Janibacter limosus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria;

Average proteome isoelectric point is 5.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3282 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P6MSA9|A0A4P6MSA9_9MICO Class I SAM-dependent methyltransferase OS=Janibacter limosus OX=53458 GN=EXU32_09430 PE=4 SV=1
MM1 pKa = 7.82ASTLNPYY8 pKa = 11.03LNFDD12 pKa = 3.69ANARR16 pKa = 11.84EE17 pKa = 3.99AMEE20 pKa = 5.29FYY22 pKa = 10.83QSVLGGDD29 pKa = 3.69LVINTFGEE37 pKa = 4.27YY38 pKa = 9.68MPDD41 pKa = 3.24AGDD44 pKa = 3.51IADD47 pKa = 4.05QVMHH51 pKa = 7.25ASLQTEE57 pKa = 4.03AGYY60 pKa = 8.84TIFASDD66 pKa = 4.6TPPGMQSTPGTSITVSISGDD86 pKa = 3.1EE87 pKa = 4.28TEE89 pKa = 4.43RR90 pKa = 11.84LRR92 pKa = 11.84GYY94 pKa = 9.37WAGLTEE100 pKa = 4.7GGTIEE105 pKa = 4.95MPLEE109 pKa = 3.75MQMWGDD115 pKa = 3.85EE116 pKa = 4.34YY117 pKa = 11.6GSFTDD122 pKa = 4.05RR123 pKa = 11.84FGIPWMVNIAGPGQPGG139 pKa = 2.89

Molecular weight: 15.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P6MZI5|A0A4P6MZI5_9MICO OmpA family protein OS=Janibacter limosus OX=53458 GN=EXU32_15685 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84SILANRR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.73GRR40 pKa = 11.84GKK42 pKa = 10.27ISAA45 pKa = 4.02

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3282

0

3282

1067424

32

1648

325.2

34.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.776 ± 0.053

0.674 ± 0.011

6.529 ± 0.035

5.782 ± 0.044

2.614 ± 0.027

9.309 ± 0.043

2.331 ± 0.025

3.938 ± 0.03

2.096 ± 0.036

10.153 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.993 ± 0.017

1.594 ± 0.021

5.52 ± 0.032

2.921 ± 0.023

7.47 ± 0.046

5.506 ± 0.03

6.327 ± 0.032

9.19 ± 0.043

1.524 ± 0.02

1.753 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski