Methanoregula boonei (strain DSM 21154 / JCM 14090 / 6A8)

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia; Methanomicrobiales; Methanoregulaceae; Methanoregula; Methanoregula boonei

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2450 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A7I7N4|A7I7N4_METB6 Cobalamin (Vitamin B12) biosynthesis CbiG protein OS=Methanoregula boonei (strain DSM 21154 / JCM 14090 / 6A8) OX=456442 GN=Mboo_1227 PE=4 SV=1
MM1 pKa = 8.14DD2 pKa = 6.4DD3 pKa = 4.32DD4 pKa = 4.66EE5 pKa = 4.42EE6 pKa = 4.54TAFEE10 pKa = 4.14EE11 pKa = 4.99LEE13 pKa = 4.27EE14 pKa = 4.45EE15 pKa = 4.54PVILPPAPMGPWFWAVMAMIGILLLMILYY44 pKa = 9.27GQVQGITHH52 pKa = 7.55PIEE55 pKa = 4.47VNLTDD60 pKa = 4.0TQWTLSSYY68 pKa = 11.71ADD70 pKa = 3.48AQGTMVPVKK79 pKa = 10.53DD80 pKa = 3.79GAGSNISFGLPGDD93 pKa = 3.55GTLRR97 pKa = 11.84GMSGCTAYY105 pKa = 10.51SYY107 pKa = 11.12QYY109 pKa = 10.56VRR111 pKa = 11.84NATSIHH117 pKa = 6.4ISDD120 pKa = 4.16GSIISGSCDD129 pKa = 3.76DD130 pKa = 5.16PGTGNATSLYY140 pKa = 10.41FSDD143 pKa = 3.97LTRR146 pKa = 11.84ASGLRR151 pKa = 11.84FRR153 pKa = 11.84SGQIFVSDD161 pKa = 3.31AAGRR165 pKa = 11.84PLLVFDD171 pKa = 5.6LAGTT175 pKa = 3.94

Molecular weight: 18.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A7I4C7|A7I4C7_METB6 ATP-NAD/AcoX kinase OS=Methanoregula boonei (strain DSM 21154 / JCM 14090 / 6A8) OX=456442 GN=Mboo_0064 PE=4 SV=1
MM1 pKa = 7.48ARR3 pKa = 11.84HH4 pKa = 6.09PNVVFRR10 pKa = 11.84DD11 pKa = 3.32IFRR14 pKa = 11.84QLEE17 pKa = 4.34KK18 pKa = 10.62ISLFFSNFPAILYY31 pKa = 10.3SPVRR35 pKa = 11.84CSLAIVQVIEE45 pKa = 4.08MEE47 pKa = 3.88IHH49 pKa = 6.08HH50 pKa = 6.87EE51 pKa = 3.73ISRR54 pKa = 11.84GIVIVTFSGWLDD66 pKa = 3.35TTSAGKK72 pKa = 9.88FLEE75 pKa = 4.37YY76 pKa = 10.43CVLLPPATPIIFDD89 pKa = 3.94LSRR92 pKa = 11.84LSFLSRR98 pKa = 11.84TGLRR102 pKa = 11.84ALLRR106 pKa = 11.84FRR108 pKa = 11.84NMRR111 pKa = 11.84ASTGSVVVIAGSRR124 pKa = 11.84GFVDD128 pKa = 3.48TALKK132 pKa = 9.77MNRR135 pKa = 11.84FNRR138 pKa = 11.84LFPMYY143 pKa = 10.28LSVADD148 pKa = 5.73AIPAIATAAAPIPRR162 pKa = 11.84VWGGAA167 pKa = 3.29

Molecular weight: 18.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2450

0

2450

727371

29

4079

296.9

32.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.464 ± 0.076

1.361 ± 0.023

5.284 ± 0.043

6.061 ± 0.067

3.917 ± 0.036

7.979 ± 0.057

1.997 ± 0.025

7.19 ± 0.045

4.743 ± 0.048

9.193 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.449 ± 0.025

3.334 ± 0.044

4.942 ± 0.039

2.93 ± 0.033

5.541 ± 0.064

5.957 ± 0.05

6.172 ± 0.073

7.502 ± 0.053

0.979 ± 0.02

3.006 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski