Amino acid dipepetide frequency for gamma proteobacterium HIMB55

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.38AlaAla: 11.38 ± 0.138
1.068AlaCys: 1.068 ± 0.037
6.28AlaAsp: 6.28 ± 0.095
6.958AlaGlu: 6.958 ± 0.111
3.868AlaPhe: 3.868 ± 0.079
8.296AlaGly: 8.296 ± 0.101
1.891AlaHis: 1.891 ± 0.055
5.997AlaIle: 5.997 ± 0.088
3.803AlaLys: 3.803 ± 0.087
11.23AlaLeu: 11.23 ± 0.144
3.116AlaMet: 3.116 ± 0.072
3.377AlaAsn: 3.377 ± 0.07
4.072AlaPro: 4.072 ± 0.074
3.678AlaGln: 3.678 ± 0.067
5.501AlaArg: 5.501 ± 0.091
7.041AlaSer: 7.041 ± 0.106
5.475AlaThr: 5.475 ± 0.093
7.558AlaVal: 7.558 ± 0.101
1.293AlaTrp: 1.293 ± 0.038
2.544AlaTyr: 2.544 ± 0.049
0.0AlaXaa: 0.0 ± 0.0
Cys
0.917CysAla: 0.917 ± 0.035
0.138CysCys: 0.138 ± 0.014
0.646CysAsp: 0.646 ± 0.028
0.695CysGlu: 0.695 ± 0.029
0.399CysPhe: 0.399 ± 0.021
0.925CysGly: 0.925 ± 0.038
0.281CysHis: 0.281 ± 0.02
0.488CysIle: 0.488 ± 0.024
0.269CysLys: 0.269 ± 0.018
0.89CysLeu: 0.89 ± 0.033
0.194CysMet: 0.194 ± 0.015
0.297CysAsn: 0.297 ± 0.02
0.489CysPro: 0.489 ± 0.028
0.327CysGln: 0.327 ± 0.019
0.512CysArg: 0.512 ± 0.025
0.68CysSer: 0.68 ± 0.024
0.454CysThr: 0.454 ± 0.024
0.689CysVal: 0.689 ± 0.031
0.107CysTrp: 0.107 ± 0.012
0.247CysTyr: 0.247 ± 0.019
0.0CysXaa: 0.0 ± 0.0
Asp
6.566AspAla: 6.566 ± 0.099
0.616AspCys: 0.616 ± 0.031
3.689AspAsp: 3.689 ± 0.099
4.346AspGlu: 4.346 ± 0.074
2.397AspPhe: 2.397 ± 0.05
4.908AspGly: 4.908 ± 0.099
1.235AspHis: 1.235 ± 0.045
3.616AspIle: 3.616 ± 0.078
1.885AspLys: 1.885 ± 0.048
5.921AspLeu: 5.921 ± 0.091
1.521AspMet: 1.521 ± 0.041
1.927AspAsn: 1.927 ± 0.06
2.985AspPro: 2.985 ± 0.065
2.073AspGln: 2.073 ± 0.049
3.553AspArg: 3.553 ± 0.063
3.541AspSer: 3.541 ± 0.068
3.192AspThr: 3.192 ± 0.065
4.614AspVal: 4.614 ± 0.078
1.046AspTrp: 1.046 ± 0.039
1.877AspTyr: 1.877 ± 0.053
0.0AspXaa: 0.0 ± 0.0
Glu
7.287GluAla: 7.287 ± 0.102
0.479GluCys: 0.479 ± 0.023
3.268GluAsp: 3.268 ± 0.063
3.868GluGlu: 3.868 ± 0.078
2.23GluPhe: 2.23 ± 0.063
4.837GluGly: 4.837 ± 0.076
1.406GluHis: 1.406 ± 0.045
3.57GluIle: 3.57 ± 0.07
2.372GluLys: 2.372 ± 0.063
6.622GluLeu: 6.622 ± 0.093
1.592GluMet: 1.592 ± 0.043
2.093GluAsn: 2.093 ± 0.052
2.517GluPro: 2.517 ± 0.061
2.929GluGln: 2.929 ± 0.06
4.553GluArg: 4.553 ± 0.085
4.194GluSer: 4.194 ± 0.071
3.827GluThr: 3.827 ± 0.059
4.867GluVal: 4.867 ± 0.088
0.871GluTrp: 0.871 ± 0.035
1.413GluTyr: 1.413 ± 0.039
0.0GluXaa: 0.0 ± 0.0
Phe
3.797PheAla: 3.797 ± 0.072
0.474PheCys: 0.474 ± 0.026
2.92PheAsp: 2.92 ± 0.063
2.69PheGlu: 2.69 ± 0.063
1.714PhePhe: 1.714 ± 0.058
3.349PheGly: 3.349 ± 0.066
0.711PheHis: 0.711 ± 0.03
2.012PheIle: 2.012 ± 0.049
1.275PheLys: 1.275 ± 0.044
3.243PheLeu: 3.243 ± 0.083
0.904PheMet: 0.904 ± 0.034
1.497PheAsn: 1.497 ± 0.046
1.558PhePro: 1.558 ± 0.046
1.07PheGln: 1.07 ± 0.034
1.726PheArg: 1.726 ± 0.046
2.848PheSer: 2.848 ± 0.054
2.14PheThr: 2.14 ± 0.055
2.781PheVal: 2.781 ± 0.058
0.568PheTrp: 0.568 ± 0.027
1.105PheTyr: 1.105 ± 0.038
0.0PheXaa: 0.0 ± 0.0
Gly
7.746GlyAla: 7.746 ± 0.104
0.858GlyCys: 0.858 ± 0.033
4.966GlyAsp: 4.966 ± 0.094
5.049GlyGlu: 5.049 ± 0.07
3.617GlyPhe: 3.617 ± 0.074
6.596GlyGly: 6.596 ± 0.122
1.698GlyHis: 1.698 ± 0.044
4.608GlyIle: 4.608 ± 0.078
3.102GlyLys: 3.102 ± 0.067
8.031GlyLeu: 8.031 ± 0.114
2.219GlyMet: 2.219 ± 0.057
2.572GlyAsn: 2.572 ± 0.064
2.535GlyPro: 2.535 ± 0.062
2.675GlyGln: 2.675 ± 0.057
4.37GlyArg: 4.37 ± 0.078
5.089GlySer: 5.089 ± 0.092
4.128GlyThr: 4.128 ± 0.074
6.465GlyVal: 6.465 ± 0.1
1.273GlyTrp: 1.273 ± 0.04
2.468GlyTyr: 2.468 ± 0.057
0.0GlyXaa: 0.0 ± 0.0
His
1.783HisAla: 1.783 ± 0.046
0.281HisCys: 0.281 ± 0.019
1.017HisAsp: 1.017 ± 0.039
1.138HisGlu: 1.138 ± 0.04
0.898HisPhe: 0.898 ± 0.034
1.745HisGly: 1.745 ± 0.04
0.574HisHis: 0.574 ± 0.032
1.071HisIle: 1.071 ± 0.033
0.658HisLys: 0.658 ± 0.032
2.033HisLeu: 2.033 ± 0.056
0.5HisMet: 0.5 ± 0.022
0.649HisAsn: 0.649 ± 0.027
1.232HisPro: 1.232 ± 0.04
0.759HisGln: 0.759 ± 0.033
1.336HisArg: 1.336 ± 0.038
1.232HisSer: 1.232 ± 0.041
1.02HisThr: 1.02 ± 0.034
1.296HisVal: 1.296 ± 0.042
0.346HisTrp: 0.346 ± 0.02
0.697HisTyr: 0.697 ± 0.03
0.0HisXaa: 0.0 ± 0.0
Ile
6.316IleAla: 6.316 ± 0.09
0.561IleCys: 0.561 ± 0.026
4.229IleAsp: 4.229 ± 0.073
4.121IleGlu: 4.121 ± 0.077
1.828IlePhe: 1.828 ± 0.047
4.399IleGly: 4.399 ± 0.089
1.04IleHis: 1.04 ± 0.035
2.607IleIle: 2.607 ± 0.068
1.989IleLys: 1.989 ± 0.045
4.049IleLeu: 4.049 ± 0.082
1.064IleMet: 1.064 ± 0.038
2.102IleAsn: 2.102 ± 0.053
2.614IlePro: 2.614 ± 0.057
1.642IleGln: 1.642 ± 0.049
2.921IleArg: 2.921 ± 0.062
3.686IleSer: 3.686 ± 0.074
3.174IleThr: 3.174 ± 0.059
3.703IleVal: 3.703 ± 0.07
0.597IleTrp: 0.597 ± 0.027
1.31IleTyr: 1.31 ± 0.037
0.0IleXaa: 0.0 ± 0.0
Lys
3.949LysAla: 3.949 ± 0.074
0.238LysCys: 0.238 ± 0.018
1.772LysAsp: 1.772 ± 0.046
1.818LysGlu: 1.818 ± 0.06
1.069LysPhe: 1.069 ± 0.037
2.516LysGly: 2.516 ± 0.059
0.783LysHis: 0.783 ± 0.032
1.662LysIle: 1.662 ± 0.039
1.363LysLys: 1.363 ± 0.046
3.532LysLeu: 3.532 ± 0.075
0.822LysMet: 0.822 ± 0.032
1.157LysAsn: 1.157 ± 0.036
1.958LysPro: 1.958 ± 0.049
1.482LysGln: 1.482 ± 0.045
2.589LysArg: 2.589 ± 0.061
2.333LysSer: 2.333 ± 0.062
2.085LysThr: 2.085 ± 0.048
2.579LysVal: 2.579 ± 0.059
0.397LysTrp: 0.397 ± 0.02
0.814LysTyr: 0.814 ± 0.034
0.0LysXaa: 0.0 ± 0.0
Leu
10.784LeuAla: 10.784 ± 0.136
0.949LeuCys: 0.949 ± 0.037
6.037LeuAsp: 6.037 ± 0.093
6.458LeuGlu: 6.458 ± 0.092
3.678LeuPhe: 3.678 ± 0.085
7.779LeuGly: 7.779 ± 0.116
1.792LeuHis: 1.792 ± 0.05
5.295LeuIle: 5.295 ± 0.096
3.774LeuLys: 3.774 ± 0.082
9.562LeuLeu: 9.562 ± 0.136
2.537LeuMet: 2.537 ± 0.06
3.418LeuAsn: 3.418 ± 0.056
4.891LeuPro: 4.891 ± 0.074
3.063LeuGln: 3.063 ± 0.065
5.298LeuArg: 5.298 ± 0.092
7.731LeuSer: 7.731 ± 0.103
5.673LeuThr: 5.673 ± 0.081
7.11LeuVal: 7.11 ± 0.104
1.273LeuTrp: 1.273 ± 0.045
2.154LeuTyr: 2.154 ± 0.047
0.0LeuXaa: 0.0 ± 0.0
Met
2.902MetAla: 2.902 ± 0.059
0.19MetCys: 0.19 ± 0.015
1.3MetAsp: 1.3 ± 0.038
1.178MetGlu: 1.178 ± 0.041
0.743MetPhe: 0.743 ± 0.033
2.221MetGly: 2.221 ± 0.055
0.5MetHis: 0.5 ± 0.027
1.214MetIle: 1.214 ± 0.043
1.041MetLys: 1.041 ± 0.035
2.359MetLeu: 2.359 ± 0.053
0.737MetMet: 0.737 ± 0.035
0.973MetAsn: 0.973 ± 0.035
1.307MetPro: 1.307 ± 0.044
0.922MetGln: 0.922 ± 0.037
1.559MetArg: 1.559 ± 0.04
2.105MetSer: 2.105 ± 0.045
1.835MetThr: 1.835 ± 0.05
1.741MetVal: 1.741 ± 0.044
0.249MetTrp: 0.249 ± 0.016
0.473MetTyr: 0.473 ± 0.028
0.0MetXaa: 0.0 ± 0.0
Asn
3.594AsnAla: 3.594 ± 0.067
0.366AsnCys: 0.366 ± 0.022
1.871AsnAsp: 1.871 ± 0.046
2.09AsnGlu: 2.09 ± 0.047
1.27AsnPhe: 1.27 ± 0.04
2.687AsnGly: 2.687 ± 0.068
0.674AsnHis: 0.674 ± 0.03
1.844AsnIle: 1.844 ± 0.046
1.072AsnLys: 1.072 ± 0.036
2.985AsnLeu: 2.985 ± 0.059
0.766AsnMet: 0.766 ± 0.028
1.239AsnAsn: 1.239 ± 0.042
2.011AsnPro: 2.011 ± 0.051
1.235AsnGln: 1.235 ± 0.044
1.968AsnArg: 1.968 ± 0.059
1.86AsnSer: 1.86 ± 0.046
1.995AsnThr: 1.995 ± 0.051
2.463AsnVal: 2.463 ± 0.053
0.525AsnTrp: 0.525 ± 0.025
0.962AsnTyr: 0.962 ± 0.035
0.0AsnXaa: 0.0 ± 0.0
Pro
4.061ProAla: 4.061 ± 0.072
0.314ProCys: 0.314 ± 0.018
3.026ProAsp: 3.026 ± 0.063
3.8ProGlu: 3.8 ± 0.079
1.72ProPhe: 1.72 ± 0.051
3.454ProGly: 3.454 ± 0.068
0.928ProHis: 0.928 ± 0.036
2.394ProIle: 2.394 ± 0.048
1.766ProLys: 1.766 ± 0.047
4.253ProLeu: 4.253 ± 0.071
1.185ProMet: 1.185 ± 0.038
1.752ProAsn: 1.752 ± 0.046
1.594ProPro: 1.594 ± 0.05
1.437ProGln: 1.437 ± 0.045
2.039ProArg: 2.039 ± 0.058
3.162ProSer: 3.162 ± 0.05
2.47ProThr: 2.47 ± 0.062
3.461ProVal: 3.461 ± 0.066
0.583ProTrp: 0.583 ± 0.027
1.182ProTyr: 1.182 ± 0.038
0.0ProXaa: 0.0 ± 0.0
Gln
3.633GlnAla: 3.633 ± 0.071
0.316GlnCys: 0.316 ± 0.021
1.5GlnAsp: 1.5 ± 0.043
1.739GlnGlu: 1.739 ± 0.047
1.379GlnPhe: 1.379 ± 0.042
2.562GlnGly: 2.562 ± 0.052
0.782GlnHis: 0.782 ± 0.03
1.912GlnIle: 1.912 ± 0.041
1.214GlnLys: 1.214 ± 0.039
4.068GlnLeu: 4.068 ± 0.083
1.024GlnMet: 1.024 ± 0.034
1.018GlnAsn: 1.018 ± 0.038
1.574GlnPro: 1.574 ± 0.042
1.561GlnGln: 1.561 ± 0.049
2.633GlnArg: 2.633 ± 0.06
2.435GlnSer: 2.435 ± 0.06
1.929GlnThr: 1.929 ± 0.043
2.495GlnVal: 2.495 ± 0.062
0.591GlnTrp: 0.591 ± 0.029
0.883GlnTyr: 0.883 ± 0.033
0.0GlnXaa: 0.0 ± 0.0
Arg
5.534ArgAla: 5.534 ± 0.08
0.536ArgCys: 0.536 ± 0.028
3.72ArgAsp: 3.72 ± 0.071
4.018ArgGlu: 4.018 ± 0.067
2.469ArgPhe: 2.469 ± 0.05
4.051ArgGly: 4.051 ± 0.08
1.273ArgHis: 1.273 ± 0.036
3.049ArgIle: 3.049 ± 0.058
1.965ArgLys: 1.965 ± 0.045
6.071ArgLeu: 6.071 ± 0.104
1.468ArgMet: 1.468 ± 0.043
1.826ArgAsn: 1.826 ± 0.052
2.246ArgPro: 2.246 ± 0.057
2.255ArgGln: 2.255 ± 0.057
3.411ArgArg: 3.411 ± 0.071
3.498ArgSer: 3.498 ± 0.069
2.576ArgThr: 2.576 ± 0.055
4.417ArgVal: 4.417 ± 0.074
0.899ArgTrp: 0.899 ± 0.035
1.751ArgTyr: 1.751 ± 0.039
0.0ArgXaa: 0.0 ± 0.0
Ser
6.933SerAla: 6.933 ± 0.09
0.535SerCys: 0.535 ± 0.026
4.514SerAsp: 4.514 ± 0.082
4.571SerGlu: 4.571 ± 0.074
2.503SerPhe: 2.503 ± 0.055
6.004SerGly: 6.004 ± 0.097
1.36SerHis: 1.36 ± 0.043
3.357SerIle: 3.357 ± 0.06
2.195SerLys: 2.195 ± 0.051
6.861SerLeu: 6.861 ± 0.093
1.744SerMet: 1.744 ± 0.051
2.079SerAsn: 2.079 ± 0.049
2.935SerPro: 2.935 ± 0.058
2.516SerGln: 2.516 ± 0.058
3.625SerArg: 3.625 ± 0.071
4.514SerSer: 4.514 ± 0.091
3.587SerThr: 3.587 ± 0.072
5.146SerVal: 5.146 ± 0.079
0.882SerTrp: 0.882 ± 0.028
1.656SerTyr: 1.656 ± 0.048
0.0SerXaa: 0.0 ± 0.0
Thr
5.708ThrAla: 5.708 ± 0.081
0.482ThrCys: 0.482 ± 0.026
3.417ThrAsp: 3.417 ± 0.076
3.246ThrGlu: 3.246 ± 0.067
1.948ThrPhe: 1.948 ± 0.049
4.873ThrGly: 4.873 ± 0.084
1.151ThrHis: 1.151 ± 0.037
2.866ThrIle: 2.866 ± 0.065
1.588ThrLys: 1.588 ± 0.046
6.232ThrLeu: 6.232 ± 0.09
1.198ThrMet: 1.198 ± 0.043
1.716ThrAsn: 1.716 ± 0.053
3.075ThrPro: 3.075 ± 0.056
1.903ThrGln: 1.903 ± 0.043
2.88ThrArg: 2.88 ± 0.053
3.541ThrSer: 3.541 ± 0.061
3.065ThrThr: 3.065 ± 0.065
4.247ThrVal: 4.247 ± 0.075
0.682ThrTrp: 0.682 ± 0.032
1.421ThrTyr: 1.421 ± 0.047
0.0ThrXaa: 0.0 ± 0.0
Val
8.008ValAla: 8.008 ± 0.12
0.768ValCys: 0.768 ± 0.031
5.024ValAsp: 5.024 ± 0.08
4.695ValGlu: 4.695 ± 0.075
3.004ValPhe: 3.004 ± 0.061
5.641ValGly: 5.641 ± 0.103
1.24ValHis: 1.24 ± 0.042
4.572ValIle: 4.572 ± 0.063
2.438ValLys: 2.438 ± 0.056
6.939ValLeu: 6.939 ± 0.103
2.019ValMet: 2.019 ± 0.056
2.593ValAsn: 2.593 ± 0.059
3.175ValPro: 3.175 ± 0.06
1.989ValGln: 1.989 ± 0.046
3.802ValArg: 3.802 ± 0.061
5.41ValSer: 5.41 ± 0.086
4.614ValThr: 4.614 ± 0.092
6.247ValVal: 6.247 ± 0.105
0.849ValTrp: 0.849 ± 0.033
1.679ValTyr: 1.679 ± 0.042
0.0ValXaa: 0.0 ± 0.0
Trp
1.04TrpAla: 1.04 ± 0.036
0.169TrpCys: 0.169 ± 0.014
0.792TrpAsp: 0.792 ± 0.031
0.744TrpGlu: 0.744 ± 0.033
0.57TrpPhe: 0.57 ± 0.028
0.986TrpGly: 0.986 ± 0.037
0.333TrpHis: 0.333 ± 0.022
0.695TrpIle: 0.695 ± 0.033
0.36TrpLys: 0.36 ± 0.018
1.702TrpLeu: 1.702 ± 0.05
0.405TrpMet: 0.405 ± 0.022
0.403TrpAsn: 0.403 ± 0.021
0.531TrpPro: 0.531 ± 0.025
0.687TrpGln: 0.687 ± 0.03
0.968TrpArg: 0.968 ± 0.034
0.978TrpSer: 0.978 ± 0.039
0.661TrpThr: 0.661 ± 0.029
1.053TrpVal: 1.053 ± 0.04
0.247TrpTrp: 0.247 ± 0.018
0.378TrpTyr: 0.378 ± 0.024
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.487TyrAla: 2.487 ± 0.055
0.305TyrCys: 0.305 ± 0.02
1.519TyrAsp: 1.519 ± 0.041
1.575TyrGlu: 1.575 ± 0.049
1.082TyrPhe: 1.082 ± 0.039
2.232TyrGly: 2.232 ± 0.05
0.613TyrHis: 0.613 ± 0.034
1.123TyrIle: 1.123 ± 0.036
0.787TyrLys: 0.787 ± 0.033
2.675TyrLeu: 2.675 ± 0.063
0.533TyrMet: 0.533 ± 0.027
0.786TyrAsn: 0.786 ± 0.034
1.283TyrPro: 1.283 ± 0.042
1.05TyrGln: 1.05 ± 0.034
1.792TyrArg: 1.792 ± 0.051
1.638TyrSer: 1.638 ± 0.048
1.342TyrThr: 1.342 ± 0.046
1.77TyrVal: 1.77 ± 0.047
0.424TyrTrp: 0.424 ± 0.023
0.724TyrTyr: 0.724 ± 0.036
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2470 proteins (835561 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski