Salmonella phage SE21

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Lederbergvirus; unclassified Lederbergvirus

Average proteome isoelectric point is 6.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 66 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C0CBN8|A0A5C0CBN8_9CAUD CII protein OS=Salmonella phage SE21 OX=2592200 PE=4 SV=1
MM1 pKa = 7.71LSPTQIMQYY10 pKa = 9.75QKK12 pKa = 10.94EE13 pKa = 4.53SVDD16 pKa = 4.19RR17 pKa = 11.84ALTCANCGQKK27 pKa = 10.32LHH29 pKa = 5.86VLEE32 pKa = 4.29VHH34 pKa = 5.94VCEE37 pKa = 4.68ACCSEE42 pKa = 5.43LMSDD46 pKa = 4.48PNSAMYY52 pKa = 10.4EE53 pKa = 4.1EE54 pKa = 5.48EE55 pKa = 5.11DD56 pKa = 5.44DD57 pKa = 4.54EE58 pKa = 7.52DD59 pKa = 5.12DD60 pKa = 3.93FAPGKK65 pKa = 10.34EE66 pKa = 4.16DD67 pKa = 3.16

Molecular weight: 7.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C0CC01|A0A5C0CC01_9CAUD Protein Kil OS=Salmonella phage SE21 OX=2592200 PE=4 SV=1
MM1 pKa = 7.92IIRR4 pKa = 11.84HH5 pKa = 5.61RR6 pKa = 11.84FKK8 pKa = 10.61PINDD12 pKa = 3.38IPRR15 pKa = 11.84EE16 pKa = 3.78EE17 pKa = 3.91MLARR21 pKa = 11.84KK22 pKa = 8.92SFPSVNEE29 pKa = 3.83NKK31 pKa = 10.35YY32 pKa = 10.3LNAMLRR38 pKa = 11.84SGKK41 pKa = 9.99KK42 pKa = 9.6

Molecular weight: 5.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

66

0

66

11820

41

725

179.1

20.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.756 ± 0.445

1.227 ± 0.139

6.1 ± 0.235

6.946 ± 0.352

3.511 ± 0.236

6.633 ± 0.378

1.76 ± 0.165

6.286 ± 0.366

6.836 ± 0.302

8.003 ± 0.223

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.995 ± 0.164

4.679 ± 0.217

3.917 ± 0.187

4.019 ± 0.321

5.998 ± 0.299

6.21 ± 0.259

5.0 ± 0.286

6.007 ± 0.246

1.726 ± 0.154

3.393 ± 0.206
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski