Amino acid dipepetide frequency for Methanoregula boonei (strain DSM 21154 / JCM 14090 / 6A8)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	10.664AlaAla: 10.664 ± 0.178	1.324AlaCys: 1.324 ± 0.046	5.007AlaAsp: 5.007 ± 0.088	5.716AlaGlu: 5.716 ± 0.102	3.601AlaPhe: 3.601 ± 0.108	9.119AlaGly: 9.119 ± 0.133	1.628AlaHis: 1.628 ± 0.043	6.749AlaIle: 6.749 ± 0.119	4.082AlaLys: 4.082 ± 0.093	9.636AlaLeu: 9.636 ± 0.236	2.438AlaMet: 2.438 ± 0.058	2.73AlaAsn: 2.73 ± 0.07	3.66AlaPro: 3.66 ± 0.083	2.724AlaGln: 2.724 ± 0.074	5.014AlaArg: 5.014 ± 0.099	4.674AlaSer: 4.674 ± 0.082	5.202AlaThr: 5.202 ± 0.106	7.332AlaVal: 7.332 ± 0.115	0.917AlaTrp: 0.917 ± 0.045	2.42AlaTyr: 2.42 ± 0.07	0.0AlaXaa: 0.0 ± 0.0
Cys	1.159CysAla: 1.159 ± 0.039	0.258CysCys: 0.258 ± 0.022	0.664CysAsp: 0.664 ± 0.033	0.668CysGlu: 0.668 ± 0.032	0.43CysPhe: 0.43 ± 0.027	1.593CysGly: 1.593 ± 0.057	0.32CysHis: 0.32 ± 0.02	0.927CysIle: 0.927 ± 0.041	0.379CysLys: 0.379 ± 0.025	0.98CysLeu: 0.98 ± 0.047	0.356CysMet: 0.356 ± 0.023	0.4CysAsn: 0.4 ± 0.025	0.913CysPro: 0.913 ± 0.048	0.329CysGln: 0.329 ± 0.021	0.973CysArg: 0.973 ± 0.041	0.85CysSer: 0.85 ± 0.041	1.081CysThr: 1.081 ± 0.044	0.852CysVal: 0.852 ± 0.038	0.129CysTrp: 0.129 ± 0.013	0.342CysTyr: 0.342 ± 0.022	0.0CysXaa: 0.0 ± 0.0
Asp	5.154AspAla: 5.154 ± 0.114	0.646AspCys: 0.646 ± 0.031	2.303AspAsp: 2.303 ± 0.062	3.524AspGlu: 3.524 ± 0.082	1.965AspPhe: 1.965 ± 0.062	3.691AspGly: 3.691 ± 0.079	1.2AspHis: 1.2 ± 0.045	3.987AspIle: 3.987 ± 0.086	2.013AspLys: 2.013 ± 0.064	5.637AspLeu: 5.637 ± 0.095	1.25AspMet: 1.25 ± 0.047	1.683AspAsn: 1.683 ± 0.056	3.458AspPro: 3.458 ± 0.078	1.45AspGln: 1.45 ± 0.044	3.445AspArg: 3.445 ± 0.075	2.45AspSer: 2.45 ± 0.059	3.177AspThr: 3.177 ± 0.067	3.653AspVal: 3.653 ± 0.086	0.547AspTrp: 0.547 ± 0.025	1.603AspTyr: 1.603 ± 0.044	0.0AspXaa: 0.0 ± 0.0
Glu	4.916GluAla: 4.916 ± 0.141	0.767GluCys: 0.767 ± 0.032	2.684GluAsp: 2.684 ± 0.076	4.398GluGlu: 4.398 ± 0.098	2.101GluPhe: 2.101 ± 0.057	3.962GluGly: 3.962 ± 0.079	1.316GluHis: 1.316 ± 0.047	5.38GluIle: 5.38 ± 0.107	4.479GluLys: 4.479 ± 0.09	5.085GluLeu: 5.085 ± 0.096	1.666GluMet: 1.666 ± 0.051	2.505GluAsn: 2.505 ± 0.059	2.374GluPro: 2.374 ± 0.063	2.215GluGln: 2.215 ± 0.055	3.865GluArg: 3.865 ± 0.081	3.165GluSer: 3.165 ± 0.072	3.36GluThr: 3.36 ± 0.075	3.935GluVal: 3.935 ± 0.085	0.506GluTrp: 0.506 ± 0.024	1.93GluTyr: 1.93 ± 0.05	0.0GluXaa: 0.0 ± 0.0
Phe	3.672PheAla: 3.672 ± 0.079	0.668PheCys: 0.668 ± 0.029	2.278PheAsp: 2.278 ± 0.072	2.18PheGlu: 2.18 ± 0.066	1.963PhePhe: 1.963 ± 0.07	3.235PheGly: 3.235 ± 0.075	0.704PheHis: 0.704 ± 0.03	2.355PheIle: 2.355 ± 0.06	1.133PheLys: 1.133 ± 0.045	3.826PheLeu: 3.826 ± 0.091	0.782PheMet: 0.782 ± 0.034	1.226PheAsn: 1.226 ± 0.061	1.758PhePro: 1.758 ± 0.046	0.819PheGln: 0.819 ± 0.034	1.967PheArg: 1.967 ± 0.058	3.209PheSer: 3.209 ± 0.075	2.56PheThr: 2.56 ± 0.067	3.238PheVal: 3.238 ± 0.069	0.47PheTrp: 0.47 ± 0.029	1.12PheTyr: 1.12 ± 0.036	0.0PheXaa: 0.0 ± 0.0
Gly	6.617GlyAla: 6.617 ± 0.121	1.252GlyCys: 1.252 ± 0.048	3.958GlyAsp: 3.958 ± 0.084	4.511GlyGlu: 4.511 ± 0.088	3.158GlyPhe: 3.158 ± 0.066	5.828GlyGly: 5.828 ± 0.119	1.483GlyHis: 1.483 ± 0.049	7.215GlyIle: 7.215 ± 0.12	4.777GlyLys: 4.777 ± 0.104	6.442GlyLeu: 6.442 ± 0.105	2.355GlyMet: 2.355 ± 0.062	2.912GlyAsn: 2.912 ± 0.093	2.64GlyPro: 2.64 ± 0.066	2.134GlyGln: 2.134 ± 0.057	4.141GlyArg: 4.141 ± 0.091	5.228GlySer: 5.228 ± 0.103	5.817GlyThr: 5.817 ± 0.098	5.488GlyVal: 5.488 ± 0.102	0.947GlyTrp: 0.947 ± 0.034	2.887GlyTyr: 2.887 ± 0.066	0.0GlyXaa: 0.0 ± 0.0
His	1.921HisAla: 1.921 ± 0.049	0.329HisCys: 0.329 ± 0.024	1.136HisAsp: 1.136 ± 0.034	1.197HisGlu: 1.197 ± 0.038	0.756HisPhe: 0.756 ± 0.036	1.548HisGly: 1.548 ± 0.054	0.579HisHis: 0.579 ± 0.03	1.351HisIle: 1.351 ± 0.051	0.751HisLys: 0.751 ± 0.032	1.856HisLeu: 1.856 ± 0.054	0.437HisMet: 0.437 ± 0.024	0.628HisAsn: 0.628 ± 0.028	1.368HisPro: 1.368 ± 0.052	0.565HisGln: 0.565 ± 0.027	1.228HisArg: 1.228 ± 0.044	1.041HisSer: 1.041 ± 0.037	1.145HisThr: 1.145 ± 0.038	1.354HisVal: 1.354 ± 0.048	0.198HisTrp: 0.198 ± 0.017	0.58HisTyr: 0.58 ± 0.03	0.0HisXaa: 0.0 ± 0.0
Ile	7.463IleAla: 7.463 ± 0.107	0.942IleCys: 0.942 ± 0.035	3.951IleAsp: 3.951 ± 0.075	4.069IleGlu: 4.069 ± 0.097	2.84IlePhe: 2.84 ± 0.071	5.556IleGly: 5.556 ± 0.104	1.485IleHis: 1.485 ± 0.043	4.694IleIle: 4.694 ± 0.1	2.629IleLys: 2.629 ± 0.062	6.907IleLeu: 6.907 ± 0.129	1.362IleMet: 1.362 ± 0.044	2.099IleAsn: 2.099 ± 0.06	4.041IlePro: 4.041 ± 0.082	1.866IleGln: 1.866 ± 0.056	4.672IleArg: 4.672 ± 0.104	4.696IleSer: 4.696 ± 0.09	4.768IleThr: 4.768 ± 0.103	5.53IleVal: 5.53 ± 0.102	0.616IleTrp: 0.616 ± 0.03	1.717IleTyr: 1.717 ± 0.051	0.0IleXaa: 0.0 ± 0.0
Lys	3.772LysAla: 3.772 ± 0.087	0.536LysCys: 0.536 ± 0.026	2.571LysAsp: 2.571 ± 0.068	3.79LysGlu: 3.79 ± 0.084	1.401LysPhe: 1.401 ± 0.045	3.498LysGly: 3.498 ± 0.093	0.741LysHis: 0.741 ± 0.031	3.61LysIle: 3.61 ± 0.082	4.519LysLys: 4.519 ± 0.102	3.078LysLeu: 3.078 ± 0.072	1.313LysMet: 1.313 ± 0.044	2.447LysAsn: 2.447 ± 0.067	1.967LysPro: 1.967 ± 0.054	1.309LysGln: 1.309 ± 0.046	2.457LysArg: 2.457 ± 0.072	2.564LysSer: 2.564 ± 0.062	2.964LysThr: 2.964 ± 0.074	3.029LysVal: 3.029 ± 0.075	0.378LysTrp: 0.378 ± 0.021	1.483LysTyr: 1.483 ± 0.049	0.0LysXaa: 0.0 ± 0.0
Leu	9.797LeuAla: 9.797 ± 0.166	1.257LeuCys: 1.257 ± 0.045	4.984LeuAsp: 4.984 ± 0.094	5.11LeuGlu: 5.11 ± 0.103	4.111LeuPhe: 4.111 ± 0.1	6.698LeuGly: 6.698 ± 0.107	1.769LeuHis: 1.769 ± 0.045	6.22LeuIle: 6.22 ± 0.127	4.135LeuLys: 4.135 ± 0.089	8.971LeuLeu: 8.971 ± 0.146	2.123LeuMet: 2.123 ± 0.053	2.899LeuAsn: 2.899 ± 0.067	4.636LeuPro: 4.636 ± 0.092	2.33LeuGln: 2.33 ± 0.061	4.699LeuArg: 4.699 ± 0.103	6.431LeuSer: 6.431 ± 0.106	5.154LeuThr: 5.154 ± 0.097	7.287LeuVal: 7.287 ± 0.121	0.83LeuTrp: 0.83 ± 0.038	2.49LeuTyr: 2.49 ± 0.068	0.0LeuXaa: 0.0 ± 0.0
Met	2.341MetAla: 2.341 ± 0.056	0.253MetCys: 0.253 ± 0.019	1.404MetAsp: 1.404 ± 0.047	1.441MetGlu: 1.441 ± 0.046	0.734MetPhe: 0.734 ± 0.034	1.915MetGly: 1.915 ± 0.052	0.511MetHis: 0.511 ± 0.028	1.749MetIle: 1.749 ± 0.051	1.475MetLys: 1.475 ± 0.051	2.091MetLeu: 2.091 ± 0.059	0.683MetMet: 0.683 ± 0.032	0.898MetAsn: 0.898 ± 0.038	1.32MetPro: 1.32 ± 0.04	0.894MetGln: 0.894 ± 0.033	1.217MetArg: 1.217 ± 0.042	1.328MetSer: 1.328 ± 0.043	1.692MetThr: 1.692 ± 0.058	1.763MetVal: 1.763 ± 0.047	0.139MetTrp: 0.139 ± 0.014	0.642MetTyr: 0.642 ± 0.033	0.0MetXaa: 0.0 ± 0.0
Asn	3.133AsnAla: 3.133 ± 0.077	0.407AsnCys: 0.407 ± 0.026	1.637AsnAsp: 1.637 ± 0.05	1.819AsnGlu: 1.819 ± 0.052	1.211AsnPhe: 1.211 ± 0.041	2.836AsnGly: 2.836 ± 0.087	0.588AsnHis: 0.588 ± 0.029	2.295AsnIle: 2.295 ± 0.068	1.175AsnLys: 1.175 ± 0.045	3.15AsnLeu: 3.15 ± 0.074	0.745AsnMet: 0.745 ± 0.032	1.195AsnAsn: 1.195 ± 0.05	2.391AsnPro: 2.391 ± 0.065	1.057AsnGln: 1.057 ± 0.045	1.988AsnArg: 1.988 ± 0.054	1.752AsnSer: 1.752 ± 0.073	2.189AsnThr: 2.189 ± 0.075	2.443AsnVal: 2.443 ± 0.071	0.334AsnTrp: 0.334 ± 0.03	0.997AsnTyr: 0.997 ± 0.046	0.0AsnXaa: 0.0 ± 0.0
Pro	5.514ProAla: 5.514 ± 0.095	0.558ProCys: 0.558 ± 0.035	3.399ProAsp: 3.399 ± 0.074	3.68ProGlu: 3.68 ± 0.083	1.984ProPhe: 1.984 ± 0.056	4.716ProGly: 4.716 ± 0.101	0.984ProHis: 0.984 ± 0.036	2.257ProIle: 2.257 ± 0.063	1.811ProLys: 1.811 ± 0.049	4.267ProLeu: 4.267 ± 0.089	1.012ProMet: 1.012 ± 0.039	1.133ProAsn: 1.133 ± 0.039	2.242ProPro: 2.242 ± 0.063	1.493ProGln: 1.493 ± 0.052	2.09ProArg: 2.09 ± 0.056	2.67ProSer: 2.67 ± 0.07	2.582ProThr: 2.582 ± 0.097	5.242ProVal: 5.242 ± 0.095	0.448ProTrp: 0.448 ± 0.025	1.342ProTyr: 1.342 ± 0.044	0.0ProXaa: 0.0 ± 0.0
Gln	2.587GlnAla: 2.587 ± 0.063	0.323GlnCys: 0.323 ± 0.022	1.545GlnAsp: 1.545 ± 0.044	1.932GlnGlu: 1.932 ± 0.047	1.152GlnPhe: 1.152 ± 0.039	1.905GlnGly: 1.905 ± 0.055	0.594GlnHis: 0.594 ± 0.032	2.235GlnIle: 2.235 ± 0.061	1.87GlnLys: 1.87 ± 0.056	2.26GlnLeu: 2.26 ± 0.056	0.938GlnMet: 0.938 ± 0.038	1.169GlnAsn: 1.169 ± 0.051	1.229GlnPro: 1.229 ± 0.046	1.18GlnGln: 1.18 ± 0.042	1.482GlnArg: 1.482 ± 0.052	1.58GlnSer: 1.58 ± 0.054	1.629GlnThr: 1.629 ± 0.055	2.255GlnVal: 2.255 ± 0.054	0.296GlnTrp: 0.296 ± 0.025	1.136GlnTyr: 1.136 ± 0.043	0.0GlnXaa: 0.0 ± 0.0
Arg	4.504ArgAla: 4.504 ± 0.103	0.803ArgCys: 0.803 ± 0.033	3.374ArgAsp: 3.374 ± 0.079	4.545ArgGlu: 4.545 ± 0.094	2.33ArgPhe: 2.33 ± 0.056	3.455ArgGly: 3.455 ± 0.071	1.328ArgHis: 1.328 ± 0.04	4.362ArgIle: 4.362 ± 0.088	3.011ArgLys: 3.011 ± 0.074	4.739ArgLeu: 4.739 ± 0.098	1.593ArgMet: 1.593 ± 0.047	1.782ArgAsn: 1.782 ± 0.046	2.3ArgPro: 2.3 ± 0.067	1.963ArgGln: 1.963 ± 0.053	3.034ArgArg: 3.034 ± 0.071	3.008ArgSer: 3.008 ± 0.065	2.869ArgThr: 2.869 ± 0.066	3.752ArgVal: 3.752 ± 0.076	0.575ArgTrp: 0.575 ± 0.03	2.081ArgTyr: 2.081 ± 0.064	0.0ArgXaa: 0.0 ± 0.0
Ser	5.457SerAla: 5.457 ± 0.114	0.806SerCys: 0.806 ± 0.034	3.099SerAsp: 3.099 ± 0.06	2.979SerGlu: 2.979 ± 0.069	2.407SerPhe: 2.407 ± 0.064	6.126SerGly: 6.126 ± 0.121	1.259SerHis: 1.259 ± 0.045	3.518SerIle: 3.518 ± 0.081	1.934SerLys: 1.934 ± 0.06	5.738SerLeu: 5.738 ± 0.101	1.442SerMet: 1.442 ± 0.051	1.633SerAsn: 1.633 ± 0.06	3.463SerPro: 3.463 ± 0.088	1.665SerGln: 1.665 ± 0.059	3.484SerArg: 3.484 ± 0.087	4.043SerSer: 4.043 ± 0.108	3.601SerThr: 3.601 ± 0.089	4.487SerVal: 4.487 ± 0.091	0.72SerTrp: 0.72 ± 0.036	1.706SerTyr: 1.706 ± 0.051	0.0SerXaa: 0.0 ± 0.0
Thr	5.704ThrAla: 5.704 ± 0.13	0.813ThrCys: 0.813 ± 0.039	3.157ThrAsp: 3.157 ± 0.075	3.063ThrGlu: 3.063 ± 0.075	2.293ThrPhe: 2.293 ± 0.058	6.452ThrGly: 6.452 ± 0.12	1.118ThrHis: 1.118 ± 0.045	4.318ThrIle: 4.318 ± 0.08	2.37ThrLys: 2.37 ± 0.068	6.049ThrLeu: 6.049 ± 0.115	1.221ThrMet: 1.221 ± 0.046	1.841ThrAsn: 1.841 ± 0.07	3.653ThrPro: 3.653 ± 0.092	1.47ThrGln: 1.47 ± 0.05	3.033ThrArg: 3.033 ± 0.064	3.665ThrSer: 3.665 ± 0.107	4.087ThrThr: 4.087 ± 0.136	4.905ThrVal: 4.905 ± 0.102	0.583ThrTrp: 0.583 ± 0.031	1.927ThrTyr: 1.927 ± 0.064	0.0ThrXaa: 0.0 ± 0.0
Val	6.772ValAla: 6.772 ± 0.116	1.039ValCys: 1.039 ± 0.043	3.539ValAsp: 3.539 ± 0.078	3.833ValGlu: 3.833 ± 0.082	3.115ValPhe: 3.115 ± 0.062	4.588ValGly: 4.588 ± 0.099	1.504ValHis: 1.504 ± 0.047	5.986ValIle: 5.986 ± 0.095	3.374ValLys: 3.374 ± 0.073	7.282ValLeu: 7.282 ± 0.119	1.923ValMet: 1.923 ± 0.055	2.625ValAsn: 2.625 ± 0.075	4.251ValPro: 4.251 ± 0.094	2.352ValGln: 2.352 ± 0.059	4.079ValArg: 4.079 ± 0.084	4.799ValSer: 4.799 ± 0.08	5.341ValThr: 5.341 ± 0.108	5.814ValVal: 5.814 ± 0.099	0.683ValTrp: 0.683 ± 0.033	2.12ValTyr: 2.12 ± 0.066	0.0ValXaa: 0.0 ± 0.0
Trp	0.697TrpAla: 0.697 ± 0.035	0.154TrpCys: 0.154 ± 0.014	0.549TrpAsp: 0.549 ± 0.03	0.549TrpGlu: 0.549 ± 0.025	0.415TrpPhe: 0.415 ± 0.025	0.641TrpGly: 0.641 ± 0.029	0.184TrpHis: 0.184 ± 0.015	0.781TrpIle: 0.781 ± 0.03	0.542TrpLys: 0.542 ± 0.03	0.906TrpLeu: 0.906 ± 0.036	0.287TrpMet: 0.287 ± 0.019	0.522TrpAsn: 0.522 ± 0.034	0.242TrpPro: 0.242 ± 0.018	0.441TrpGln: 0.441 ± 0.025	0.502TrpArg: 0.502 ± 0.027	0.593TrpSer: 0.593 ± 0.036	0.514TrpThr: 0.514 ± 0.025	0.687TrpVal: 0.687 ± 0.033	0.151TrpTrp: 0.151 ± 0.015	0.437TrpTyr: 0.437 ± 0.03	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.791TyrAla: 2.791 ± 0.068	0.47TyrCys: 0.47 ± 0.027	1.599TyrAsp: 1.599 ± 0.057	1.603TyrGlu: 1.603 ± 0.051	1.197TyrPhe: 1.197 ± 0.044	2.429TyrGly: 2.429 ± 0.062	0.671TyrHis: 0.671 ± 0.031	1.914TyrIle: 1.914 ± 0.056	0.969TyrLys: 0.969 ± 0.034	3.029TyrLeu: 3.029 ± 0.068	0.561TyrMet: 0.561 ± 0.026	1.015TyrAsn: 1.015 ± 0.046	1.519TyrPro: 1.519 ± 0.047	1.041TyrGln: 1.041 ± 0.04	2.04TyrArg: 2.04 ± 0.06	1.823TyrSer: 1.823 ± 0.057	1.989TyrThr: 1.989 ± 0.061	1.976TyrVal: 1.976 ± 0.059	0.326TyrTrp: 0.326 ± 0.024	1.097TyrTyr: 1.097 ± 0.045	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2450 proteins (727371 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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