 0.403
1.179MetVal: 1.179 ± 0.192
0.561MetTrp: 0.561 ± 0.162
0.337MetTyr: 0.337 ± 0.132
0.0MetXaa: 0.0 ± 0.0
Asn
4.547AsnAla: 4.547 ± 0.549
0.393AsnCys: 0.393 ± 0.15
1.965AsnAsp: 1.965 ± 0.426
2.077AsnGlu: 2.077 ± 0.416
0.281AsnPhe: 0.281 ± 0.121
3.592AsnGly: 3.592 ± 0.453
1.459AsnHis: 1.459 ± 0.283
1.123AsnIle: 1.123 ± 0.259
1.403AsnLys: 1.403 ± 0.273
2.694AsnLeu: 2.694 ± 0.58
0.393AsnMet: 0.393 ± 0.134
1.067AsnAsn: 1.067 ± 0.221
2.077AsnPro: 2.077 ± 0.355
1.123AsnGln: 1.123 ± 0.249
2.301AsnArg: 2.301 ± 0.365
1.179AsnSer: 1.179 ± 0.228
1.628AsnThr: 1.628 ± 0.326
2.133AsnVal: 2.133 ± 0.271
0.561AsnTrp: 0.561 ± 0.19
0.674AsnTyr: 0.674 ± 0.205
0.0AsnXaa: 0.0 ± 0.0
Pro
6.455ProAla: 6.455 ± 0.698
0.561ProCys: 0.561 ± 0.198
4.21ProAsp: 4.21 ± 0.598
3.649ProGlu: 3.649 ± 0.531
1.796ProPhe: 1.796 ± 0.248
4.715ProGly: 4.715 ± 0.488
0.842ProHis: 0.842 ± 0.189
2.189ProIle: 2.189 ± 0.351
2.526ProLys: 2.526 ± 0.269
4.266ProLeu: 4.266 ± 0.643
1.403ProMet: 1.403 ± 0.29
2.021ProAsn: 2.021 ± 0.305
2.694ProPro: 2.694 ± 0.531
1.628ProGln: 1.628 ± 0.325
3.143ProArg: 3.143 ± 0.464
3.2ProSer: 3.2 ± 0.393
2.975ProThr: 2.975 ± 0.46
4.378ProVal: 4.378 ± 0.613
0.842ProTrp: 0.842 ± 0.278
1.067ProTyr: 1.067 ± 0.219
0.0ProXaa: 0.0 ± 0.0
Gln
4.491GlnAla: 4.491 ± 0.638
0.393GlnCys: 0.393 ± 0.145
2.301GlnAsp: 2.301 ± 0.393
1.572GlnGlu: 1.572 ± 0.298
0.617GlnPhe: 0.617 ± 0.143
2.807GlnGly: 2.807 ± 0.565
0.786GlnHis: 0.786 ± 0.177
1.291GlnIle: 1.291 ± 0.268
1.572GlnLys: 1.572 ± 0.297
2.807GlnLeu: 2.807 ± 0.463
0.842GlnMet: 0.842 ± 0.208
0.842GlnAsn: 0.842 ± 0.206
1.628GlnPro: 1.628 ± 0.353
1.628GlnGln: 1.628 ± 0.29
2.245GlnArg: 2.245 ± 0.404
2.301GlnSer: 2.301 ± 0.362
2.638GlnThr: 2.638 ± 0.355
2.189GlnVal: 2.189 ± 0.416
0.505GlnTrp: 0.505 ± 0.188
0.73GlnTyr: 0.73 ± 0.208
0.0GlnXaa: 0.0 ± 0.0
Arg
6.175ArgAla: 6.175 ± 0.523
0.674ArgCys: 0.674 ± 0.231
4.491ArgAsp: 4.491 ± 0.422
3.312ArgGlu: 3.312 ± 0.395
2.077ArgPhe: 2.077 ± 0.341
4.491ArgGly: 4.491 ± 0.419
1.459ArgHis: 1.459 ± 0.466
4.042ArgIle: 4.042 ± 0.477
2.975ArgLys: 2.975 ± 0.294
5.726ArgLeu: 5.726 ± 0.537
1.852ArgMet: 1.852 ± 0.358
1.965ArgAsn: 1.965 ± 0.357
3.761ArgPro: 3.761 ± 0.441
1.628ArgGln: 1.628 ± 0.293
5.052ArgArg: 5.052 ± 0.569
3.368ArgSer: 3.368 ± 0.385
4.098ArgThr: 4.098 ± 0.425
3.649ArgVal: 3.649 ± 0.514
1.74ArgTrp: 1.74 ± 0.327
2.582ArgTyr: 2.582 ± 0.401
0.0ArgXaa: 0.0 ± 0.0
Ser
5.669SerAla: 5.669 ± 0.443
0.281SerCys: 0.281 ± 0.131
3.873SerAsp: 3.873 ± 0.41
3.985SerGlu: 3.985 ± 0.451
1.965SerPhe: 1.965 ± 0.364
4.827SerGly: 4.827 ± 0.7
0.786SerHis: 0.786 ± 0.252
2.975SerIle: 2.975 ± 0.439
2.582SerLys: 2.582 ± 0.317
5.22SerLeu: 5.22 ± 0.731
1.459SerMet: 1.459 ± 0.287
1.235SerAsn: 1.235 ± 0.249
2.863SerPro: 2.863 ± 0.429
1.965SerGln: 1.965 ± 0.304
3.592SerArg: 3.592 ± 0.483
3.256SerSer: 3.256 ± 0.619
3.761SerThr: 3.761 ± 0.458
3.649SerVal: 3.649 ± 0.413
0.898SerTrp: 0.898 ± 0.227
1.235SerTyr: 1.235 ± 0.292
0.0SerXaa: 0.0 ± 0.0
Thr
8.42ThrAla: 8.42 ± 0.86
0.617ThrCys: 0.617 ± 0.208
3.592ThrAsp: 3.592 ± 0.356
3.649ThrGlu: 3.649 ± 0.438
2.021ThrPhe: 2.021 ± 0.336
5.95ThrGly: 5.95 ± 0.634
1.01ThrHis: 1.01 ± 0.206
3.312ThrIle: 3.312 ± 0.432
2.807ThrLys: 2.807 ± 0.372
4.603ThrLeu: 4.603 ± 0.613
1.179ThrMet: 1.179 ± 0.214
2.47ThrAsn: 2.47 ± 0.365
3.873ThrPro: 3.873 ± 0.433
1.628ThrGln: 1.628 ± 0.249
3.256ThrArg: 3.256 ± 0.406
3.705ThrSer: 3.705 ± 0.623
3.929ThrThr: 3.929 ± 0.431
6.343ThrVal: 6.343 ± 0.65
0.898ThrTrp: 0.898 ± 0.269
2.414ThrTyr: 2.414 ± 0.45
0.0ThrXaa: 0.0 ± 0.0
Val
8.251ValAla: 8.251 ± 0.631
0.674ValCys: 0.674 ± 0.185
4.715ValAsp: 4.715 ± 0.569
3.48ValGlu: 3.48 ± 0.498
1.965ValPhe: 1.965 ± 0.237
4.322ValGly: 4.322 ± 0.578
1.74ValHis: 1.74 ± 0.328
2.807ValIle: 2.807 ± 0.414
3.2ValLys: 3.2 ± 0.462
5.445ValLeu: 5.445 ± 0.546
1.291ValMet: 1.291 ± 0.204
2.021ValAsn: 2.021 ± 0.308
4.042ValPro: 4.042 ± 0.576
2.414ValGln: 2.414 ± 0.312
3.817ValArg: 3.817 ± 0.488
3.985ValSer: 3.985 ± 0.36
5.276ValThr: 5.276 ± 0.507
5.276ValVal: 5.276 ± 0.698
1.01ValTrp: 1.01 ± 0.244
1.684ValTyr: 1.684 ± 0.31
0.0ValXaa: 0.0 ± 0.0
Trp
2.694TrpAla: 2.694 ± 0.338
0.393TrpCys: 0.393 ± 0.143
1.235TrpAsp: 1.235 ± 0.313
1.403TrpGlu: 1.403 ± 0.335
0.225TrpPhe: 0.225 ± 0.111
1.235TrpGly: 1.235 ± 0.267
0.617TrpHis: 0.617 ± 0.206
0.674TrpIle: 0.674 ± 0.162
0.954TrpLys: 0.954 ± 0.287
1.572TrpLeu: 1.572 ± 0.289
0.168TrpMet: 0.168 ± 0.096
0.505TrpAsn: 0.505 ± 0.206
0.954TrpPro: 0.954 ± 0.241
0.842TrpGln: 0.842 ± 0.193
1.516TrpArg: 1.516 ± 0.276
1.572TrpSer: 1.572 ± 0.264
0.842TrpThr: 0.842 ± 0.198
1.067TrpVal: 1.067 ± 0.195
0.617TrpTrp: 0.617 ± 0.22
0.505TrpTyr: 0.505 ± 0.168
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.021TyrAla: 2.021 ± 0.345
0.337TyrCys: 0.337 ± 0.188
1.403TyrAsp: 1.403 ± 0.233
1.909TyrGlu: 1.909 ± 0.312
0.786TyrPhe: 0.786 ± 0.196
2.47TyrGly: 2.47 ± 0.428
0.337TyrHis: 0.337 ± 0.151
1.01TyrIle: 1.01 ± 0.204
0.954TyrLys: 0.954 ± 0.209
2.077TyrLeu: 2.077 ± 0.338
0.393TyrMet: 0.393 ± 0.126
1.235TyrAsn: 1.235 ± 0.257
1.235TyrPro: 1.235 ± 0.303
1.291TyrGln: 1.291 ± 0.273
2.358TyrArg: 2.358 ± 0.251
1.628TyrSer: 1.628 ± 0.354
2.021TyrThr: 2.021 ± 0.346
1.909TyrVal: 1.909 ± 0.276
0.281TyrTrp: 0.281 ± 0.153
0.617TyrTyr: 0.617 ± 0.174
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 100 proteins (17816 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski