Amino acid dipepetide frequency for Salmonella phage SE21

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.292AlaAla: 8.292 ± 1.343
1.1AlaCys: 1.1 ± 0.357
5.161AlaAsp: 5.161 ± 0.835
7.361AlaGlu: 7.361 ± 0.972
3.215AlaPhe: 3.215 ± 0.593
6.346AlaGly: 6.346 ± 0.788
0.931AlaHis: 0.931 ± 0.289
5.584AlaIle: 5.584 ± 0.82
4.738AlaLys: 4.738 ± 0.708
6.515AlaLeu: 6.515 ± 0.944
3.807AlaMet: 3.807 ± 0.653
5.246AlaAsn: 5.246 ± 0.827
2.454AlaPro: 2.454 ± 0.481
3.046AlaGln: 3.046 ± 0.534
4.823AlaArg: 4.823 ± 0.778
4.569AlaSer: 4.569 ± 0.6
4.738AlaThr: 4.738 ± 0.76
5.161AlaVal: 5.161 ± 0.591
1.438AlaTrp: 1.438 ± 0.387
3.046AlaTyr: 3.046 ± 0.535
0.0AlaXaa: 0.0 ± 0.0
Cys
0.761CysAla: 0.761 ± 0.288
0.338CysCys: 0.338 ± 0.189
0.761CysAsp: 0.761 ± 0.265
0.761CysGlu: 0.761 ± 0.285
0.423CysPhe: 0.423 ± 0.204
0.931CysGly: 0.931 ± 0.339
0.254CysHis: 0.254 ± 0.122
0.592CysIle: 0.592 ± 0.237
0.846CysLys: 0.846 ± 0.32
0.338CysLeu: 0.338 ± 0.163
0.338CysMet: 0.338 ± 0.168
0.677CysAsn: 0.677 ± 0.295
0.592CysPro: 0.592 ± 0.26
0.423CysGln: 0.423 ± 0.243
1.269CysArg: 1.269 ± 0.341
1.1CysSer: 1.1 ± 0.324
0.423CysThr: 0.423 ± 0.195
0.846CysVal: 0.846 ± 0.254
0.169CysTrp: 0.169 ± 0.13
0.423CysTyr: 0.423 ± 0.186
0.0CysXaa: 0.0 ± 0.0
Asp
7.869AspAla: 7.869 ± 0.88
0.508AspCys: 0.508 ± 0.219
4.23AspAsp: 4.23 ± 0.622
4.484AspGlu: 4.484 ± 0.724
2.115AspPhe: 2.115 ± 0.475
5.5AspGly: 5.5 ± 0.8
0.931AspHis: 0.931 ± 0.314
3.977AspIle: 3.977 ± 0.5
3.723AspLys: 3.723 ± 0.501
4.484AspLeu: 4.484 ± 0.644
1.523AspMet: 1.523 ± 0.3
2.115AspAsn: 2.115 ± 0.462
1.692AspPro: 1.692 ± 0.389
1.354AspGln: 1.354 ± 0.353
2.284AspArg: 2.284 ± 0.377
3.807AspSer: 3.807 ± 0.478
2.2AspThr: 2.2 ± 0.367
4.23AspVal: 4.23 ± 0.582
1.269AspTrp: 1.269 ± 0.347
2.708AspTyr: 2.708 ± 0.511
0.0AspXaa: 0.0 ± 0.0
Glu
6.261GluAla: 6.261 ± 0.758
1.692GluCys: 1.692 ± 0.402
3.554GluAsp: 3.554 ± 0.563
4.738GluGlu: 4.738 ± 0.84
2.2GluPhe: 2.2 ± 0.621
2.792GluGly: 2.792 ± 0.401
1.438GluHis: 1.438 ± 0.371
3.977GluIle: 3.977 ± 0.598
4.23GluLys: 4.23 ± 0.657
6.769GluLeu: 6.769 ± 0.696
2.454GluMet: 2.454 ± 0.393
3.554GluAsn: 3.554 ± 0.585
2.961GluPro: 2.961 ± 0.568
3.977GluGln: 3.977 ± 0.62
4.654GluArg: 4.654 ± 0.624
3.723GluSer: 3.723 ± 0.46
3.131GluThr: 3.131 ± 0.453
3.469GluVal: 3.469 ± 0.508
1.777GluTrp: 1.777 ± 0.413
2.115GluTyr: 2.115 ± 0.458
0.0GluXaa: 0.0 ± 0.0
Phe
3.3PheAla: 3.3 ± 0.542
0.931PheCys: 0.931 ± 0.299
2.708PheAsp: 2.708 ± 0.463
1.946PheGlu: 1.946 ± 0.394
1.1PhePhe: 1.1 ± 0.431
2.2PheGly: 2.2 ± 0.398
0.423PheHis: 0.423 ± 0.16
3.046PheIle: 3.046 ± 0.619
1.946PheLys: 1.946 ± 0.416
2.031PheLeu: 2.031 ± 0.466
1.1PheMet: 1.1 ± 0.313
2.2PheAsn: 2.2 ± 0.445
1.269PhePro: 1.269 ± 0.289
0.931PheGln: 0.931 ± 0.33
1.608PheArg: 1.608 ± 0.379
3.215PheSer: 3.215 ± 0.544
2.031PheThr: 2.031 ± 0.388
1.269PheVal: 1.269 ± 0.334
0.761PheTrp: 0.761 ± 0.218
1.1PheTyr: 1.1 ± 0.327
0.0PheXaa: 0.0 ± 0.0
Gly
4.4GlyAla: 4.4 ± 0.687
0.508GlyCys: 0.508 ± 0.193
3.131GlyAsp: 3.131 ± 0.641
4.654GlyGlu: 4.654 ± 0.607
2.792GlyPhe: 2.792 ± 0.524
5.161GlyGly: 5.161 ± 0.804
1.354GlyHis: 1.354 ± 0.361
4.569GlyIle: 4.569 ± 0.631
5.584GlyLys: 5.584 ± 0.765
3.554GlyLeu: 3.554 ± 0.598
2.369GlyMet: 2.369 ± 0.534
3.215GlyAsn: 3.215 ± 0.459
0.931GlyPro: 0.931 ± 0.248
2.708GlyGln: 2.708 ± 0.522
4.4GlyArg: 4.4 ± 0.681
4.484GlySer: 4.484 ± 0.794
3.469GlyThr: 3.469 ± 0.594
5.161GlyVal: 5.161 ± 0.65
1.523GlyTrp: 1.523 ± 0.275
2.369GlyTyr: 2.369 ± 0.445
0.0GlyXaa: 0.0 ± 0.0
His
0.846HisAla: 0.846 ± 0.24
0.338HisCys: 0.338 ± 0.181
1.269HisAsp: 1.269 ± 0.314
1.692HisGlu: 1.692 ± 0.366
0.761HisPhe: 0.761 ± 0.221
0.931HisGly: 0.931 ± 0.301
0.592HisHis: 0.592 ± 0.198
0.761HisIle: 0.761 ± 0.202
1.1HisLys: 1.1 ± 0.347
1.608HisLeu: 1.608 ± 0.369
0.677HisMet: 0.677 ± 0.215
0.677HisAsn: 0.677 ± 0.227
1.185HisPro: 1.185 ± 0.358
0.677HisGln: 0.677 ± 0.241
1.269HisArg: 1.269 ± 0.318
0.592HisSer: 0.592 ± 0.226
0.677HisThr: 0.677 ± 0.214
1.1HisVal: 1.1 ± 0.31
0.169HisTrp: 0.169 ± 0.139
0.677HisTyr: 0.677 ± 0.225
0.0HisXaa: 0.0 ± 0.0
Ile
6.007IleAla: 6.007 ± 0.851
0.423IleCys: 0.423 ± 0.184
4.061IleAsp: 4.061 ± 0.544
4.146IleGlu: 4.146 ± 0.648
2.031IlePhe: 2.031 ± 0.445
4.738IleGly: 4.738 ± 0.752
1.185IleHis: 1.185 ± 0.319
4.23IleIle: 4.23 ± 0.941
4.23IleLys: 4.23 ± 0.664
3.384IleLeu: 3.384 ± 0.648
0.931IleMet: 0.931 ± 0.245
2.877IleAsn: 2.877 ± 0.568
3.469IlePro: 3.469 ± 0.479
1.354IleGln: 1.354 ± 0.296
3.977IleArg: 3.977 ± 0.46
5.5IleSer: 5.5 ± 0.886
4.23IleThr: 4.23 ± 0.653
3.384IleVal: 3.384 ± 0.608
0.508IleTrp: 0.508 ± 0.185
2.2IleTyr: 2.2 ± 0.442
0.0IleXaa: 0.0 ± 0.0
Lys
5.5LysAla: 5.5 ± 0.703
1.1LysCys: 1.1 ± 0.362
4.061LysAsp: 4.061 ± 0.613
4.484LysGlu: 4.484 ± 0.708
1.946LysPhe: 1.946 ± 0.413
3.977LysGly: 3.977 ± 0.475
0.677LysHis: 0.677 ± 0.265
3.215LysIle: 3.215 ± 0.462
5.161LysLys: 5.161 ± 0.738
6.43LysLeu: 6.43 ± 0.729
2.115LysMet: 2.115 ± 0.457
2.369LysAsn: 2.369 ± 0.486
3.892LysPro: 3.892 ± 0.54
3.469LysGln: 3.469 ± 0.648
5.5LysArg: 5.5 ± 0.67
3.807LysSer: 3.807 ± 0.664
4.315LysThr: 4.315 ± 0.526
3.554LysVal: 3.554 ± 0.597
0.761LysTrp: 0.761 ± 0.328
2.031LysTyr: 2.031 ± 0.413
0.0LysXaa: 0.0 ± 0.0
Leu
6.515LeuAla: 6.515 ± 0.818
0.423LeuCys: 0.423 ± 0.186
4.061LeuAsp: 4.061 ± 0.664
4.992LeuGlu: 4.992 ± 0.689
2.708LeuPhe: 2.708 ± 0.622
4.4LeuGly: 4.4 ± 0.71
1.692LeuHis: 1.692 ± 0.473
6.346LeuIle: 6.346 ± 0.921
6.769LeuLys: 6.769 ± 0.769
7.361LeuLeu: 7.361 ± 0.792
2.454LeuMet: 2.454 ± 0.36
4.484LeuAsn: 4.484 ± 0.675
3.046LeuPro: 3.046 ± 0.501
2.623LeuGln: 2.623 ± 0.509
4.4LeuArg: 4.4 ± 0.595
5.838LeuSer: 5.838 ± 0.657
4.061LeuThr: 4.061 ± 0.586
4.484LeuVal: 4.484 ± 0.452
1.1LeuTrp: 1.1 ± 0.361
2.284LeuTyr: 2.284 ± 0.405
0.0LeuXaa: 0.0 ± 0.0
Met
2.961MetAla: 2.961 ± 0.511
0.254MetCys: 0.254 ± 0.151
1.438MetAsp: 1.438 ± 0.375
1.608MetGlu: 1.608 ± 0.307
0.677MetPhe: 0.677 ± 0.31
1.777MetGly: 1.777 ± 0.363
0.085MetHis: 0.085 ± 0.09
1.438MetIle: 1.438 ± 0.368
2.538MetLys: 2.538 ± 0.507
2.284MetLeu: 2.284 ± 0.51
1.015MetMet: 1.015 ± 0.286
1.608MetAsn: 1.608 ± 0.373
1.185MetPro: 1.185 ± 0.295
1.269MetGln: 1.269 ± 0.353
2.369MetArg: 2.369 ± 0.537
2.2MetSer: 2.2 ± 0.502
2.115MetThr: 2.115 ± 0.441
1.861MetVal: 1.861 ± 0.379
0.254MetTrp: 0.254 ± 0.145
1.015MetTyr: 1.015 ± 0.276
0.0MetXaa: 0.0 ± 0.0
Asn
4.061AsnAla: 4.061 ± 0.759
0.338AsnCys: 0.338 ± 0.178
2.369AsnAsp: 2.369 ± 0.447
3.215AsnGlu: 3.215 ± 0.486
1.269AsnPhe: 1.269 ± 0.335
4.569AsnGly: 4.569 ± 0.621
1.015AsnHis: 1.015 ± 0.258
3.046AsnIle: 3.046 ± 0.562
2.708AsnLys: 2.708 ± 0.485
2.961AsnLeu: 2.961 ± 0.557
1.269AsnMet: 1.269 ± 0.393
1.946AsnAsn: 1.946 ± 0.401
2.538AsnPro: 2.538 ± 0.418
2.623AsnGln: 2.623 ± 0.549
1.692AsnArg: 1.692 ± 0.352
2.538AsnSer: 2.538 ± 0.384
3.215AsnThr: 3.215 ± 0.567
3.215AsnVal: 3.215 ± 0.69
0.508AsnTrp: 0.508 ± 0.209
1.692AsnTyr: 1.692 ± 0.345
0.0AsnXaa: 0.0 ± 0.0
Pro
2.538ProAla: 2.538 ± 0.446
0.169ProCys: 0.169 ± 0.116
3.3ProAsp: 3.3 ± 0.465
4.823ProGlu: 4.823 ± 0.697
1.523ProPhe: 1.523 ± 0.342
2.2ProGly: 2.2 ± 0.407
0.846ProHis: 0.846 ± 0.261
2.369ProIle: 2.369 ± 0.446
2.369ProLys: 2.369 ± 0.421
2.538ProLeu: 2.538 ± 0.508
1.015ProMet: 1.015 ± 0.359
1.692ProAsn: 1.692 ± 0.374
1.1ProPro: 1.1 ± 0.371
1.438ProGln: 1.438 ± 0.352
2.031ProArg: 2.031 ± 0.427
2.369ProSer: 2.369 ± 0.388
1.777ProThr: 1.777 ± 0.304
3.469ProVal: 3.469 ± 0.438
0.508ProTrp: 0.508 ± 0.231
1.1ProTyr: 1.1 ± 0.31
0.0ProXaa: 0.0 ± 0.0
Gln
3.723GlnAla: 3.723 ± 0.611
0.423GlnCys: 0.423 ± 0.172
2.623GlnAsp: 2.623 ± 0.458
2.031GlnGlu: 2.031 ± 0.461
0.931GlnPhe: 0.931 ± 0.243
2.792GlnGly: 2.792 ± 0.568
0.931GlnHis: 0.931 ± 0.247
3.046GlnIle: 3.046 ± 0.487
2.284GlnLys: 2.284 ± 0.606
3.807GlnLeu: 3.807 ± 0.547
1.1GlnMet: 1.1 ± 0.32
1.354GlnAsn: 1.354 ± 0.566
1.608GlnPro: 1.608 ± 0.349
1.861GlnGln: 1.861 ± 0.559
3.215GlnArg: 3.215 ± 0.43
2.115GlnSer: 2.115 ± 0.516
1.523GlnThr: 1.523 ± 0.4
1.777GlnVal: 1.777 ± 0.394
1.185GlnTrp: 1.185 ± 0.307
1.777GlnTyr: 1.777 ± 0.352
0.0GlnXaa: 0.0 ± 0.0
Arg
4.23ArgAla: 4.23 ± 0.466
0.592ArgCys: 0.592 ± 0.228
4.4ArgAsp: 4.4 ± 0.586
4.569ArgGlu: 4.569 ± 0.683
2.369ArgPhe: 2.369 ± 0.575
3.046ArgGly: 3.046 ± 0.393
1.523ArgHis: 1.523 ± 0.344
3.977ArgIle: 3.977 ± 0.565
4.992ArgLys: 4.992 ± 0.813
5.415ArgLeu: 5.415 ± 0.502
2.031ArgMet: 2.031 ± 0.421
2.877ArgAsn: 2.877 ± 0.597
1.692ArgPro: 1.692 ± 0.333
2.708ArgGln: 2.708 ± 0.506
3.723ArgArg: 3.723 ± 0.782
3.3ArgSer: 3.3 ± 0.514
2.031ArgThr: 2.031 ± 0.445
3.046ArgVal: 3.046 ± 0.555
1.185ArgTrp: 1.185 ± 0.426
2.284ArgTyr: 2.284 ± 0.453
0.0ArgXaa: 0.0 ± 0.0
Ser
4.569SerAla: 4.569 ± 0.879
1.015SerCys: 1.015 ± 0.347
3.469SerAsp: 3.469 ± 0.558
3.892SerGlu: 3.892 ± 0.576
2.538SerPhe: 2.538 ± 0.485
4.992SerGly: 4.992 ± 0.812
1.354SerHis: 1.354 ± 0.41
3.723SerIle: 3.723 ± 0.577
3.723SerLys: 3.723 ± 0.618
5.415SerLeu: 5.415 ± 0.582
1.861SerMet: 1.861 ± 0.338
2.877SerAsn: 2.877 ± 0.383
2.708SerPro: 2.708 ± 0.453
2.877SerGln: 2.877 ± 0.449
3.892SerArg: 3.892 ± 0.586
3.554SerSer: 3.554 ± 0.723
2.454SerThr: 2.454 ± 0.626
3.892SerVal: 3.892 ± 0.497
1.015SerTrp: 1.015 ± 0.295
2.284SerTyr: 2.284 ± 0.521
0.0SerXaa: 0.0 ± 0.0
Thr
5.923ThrAla: 5.923 ± 0.852
0.508ThrCys: 0.508 ± 0.199
2.877ThrAsp: 2.877 ± 0.644
2.708ThrGlu: 2.708 ± 0.52
1.269ThrPhe: 1.269 ± 0.333
3.723ThrGly: 3.723 ± 0.601
0.846ThrHis: 0.846 ± 0.27
3.131ThrIle: 3.131 ± 0.577
4.315ThrLys: 4.315 ± 0.664
4.061ThrLeu: 4.061 ± 0.61
1.015ThrMet: 1.015 ± 0.298
2.284ThrAsn: 2.284 ± 0.472
2.708ThrPro: 2.708 ± 0.458
2.115ThrGln: 2.115 ± 0.359
2.2ThrArg: 2.2 ± 0.403
3.215ThrSer: 3.215 ± 0.544
2.369ThrThr: 2.369 ± 0.407
2.623ThrVal: 2.623 ± 0.493
0.761ThrTrp: 0.761 ± 0.251
1.354ThrTyr: 1.354 ± 0.323
0.0ThrXaa: 0.0 ± 0.0
Val
6.092ValAla: 6.092 ± 0.703
0.508ValCys: 0.508 ± 0.247
3.554ValAsp: 3.554 ± 0.619
4.315ValGlu: 4.315 ± 0.457
2.792ValPhe: 2.792 ± 0.496
3.384ValGly: 3.384 ± 0.61
0.508ValHis: 0.508 ± 0.18
3.215ValIle: 3.215 ± 0.638
4.061ValLys: 4.061 ± 0.501
5.753ValLeu: 5.753 ± 0.563
1.608ValMet: 1.608 ± 0.408
3.3ValAsn: 3.3 ± 0.48
1.861ValPro: 1.861 ± 0.42
1.692ValGln: 1.692 ± 0.297
3.215ValArg: 3.215 ± 0.467
3.892ValSer: 3.892 ± 0.648
3.215ValThr: 3.215 ± 0.505
4.061ValVal: 4.061 ± 0.532
1.015ValTrp: 1.015 ± 0.295
2.031ValTyr: 2.031 ± 0.438
0.0ValXaa: 0.0 ± 0.0
Trp
0.761TrpAla: 0.761 ± 0.255
0.508TrpCys: 0.508 ± 0.269
1.185TrpAsp: 1.185 ± 0.267
1.015TrpGlu: 1.015 ± 0.321
1.015TrpPhe: 1.015 ± 0.293
0.677TrpGly: 0.677 ± 0.213
0.338TrpHis: 0.338 ± 0.169
0.761TrpIle: 0.761 ± 0.282
1.438TrpLys: 1.438 ± 0.375
2.538TrpLeu: 2.538 ± 0.562
0.592TrpMet: 0.592 ± 0.188
0.254TrpAsn: 0.254 ± 0.129
0.677TrpPro: 0.677 ± 0.207
0.931TrpGln: 0.931 ± 0.24
1.015TrpArg: 1.015 ± 0.32
0.931TrpSer: 0.931 ± 0.269
0.592TrpThr: 0.592 ± 0.22
1.185TrpVal: 1.185 ± 0.264
0.423TrpTrp: 0.423 ± 0.185
0.423TrpTyr: 0.423 ± 0.17
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.961TyrAla: 2.961 ± 0.511
0.592TyrCys: 0.592 ± 0.224
2.792TyrAsp: 2.792 ± 0.604
2.031TyrGlu: 2.031 ± 0.517
1.438TyrPhe: 1.438 ± 0.386
2.2TyrGly: 2.2 ± 0.462
0.677TyrHis: 0.677 ± 0.281
1.861TyrIle: 1.861 ± 0.599
1.608TyrLys: 1.608 ± 0.379
2.792TyrLeu: 2.792 ± 0.373
0.592TyrMet: 0.592 ± 0.234
1.185TyrAsn: 1.185 ± 0.4
1.608TyrPro: 1.608 ± 0.508
2.115TyrGln: 2.115 ± 0.448
2.454TyrArg: 2.454 ± 0.493
1.354TyrSer: 1.354 ± 0.363
1.438TyrThr: 1.438 ± 0.262
2.284TyrVal: 2.284 ± 0.429
0.931TyrTrp: 0.931 ± 0.317
1.015TyrTyr: 1.015 ± 0.318
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 66 proteins (11820 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski