Proteome-pI 2.0 - Proteome Isoelectric Point Database

Bifidobacterium angulatum DSM 20098 = JCM 7096

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium; Bifidobacterium angulatum

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 1724 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|C4FGK5|C4FGK5_9BIFI Lysine--tRNA ligase OS=Bifidobacterium angulatum DSM 20098 = JCM 7096 OX=518635 GN=lysS PE=3 SV=1

MM1 pKa = 7.81SEE3 pKa = 4.12AQDD6 pKa = 3.78MQPLNLDD13 pKa = 3.63VPDD16 pKa = 4.2HH17 pKa = 6.95LEE19 pKa = 3.88YY20 pKa = 10.33TQDD23 pKa = 3.67HH24 pKa = 5.62VWVDD28 pKa = 3.37TSGDD32 pKa = 3.56LAVIGITEE40 pKa = 4.05YY41 pKa = 11.01AAEE44 pKa = 3.96QLGEE48 pKa = 4.25LVYY51 pKa = 11.06VDD53 pKa = 4.83LPEE56 pKa = 5.36PGTQIEE62 pKa = 4.4AGDD65 pKa = 3.69EE66 pKa = 4.25VFEE69 pKa = 4.83LEE71 pKa = 4.02SSKK74 pKa = 11.11AVEE77 pKa = 3.9PLIAPVSGTIRR88 pKa = 11.84YY89 pKa = 8.31VNQAVSDD96 pKa = 4.09DD97 pKa = 3.91PSVVNGDD104 pKa = 3.7PYY106 pKa = 11.6GEE108 pKa = 4.16GWLLKK113 pKa = 10.77VEE115 pKa = 4.84LDD117 pKa = 4.31DD118 pKa = 6.62DD119 pKa = 4.53EE120 pKa = 6.84PDD122 pKa = 3.59LLSAAEE128 pKa = 3.95YY129 pKa = 10.5LKK131 pKa = 10.87AVRR134 pKa = 4.84

Molecular weight: 14.65 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|C4FD73|C4FD73_9BIFI DNA gyrase subunit B OS=Bifidobacterium angulatum DSM 20098 = JCM 7096 OX=518635 GN=gyrB PE=3 SV=1

MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84HH13 pKa = 4.82MKK15 pKa = 9.64HH16 pKa = 5.7GFRR19 pKa = 11.84LRR21 pKa = 11.84MRR23 pKa = 11.84TRR25 pKa = 11.84SGRR28 pKa = 11.84AVINRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.04GRR39 pKa = 11.84KK40 pKa = 8.57NLSAA44 pKa = 4.67

Molecular weight: 5.4 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
1724	0	1724	587324	32	1972	340.7	37.2

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
10.914 ± 0.07	1.03 ± 0.019	6.31 ± 0.051	5.702 ± 0.057	3.45 ± 0.041	8.037 ± 0.047	2.226 ± 0.028	5.456 ± 0.045	4.122 ± 0.056	8.682 ± 0.063

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
2.801 ± 0.027	3.514 ± 0.045	4.431 ± 0.038	3.495 ± 0.04	5.802 ± 0.055	6.101 ± 0.055	6.024 ± 0.052	7.776 ± 0.049	1.356 ± 0.023	2.773 ± 0.035

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski