Blautia obeum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Blautia

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3072 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A173ZKG0|A0A173ZKG0_9FIRM D-ribose-binding periplasmic protein OS=Blautia obeum OX=40520 GN=rbsB_3 PE=4 SV=1
MM1 pKa = 7.24NFKK4 pKa = 10.47KK5 pKa = 10.65VVALSICAAMVAGMSASTVMAADD28 pKa = 4.94LPDD31 pKa = 3.31QFKK34 pKa = 10.95DD35 pKa = 3.62LKK37 pKa = 11.23ANEE40 pKa = 4.36AYY42 pKa = 8.75EE43 pKa = 4.29FPMMVKK49 pKa = 10.25SFQSTYY55 pKa = 9.19WDD57 pKa = 3.55AAQEE61 pKa = 4.29GMKK64 pKa = 10.24KK65 pKa = 10.02AAEE68 pKa = 4.15EE69 pKa = 4.14LGVTYY74 pKa = 8.78TAQGPNSEE82 pKa = 4.82SDD84 pKa = 2.96IADD87 pKa = 3.4QVNMINTAIASNPKK101 pKa = 10.27GLGLAACDD109 pKa = 3.57TSSVLDD115 pKa = 4.0ALNDD119 pKa = 4.03CVSKK123 pKa = 10.9GIPVVTFDD131 pKa = 4.4TGIADD136 pKa = 3.76APEE139 pKa = 4.01GSIACEE145 pKa = 3.94VCTDD149 pKa = 3.36NAQAGSVAAEE159 pKa = 3.86NMYY162 pKa = 10.78NAIKK166 pKa = 10.63DD167 pKa = 3.65VVANAEE173 pKa = 4.12GQVVIGEE180 pKa = 4.33VNQDD184 pKa = 2.58ATAQNIQQRR193 pKa = 11.84GGGFIDD199 pKa = 5.05KK200 pKa = 9.99MIEE203 pKa = 3.71LLQADD208 pKa = 4.71GKK210 pKa = 8.48TVAVAGNEE218 pKa = 4.32FYY220 pKa = 11.79VNAAKK225 pKa = 10.67NADD228 pKa = 3.64ATEE231 pKa = 4.09ADD233 pKa = 3.84ADD235 pKa = 4.04VVIQVAVPAQTTVEE249 pKa = 4.15LCSNEE254 pKa = 3.73AQAILSKK261 pKa = 9.86EE262 pKa = 3.65NCIAIFGSNQTAAEE276 pKa = 4.34GVLAANANLNVLGSDD291 pKa = 3.4AAAGDD296 pKa = 4.03VIGVGFDD303 pKa = 2.86AGSIIKK309 pKa = 10.33AAVQDD314 pKa = 4.17GTFLGAVTQSPLMMGYY330 pKa = 9.54YY331 pKa = 10.51AIYY334 pKa = 10.78ALTAAANGQEE344 pKa = 4.48LEE346 pKa = 4.39DD347 pKa = 4.29VPTDD351 pKa = 4.3GYY353 pKa = 10.72WYY355 pKa = 9.99DD356 pKa = 3.67STNMDD361 pKa = 4.74DD362 pKa = 5.75EE363 pKa = 5.13EE364 pKa = 4.43IAPNLYY370 pKa = 10.36DD371 pKa = 3.33

Molecular weight: 38.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A174R7D6|A0A174R7D6_9FIRM Uncharacterized protein OS=Blautia obeum OX=40520 GN=DW021_12595 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.86KK9 pKa = 8.2RR10 pKa = 11.84SRR12 pKa = 11.84AKK14 pKa = 9.27VHH16 pKa = 5.81GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.15GGRR28 pKa = 11.84KK29 pKa = 8.69VLAARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.68GRR39 pKa = 11.84KK40 pKa = 8.28QLSAA44 pKa = 3.9

Molecular weight: 5.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3072

0

3072

989712

29

2594

322.2

36.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.348 ± 0.04

1.538 ± 0.021

5.753 ± 0.036

7.726 ± 0.055

4.02 ± 0.035

7.024 ± 0.04

1.797 ± 0.019

7.289 ± 0.041

7.066 ± 0.046

8.831 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.182 ± 0.021

4.345 ± 0.03

3.256 ± 0.021

3.382 ± 0.03

4.297 ± 0.039

5.726 ± 0.034

5.54 ± 0.041

6.835 ± 0.036

0.927 ± 0.018

4.118 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski