Streptomyces sp. Ru87

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6048 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2B8B3L1|A0A2B8B3L1_9ACTN Phage tail protein OS=Streptomyces sp. Ru87 OX=2044307 GN=CRI70_01715 PE=4 SV=1
MM1 pKa = 7.44LTTDD5 pKa = 4.27YY6 pKa = 11.51VPGAPVWIDD15 pKa = 3.45LGTPDD20 pKa = 4.01IEE22 pKa = 4.41AAVGFYY28 pKa = 10.67GHH30 pKa = 5.9VFGWVFEE37 pKa = 4.51SAGPGAGGYY46 pKa = 11.37GMFKK50 pKa = 10.73LGGEE54 pKa = 4.39TVAAIGPMSEE64 pKa = 4.32EE65 pKa = 4.51DD66 pKa = 4.38SDD68 pKa = 4.65PGWSLYY74 pKa = 10.55FHH76 pKa = 6.86TSDD79 pKa = 4.59ADD81 pKa = 3.26ATTRR85 pKa = 11.84AVEE88 pKa = 4.03QAGGTVEE95 pKa = 4.45LPPMGVFDD103 pKa = 5.3LGRR106 pKa = 11.84MAHH109 pKa = 5.29YY110 pKa = 8.33TDD112 pKa = 3.87PAGARR117 pKa = 11.84FAAWEE122 pKa = 3.81PAGNGGLDD130 pKa = 3.27AVGEE134 pKa = 4.27PGTLCWTEE142 pKa = 4.69LYY144 pKa = 9.59STDD147 pKa = 3.41AATARR152 pKa = 11.84DD153 pKa = 4.71FYY155 pKa = 10.92RR156 pKa = 11.84AVFGWVLQDD165 pKa = 3.48APVGGGLDD173 pKa = 3.61YY174 pKa = 11.57ALVSPAGDD182 pKa = 4.09GPDD185 pKa = 3.46TSQGGIMQLPAEE197 pKa = 4.75NIAAGTTSDD206 pKa = 2.87WHH208 pKa = 6.91PYY210 pKa = 10.06FEE212 pKa = 5.54VADD215 pKa = 4.14CDD217 pKa = 3.65ATLAAAAGRR226 pKa = 11.84GGTVLIPAEE235 pKa = 4.1HH236 pKa = 6.51APGVGRR242 pKa = 11.84LAMLKK247 pKa = 10.43DD248 pKa = 3.49PFGAPFALLTPDD260 pKa = 3.34TAAA263 pKa = 4.36

Molecular weight: 27.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2B8AUU9|A0A2B8AUU9_9ACTN Complex1_30kDa domain-containing protein OS=Streptomyces sp. Ru87 OX=2044307 GN=CRI70_21130 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILATRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.48GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6048

0

6048

1998184

31

3815

330.4

35.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.768 ± 0.05

0.797 ± 0.009

6.03 ± 0.024

5.949 ± 0.031

2.669 ± 0.017

10.196 ± 0.034

2.252 ± 0.015

2.857 ± 0.019

1.952 ± 0.023

10.086 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.67 ± 0.014

1.692 ± 0.015

6.39 ± 0.032

2.555 ± 0.02

8.504 ± 0.038

4.974 ± 0.022

5.901 ± 0.026

8.267 ± 0.027

1.478 ± 0.012

2.013 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski