Broad bean wilt virus 1 (strain Spinach/United States/ATCC PV-132/1963) (BBWV-1)
Average proteome isoelectric point is 6.66
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q76L39-2|POL2-2_BBWVS Isoform of Q76L39 Isoform RNA2 polyprotein 104kDa of RNA2 polyprotein OS=Broad bean wilt virus 1 (strain Spinach/United States/ATCC PV-132/1963) OX=649895 PE=4 SV=1
MM1 pKa = 7.92 DD2 pKa = 4.99 SEE4 pKa = 4.66 TIDD7 pKa = 3.4 MCVKK11 pKa = 10.2 FLKK14 pKa = 10.34 ISFGLQSLKK23 pKa = 11.05 NLVKK27 pKa = 10.35 EE28 pKa = 3.99 LFGGSEE34 pKa = 4.37 LEE36 pKa = 3.99 KK37 pKa = 10.5 LAYY40 pKa = 9.11 VHH42 pKa = 7.15 AAFFHH47 pKa = 5.96 ANEE50 pKa = 4.4 MAIHH54 pKa = 6.64 WNADD58 pKa = 3.81 LPWEE62 pKa = 4.16 EE63 pKa = 4.15 VMSSKK68 pKa = 10.54 RR69 pKa = 11.84 IKK71 pKa = 10.44 EE72 pKa = 3.61 RR73 pKa = 11.84 FGYY76 pKa = 9.81 VKK78 pKa = 10.45 SHH80 pKa = 6.35 FLRR83 pKa = 11.84 NVVYY87 pKa = 10.6 NADD90 pKa = 3.77 ASGQMIRR97 pKa = 11.84 YY98 pKa = 7.21 NTTTCEE104 pKa = 3.79 QWFCCNFNLASYY116 pKa = 8.98 SASYY120 pKa = 10.68 NSLVPEE126 pKa = 4.85 EE127 pKa = 4.7 GGSMPINEE135 pKa = 4.23 EE136 pKa = 3.94 AEE138 pKa = 4.14 IKK140 pKa = 10.24 IGQSLLTCAQSVTKK154 pKa = 10.5 AIYY157 pKa = 10.24 AKK159 pKa = 10.64 LSTLTTRR166 pKa = 11.84 SIQGFLEE173 pKa = 4.13 CLRR176 pKa = 11.84 DD177 pKa = 4.37 AICGAFSSWLPCIRR191 pKa = 11.84 GAFAWFGNIIEE202 pKa = 4.58 VLKK205 pKa = 10.21 HH206 pKa = 4.36 WAGAAHH212 pKa = 6.65 EE213 pKa = 4.35 KK214 pKa = 10.32 LHH216 pKa = 6.25 NFLEE220 pKa = 5.29 GIEE223 pKa = 4.13 EE224 pKa = 4.32 CLYY227 pKa = 10.52 MGLGLVASTCIVALIEE243 pKa = 4.22 KK244 pKa = 9.71 FLVTMSVISGPCGRR258 pKa = 11.84 PTLFLTSAMAIISSTYY274 pKa = 9.85 LLSKK278 pKa = 10.61 AVEE281 pKa = 4.09 KK282 pKa = 11.04 SSAFTMLLGFVTQSCQTVLGSLFGKK307 pKa = 8.69 SAKK310 pKa = 10.16 GSEE313 pKa = 4.17 EE314 pKa = 3.78 AQGQFGPSAMLEE326 pKa = 4.13 SLATLVSSWSSSSVTEE342 pKa = 3.68 IGRR345 pKa = 11.84 TFGAISQIKK354 pKa = 10.0 NGIIALKK361 pKa = 10.87 DD362 pKa = 2.95 MALFVFSKK370 pKa = 9.79 LCEE373 pKa = 3.97 MASKK377 pKa = 10.97 VLGFEE382 pKa = 4.17 SQILADD388 pKa = 4.08 LSIILGEE395 pKa = 4.66 NVADD399 pKa = 4.17 WLDD402 pKa = 3.63 EE403 pKa = 4.29 CDD405 pKa = 5.4 CMLAYY410 pKa = 10.78 LLEE413 pKa = 4.75 FNSNARR419 pKa = 11.84 DD420 pKa = 3.29 IFDD423 pKa = 4.64 RR424 pKa = 11.84 LSQLIEE430 pKa = 3.79 KK431 pKa = 10.13 GKK433 pKa = 9.93 AIRR436 pKa = 11.84 MGILRR441 pKa = 11.84 TTHH444 pKa = 7.05 RR445 pKa = 11.84 GPSQVLSLVTKK456 pKa = 10.67 ALDD459 pKa = 3.84 KK460 pKa = 10.67 LTEE463 pKa = 4.0 LHH465 pKa = 6.53 NSVIMSGANSTRR477 pKa = 11.84 KK478 pKa = 7.58 TPFMLFFTGKK488 pKa = 9.86 SGVGKK493 pKa = 8.58 TSVVQRR499 pKa = 11.84 MAANWLQQEE508 pKa = 4.29 QLGSNEE514 pKa = 3.97 VYY516 pKa = 10.55 SRR518 pKa = 11.84 NGLDD522 pKa = 4.19 PFWSGYY528 pKa = 8.88 KK529 pKa = 9.34 RR530 pKa = 11.84 QAVVTYY536 pKa = 9.98 DD537 pKa = 3.62 DD538 pKa = 4.53 FGAVPGSVSNEE549 pKa = 3.34 AEE551 pKa = 4.08 IINVVSSNPHH561 pKa = 5.29 SVMMADD567 pKa = 3.86 LKK569 pKa = 10.95 EE570 pKa = 4.34 KK571 pKa = 10.52 GMYY574 pKa = 9.63 FDD576 pKa = 4.19 SRR578 pKa = 11.84 LIIASSNFLAANPEE592 pKa = 4.39 SGVHH596 pKa = 6.9 DD597 pKa = 3.7 SEE599 pKa = 5.29 AYY601 pKa = 8.16 EE602 pKa = 4.03 RR603 pKa = 11.84 RR604 pKa = 11.84 RR605 pKa = 11.84 HH606 pKa = 5.06 VVVQVSLKK614 pKa = 10.65 EE615 pKa = 3.99 DD616 pKa = 3.18 MAYY619 pKa = 10.66 DD620 pKa = 4.03 PGNPCANQRR629 pKa = 11.84 YY630 pKa = 6.5 TLLEE634 pKa = 4.11 SKK636 pKa = 11.04 APFAEE641 pKa = 4.15 KK642 pKa = 10.46 AVFEE646 pKa = 4.55 SYY648 pKa = 11.02 EE649 pKa = 4.12 EE650 pKa = 4.3 LWSHH654 pKa = 6.71 VYY656 pKa = 10.22 NAFKK660 pKa = 10.68 AHH662 pKa = 5.7 EE663 pKa = 4.34 EE664 pKa = 4.13 KK665 pKa = 10.8 EE666 pKa = 4.17 KK667 pKa = 11.15 LFLSSLPIPEE677 pKa = 4.33 RR678 pKa = 11.84 SEE680 pKa = 4.57 KK681 pKa = 10.42 EE682 pKa = 3.65 ALQALIGICVMTTSYY697 pKa = 11.14 APKK700 pKa = 10.43 AVIQYY705 pKa = 10.63 GIDD708 pKa = 3.37 HH709 pKa = 6.8 LVGYY713 pKa = 10.03 HH714 pKa = 5.42 YY715 pKa = 10.59 LISSAEE721 pKa = 3.91 HH722 pKa = 5.73 VYY724 pKa = 9.83 FWHH727 pKa = 6.78 EE728 pKa = 3.98 KK729 pKa = 9.81 GEE731 pKa = 4.11 VEE733 pKa = 4.34 IVPMHH738 pKa = 6.98 LMKK741 pKa = 10.31 LDD743 pKa = 3.89 KK744 pKa = 9.82 MDD746 pKa = 4.16 KK747 pKa = 10.07 ATMASTSLKK756 pKa = 10.05 SALMCQDD763 pKa = 3.0 MAKK766 pKa = 10.25 NFPTLNPLAVLYY778 pKa = 10.43 AKK780 pKa = 10.66 NIVIRR785 pKa = 11.84 GWVDD789 pKa = 3.22 ANLQASKK796 pKa = 10.48 KK797 pKa = 10.67 CEE799 pKa = 3.68 DD800 pKa = 3.09 SYY802 pKa = 11.2 MRR804 pKa = 11.84 EE805 pKa = 4.18 QIEE808 pKa = 5.27 SLPKK812 pKa = 8.01 WQRR815 pKa = 11.84 AYY817 pKa = 11.02 LHH819 pKa = 6.15 VLSGHH824 pKa = 5.89 IASNEE829 pKa = 3.76 TRR831 pKa = 11.84 GWFLNCLEE839 pKa = 4.29 VTKK842 pKa = 10.92 SNSRR846 pKa = 11.84 SSYY849 pKa = 7.56 IWEE852 pKa = 4.16 YY853 pKa = 11.11 KK854 pKa = 8.83 SWPMPLKK861 pKa = 10.52 LALGSFLAILAGSAIFCSLQSLWSISGNASFVAGAASIFTIGSATAQSAPPNKK914 pKa = 10.12 DD915 pKa = 2.56 GSEE918 pKa = 3.71 YY919 pKa = 10.24 TYY921 pKa = 10.86 RR922 pKa = 11.84 NKK924 pKa = 10.45 KK925 pKa = 9.16 IKK927 pKa = 9.27 IRR929 pKa = 11.84 NWEE932 pKa = 3.99 GQGPCFGDD940 pKa = 3.36 SALWIAEE947 pKa = 3.85 NCMATLVVMKK957 pKa = 10.56 DD958 pKa = 3.19 RR959 pKa = 11.84 VQVCMAPGRR968 pKa = 11.84 SFLGVNHH975 pKa = 7.1 FLRR978 pKa = 11.84 MIPNGVMVKK987 pKa = 10.46 LEE989 pKa = 3.97 TGMTEE994 pKa = 4.27 TYY996 pKa = 10.09 FVWEE1000 pKa = 4.07 KK1001 pKa = 11.3 SKK1003 pKa = 11.4 LKK1005 pKa = 10.63 LFEE1008 pKa = 4.35 NSEE1011 pKa = 3.91 IALYY1015 pKa = 10.06 TSSNLPKK1022 pKa = 10.68 APDD1025 pKa = 3.45 SLVDD1029 pKa = 3.57 RR1030 pKa = 11.84 FHH1032 pKa = 7.56 FDD1034 pKa = 4.57 LEE1036 pKa = 4.54 TLPKK1040 pKa = 9.71 TFPAQFFTYY1049 pKa = 10.37 KK1050 pKa = 9.93 FDD1052 pKa = 5.18 KK1053 pKa = 10.97 DD1054 pKa = 3.47 MQQYY1058 pKa = 9.28 VPEE1061 pKa = 5.33 LGEE1064 pKa = 4.21 LLCKK1068 pKa = 10.08 KK1069 pKa = 10.44 AEE1071 pKa = 3.99 RR1072 pKa = 11.84 ALCVVSGEE1080 pKa = 3.86 YY1081 pKa = 10.05 RR1082 pKa = 11.84 RR1083 pKa = 11.84 VISHH1087 pKa = 6.97 HH1088 pKa = 4.87 LTYY1091 pKa = 10.68 RR1092 pKa = 11.84 NPTVAGDD1099 pKa = 3.65 CGGLVLAIIEE1109 pKa = 4.67 GKK1111 pKa = 10.13 CKK1113 pKa = 10.43 LVGLHH1118 pKa = 5.58 VASDD1122 pKa = 4.23 GEE1124 pKa = 4.21 EE1125 pKa = 4.33 GAASPVPWDD1134 pKa = 3.57 PDD1136 pKa = 3.57 FKK1138 pKa = 10.82 VAQGQSDD1145 pKa = 3.92 FLLSYY1150 pKa = 10.59 DD1151 pKa = 3.08 EE1152 pKa = 4.15 WAVPKK1157 pKa = 10.85 VLGPGCKK1164 pKa = 9.5 AVGIISPEE1172 pKa = 3.89 HH1173 pKa = 5.21 TVGSGGKK1180 pKa = 8.05 TSFLEE1185 pKa = 4.94 TPIEE1189 pKa = 4.03 WQLNRR1194 pKa = 11.84 PCGKK1198 pKa = 9.87 IPSILVKK1205 pKa = 10.68 GDD1207 pKa = 2.96 VRR1209 pKa = 11.84 LAGTEE1214 pKa = 3.89 NADD1217 pKa = 3.43 YY1218 pKa = 11.31 DD1219 pKa = 4.05 PFAVGMTKK1227 pKa = 10.06 YY1228 pKa = 10.38 AKK1230 pKa = 9.77 EE1231 pKa = 4.14 AGPFEE1236 pKa = 4.95 PNGLDD1241 pKa = 3.38 RR1242 pKa = 11.84 VCEE1245 pKa = 4.4 SIAEE1249 pKa = 4.31 TWHH1252 pKa = 6.51 DD1253 pKa = 3.54 ASDD1256 pKa = 3.73 GFEE1259 pKa = 4.36 FGPVDD1264 pKa = 5.73 LEE1266 pKa = 3.99 AALNGIEE1273 pKa = 3.96 NMEE1276 pKa = 4.19 YY1277 pKa = 10.62 FDD1279 pKa = 5.56 ALVLSTSEE1287 pKa = 4.27 GYY1289 pKa = 9.69 PYY1291 pKa = 10.95 RR1292 pKa = 11.84 LDD1294 pKa = 3.64 RR1295 pKa = 11.84 KK1296 pKa = 9.85 PGEE1299 pKa = 4.15 KK1300 pKa = 10.22 GKK1302 pKa = 10.51 ARR1304 pKa = 11.84 YY1305 pKa = 9.4 VEE1307 pKa = 4.38 GEE1309 pKa = 4.09 PGNLEE1314 pKa = 3.77 ITDD1317 pKa = 3.77 EE1318 pKa = 4.96 RR1319 pKa = 11.84 ILADD1323 pKa = 2.98 IHH1325 pKa = 5.64 WFEE1328 pKa = 5.29 EE1329 pKa = 4.07 ISKK1332 pKa = 8.31 TQVPDD1337 pKa = 3.95 LYY1339 pKa = 11.0 CIEE1342 pKa = 4.27 CVKK1345 pKa = 10.9 DD1346 pKa = 3.19 EE1347 pKa = 4.34 RR1348 pKa = 11.84 LPVRR1352 pKa = 11.84 KK1353 pKa = 9.6 VIKK1356 pKa = 9.66 EE1357 pKa = 3.84 PKK1359 pKa = 9.42 SRR1361 pKa = 11.84 LFTVLPMSYY1370 pKa = 10.79 NLAIRR1375 pKa = 11.84 KK1376 pKa = 9.28 KK1377 pKa = 8.97 FLNFVRR1383 pKa = 11.84 FIMKK1387 pKa = 10.1 RR1388 pKa = 11.84 RR1389 pKa = 11.84 DD1390 pKa = 3.47 VLPCQVGINPYY1401 pKa = 9.12 SRR1403 pKa = 11.84 QWGKK1407 pKa = 9.89 VADD1410 pKa = 4.35 RR1411 pKa = 11.84 LLEE1414 pKa = 4.25 KK1415 pKa = 10.77 GNSILCCDD1423 pKa = 3.9 YY1424 pKa = 11.48 SRR1426 pKa = 11.84 FDD1428 pKa = 3.84 GFLPKK1433 pKa = 10.42 CIMVKK1438 pKa = 9.14 IAEE1441 pKa = 4.32 MFSNIVGEE1449 pKa = 4.38 TGAEE1453 pKa = 3.82 RR1454 pKa = 11.84 EE1455 pKa = 4.12 QTKK1458 pKa = 10.94 NLMLACCSRR1467 pKa = 11.84 YY1468 pKa = 10.03 AICGRR1473 pKa = 11.84 VLYY1476 pKa = 9.99 RR1477 pKa = 11.84 VEE1479 pKa = 3.99 NGIPSGFPLTVIVNSILNEE1498 pKa = 3.77 ILIKK1502 pKa = 9.66 YY1503 pKa = 8.93 AYY1505 pKa = 7.05 WKK1507 pKa = 10.63 CFEE1510 pKa = 4.28 TEE1512 pKa = 3.97 SLIRR1516 pKa = 11.84 DD1517 pKa = 3.57 HH1518 pKa = 7.27 FDD1520 pKa = 2.89 TYY1522 pKa = 11.43 VAMVVYY1528 pKa = 10.62 GDD1530 pKa = 4.73 DD1531 pKa = 3.57 NLISVSEE1538 pKa = 4.52 AISSKK1543 pKa = 10.67 FNGNFLVNFMCNLGIKK1559 pKa = 8.46 VTDD1562 pKa = 4.1 GVDD1565 pKa = 3.12 KK1566 pKa = 10.52 TKK1568 pKa = 11.06 VGIEE1572 pKa = 3.67 FRR1574 pKa = 11.84 TIEE1577 pKa = 4.41 DD1578 pKa = 3.39 CDD1580 pKa = 3.62 FLKK1583 pKa = 10.76 RR1584 pKa = 11.84 KK1585 pKa = 9.42 FKK1587 pKa = 11.07 EE1588 pKa = 4.09 NADD1591 pKa = 4.07 GTWSGVMAEE1600 pKa = 4.02 EE1601 pKa = 4.28 HH1602 pKa = 6.48 LWPQLHH1608 pKa = 5.68 FVKK1611 pKa = 10.55 AKK1613 pKa = 9.25 KK1614 pKa = 10.53 VEE1616 pKa = 3.89 MSEE1619 pKa = 5.29 AYY1621 pKa = 9.47 ISNCNNILRR1630 pKa = 11.84 EE1631 pKa = 4.01 LWLGSPEE1638 pKa = 3.5 KK1639 pKa = 10.41 AAAFRR1644 pKa = 11.84 RR1645 pKa = 11.84 EE1646 pKa = 4.41 VISKK1650 pKa = 8.78 LKK1652 pKa = 9.38 WVEE1655 pKa = 3.66 PQRR1658 pKa = 11.84 LLTISQVALFHH1669 pKa = 6.25 NEE1671 pKa = 3.79 QMNGEE1676 pKa = 4.25 HH1677 pKa = 6.9 PFVEE1681 pKa = 5.05 ACHH1684 pKa = 5.54 QLEE1687 pKa = 4.3 NLEE1690 pKa = 4.4 LMAPLEE1696 pKa = 4.37 PGMLPIKK1703 pKa = 8.67 TQEE1706 pKa = 3.72 IMPGLFVASEE1716 pKa = 4.27 KK1717 pKa = 11.02 NFTGNFDD1724 pKa = 4.07 DD1725 pKa = 5.13 YY1726 pKa = 10.45 FTISITTNRR1735 pKa = 11.84 KK1736 pKa = 9.26 FEE1738 pKa = 4.76 DD1739 pKa = 3.55 GKK1741 pKa = 10.75 GFQIIFPYY1749 pKa = 10.08 GAGRR1753 pKa = 11.84 GGLPSKK1759 pKa = 11.09 AFMEE1763 pKa = 4.21 QNVIRR1768 pKa = 11.84 KK1769 pKa = 8.88 GCAIQKK1775 pKa = 9.82 AFKK1778 pKa = 10.34 QGLEE1782 pKa = 4.44 KK1783 pKa = 10.48 GNKK1786 pKa = 8.1 MLFISQSSVIPAYY1799 pKa = 10.56 VFAIMLYY1806 pKa = 9.93 RR1807 pKa = 11.84 SVDD1810 pKa = 3.43 RR1811 pKa = 11.84 LPRR1814 pKa = 11.84 ALSNKK1819 pKa = 9.81 ALTSALGICKK1829 pKa = 10.1 KK1830 pKa = 10.59 LSYY1833 pKa = 9.98 LPKK1836 pKa = 10.5 DD1837 pKa = 3.88 FPDD1840 pKa = 3.81 LFF1842 pKa = 6.15
Molecular weight: 205.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.913
IPC2_protein 6.008
IPC_protein 6.046
Toseland 6.237
ProMoST 6.415
Dawson 6.338
Bjellqvist 6.364
Wikipedia 6.338
Rodwell 6.326
Grimsley 6.326
Solomon 6.338
Lehninger 6.326
Nozaki 6.62
DTASelect 6.781
Thurlkill 6.795
EMBOSS 6.751
Sillero 6.722
Patrickios 4.075
IPC_peptide 6.351
IPC2_peptide 6.605
IPC2.peptide.svr19 6.632
Protein with the highest isoelectric point:
>sp|Q76L40|POL1_BBWVS RNA1 polyprotein OS=Broad bean wilt virus 1 (strain Spinach/United States/ATCC PV-132/1963) OX=649895 GN=RNA1 PE=3 SV=1
MM1 pKa = 7.73 PLILLWFCFLCMIYY15 pKa = 10.49 VILKK19 pKa = 10.28 LEE21 pKa = 3.83 YY22 pKa = 9.08 TYY24 pKa = 11.26 VVKK27 pKa = 10.64 PFLKK31 pKa = 9.87 TAFLNTTSHH40 pKa = 6.67 YY41 pKa = 9.58 SDD43 pKa = 3.74 EE44 pKa = 4.23 YY45 pKa = 9.95 LRR47 pKa = 11.84 EE48 pKa = 3.85 YY49 pKa = 11.0 SGIRR53 pKa = 11.84 WLHH56 pKa = 4.58 TFKK59 pKa = 10.66 EE60 pKa = 4.47 YY61 pKa = 10.55 RR62 pKa = 11.84 EE63 pKa = 3.91 HH64 pKa = 6.95 TIFFNFPSFYY74 pKa = 9.92 CSPGSGIKK82 pKa = 8.36 HH83 pKa = 5.53 TLSEE87 pKa = 4.65 LIKK90 pKa = 10.26 MEE92 pKa = 4.18 AEE94 pKa = 3.71 RR95 pKa = 11.84 VNLTKK100 pKa = 10.61 TEE102 pKa = 4.38 TIPKK106 pKa = 10.1 DD107 pKa = 3.19 ILLEE111 pKa = 3.84 RR112 pKa = 11.84 AKK114 pKa = 10.5 NYY116 pKa = 10.13 RR117 pKa = 11.84 VAQEE121 pKa = 4.34 SNKK124 pKa = 10.38 SLLPQVEE131 pKa = 4.29 DD132 pKa = 3.99 LYY134 pKa = 11.37 EE135 pKa = 3.83 VSKK138 pKa = 9.88 WKK140 pKa = 10.33 RR141 pKa = 11.84 AISSLQKK148 pKa = 10.77 GEE150 pKa = 3.95 PSFVRR155 pKa = 11.84 TSEE158 pKa = 4.12 VAIGTMSGAGKK169 pKa = 8.82 MRR171 pKa = 11.84 IKK173 pKa = 10.89 VPVVKK178 pKa = 10.33 SYY180 pKa = 11.06 EE181 pKa = 4.07 EE182 pKa = 4.0 EE183 pKa = 4.13 VADD186 pKa = 3.67 MRR188 pKa = 11.84 LSQKK192 pKa = 9.59 VRR194 pKa = 11.84 AKK196 pKa = 10.57 ADD198 pKa = 3.65 QIVVAAIEE206 pKa = 4.07 IVNDD210 pKa = 3.72 GFASVNSDD218 pKa = 3.09 VTLAASLYY226 pKa = 9.92 DD227 pKa = 3.76 KK228 pKa = 10.51 RR229 pKa = 11.84 HH230 pKa = 4.76 KK231 pKa = 9.62 TIASSFKK238 pKa = 9.98 GAYY241 pKa = 9.62 ASRR244 pKa = 11.84 ASGTPSHH251 pKa = 6.35 VVFYY255 pKa = 6.93 PTHH258 pKa = 6.28 RR259 pKa = 11.84 VAPGDD264 pKa = 3.77 NPNDD268 pKa = 3.9 TLEE271 pKa = 4.26 LSAVSRR277 pKa = 11.84 DD278 pKa = 3.04 SDD280 pKa = 3.41 FDD282 pKa = 4.55 EE283 pKa = 5.13 NFTLANFSVRR293 pKa = 11.84 TVYY296 pKa = 10.96 AKK298 pKa = 10.81 AKK300 pKa = 7.99 GPEE303 pKa = 4.31 VIRR306 pKa = 11.84 EE307 pKa = 4.16 TQHH310 pKa = 7.14 LLNCKK315 pKa = 10.44 LEE317 pKa = 4.54 DD318 pKa = 3.76 LVKK321 pKa = 10.24 AQQFASDD328 pKa = 3.94 EE329 pKa = 4.35 QVVLALPRR337 pKa = 11.84 VYY339 pKa = 10.66 PKK341 pKa = 11.1 VNLDD345 pKa = 3.6 NYY347 pKa = 10.21 VMPGPDD353 pKa = 2.97 NVTKK357 pKa = 10.82 QEE359 pKa = 4.05 GEE361 pKa = 4.06 YY362 pKa = 9.68 SAKK365 pKa = 10.33 GIHH368 pKa = 5.62 FRR370 pKa = 11.84 KK371 pKa = 9.96 PIFNGSEE378 pKa = 3.59 