Mycobacterium virus Dlane
Average proteome isoelectric point is 6.13
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 105 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G1D1F9|G1D1F9_9CAUD Methylase OS=Mycobacterium virus Dlane OX=1034101 GN=65 PE=3 SV=1
MM1 pKa = 7.97 PDD3 pKa = 2.9 VHH5 pKa = 8.39 IGFTGTRR12 pKa = 11.84 DD13 pKa = 3.09 AMPQVRR19 pKa = 11.84 RR20 pKa = 11.84 PDD22 pKa = 3.7 GDD24 pKa = 3.94 DD25 pKa = 3.25 PAMNASEE32 pKa = 5.57 DD33 pKa = 3.63 GLEE36 pKa = 4.36 PLGEE40 pKa = 4.45 APDD43 pKa = 4.44 LGAPHH48 pKa = 6.8 YY49 pKa = 10.26 PMSAEE54 pKa = 4.24 VTFHH58 pKa = 7.62 RR59 pKa = 11.84 ARR61 pKa = 11.84 CTKK64 pKa = 10.5 CGDD67 pKa = 3.08 IEE69 pKa = 4.31 TDD71 pKa = 3.37 YY72 pKa = 11.76 GDD74 pKa = 4.22 FSAYY78 pKa = 10.11 SDD80 pKa = 3.39 PGGAINAVLAAADD93 pKa = 3.49 WFGRR97 pKa = 11.84 SAPTGEE103 pKa = 5.08 LIDD106 pKa = 4.08 FGGGRR111 pKa = 11.84 MGQRR115 pKa = 11.84 YY116 pKa = 7.81 QLVEE120 pKa = 4.46 LLCPDD125 pKa = 3.92 CQRR128 pKa = 11.84 CEE130 pKa = 3.95 VCGTAKK136 pKa = 10.4 AYY138 pKa = 8.58 PINDD142 pKa = 3.59 HH143 pKa = 6.44 LVCEE147 pKa = 4.23 DD148 pKa = 4.1 HH149 pKa = 7.29 EE150 pKa = 4.31 DD151 pKa = 3.94 HH152 pKa = 6.79 EE153 pKa = 5.25 FEE155 pKa = 4.96 AAPP158 pKa = 3.95
Molecular weight: 17.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.998
IPC2_protein 4.279
IPC_protein 4.24
Toseland 4.062
ProMoST 4.393
Dawson 4.228
Bjellqvist 4.368
Wikipedia 4.139
Rodwell 4.088
Grimsley 3.973
Solomon 4.215
Lehninger 4.177
Nozaki 4.329
DTASelect 4.546
Thurlkill 4.088
EMBOSS 4.151
Sillero 4.368
Patrickios 1.99
IPC_peptide 4.215
IPC2_peptide 4.355
IPC2.peptide.svr19 4.244
Protein with the highest isoelectric point:
>tr|G1D1F0|G1D1F0_9CAUD Uncharacterized protein OS=Mycobacterium virus Dlane OX=1034101 GN=56 PE=4 SV=1
MM1 pKa = 7.67 SNGNRR6 pKa = 11.84 LTDD9 pKa = 3.64 EE10 pKa = 4.05 QVKK13 pKa = 9.95 MILSMTRR20 pKa = 11.84 DD21 pKa = 3.58 GFSAKK26 pKa = 10.29 HH27 pKa = 5.47 IGEE30 pKa = 4.4 VVGCSPRR37 pKa = 11.84 TVTRR41 pKa = 11.84 VRR43 pKa = 11.84 AAADD47 pKa = 3.34 ARR49 pKa = 11.84 VMNPRR54 pKa = 11.84 RR55 pKa = 11.84 FTPLTADD62 pKa = 3.02 QLEE65 pKa = 4.26 FAEE68 pKa = 4.73 YY69 pKa = 10.48 LLEE72 pKa = 5.95 DD73 pKa = 3.83 GASYY77 pKa = 11.1 QEE79 pKa = 4.12 VARR82 pKa = 11.84 TLGVSRR88 pKa = 11.84 TTIEE92 pKa = 3.54 RR93 pKa = 11.84 HH94 pKa = 4.64 FPGRR98 pKa = 11.84 AWTKK102 pKa = 9.06 RR103 pKa = 11.84 QAAEE107 pKa = 4.07 FTALRR112 pKa = 11.84 KK113 pKa = 9.66 KK114 pKa = 10.28 FRR116 pKa = 11.84 RR117 pKa = 11.84 LEE119 pKa = 3.98 ASS121 pKa = 3.05
Molecular weight: 13.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.318
IPC2_protein 9.399
IPC_protein 10.058
Toseland 10.526
ProMoST 10.189
Dawson 10.613
Bjellqvist 10.321
Wikipedia 10.818
Rodwell 10.774
Grimsley 10.657
Solomon 10.745
Lehninger 10.716
Nozaki 10.511
DTASelect 10.306
Thurlkill 10.526
EMBOSS 10.921
Sillero 10.555
Patrickios 10.54
IPC_peptide 10.745
IPC2_peptide 9.194
IPC2.peptide.svr19 8.683
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
105
0
105
18654
30
1160
177.7
19.43
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.561 ± 0.447
1.185 ± 0.163
6.889 ± 0.229
5.774 ± 0.302
2.943 ± 0.205
8.888 ± 0.539
2.203 ± 0.173
4.283 ± 0.21
3.147 ± 0.192
7.403 ± 0.214
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.252 ± 0.139
3.404 ± 0.176
6.176 ± 0.196
3.318 ± 0.183
6.996 ± 0.4
5.779 ± 0.248
6.669 ± 0.274
7.221 ± 0.252
2.278 ± 0.139
2.632 ± 0.185
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here