Sulfitobacter guttiformis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Sulfitobacter

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3751 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A420DK11|A0A420DK11_9RHOB Histidinol-phosphatase OS=Sulfitobacter guttiformis OX=74349 GN=C8N30_3695 PE=3 SV=1
MM1 pKa = 7.88RR2 pKa = 11.84NQIISLALIAGLAACSGGNPFDD24 pKa = 4.46DD25 pKa = 3.87TAEE28 pKa = 4.12TDD30 pKa = 3.4GGGTAEE36 pKa = 4.2EE37 pKa = 4.47TGGIPAVLAQNLQSFSYY54 pKa = 10.56NPAAGTLTVTGVPTDD69 pKa = 3.9DD70 pKa = 3.86GVLDD74 pKa = 3.59GVYY77 pKa = 10.17RR78 pKa = 11.84RR79 pKa = 11.84RR80 pKa = 11.84PALDD84 pKa = 3.02RR85 pKa = 11.84AGYY88 pKa = 8.63EE89 pKa = 4.13AYY91 pKa = 8.65TAQDD95 pKa = 3.27GSLDD99 pKa = 3.67RR100 pKa = 11.84HH101 pKa = 4.47ITAYY105 pKa = 10.49VRR107 pKa = 11.84DD108 pKa = 3.75IRR110 pKa = 11.84GTRR113 pKa = 11.84AITIVSGSQFEE124 pKa = 4.26EE125 pKa = 4.55VVAGNAYY132 pKa = 11.02SNTTYY137 pKa = 10.67SAPAATQDD145 pKa = 3.58GGLVTYY151 pKa = 10.59AGTYY155 pKa = 10.06VGLLNGGGSNEE166 pKa = 4.64DD167 pKa = 4.14LLDD170 pKa = 3.78VTPGTSPSVLSAQAAEE186 pKa = 4.17VRR188 pKa = 11.84GRR190 pKa = 11.84ALINGDD196 pKa = 3.64FADD199 pKa = 3.98STVTGAIFEE208 pKa = 4.27RR209 pKa = 11.84VIVDD213 pKa = 3.05VDD215 pKa = 4.16SNSNQDD221 pKa = 3.47VNSADD226 pKa = 3.93PFPLNDD232 pKa = 3.26VALDD236 pKa = 3.73LTDD239 pKa = 3.38IAEE242 pKa = 4.82DD243 pKa = 3.92GTFQGVATFNDD254 pKa = 4.04SAIGAYY260 pKa = 10.07GGIFGGTGATEE271 pKa = 3.93VAGVVVLNNHH281 pKa = 4.89ITGLQGVIEE290 pKa = 4.39NGAFVLSQCGQADD303 pKa = 3.61QDD305 pKa = 4.89PICTQPFEE313 pKa = 4.16

Molecular weight: 32.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A420DQD2|A0A420DQD2_9RHOB EAL domain-containing protein (Putative c-di-GMP-specific phosphodiesterase class I) OS=Sulfitobacter guttiformis OX=74349 GN=C8N30_0931 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.81IINARR34 pKa = 11.84RR35 pKa = 11.84AQGRR39 pKa = 11.84KK40 pKa = 8.52EE41 pKa = 3.76LSAA44 pKa = 4.93

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3751

0

3751

1167314

25

6195

311.2

33.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.004 ± 0.054

0.915 ± 0.013

5.983 ± 0.043

5.578 ± 0.035

3.783 ± 0.03

8.572 ± 0.034

2.039 ± 0.026

5.584 ± 0.032

3.464 ± 0.028

9.817 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.989 ± 0.027

2.874 ± 0.026

4.812 ± 0.029

3.325 ± 0.024

6.127 ± 0.035

5.413 ± 0.027

5.801 ± 0.049

7.35 ± 0.036

1.324 ± 0.017

2.247 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski