Mycobacterium virus Dlane 
Average proteome isoelectric point is 6.13 
Get precalculated fractions of proteins 
 
  
    Acidic  
 
  
    pI < 6.8  
 
  
    6.8-7.4  
 
  
    pI > 7.4  
 
  
    Basic  
    
 
  
    All  
 
Note: above files contain also dissociation constants (pKa) 
Virtual 2D-PAGE plot for 105 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 Summary statistics related to proteome-wise predictions 
Protein with the lowest isoelectric point: 
>tr|G1D1F9|G1D1F9_9CAUD Methylase OS=Mycobacterium virus Dlane OX=1034101 GN=65 PE=3 SV=1MM1 pKa = 7.97  PDD3 pKa = 2.9  VHH5 pKa = 8.39  IGFTGTRR12 pKa = 11.84  DD13 pKa = 3.09  AMPQVRR19 pKa = 11.84  RR20 pKa = 11.84  PDD22 pKa = 3.7  GDD24 pKa = 3.94  DD25 pKa = 3.25  PAMNASEE32 pKa = 5.57  DD33 pKa = 3.63  GLEE36 pKa = 4.36  PLGEE40 pKa = 4.45  APDD43 pKa = 4.44  LGAPHH48 pKa = 6.8  YY49 pKa = 10.26  PMSAEE54 pKa = 4.24  VTFHH58 pKa = 7.62  RR59 pKa = 11.84  ARR61 pKa = 11.84  CTKK64 pKa = 10.5  CGDD67 pKa = 3.08  IEE69 pKa = 4.31  TDD71 pKa = 3.37  YY72 pKa = 11.76  GDD74 pKa = 4.22  FSAYY78 pKa = 10.11  SDD80 pKa = 3.39  PGGAINAVLAAADD93 pKa = 3.49  WFGRR97 pKa = 11.84  SAPTGEE103 pKa = 5.08  LIDD106 pKa = 4.08  FGGGRR111 pKa = 11.84  MGQRR115 pKa = 11.84  YY116 pKa = 7.81  QLVEE120 pKa = 4.46  LLCPDD125 pKa = 3.92  CQRR128 pKa = 11.84  CEE130 pKa = 3.95  VCGTAKK136 pKa = 10.4  AYY138 pKa = 8.58  PINDD142 pKa = 3.59  HH143 pKa = 6.44  LVCEE147 pKa = 4.23  DD148 pKa = 4.1  HH149 pKa = 7.29  EE150 pKa = 4.31  DD151 pKa = 3.94  HH152 pKa = 6.79  EE153 pKa = 5.25  FEE155 pKa = 4.96  AAPP158 pKa = 3.95  
 17.06 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  3.998 
IPC2_protein 4.279 
IPC_protein 4.24 
Toseland    4.062 
ProMoST     4.393 
Dawson      4.228 
Bjellqvist  4.368 
Wikipedia   4.139 
Rodwell     4.088 
Grimsley    3.973 
Solomon     4.215 
Lehninger   4.177 
Nozaki      4.329 
DTASelect   4.546 
Thurlkill   4.088 
EMBOSS      4.151 
Sillero     4.368 
Patrickios  1.99 
IPC_peptide 4.215 
IPC2_peptide  4.355 
IPC2.peptide.svr19  4.244 
 Protein with the highest isoelectric point: 
>tr|G1D1F0|G1D1F0_9CAUD Uncharacterized protein OS=Mycobacterium virus Dlane OX=1034101 GN=56 PE=4 SV=1MM1 pKa = 7.67  SNGNRR6 pKa = 11.84  LTDD9 pKa = 3.64  EE10 pKa = 4.05  QVKK13 pKa = 9.95  MILSMTRR20 pKa = 11.84  DD21 pKa = 3.58  GFSAKK26 pKa = 10.29  HH27 pKa = 5.47  IGEE30 pKa = 4.4  VVGCSPRR37 pKa = 11.84  TVTRR41 pKa = 11.84  VRR43 pKa = 11.84  AAADD47 pKa = 3.34  ARR49 pKa = 11.84  VMNPRR54 pKa = 11.84  RR55 pKa = 11.84  FTPLTADD62 pKa = 3.02  QLEE65 pKa = 4.26  FAEE68 pKa = 4.73  YY69 pKa = 10.48  LLEE72 pKa = 5.95  DD73 pKa = 3.83  GASYY77 pKa = 11.1  QEE79 pKa = 4.12  VARR82 pKa = 11.84  TLGVSRR88 pKa = 11.84  TTIEE92 pKa = 3.54  RR93 pKa = 11.84  HH94 pKa = 4.64  FPGRR98 pKa = 11.84  AWTKK102 pKa = 9.06  RR103 pKa = 11.84  QAAEE107 pKa = 4.07  FTALRR112 pKa = 11.84  KK113 pKa = 9.66  KK114 pKa = 10.28  FRR116 pKa = 11.84  RR117 pKa = 11.84  LEE119 pKa = 3.98  ASS121 pKa = 3.05  
 13.7 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.318 
IPC2_protein 9.399 
IPC_protein 10.058 
Toseland    10.526 
ProMoST     10.189 
Dawson      10.613 
Bjellqvist  10.321 
Wikipedia   10.818 
Rodwell     10.774 
Grimsley    10.657 
Solomon     10.745 
Lehninger   10.716 
Nozaki      10.511 
DTASelect   10.306 
Thurlkill   10.526 
EMBOSS      10.921 
Sillero     10.555 
Patrickios  10.54 
IPC_peptide 10.745 
IPC2_peptide  9.194 
IPC2.peptide.svr19  8.683 
 Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
   
  
General Statistics 
    
      
        Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
 
     
    
      
        105 
0
105 
18654
30
1160
177.7
19.43
          
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
 
     
    
                 
        10.561 ± 0.447
1.185 ± 0.163
6.889 ± 0.229
5.774 ± 0.302
2.943 ± 0.205
8.888 ± 0.539
2.203 ± 0.173
4.283 ± 0.21
3.147 ± 0.192
7.403 ± 0.214
          
     
  
  
  
      
          
        Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
 
     
    
                 
        2.252 ± 0.139
3.404 ± 0.176
6.176 ± 0.196
3.318 ± 0.183
6.996 ± 0.4
5.779 ± 0.248
6.669 ± 0.274
7.221 ± 0.252
2.278 ± 0.139
2.632 ± 0.185
          
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein levelMost of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here