Spirosoma sordidisoli

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Cytophagaceae; Spirosoma

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6027 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A4Q2UGD5|A0A4Q2UGD5_9BACT AAA family ATPase OS=Spirosoma sordidisoli OX=2502893 GN=EQG79_22305 PE=4 SV=1
MM1 pKa = 8.09DD2 pKa = 5.87LFTFKK7 pKa = 10.12TYY9 pKa = 10.92SKK11 pKa = 10.31LQVRR15 pKa = 11.84TLLRR19 pKa = 11.84ALCALDD25 pKa = 3.53WPGLKK30 pKa = 9.86PAVIRR35 pKa = 11.84FVVLCAFMLLNMGWPALAEE54 pKa = 3.99RR55 pKa = 11.84KK56 pKa = 8.93PAVLTAVRR64 pKa = 11.84SYY66 pKa = 10.09TGTVAPEE73 pKa = 4.06PVGHH77 pKa = 7.12KK78 pKa = 10.55SPATASFMRR87 pKa = 11.84ARR89 pKa = 11.84SALALDD95 pKa = 3.77SDD97 pKa = 4.56GDD99 pKa = 4.06NVDD102 pKa = 4.97DD103 pKa = 6.46SVDD106 pKa = 4.32LDD108 pKa = 4.48DD109 pKa = 6.83DD110 pKa = 4.16NDD112 pKa = 5.13GILDD116 pKa = 4.0TAEE119 pKa = 4.27MTCQLVTNGDD129 pKa = 3.85FASTTGGWSFTGNVGITGGTLFFNGTNTTPDD160 pKa = 4.02GIATQDD166 pKa = 2.74IQTRR170 pKa = 11.84AGKK173 pKa = 10.03RR174 pKa = 11.84AMLTFDD180 pKa = 4.29FVAAGAGTGTIGVTADD196 pKa = 3.46VLDD199 pKa = 4.04VSTGTVLGTTSLTSTAGTPSSNISIPFTATGSSIRR234 pKa = 11.84IRR236 pKa = 11.84FTDD239 pKa = 3.46ISQATLSIDD248 pKa = 3.54VAIDD252 pKa = 3.42NVSILYY258 pKa = 10.45CDD260 pKa = 3.8TDD262 pKa = 3.49NDD264 pKa = 5.27GIPDD268 pKa = 4.07SLDD271 pKa = 3.5LDD273 pKa = 4.06SDD275 pKa = 4.14GDD277 pKa = 3.92GCADD281 pKa = 4.36AIEE284 pKa = 4.89GGGPFRR290 pKa = 11.84STNLVNSTMAGGNSGVGYY308 pKa = 10.4NGISTSPVTQNLGISVGATGIPTVAGTGQTVGFSQFRR345 pKa = 11.84SLNGCIDD352 pKa = 3.9SEE354 pKa = 4.82DD355 pKa = 3.6DD356 pKa = 3.44TVADD360 pKa = 5.36VEE362 pKa = 5.91DD363 pKa = 5.49LDD365 pKa = 5.6DD366 pKa = 5.93DD367 pKa = 4.41NDD369 pKa = 5.09GILDD373 pKa = 3.98TDD375 pKa = 3.89EE376 pKa = 4.86MNCQLIEE383 pKa = 4.32NGDD386 pKa = 3.91FASTTAGWSFTNNVGITGGFLFFNGSDD413 pKa = 3.47VTPNGIATQDD423 pKa = 2.91IQTRR427 pKa = 11.84AGQRR431 pKa = 11.84AMLTFDD437 pKa = 4.26FVAAGAGAGLVGVRR451 pKa = 11.84IDD453 pKa = 3.7VLDD456 pKa = 3.75VSTGTVLATTSLLRR470 pKa = 11.84TANTPTVTVPLAFTATGTSTRR491 pKa = 11.84IRR493 pKa = 11.84FTDD496 pKa = 3.44ISQATLVIDD505 pKa = 3.78ARR507 pKa = 11.84IDD509 pKa = 3.56NVSILYY515 pKa = 10.45CDD517 pKa = 3.64TDD519 pKa = 3.33NDD521 pKa = 4.92GIANSLDD528 pKa = 3.76LDD530 pKa = 4.15SDD532 pKa = 4.21GDD534 pKa = 3.98GCPDD538 pKa = 3.36AVEE541 pKa = 4.96GGASFKK547 pKa = 11.11VADD550 pKa = 4.77LVNSILPGGNSGTGYY565 pKa = 10.21IGMSTSPVTQNLGTTVGTNGVPTVAGTGQTVGVSQLAGLNGCTDD609 pKa = 3.56SDD611 pKa = 4.27SDD613 pKa = 3.92TVADD617 pKa = 5.83IEE619 pKa = 5.93DD620 pKa = 4.75LDD622 pKa = 4.96DD623 pKa = 5.86DD624 pKa = 4.73NDD626 pKa = 5.14GILDD630 pKa = 3.9TAEE633 pKa = 4.18NTCMLPLTGTPHH645 pKa = 7.65LSWDD649 pKa = 3.67NNGIPLPGTLQINSLSPVVLEE670 pKa = 4.38TTSITTGPGITLGSSGDD687 pKa = 3.16SWVISGATATNLAGAVAGNDD707 pKa = 3.3YY708 pKa = 9.96MQFTMATSATATVDD722 pKa = 3.39YY723 pKa = 10.55TLDD726 pKa = 3.03QWASFQSTAPFQKK739 pKa = 9.6IGIRR743 pKa = 11.84ISSDD747 pKa = 2.9PTFATSTLLYY757 pKa = 10.94DD758 pKa = 4.49GANPNNANSNFFSVSLPNPFKK779 pKa = 10.75LARR782 pKa = 11.84GTTYY786 pKa = 10.06FVRR789 pKa = 11.84VYY791 pKa = 9.86PYY793 pKa = 10.87GGGGTIFDD801 pKa = 3.98SFGFLATCLADD812 pKa = 3.53TDD814 pKa = 3.96GDD816 pKa = 4.37AIANQLDD823 pKa = 4.16LDD825 pKa = 4.32SDD827 pKa = 4.63GEE829 pKa = 4.49GCPDD833 pKa = 3.3AVEE836 pKa = 4.51GGGPFKK842 pKa = 10.74LTDD845 pKa = 3.77LVSSTMAGGNSGTGFNGSSPSPVNQNLGTTVGATGIPTVAGTGQTLGISQTPGLNGCTDD904 pKa = 3.7SDD906 pKa = 3.93ADD908 pKa = 4.16GYY910 pKa = 11.55DD911 pKa = 3.69DD912 pKa = 6.22VVDD915 pKa = 5.79LDD917 pKa = 4.81DD918 pKa = 6.35DD919 pKa = 4.19NDD921 pKa = 5.13GILDD925 pKa = 3.99TAEE928 pKa = 4.26MACQLVNNGDD938 pKa = 3.69FSSISAGWTFTGNVGVFSTDD958 pKa = 2.43SKK960 pKa = 11.55LYY962 pKa = 10.51FNGSNTTPNGVASQVIQTRR981 pKa = 11.84VGQRR985 pKa = 11.84ATLTFEE991 pKa = 4.1MLASGGNSGSVGVQVDD1007 pKa = 3.9VLDD1010 pKa = 3.91VSTGSVLATTSLQRR1024 pKa = 11.84AANTPLVNGALGFVATGTTTRR1045 pKa = 11.84IRR1047 pKa = 11.84FTDD1050 pKa = 3.5ISQATLGIDD1059 pKa = 3.45VQLDD1063 pKa = 3.67NVSILYY1069 pKa = 10.46CDD1071 pKa = 3.8TDD1073 pKa = 3.45NDD1075 pKa = 5.24GIPNSLDD1082 pKa = 3.51LDD1084 pKa = 4.05SDD1086 pKa = 4.12ADD1088 pKa = 3.81GCADD1092 pKa = 3.32AVEE1095 pKa = 4.95GGGSFRR1101 pKa = 11.84VTDD1104 pKa = 4.44LVDD1107 pKa = 3.7SDD1109 pKa = 3.91MDD1111 pKa = 4.03GGSTSVSQNLGDD1123 pKa = 3.87VVGSTTTTMGVPVTAGTGQTVGISQNGALNEE1154 pKa = 4.42CTDD1157 pKa = 3.35SDD1159 pKa = 4.07ADD1161 pKa = 4.02GVVDD1165 pKa = 6.71LSDD1168 pKa = 5.74LDD1170 pKa = 4.57DD1171 pKa = 5.79DD1172 pKa = 5.02NDD1174 pKa = 5.11GILDD1178 pKa = 3.75TAEE1181 pKa = 4.42CPNLGAWNVYY1191 pKa = 10.01AFARR1195 pKa = 11.84TASTFATNVQVIVTGFSSSTVTWNQTIAGTFNNGGFNWTPIASNVMPDD1243 pKa = 3.45ANGKK1247 pKa = 7.7ITATISSVGANNAFLLSDD1265 pKa = 4.14AFLFTNGTSSVVVDD1279 pKa = 3.74NAGAGYY1285 pKa = 10.27SKK1287 pKa = 9.78TGTWSVQTFAGNFGTSEE1304 pKa = 4.34DD1305 pKa = 4.75FIASPFAGQTSTFSLTVAKK1324 pKa = 10.44CDD1326 pKa = 3.47TDD1328 pKa = 3.5GDD1330 pKa = 4.42NIPNHH1335 pKa = 6.92IDD1337 pKa = 3.56LDD1339 pKa = 4.18SDD1341 pKa = 4.05GDD1343 pKa = 3.92GCADD1347 pKa = 4.65AIEE1350 pKa = 4.94GGANFTVANLVSSTMAGGSSNVTSNLGNVVGNTVTTMGVPVTAGTGQTVGTSQNGAVQDD1409 pKa = 4.1AACPQPDD1416 pKa = 3.69YY1417 pKa = 11.61DD1418 pKa = 4.47GDD1420 pKa = 4.2GVLDD1424 pKa = 3.96STDD1427 pKa = 4.79LDD1429 pKa = 4.46DD1430 pKa = 6.93DD1431 pKa = 4.23NDD1433 pKa = 5.09GILDD1437 pKa = 3.92TVEE1440 pKa = 5.63DD1441 pKa = 4.13PACEE1445 pKa = 3.78KK1446 pKa = 10.41VANGSGQNNLAGWTVTGNVVSNTNPQIIFNNNEE1479 pKa = 3.72TPATGVLSQMISTVPNQPLQVKK1501 pKa = 9.88FDD1503 pKa = 3.71MVGFGSNTAGNASLRR1518 pKa = 11.84VDD1520 pKa = 3.73VLTGSTVIATKK1531 pKa = 9.93TVTKK1535 pKa = 10.33TFGNPFTNEE1544 pKa = 3.84TLSFLPKK1551 pKa = 9.99TNQTTIRR1558 pKa = 11.84FTDD1561 pKa = 4.15LSTVTSQFDD1570 pKa = 4.11PIISGISVVGACGLDD1585 pKa = 3.28SDD1587 pKa = 4.74NDD1589 pKa = 4.73GIINSLDD1596 pKa = 3.74LDD1598 pKa = 4.16SDD1600 pKa = 4.21GDD1602 pKa = 4.0GCPDD1606 pKa = 3.5ALEE1609 pKa = 4.69GGAAFTTSNLVSSTMAGGSTNVTKK1633 pKa = 10.75NLPTPVGSGTATMGVPVTAGTGQTVGYY1660 pKa = 8.69SQNSSLNEE1668 pKa = 4.49CIDD1671 pKa = 3.46TDD1673 pKa = 3.99GDD1675 pKa = 4.03GVADD1679 pKa = 5.27INDD1682 pKa = 4.41LDD1684 pKa = 4.88DD1685 pKa = 6.0DD1686 pKa = 4.46NDD1688 pKa = 4.89GILDD1692 pKa = 3.79VAEE1695 pKa = 4.46VSCVEE1700 pKa = 4.73LIQNGVFPTTGGSVDD1715 pKa = 4.13VLAGWTVGGTYY1726 pKa = 10.05SANYY1730 pKa = 7.63PSTYY1734 pKa = 10.16GRR1736 pKa = 11.84VAFVPDD1742 pKa = 3.56GLYY1745 pKa = 10.49FLRR1748 pKa = 11.84DD1749 pKa = 3.37NVTSFISQPISGIYY1763 pKa = 9.58PGGTIMLNGLAWQNTSSVSDD1783 pKa = 3.63PAGQSVLEE1791 pKa = 4.1VKK1793 pKa = 11.0YY1794 pKa = 11.15NGVTYY1799 pKa = 8.91ATVNTGLLNSPTIVGNNGATVNITSLPGVILGAVSAKK1836 pKa = 10.11SNLVITLPTGVPPSGVFQLLFTGGPSPGLADD1867 pKa = 5.72DD1868 pKa = 4.19IAIQSVSSLSCVNADD1883 pKa = 3.89ADD1885 pKa = 3.71NDD1887 pKa = 4.88GIINSLDD1894 pKa = 3.74LDD1896 pKa = 4.16SDD1898 pKa = 4.21GDD1900 pKa = 3.99GCPDD1904 pKa = 4.37AIEE1907 pKa = 4.7GGAAFITSNLVSSTMSGGNSGAGFNGTSPVPVTQNLGNTVGSTTATMGVPTIAGMGQTIGISQNGAVNGCIDD1979 pKa = 3.51TDD1981 pKa = 3.83GDD1983 pKa = 4.23GVLDD1987 pKa = 6.16LDD1989 pKa = 6.16DD1990 pKa = 6.52LDD1992 pKa = 5.79DD1993 pKa = 5.8DD1994 pKa = 4.9NDD1996 pKa = 5.04GILDD2000 pKa = 3.8TVEE2003 pKa = 5.03EE2004 pKa = 4.65GDD2006 pKa = 4.11CEE2008 pKa = 4.63KK2009 pKa = 10.61IINGSGANGFTGWTKK2024 pKa = 10.23TGNVFYY2030 pKa = 11.07NLLPQFSFNYY2040 pKa = 9.66GDD2042 pKa = 3.71SAPTGVLSQTISTIPNQGLQLKK2064 pKa = 9.17FNAVGWGGNTAGNVSVRR2081 pKa = 11.84VDD2083 pKa = 3.32VLAGSTVIATKK2094 pKa = 9.93TVTKK2098 pKa = 10.18TFGIPWTIEE2107 pKa = 3.92TLNFTPTSTSTTIRR2121 pKa = 11.84FTDD2124 pKa = 3.41VSTVATQFDD2133 pKa = 4.81FIINGVSVPGACDD2146 pKa = 3.72LDD2148 pKa = 4.78GDD2150 pKa = 4.84GIVNSLDD2157 pKa = 3.74LDD2159 pKa = 3.95SDD2161 pKa = 4.1GDD2163 pKa = 3.93GCADD2167 pKa = 3.55ALEE2170 pKa = 4.94GGAAFTTSNLVSSTMAGGSTNVTKK2194 pKa = 10.75NLPTPVGSTTATMGVPTIAGTGQTTGTSQNSSAKK2228 pKa = 10.65DD2229 pKa = 3.32PACCLVTGIAPTLSATSKK2247 pKa = 11.26ANACPATTVDD2257 pKa = 4.29LSTITVSNAPASTSLTWHH2275 pKa = 6.6TSATATAANRR2285 pKa = 11.84ITSVSAVAAGTYY2297 pKa = 9.33FAALYY2302 pKa = 9.41DD2303 pKa = 4.1ASTDD2307 pKa = 3.97CYY2309 pKa = 10.53SGSATTPVTAVTAACCSATLAPALSATTKK2338 pKa = 11.03ANTCPATTVDD2348 pKa = 4.53LSTITATNQPASSTLTWHH2366 pKa = 6.47TSSPATASNRR2376 pKa = 11.84ITNITSVTAGTYY2388 pKa = 9.33FAAFFDD2394 pKa = 3.78ATANCFSGTAVTQVTATTTVCCSATLAPALSASSKK2429 pKa = 11.26ANACPATTVDD2439 pKa = 4.52LSTITATNQPASTTLTWHH2457 pKa = 6.32TGSPATASNRR2467 pKa = 11.84ITNISSVTAGTYY2479 pKa = 9.38FAAFFDD2485 pKa = 3.78ATANCFSGTAVTQVTATTTVCCSATLAPALSATTKK2520 pKa = 11.03ANTCPATTVDD2530 pKa = 4.53LSTITATNQPASSTLTWHH2548 pKa = 6.47TGSPATASNRR2558 pKa = 11.84ITNITSVTAGTYY2570 pKa = 9.33FAAFFDD2576 pKa = 3.78ATANCFSGTAVTQVTATTTVCCSATLAPALSATSKK2611 pKa = 11.35ANTCPATTVDD2621 pKa = 4.53LSTITATNQPASTTLTWHH2639 pKa = 6.6TGTPATAANRR2649 pKa = 11.84ITNITSVTAGTYY2661 pKa = 9.33FAAFFDD2667 pKa = 3.78ATANCFSGTAVTQVTATTVACCPNPSVGGSLTLVGTLPLCSVSNQGVLTLSGQTGTVVKK2726 pKa = 9.5WQTSTNGGTSWTDD2739 pKa = 2.87IGNTAGLTQISFSNAQNNQQFRR2761 pKa = 11.84AVVNAGGSCADD2772 pKa = 3.55ANSAPFVTLTSASACSVDD2790 pKa = 4.86CDD2792 pKa = 4.24VKK2794 pKa = 10.99PGTINKK2800 pKa = 9.01NN2801 pKa = 3.11

Molecular weight:
283.39 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A4Q2UM58|A0A4Q2UM58_9BACT Uncharacterized protein OS=Spirosoma sordidisoli OX=2502893 GN=EQG79_00545 PE=4 SV=1
MM1 pKa = 7.48IIKK4 pKa = 10.27VGDD7 pKa = 3.54MVKK10 pKa = 10.51EE11 pKa = 3.93MRR13 pKa = 11.84RR14 pKa = 11.84TVGLHH19 pKa = 3.98QPRR22 pKa = 11.84PFSLVFVTGDD32 pKa = 3.03RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84GRR37 pKa = 11.84GGQIRR42 pKa = 11.84EE43 pKa = 3.86LHH45 pKa = 6.12KK46 pKa = 10.97AVLLTKK52 pKa = 10.3QRR54 pKa = 11.84TEE56 pKa = 3.53NRR58 pKa = 11.84TVNLQPLGTNDD69 pKa = 4.88CICVHH74 pKa = 7.05LDD76 pKa = 3.87LILYY80 pKa = 7.59FNQIPVAA87 pKa = 4.04

Molecular weight:
9.98 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6027

0

6027

2154023

22

4942

357.4

39.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.864 ± 0.034

0.732 ± 0.009

5.473 ± 0.026

4.954 ± 0.034

4.255 ± 0.02

7.365 ± 0.031

1.782 ± 0.017

5.355 ± 0.023

4.248 ± 0.03

10.182 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.046 ± 0.016

4.392 ± 0.033

4.899 ± 0.024

4.625 ± 0.021

6.039 ± 0.026

6.042 ± 0.033

6.629 ± 0.042

7.029 ± 0.023

1.328 ± 0.015

3.76 ± 0.019

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski