Spirosoma sordidisoli
Average proteome isoelectric point is 6.86
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6027 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4Q2UGD5|A0A4Q2UGD5_9BACT AAA family ATPase OS=Spirosoma sordidisoli OX=2502893 GN=EQG79_22305 PE=4 SV=1
MM1 pKa = 8.09 DD2 pKa = 5.87 LFTFKK7 pKa = 10.12 TYY9 pKa = 10.92 SKK11 pKa = 10.31 LQVRR15 pKa = 11.84 TLLRR19 pKa = 11.84 ALCALDD25 pKa = 3.53 WPGLKK30 pKa = 9.86 PAVIRR35 pKa = 11.84 FVVLCAFMLLNMGWPALAEE54 pKa = 3.99 RR55 pKa = 11.84 KK56 pKa = 8.93 PAVLTAVRR64 pKa = 11.84 SYY66 pKa = 10.09 TGTVAPEE73 pKa = 4.06 PVGHH77 pKa = 7.12 KK78 pKa = 10.55 SPATASFMRR87 pKa = 11.84 ARR89 pKa = 11.84 SALALDD95 pKa = 3.77 SDD97 pKa = 4.56 GDD99 pKa = 4.06 NVDD102 pKa = 4.97 DD103 pKa = 6.46 SVDD106 pKa = 4.32 LDD108 pKa = 4.48 DD109 pKa = 6.83 DD110 pKa = 4.16 NDD112 pKa = 5.13 GILDD116 pKa = 4.0 TAEE119 pKa = 4.27 MTCQLVTNGDD129 pKa = 3.85 FASTTGGWSFTGNVGITGGTLFFNGTNTTPDD160 pKa = 4.02 GIATQDD166 pKa = 2.74 IQTRR170 pKa = 11.84 AGKK173 pKa = 10.03 RR174 pKa = 11.84 AMLTFDD180 pKa = 4.29 FVAAGAGTGTIGVTADD196 pKa = 3.46 VLDD199 pKa = 4.04 VSTGTVLGTTSLTSTAGTPSSNISIPFTATGSSIRR234 pKa = 11.84 IRR236 pKa = 11.84 FTDD239 pKa = 3.46 ISQATLSIDD248 pKa = 3.54 VAIDD252 pKa = 3.42 NVSILYY258 pKa = 10.45 CDD260 pKa = 3.8 TDD262 pKa = 3.49 NDD264 pKa = 5.27 GIPDD268 pKa = 4.07 SLDD271 pKa = 3.5 LDD273 pKa = 4.06 SDD275 pKa = 4.14 GDD277 pKa = 3.92 GCADD281 pKa = 4.36 AIEE284 pKa = 4.89 GGGPFRR290 pKa = 11.84 STNLVNSTMAGGNSGVGYY308 pKa = 10.4 NGISTSPVTQNLGISVGATGIPTVAGTGQTVGFSQFRR345 pKa = 11.84 SLNGCIDD352 pKa = 3.9 SEE354 pKa = 4.82 DD355 pKa = 3.6 DD356 pKa = 3.44 TVADD360 pKa = 5.36 VEE362 pKa = 5.91 DD363 pKa = 5.49 LDD365 pKa = 5.6 DD366 pKa = 5.93 DD367 pKa = 4.41 NDD369 pKa = 5.09 GILDD373 pKa = 3.98 TDD375 pKa = 3.89 EE376 pKa = 4.86 MNCQLIEE383 pKa = 4.32 NGDD386 pKa = 3.91 FASTTAGWSFTNNVGITGGFLFFNGSDD413 pKa = 3.47 VTPNGIATQDD423 pKa = 2.91 IQTRR427 pKa = 11.84 AGQRR431 pKa = 11.84 AMLTFDD437 pKa = 4.26 FVAAGAGAGLVGVRR451 pKa = 11.84 IDD453 pKa = 3.7 VLDD456 pKa = 3.75 VSTGTVLATTSLLRR470 pKa = 11.84 TANTPTVTVPLAFTATGTSTRR491 pKa = 11.84 IRR493 pKa = 11.84 FTDD496 pKa = 3.44 ISQATLVIDD505 pKa = 3.78 ARR507 pKa = 11.84 IDD509 pKa = 3.56 NVSILYY515 pKa = 10.45 CDD517 pKa = 3.64 TDD519 pKa = 3.33 NDD521 pKa = 4.92 GIANSLDD528 pKa = 3.76 LDD530 pKa = 4.15 SDD532 pKa = 4.21 GDD534 pKa = 3.98 GCPDD538 pKa = 3.36 AVEE541 pKa = 4.96 GGASFKK547 pKa = 11.11 VADD550 pKa = 4.77 LVNSILPGGNSGTGYY565 pKa = 10.21 IGMSTSPVTQNLGTTVGTNGVPTVAGTGQTVGVSQLAGLNGCTDD609 pKa = 3.56 SDD611 pKa = 4.27 SDD613 pKa = 3.92 TVADD617 pKa = 5.83 IEE619 pKa = 5.93 DD620 pKa = 4.75 LDD622 pKa = 4.96 DD623 pKa = 5.86 DD624 pKa = 4.73 NDD626 pKa = 5.14 GILDD630 pKa = 3.9 TAEE633 pKa = 4.18 NTCMLPLTGTPHH645 pKa = 7.65 LSWDD649 pKa = 3.67 NNGIPLPGTLQINSLSPVVLEE670 pKa = 4.38 TTSITTGPGITLGSSGDD687 pKa = 3.16 SWVISGATATNLAGAVAGNDD707 pKa = 3.3 YY708 pKa = 9.96 MQFTMATSATATVDD722 pKa = 3.39 YY723 pKa = 10.55 TLDD726 pKa = 3.03 QWASFQSTAPFQKK739 pKa = 9.6 IGIRR743 pKa = 11.84 ISSDD747 pKa = 2.9 PTFATSTLLYY757 pKa = 10.94 DD758 pKa = 4.49 GANPNNANSNFFSVSLPNPFKK779 pKa = 10.75 LARR782 pKa = 11.84 GTTYY786 pKa = 10.06 FVRR789 pKa = 11.84 VYY791 pKa = 9.86 PYY793 pKa = 10.87 GGGGTIFDD801 pKa = 3.98 SFGFLATCLADD812 pKa = 3.53 TDD814 pKa = 3.96 GDD816 pKa = 4.37 AIANQLDD823 pKa = 4.16 LDD825 pKa = 4.32 SDD827 pKa = 4.63 GEE829 pKa = 4.49 GCPDD833 pKa = 3.3 AVEE836 pKa = 4.51 GGGPFKK842 pKa = 10.74 LTDD845 pKa = 3.77 LVSSTMAGGNSGTGFNGSSPSPVNQNLGTTVGATGIPTVAGTGQTLGISQTPGLNGCTDD904 pKa = 3.7 SDD906 pKa = 3.93 ADD908 pKa = 4.16 GYY910 pKa = 11.55 DD911 pKa = 3.69 DD912 pKa = 6.22 VVDD915 pKa = 5.79 LDD917 pKa = 4.81 DD918 pKa = 6.35 DD919 pKa = 4.19 NDD921 pKa = 5.13 GILDD925 pKa = 3.99 TAEE928 pKa = 4.26 MACQLVNNGDD938 pKa = 3.69 FSSISAGWTFTGNVGVFSTDD958 pKa = 2.43 SKK960 pKa = 11.55 LYY962 pKa = 10.51 FNGSNTTPNGVASQVIQTRR981 pKa = 11.84 VGQRR985 pKa = 11.84 ATLTFEE991 pKa = 4.1 MLASGGNSGSVGVQVDD1007 pKa = 3.9 VLDD1010 pKa = 3.91 VSTGSVLATTSLQRR1024 pKa = 11.84 AANTPLVNGALGFVATGTTTRR1045 pKa = 11.84 IRR1047 pKa = 11.84 FTDD1050 pKa = 3.5 ISQATLGIDD1059 pKa = 3.45 VQLDD1063 pKa = 3.67 NVSILYY1069 pKa = 10.46 CDD1071 pKa = 3.8 TDD1073 pKa = 3.45 NDD1075 pKa = 5.24 GIPNSLDD1082 pKa = 3.51 LDD1084 pKa = 4.05 SDD1086 pKa = 4.12 ADD1088 pKa = 3.81 GCADD1092 pKa = 3.32 AVEE1095 pKa = 4.95 GGGSFRR1101 pKa = 11.84 VTDD1104 pKa = 4.44 LVDD1107 pKa = 3.7 SDD1109 pKa = 3.91 MDD1111 pKa = 4.03 GGSTSVSQNLGDD1123 pKa = 3.87 VVGSTTTTMGVPVTAGTGQTVGISQNGALNEE1154 pKa = 4.42 CTDD1157 pKa = 3.35 SDD1159 pKa = 4.07 ADD1161 pKa = 4.02 GVVDD1165 pKa = 6.71 LSDD1168 pKa = 5.74 LDD1170 pKa = 4.57 DD1171 pKa = 5.79 DD1172 pKa = 5.02 NDD1174 pKa = 5.11 GILDD1178 pKa = 3.75 TAEE1181 pKa = 4.42 CPNLGAWNVYY1191 pKa = 10.01 AFARR1195 pKa = 11.84 TASTFATNVQVIVTGFSSSTVTWNQTIAGTFNNGGFNWTPIASNVMPDD1243 pKa = 3.45 ANGKK1247 pKa = 7.7 ITATISSVGANNAFLLSDD1265 pKa = 4.14 AFLFTNGTSSVVVDD1279 pKa = 3.74 NAGAGYY1285 pKa = 10.27 SKK1287 pKa = 9.78 TGTWSVQTFAGNFGTSEE1304 pKa = 4.34 DD1305 pKa = 4.75 FIASPFAGQTSTFSLTVAKK1324 pKa = 10.44 CDD1326 pKa = 3.47 TDD1328 pKa = 3.5 GDD1330 pKa = 4.42 NIPNHH1335 pKa = 6.92 IDD1337 pKa = 3.56 LDD1339 pKa = 4.18 SDD1341 pKa = 4.05 GDD1343 pKa = 3.92 GCADD1347 pKa = 4.65 AIEE1350 pKa = 4.94 GGANFTVANLVSSTMAGGSSNVTSNLGNVVGNTVTTMGVPVTAGTGQTVGTSQNGAVQDD1409 pKa = 4.1 AACPQPDD1416 pKa = 3.69 YY1417 pKa = 11.61 DD1418 pKa = 4.47 GDD1420 pKa = 4.2 GVLDD1424 pKa = 3.96 STDD1427 pKa = 4.79 LDD1429 pKa = 4.46 DD1430 pKa = 6.93 DD1431 pKa = 4.23 NDD1433 pKa = 5.09 GILDD1437 pKa = 3.92 TVEE1440 pKa = 5.63 DD1441 pKa = 4.13 PACEE1445 pKa = 3.78 KK1446 pKa = 10.41 VANGSGQNNLAGWTVTGNVVSNTNPQIIFNNNEE1479 pKa = 3.72 TPATGVLSQMISTVPNQPLQVKK1501 pKa = 9.88 FDD1503 pKa = 3.71 MVGFGSNTAGNASLRR1518 pKa = 11.84 VDD1520 pKa = 3.73 VLTGSTVIATKK1531 pKa = 9.93 TVTKK1535 pKa = 10.33 TFGNPFTNEE1544 pKa = 3.84 TLSFLPKK1551 pKa = 9.99 TNQTTIRR1558 pKa = 11.84 FTDD1561 pKa = 4.15 LSTVTSQFDD1570 pKa = 4.11 PIISGISVVGACGLDD1585 pKa = 3.28 SDD1587 pKa = 4.74 NDD1589 pKa = 4.73 GIINSLDD1596 pKa = 3.74 LDD1598 pKa = 4.16 SDD1600 pKa = 4.21 GDD1602 pKa = 4.0 GCPDD1606 pKa = 3.5 ALEE1609 pKa = 4.69 GGAAFTTSNLVSSTMAGGSTNVTKK1633 pKa = 10.75 NLPTPVGSGTATMGVPVTAGTGQTVGYY1660 pKa = 8.69 SQNSSLNEE1668 pKa = 4.49 CIDD1671 pKa = 3.46 TDD1673 pKa = 3.99 GDD1675 pKa = 4.03 GVADD1679 pKa = 5.27 INDD1682 pKa = 4.41 LDD1684 pKa = 4.88 DD1685 pKa = 6.0 DD1686 pKa = 4.46 NDD1688 pKa = 4.89 GILDD1692 pKa = 3.79 VAEE1695 pKa = 4.46 VSCVEE1700 pKa = 4.73 LIQNGVFPTTGGSVDD1715 pKa = 4.13 VLAGWTVGGTYY1726 pKa = 10.05 SANYY1730 pKa = 7.63 PSTYY1734 pKa = 10.16 GRR1736 pKa = 11.84 VAFVPDD1742 pKa = 3.56 GLYY1745 pKa = 10.49 FLRR1748 pKa = 11.84 DD1749 pKa = 3.37 NVTSFISQPISGIYY1763 pKa = 9.58 PGGTIMLNGLAWQNTSSVSDD1783 pKa = 3.63 PAGQSVLEE1791 pKa = 4.1 VKK1793 pKa = 11.0 YY1794 pKa = 11.15 NGVTYY1799 pKa = 8.91 ATVNTGLLNSPTIVGNNGATVNITSLPGVILGAVSAKK1836 pKa = 10.11 SNLVITLPTGVPPSGVFQLLFTGGPSPGLADD1867 pKa = 5.72 DD1868 pKa = 4.19 IAIQSVSSLSCVNADD1883 pKa = 3.89 ADD1885 pKa = 3.71 NDD1887 pKa = 4.88 GIINSLDD1894 pKa = 3.74 LDD1896 pKa = 4.16 SDD1898 pKa = 4.21 GDD1900 pKa = 3.99 GCPDD1904 pKa = 4.37 AIEE1907 pKa = 4.7 GGAAFITSNLVSSTMSGGNSGAGFNGTSPVPVTQNLGNTVGSTTATMGVPTIAGMGQTIGISQNGAVNGCIDD1979 pKa = 3.51 TDD1981 pKa = 3.83 GDD1983 pKa = 4.23 GVLDD1987 pKa = 6.16 LDD1989 pKa = 6.16 DD1990 pKa = 6.52 LDD1992 pKa = 5.79 DD1993 pKa = 5.8 DD1994 pKa = 4.9 NDD1996 pKa = 5.04 GILDD2000 pKa = 3.8 TVEE2003 pKa = 5.03 EE2004 pKa = 4.65 GDD2006 pKa = 4.11 CEE2008 pKa = 4.63 KK2009 pKa = 10.61 IINGSGANGFTGWTKK2024 pKa = 10.23 TGNVFYY2030 pKa = 11.07 NLLPQFSFNYY2040 pKa = 9.66 GDD2042 pKa = 3.71 SAPTGVLSQTISTIPNQGLQLKK2064 pKa = 9.17 FNAVGWGGNTAGNVSVRR2081 pKa = 11.84 VDD2083 pKa = 3.32 VLAGSTVIATKK2094 pKa = 9.93 TVTKK2098 pKa = 10.18 TFGIPWTIEE2107 pKa = 3.92 TLNFTPTSTSTTIRR2121 pKa = 11.84 FTDD2124 pKa = 3.41 VSTVATQFDD2133 pKa = 4.81 FIINGVSVPGACDD2146 pKa = 3.72 LDD2148 pKa = 4.78 GDD2150 pKa = 4.84 GIVNSLDD2157 pKa = 3.74 LDD2159 pKa = 3.95 SDD2161 pKa = 4.1 GDD2163 pKa = 3.93 GCADD2167 pKa = 3.55 ALEE2170 pKa = 4.94 GGAAFTTSNLVSSTMAGGSTNVTKK2194 pKa = 10.75 NLPTPVGSTTATMGVPTIAGTGQTTGTSQNSSAKK2228 pKa = 10.65 DD2229 pKa = 3.32 PACCLVTGIAPTLSATSKK2247 pKa = 11.26 ANACPATTVDD2257 pKa = 4.29 LSTITVSNAPASTSLTWHH2275 pKa = 6.6 TSATATAANRR2285 pKa = 11.84 ITSVSAVAAGTYY2297 pKa = 9.33 FAALYY2302 pKa = 9.41 DD2303 pKa = 4.1 ASTDD2307 pKa = 3.97 CYY2309 pKa = 10.53 SGSATTPVTAVTAACCSATLAPALSATTKK2338 pKa = 11.03 ANTCPATTVDD2348 pKa = 4.53 LSTITATNQPASSTLTWHH2366 pKa = 6.47 TSSPATASNRR2376 pKa = 11.84 ITNITSVTAGTYY2388 pKa = 9.33 FAAFFDD2394 pKa = 3.78 ATANCFSGTAVTQVTATTTVCCSATLAPALSASSKK2429 pKa = 11.26 ANACPATTVDD2439 pKa = 4.52 LSTITATNQPASTTLTWHH2457 pKa = 6.32 TGSPATASNRR2467 pKa = 11.84 ITNISSVTAGTYY2479 pKa = 9.38 FAAFFDD2485 pKa = 3.78 ATANCFSGTAVTQVTATTTVCCSATLAPALSATTKK2520 pKa = 11.03 ANTCPATTVDD2530 pKa = 4.53 LSTITATNQPASSTLTWHH2548 pKa = 6.47 TGSPATASNRR2558 pKa = 11.84 ITNITSVTAGTYY2570 pKa = 9.33 FAAFFDD2576 pKa = 3.78 ATANCFSGTAVTQVTATTTVCCSATLAPALSATSKK2611 pKa = 11.35 ANTCPATTVDD2621 pKa = 4.53 LSTITATNQPASTTLTWHH2639 pKa = 6.6 TGTPATAANRR2649 pKa = 11.84 ITNITSVTAGTYY2661 pKa = 9.33 FAAFFDD2667 pKa = 3.78 ATANCFSGTAVTQVTATTVACCPNPSVGGSLTLVGTLPLCSVSNQGVLTLSGQTGTVVKK2726 pKa = 9.5 WQTSTNGGTSWTDD2739 pKa = 2.87 IGNTAGLTQISFSNAQNNQQFRR2761 pKa = 11.84 AVVNAGGSCADD2772 pKa = 3.55 ANSAPFVTLTSASACSVDD2790 pKa = 4.86 CDD2792 pKa = 4.24 VKK2794 pKa = 10.99 PGTINKK2800 pKa = 9.01 NN2801 pKa = 3.11
Molecular weight: 283.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.71
IPC2_protein 3.541
IPC_protein 3.63
Toseland 3.363
ProMoST 3.808
Dawson 3.643
Bjellqvist 3.795
Wikipedia 3.617
Rodwell 3.439
Grimsley 3.261
Solomon 3.643
Lehninger 3.605
Nozaki 3.745
DTASelect 4.101
Thurlkill 3.427
EMBOSS 3.617
Sillero 3.745
Patrickios 0.795
IPC_peptide 3.63
IPC2_peptide 3.719
IPC2.peptide.svr19 3.705
Protein with the highest isoelectric point:
>tr|A0A4Q2UM58|A0A4Q2UM58_9BACT Uncharacterized protein OS=Spirosoma sordidisoli OX=2502893 GN=EQG79_00545 PE=4 SV=1
MM1 pKa = 7.48 IIKK4 pKa = 10.27 VGDD7 pKa = 3.54 MVKK10 pKa = 10.51 EE11 pKa = 3.93 MRR13 pKa = 11.84 RR14 pKa = 11.84 TVGLHH19 pKa = 3.98 QPRR22 pKa = 11.84 PFSLVFVTGDD32 pKa = 3.03 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 GRR37 pKa = 11.84 GGQIRR42 pKa = 11.84 EE43 pKa = 3.86 LHH45 pKa = 6.12 KK46 pKa = 10.97 AVLLTKK52 pKa = 10.3 QRR54 pKa = 11.84 TEE56 pKa = 3.53 NRR58 pKa = 11.84 TVNLQPLGTNDD69 pKa = 4.88 CICVHH74 pKa = 7.05 LDD76 pKa = 3.87 LILYY80 pKa = 7.59 FNQIPVAA87 pKa = 4.04
Molecular weight: 9.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.344
IPC2_protein 9.472
IPC_protein 10.16
Toseland 10.716
ProMoST 10.379
Dawson 10.774
Bjellqvist 10.482
Wikipedia 10.979
Rodwell 10.965
Grimsley 10.804
Solomon 10.935
Lehninger 10.906
Nozaki 10.716
DTASelect 10.467
Thurlkill 10.701
EMBOSS 11.111
Sillero 10.716
Patrickios 10.76
IPC_peptide 10.935
IPC2_peptide 9.663
IPC2.peptide.svr19 8.637
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6027
0
6027
2154023
22
4942
357.4
39.7
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.864 ± 0.034
0.732 ± 0.009
5.473 ± 0.026
4.954 ± 0.034
4.255 ± 0.02
7.365 ± 0.031
1.782 ± 0.017
5.355 ± 0.023
4.248 ± 0.03
10.182 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.046 ± 0.016
4.392 ± 0.033
4.899 ± 0.024
4.625 ± 0.021
6.039 ± 0.026
6.042 ± 0.033
6.629 ± 0.042
7.029 ± 0.023
1.328 ± 0.015
3.76 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here