Flavihumibacter solisilvae
Average proteome isoelectric point is 6.76
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4215 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0C1IH91|A0A0C1IH91_9BACT 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Flavihumibacter solisilvae OX=1349421 GN=metE PE=3 SV=1
MM1 pKa = 7.22 VAPTDD6 pKa = 3.4 TMGRR10 pKa = 11.84 GVFTSEE16 pKa = 4.63 SIPANTLVEE25 pKa = 4.23 ISPVIVLSDD34 pKa = 3.95 GDD36 pKa = 4.43 RR37 pKa = 11.84 KK38 pKa = 10.73 LLDD41 pKa = 3.14 QTKK44 pKa = 8.64 LHH46 pKa = 7.17 DD47 pKa = 5.28 YY48 pKa = 9.92 IFEE51 pKa = 4.28 WGEE54 pKa = 3.93 DD55 pKa = 3.72 LQDD58 pKa = 3.18 CCMALGYY65 pKa = 10.2 IPLYY69 pKa = 8.51 NHH71 pKa = 6.92 SYY73 pKa = 10.91 SSNCEE78 pKa = 3.62 YY79 pKa = 11.14 EE80 pKa = 4.08 MDD82 pKa = 4.21 FDD84 pKa = 3.75 AHH86 pKa = 6.83 LISVKK91 pKa = 7.45 TVRR94 pKa = 11.84 DD95 pKa = 3.45 IEE97 pKa = 4.5 AGEE100 pKa = 4.05 EE101 pKa = 4.06 LFINYY106 pKa = 9.94 NGDD109 pKa = 3.28 WDD111 pKa = 4.16 NNAPLWFDD119 pKa = 3.51 ALL121 pKa = 3.86
Molecular weight: 13.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.796
IPC2_protein 3.961
IPC_protein 3.923
Toseland 3.706
ProMoST 4.075
Dawson 3.91
Bjellqvist 4.062
Wikipedia 3.834
Rodwell 3.745
Grimsley 3.63
Solomon 3.897
Lehninger 3.846
Nozaki 4.024
DTASelect 4.24
Thurlkill 3.77
EMBOSS 3.846
Sillero 4.037
Patrickios 0.693
IPC_peptide 3.897
IPC2_peptide 4.012
IPC2.peptide.svr19 3.925
Protein with the highest isoelectric point:
>tr|A0A0C1L8J3|A0A0C1L8J3_9BACT Uncharacterized protein OS=Flavihumibacter solisilvae OX=1349421 GN=OI18_01725 PE=4 SV=1
MM1 pKa = 7.85 SIPPSPFRR9 pKa = 11.84 DD10 pKa = 3.38 LQKK13 pKa = 10.6 LYY15 pKa = 10.85 RR16 pKa = 11.84 IFLYY20 pKa = 10.22 SHH22 pKa = 7.02 IGSVIISVLIVSLSLMKK39 pKa = 10.45 PFAAVTLDD47 pKa = 3.54 RR48 pKa = 11.84 LLQVIAVLYY57 pKa = 10.4 SFLAVIVGLQLFRR70 pKa = 11.84 RR71 pKa = 11.84 RR72 pKa = 11.84 VNAIRR77 pKa = 11.84 EE78 pKa = 4.3 NNQSVRR84 pKa = 11.84 DD85 pKa = 3.7 KK86 pKa = 10.92 LAQFRR91 pKa = 11.84 SGCIVQWALLTGAGFFCSISYY112 pKa = 10.39 VITGNWAFPLLAIMLMAIFGGLNPFRR138 pKa = 11.84 QKK140 pKa = 11.13 VMLQLRR146 pKa = 11.84 LSEE149 pKa = 3.98 QDD151 pKa = 3.4 VAGMM155 pKa = 4.24
Molecular weight: 17.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.337
IPC2_protein 9.765
IPC_protein 10.628
Toseland 10.511
ProMoST 10.277
Dawson 10.672
Bjellqvist 10.423
Wikipedia 10.906
Rodwell 10.818
Grimsley 10.745
Solomon 10.76
Lehninger 10.716
Nozaki 10.511
DTASelect 10.409
Thurlkill 10.54
EMBOSS 10.921
Sillero 10.599
Patrickios 10.57
IPC_peptide 10.76
IPC2_peptide 9.575
IPC2.peptide.svr19 8.384
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4215
0
4215
1510737
34
2895
358.4
40.14
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.717 ± 0.038
0.834 ± 0.011
5.261 ± 0.026
5.794 ± 0.036
4.847 ± 0.028
7.233 ± 0.032
1.961 ± 0.019
6.673 ± 0.031
6.066 ± 0.028
9.454 ± 0.038
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.452 ± 0.019
5.108 ± 0.032
4.141 ± 0.022
3.74 ± 0.024
4.784 ± 0.023
6.44 ± 0.03
5.621 ± 0.033
6.613 ± 0.027
1.377 ± 0.016
3.884 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here