IVLNATSKK386 pKa = 10.89 LPSSKK391 pKa = 10.49 SRR393 pKa = 11.84 GISKK397 pKa = 9.99 SEE399 pKa = 4.14 KK400 pKa = 9.75 IDD402 pKa = 4.53 DD403 pKa = 4.56 LGCMSDD409 pKa = 4.65 EE410 pKa = 4.04 EE411 pKa = 5.69 GIDD414 pKa = 3.59 YY415 pKa = 10.59 KK416 pKa = 11.14 YY417 pKa = 11.07 GQALMEE423 pKa = 4.55 EE424 pKa = 4.95 DD425 pKa = 3.65 VLEE428 pKa = 4.61 AQVDD432 pKa = 3.99 MFPLHH437 pKa = 6.3 NVAEE441 pKa = 4.49 TMRR444 pKa = 11.84 LLFSGVSTIPMNVVEE459 pKa = 4.39 GTKK462 pKa = 10.14 ISVAYY467 pKa = 10.41 LNEE470 pKa = 4.38 LATHH474 pKa = 7.0 PGVHH478 pKa = 5.72 VPILNMLGRR487 pKa = 11.84 IPGSILARR495 pKa = 11.84 VHH497 pKa = 6.69 CEE499 pKa = 3.58 VAPTCGIGLAATYY512 pKa = 11.1 VEE514 pKa = 4.64 GNEE517 pKa = 4.15 SAALGTDD524 pKa = 3.79 LGRR527 pKa = 11.84 LLGIQHH533 pKa = 6.01 VKK535 pKa = 9.11 WNPAIEE541 pKa = 4.08 PVKK544 pKa = 10.28 EE545 pKa = 4.24 FRR547 pKa = 11.84 FKK549 pKa = 10.86 PFSCVDD555 pKa = 3.31 WWNMHH560 pKa = 5.71 YY561 pKa = 10.48 LGSSKK566 pKa = 10.19 FSPVLAFVCLSKK578 pKa = 10.36 WINPPKK584 pKa = 10.47 GEE586 pKa = 4.31 CKK588 pKa = 9.56 MSYY591 pKa = 10.98 ALYY594 pKa = 10.16 FEE596 pKa = 5.35 PDD598 pKa = 3.26 IILPRR603 pKa = 11.84 QIASLNSVPSFMLRR617 pKa = 11.84 KK618 pKa = 9.51 EE619 pKa = 4.01 LGTLSFKK626 pKa = 10.55 QGEE629 pKa = 4.03 RR630 pKa = 11.84 RR631 pKa = 11.84 AYY633 pKa = 9.77 AFEE636 pKa = 4.43 VNFGKK641 pKa = 9.5 PQVEE645 pKa = 4.59 GKK647 pKa = 10.27 SVTLNFASAYY657 pKa = 9.64 CGLSQYY663 pKa = 9.59 MEE665 pKa = 4.2 SDD667 pKa = 3.02 IVIDD671 pKa = 4.03 LTLMSSPMMGGTFTLAYY688 pKa = 10.13 VAGSYY693 pKa = 10.41 LKK695 pKa = 10.44 SIKK698 pKa = 10.45 NMQFLDD704 pKa = 4.25 ALPHH708 pKa = 5.13 ITFNFEE714 pKa = 3.89 KK715 pKa = 10.16 GGKK718 pKa = 6.39 STRR721 pKa = 11.84 SLRR724 pKa = 11.84 FPSRR728 pKa = 11.84 LFPTYY733 pKa = 10.47 QSLDD737 pKa = 3.19 RR738 pKa = 11.84 WDD740 pKa = 4.73 LNASRR745 pKa = 11.84 EE746 pKa = 4.16 DD747 pKa = 3.87 DD748 pKa = 3.79 VSGHH752 pKa = 5.31 FVLYY756 pKa = 10.64 QRR758 pKa = 11.84 DD759 pKa = 4.02 TVSSALEE766 pKa = 3.97 GDD768 pKa = 3.61 LVFRR772 pKa = 11.84 VSARR776 pKa = 11.84 VSGEE780 pKa = 3.5 PMMHH784 pKa = 6.09 GVSVGYY790 pKa = 7.44 PTTLTRR796 pKa = 11.84 ATTGKK801 pKa = 9.41 MSSRR805 pKa = 11.84 SLGEE809 pKa = 3.98 KK810 pKa = 8.59 VRR812 pKa = 11.84 KK813 pKa = 9.0 PIGLAKK819 pKa = 10.26 GQAHH823 pKa = 6.56 MNLADD828 pKa = 4.14 YY829 pKa = 10.92 KK830 pKa = 10.63 RR831 pKa = 11.84 VFYY834 pKa = 10.61 PMAEE838 pKa = 4.02 WIYY841 pKa = 11.02 SSEE844 pKa = 4.07 KK845 pKa = 9.72 YY846 pKa = 9.49 EE847 pKa = 4.14 GRR849 pKa = 11.84 RR850 pKa = 11.84 EE851 pKa = 3.84 DD852 pKa = 4.49 RR853 pKa = 11.84 DD854 pKa = 3.52 ILKK857 pKa = 10.57 LLLKK861 pKa = 9.9 MRR863 pKa = 11.84 LDD865 pKa = 3.66 GTKK868 pKa = 9.06 ATEE871 pKa = 4.26 DD872 pKa = 3.41 FRR874 pKa = 11.84 IVHH877 pKa = 5.86 SPLVRR882 pKa = 11.84 VLQNCAWLRR891 pKa = 11.84 GTIHH895 pKa = 6.34 FKK897 pKa = 10.41 IVVRR901 pKa = 11.84 ANSEE905 pKa = 3.74 MMSYY909 pKa = 10.68 QRR911 pKa = 11.84 TSQVHH916 pKa = 4.04 VTAHH920 pKa = 5.94 EE921 pKa = 4.31 NSLSSNEE928 pKa = 4.22 FFSGMLTATSGEE940 pKa = 4.19 LEE942 pKa = 4.04 FSKK945 pKa = 10.81 EE946 pKa = 3.74 VVGPVEE952 pKa = 5.02 GFSSMGWNVQGNKK965 pKa = 9.53 KK966 pKa = 9.99 FYY968 pKa = 10.02 KK969 pKa = 10.44 LCIALGNVHH978 pKa = 6.74 EE979 pKa = 4.35 YY980 pKa = 10.77 EE981 pKa = 4.2 AVKK984 pKa = 10.92 VMASFGDD991 pKa = 3.81 DD992 pKa = 3.42 VEE994 pKa = 4.96 FAGQQKK1000 pKa = 10.06 AGHH1003 pKa = 5.95 YY1004 pKa = 9.07 ALEE1007 pKa = 4.57 RR1008 pKa = 11.84 GVSVFKK1014 pKa = 10.46 EE1015 pKa = 4.16 FKK1017 pKa = 10.01 YY1018 pKa = 10.99
Molecular weight: 114.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.088
IPC2_protein 7.527
IPC_protein 7.366
Toseland 7.205
ProMoST 8.009
Dawson 8.112
Bjellqvist 8.39
Wikipedia 8.039
Rodwell 8.141
Grimsley 7.293
Solomon 8.156
Lehninger 8.156
Nozaki 8.507
DTASelect 8.185
Thurlkill 8.244
EMBOSS 8.317
Sillero 8.521
Patrickios 4.317
IPC_peptide 8.141
IPC2_peptide 7.307
IPC2.peptide.svr19 7.516
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
1
3
3788
928
1842
1262.7
141.14
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.445 ± 0.219
1.848 ± 0.453
4.118 ± 0.126
7.154 ± 0.109
5.069 ± 0.176
6.705 ± 0.078
2.138 ± 0.149
5.148 ± 0.535
6.679 ± 0.001
9.24 ± 0.297
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.115 ± 0.054
4.092 ± 0.123
4.25 ± 0.112
2.798 ± 0.057
4.541 ± 0.371
8.712 ± 0.261
4.461 ± 0.21
7.524 ± 0.661
1.373 ± 0.235
3.59 ± 0.218
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here