Corynebacterium pyruviciproducens ATCC BAA-1742
Average proteome isoelectric point is 5.99
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2443 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|S2ZUQ6|S2ZUQ6_9CORY Sigma-70 family RNA polymerase sigma factor OS=Corynebacterium pyruviciproducens ATCC BAA-1742 OX=1125779 GN=HMPREF1219_02157 PE=3 SV=1
MM1 pKa = 7.32 LKK3 pKa = 10.3 AWGQQPVTDD12 pKa = 3.92 SAGMGTMDD20 pKa = 3.93 EE21 pKa = 4.45 MGGMDD26 pKa = 4.1 HH27 pKa = 7.75 GEE29 pKa = 4.04 MGGMSGMMSQEE40 pKa = 3.97 DD41 pKa = 3.63 MTALEE46 pKa = 4.45 EE47 pKa = 4.32 AQGTQAACLYY57 pKa = 9.23 LEE59 pKa = 4.39 QMTAHH64 pKa = 6.81 HH65 pKa = 6.67 EE66 pKa = 4.37 GAVDD70 pKa = 3.72 MACDD74 pKa = 3.75 EE75 pKa = 4.47 VADD78 pKa = 4.26 GQNRR82 pKa = 11.84 HH83 pKa = 6.53 AITLAEE89 pKa = 4.09 QIINDD94 pKa = 3.7 QEE96 pKa = 4.37 AEE98 pKa = 4.04 IAQMLTDD105 pKa = 4.08 LL106 pKa = 4.82
Molecular weight: 11.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.763
IPC2_protein 3.846
IPC_protein 3.77
Toseland 3.592
ProMoST 3.935
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.668
Rodwell 3.617
Grimsley 3.503
Solomon 3.732
Lehninger 3.694
Nozaki 3.884
DTASelect 4.037
Thurlkill 3.643
EMBOSS 3.681
Sillero 3.897
Patrickios 1.863
IPC_peptide 3.732
IPC2_peptide 3.872
IPC2.peptide.svr19 3.8
Protein with the highest isoelectric point:
>tr|S2ZDC9|S2ZDC9_9CORY Uncharacterized protein OS=Corynebacterium pyruviciproducens ATCC BAA-1742 OX=1125779 GN=HMPREF1219_02400 PE=4 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.38 KK7 pKa = 8.42 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.06 RR11 pKa = 11.84 MSKK14 pKa = 9.63 KK15 pKa = 9.39 KK16 pKa = 9.04 HH17 pKa = 5.39 RR18 pKa = 11.84 KK19 pKa = 5.37 MLRR22 pKa = 11.84 RR23 pKa = 11.84 TRR25 pKa = 11.84 VQRR28 pKa = 11.84 RR29 pKa = 11.84 KK30 pKa = 10.11 LGKK33 pKa = 9.87
Molecular weight: 4.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.507
IPC2_protein 11.184
IPC_protein 12.749
Toseland 12.925
ProMoST 13.408
Dawson 12.925
Bjellqvist 12.91
Wikipedia 13.393
Rodwell 12.735
Grimsley 12.954
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.457
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.111
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2443
0
2443
781148
23
2666
319.7
34.68
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.905 ± 0.061
0.844 ± 0.017
6.024 ± 0.042
6.268 ± 0.047
3.414 ± 0.033
8.367 ± 0.044
2.202 ± 0.026
5.131 ± 0.037
3.794 ± 0.041
9.231 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.199 ± 0.023
2.916 ± 0.03
5.182 ± 0.041
3.089 ± 0.024
5.808 ± 0.05
5.89 ± 0.036
6.658 ± 0.052
8.327 ± 0.041
1.346 ± 0.021
2.404 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here