gamma proteobacterium HIMB55

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Cellvibrionales; Halieaceae; unclassified Halieaceae

Average proteome isoelectric point is 5.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2470 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H3NY45|H3NY45_9GAMM Amino acid carrier protein OS=gamma proteobacterium HIMB55 OX=745014 GN=OMB55_00004860 PE=3 SV=1
MM1 pKa = 7.04NRR3 pKa = 11.84APVFDD8 pKa = 3.74QASYY12 pKa = 11.1ALSVQEE18 pKa = 4.13NTGTTISGISFSDD31 pKa = 3.67PDD33 pKa = 4.34ADD35 pKa = 5.47PITLSISGTDD45 pKa = 3.33SEE47 pKa = 4.86AFSVTSIGSLAFITPPDD64 pKa = 3.91FEE66 pKa = 4.83TPADD70 pKa = 3.49SDD72 pKa = 3.8QNNEE76 pKa = 3.71YY77 pKa = 10.66SITLSAYY84 pKa = 9.83DD85 pKa = 3.74GKK87 pKa = 9.77LTTSVSVVITVTNDD101 pKa = 2.83VSDD104 pKa = 4.63DD105 pKa = 3.51EE106 pKa = 4.53AAEE109 pKa = 3.99RR110 pKa = 11.84TLFGLSVDD118 pKa = 3.66VGEE121 pKa = 4.95VGTPTYY127 pKa = 10.59DD128 pKa = 4.88RR129 pKa = 11.84PSTWDD134 pKa = 4.94DD135 pKa = 3.4EE136 pKa = 5.4DD137 pKa = 5.12GDD139 pKa = 4.85CISDD143 pKa = 3.21RR144 pKa = 11.84HH145 pKa = 6.31EE146 pKa = 3.9ILIAQHH152 pKa = 6.63LEE154 pKa = 4.09GEE156 pKa = 4.51GAYY159 pKa = 9.52PLVMSSNGCFVEE171 pKa = 4.25TGRR174 pKa = 11.84WLDD177 pKa = 3.77PYY179 pKa = 11.63DD180 pKa = 3.92NIYY183 pKa = 10.47YY184 pKa = 10.42YY185 pKa = 10.65SASDD189 pKa = 3.5VQIDD193 pKa = 3.87HH194 pKa = 6.89LVALYY199 pKa = 9.47EE200 pKa = 4.2SWISGLGNLDD210 pKa = 3.28AALQRR215 pKa = 11.84RR216 pKa = 11.84FANTGSLEE224 pKa = 4.07EE225 pKa = 4.33GVLPEE230 pKa = 4.09TSHH233 pKa = 6.73NFLAVGASSNGEE245 pKa = 4.19KK246 pKa = 10.62GSSDD250 pKa = 3.11PTEE253 pKa = 3.54WMPRR257 pKa = 11.84NEE259 pKa = 4.73AYY261 pKa = 10.37HH262 pKa = 6.94CIYY265 pKa = 9.96LKK267 pKa = 10.59KK268 pKa = 9.62WVLTKK273 pKa = 10.58SQNGLLIDD281 pKa = 3.49QAEE284 pKa = 3.96FDD286 pKa = 4.64FIEE289 pKa = 5.43GRR291 pKa = 11.84AADD294 pKa = 4.72CGAEE298 pKa = 4.31PLPEE302 pKa = 4.7LPPNPP307 pKa = 4.09

Molecular weight: 33.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H3NXZ9|H3NXZ9_9GAMM Putative glycosyltransferase OS=gamma proteobacterium HIMB55 OX=745014 GN=OMB55_00004400 PE=4 SV=1
MM1 pKa = 7.96LMRR4 pKa = 11.84EE5 pKa = 3.94RR6 pKa = 11.84FALYY10 pKa = 10.06RR11 pKa = 11.84SIGLGQIFVNCIFWLFRR28 pKa = 11.84KK29 pKa = 9.15QRR31 pKa = 11.84PRR33 pKa = 11.84FLVHH37 pKa = 5.74FTSVVIKK44 pKa = 9.89PGNITFSRR52 pKa = 11.84DD53 pKa = 2.96VTTLKK58 pKa = 10.8SLAVSAGCYY67 pKa = 8.4FQANNGIHH75 pKa = 7.01LGRR78 pKa = 11.84RR79 pKa = 11.84CLFAPGVKK87 pKa = 9.51IISSNHH93 pKa = 6.69DD94 pKa = 3.04IYY96 pKa = 11.54SADD99 pKa = 3.94RR100 pKa = 11.84KK101 pKa = 10.61SVVADD106 pKa = 5.04GITIGNDD113 pKa = 2.54VWIGANSVILPGVNIADD130 pKa = 3.66RR131 pKa = 11.84VVIGAGSVVTKK142 pKa = 10.72SISTPGAVVGGNPARR157 pKa = 11.84LLHH160 pKa = 6.28

Molecular weight: 17.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2470

0

2470

835561

29

2208

338.3

36.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.443 ± 0.054

0.964 ± 0.016

6.151 ± 0.038

6.48 ± 0.037

3.838 ± 0.032

8.006 ± 0.042

2.026 ± 0.026

5.395 ± 0.036

3.492 ± 0.034

9.926 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.555 ± 0.024

3.348 ± 0.031

4.458 ± 0.032

3.525 ± 0.026

5.703 ± 0.041

6.752 ± 0.039

5.524 ± 0.032

7.471 ± 0.04

1.37 ± 0.018

2.574 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski