Amino acid dipepetide frequency for Streptomyces sp. Ru87

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
23.202AlaAla: 23.202 ± 0.173
1.059AlaCys: 1.059 ± 0.022
8.501AlaAsp: 8.501 ± 0.073
9.486AlaGlu: 9.486 ± 0.097
3.365AlaPhe: 3.365 ± 0.039
15.315AlaGly: 15.315 ± 0.122
2.695AlaHis: 2.695 ± 0.041
2.838AlaIle: 2.838 ± 0.046
2.51AlaLys: 2.51 ± 0.045
13.872AlaLeu: 13.872 ± 0.107
2.325AlaMet: 2.325 ± 0.033
1.78AlaAsn: 1.78 ± 0.029
7.606AlaPro: 7.606 ± 0.083
3.234AlaGln: 3.234 ± 0.039
10.878AlaArg: 10.878 ± 0.091
5.663AlaSer: 5.663 ± 0.058
6.308AlaThr: 6.308 ± 0.062
12.711AlaVal: 12.711 ± 0.098
1.733AlaTrp: 1.733 ± 0.029
2.601AlaTyr: 2.601 ± 0.038
0.0AlaXaa: 0.0 ± 0.0
Cys
1.149CysAla: 1.149 ± 0.024
0.106CysCys: 0.106 ± 0.007
0.498CysAsp: 0.498 ± 0.015
0.425CysGlu: 0.425 ± 0.015
0.223CysPhe: 0.223 ± 0.009
0.98CysGly: 0.98 ± 0.023
0.186CysHis: 0.186 ± 0.01
0.146CysIle: 0.146 ± 0.008
0.105CysLys: 0.105 ± 0.007
0.737CysLeu: 0.737 ± 0.021
0.124CysMet: 0.124 ± 0.007
0.134CysAsn: 0.134 ± 0.007
0.491CysPro: 0.491 ± 0.018
0.145CysGln: 0.145 ± 0.008
0.644CysArg: 0.644 ± 0.018
0.433CysSer: 0.433 ± 0.014
0.51CysThr: 0.51 ± 0.016
0.638CysVal: 0.638 ± 0.019
0.134CysTrp: 0.134 ± 0.008
0.158CysTyr: 0.158 ± 0.009
0.0CysXaa: 0.0 ± 0.0
Asp
7.63AspAla: 7.63 ± 0.065
0.443AspCys: 0.443 ± 0.016
3.526AspAsp: 3.526 ± 0.05
3.851AspGlu: 3.851 ± 0.053
1.632AspPhe: 1.632 ± 0.03
7.076AspGly: 7.076 ± 0.068
1.353AspHis: 1.353 ± 0.028
1.848AspIle: 1.848 ± 0.031
1.088AspLys: 1.088 ± 0.029
5.824AspLeu: 5.824 ± 0.056
0.797AspMet: 0.797 ± 0.021
0.962AspAsn: 0.962 ± 0.024
4.721AspPro: 4.721 ± 0.051
1.344AspGln: 1.344 ± 0.027
5.421AspArg: 5.421 ± 0.063
2.718AspSer: 2.718 ± 0.036
3.377AspThr: 3.377 ± 0.045
4.562AspVal: 4.562 ± 0.043
1.027AspTrp: 1.027 ± 0.025
1.095AspTyr: 1.095 ± 0.025
0.0AspXaa: 0.0 ± 0.0
Glu
7.665GluAla: 7.665 ± 0.078
0.364GluCys: 0.364 ± 0.013
3.2GluAsp: 3.2 ± 0.043
3.89GluGlu: 3.89 ± 0.063
1.428GluPhe: 1.428 ± 0.028
4.395GluGly: 4.395 ± 0.055
1.63GluHis: 1.63 ± 0.032
2.208GluIle: 2.208 ± 0.033
1.521GluLys: 1.521 ± 0.036
6.84GluLeu: 6.84 ± 0.079
0.886GluMet: 0.886 ± 0.023
1.111GluAsn: 1.111 ± 0.022
3.916GluPro: 3.916 ± 0.056
2.39GluGln: 2.39 ± 0.038
5.951GluArg: 5.951 ± 0.061
2.751GluSer: 2.751 ± 0.038
3.103GluThr: 3.103 ± 0.047
4.391GluVal: 4.391 ± 0.048
0.773GluTrp: 0.773 ± 0.019
1.074GluTyr: 1.074 ± 0.024
0.0GluXaa: 0.0 ± 0.0
Phe
3.554PheAla: 3.554 ± 0.05
0.267PheCys: 0.267 ± 0.01
1.897PheAsp: 1.897 ± 0.038
1.426PheGlu: 1.426 ± 0.025
0.885PhePhe: 0.885 ± 0.024
2.988PheGly: 2.988 ± 0.05
0.623PheHis: 0.623 ± 0.019
0.679PheIle: 0.679 ± 0.019
0.43PheLys: 0.43 ± 0.017
2.53PheLeu: 2.53 ± 0.041
0.379PheMet: 0.379 ± 0.015
0.546PheAsn: 0.546 ± 0.016
1.425PhePro: 1.425 ± 0.03
0.638PheGln: 0.638 ± 0.02
1.942PheArg: 1.942 ± 0.031
1.442PheSer: 1.442 ± 0.025
1.989PheThr: 1.989 ± 0.033
2.071PheVal: 2.071 ± 0.037
0.431PheTrp: 0.431 ± 0.016
0.552PheTyr: 0.552 ± 0.019
0.0PheXaa: 0.0 ± 0.0
Gly
12.247GlyAla: 12.247 ± 0.097
0.902GlyCys: 0.902 ± 0.022
5.775GlyAsp: 5.775 ± 0.064
5.784GlyGlu: 5.784 ± 0.053
2.854GlyPhe: 2.854 ± 0.039
10.511GlyGly: 10.511 ± 0.106
2.45GlyHis: 2.45 ± 0.04
3.3GlyIle: 3.3 ± 0.043
2.316GlyLys: 2.316 ± 0.042
9.554GlyLeu: 9.554 ± 0.087
2.081GlyMet: 2.081 ± 0.035
1.847GlyAsn: 1.847 ± 0.035
6.394GlyPro: 6.394 ± 0.076
2.523GlyGln: 2.523 ± 0.038
8.995GlyArg: 8.995 ± 0.076
5.941GlySer: 5.941 ± 0.068
7.09GlyThr: 7.09 ± 0.074
7.395GlyVal: 7.395 ± 0.067
1.736GlyTrp: 1.736 ± 0.035
2.263GlyTyr: 2.263 ± 0.033
0.0GlyXaa: 0.0 ± 0.0
His
2.634HisAla: 2.634 ± 0.038
0.209HisCys: 0.209 ± 0.012
1.3HisAsp: 1.3 ± 0.026
1.2HisGlu: 1.2 ± 0.023
0.613HisPhe: 0.613 ± 0.017
2.574HisGly: 2.574 ± 0.037
0.675HisHis: 0.675 ± 0.018
0.666HisIle: 0.666 ± 0.017
0.299HisLys: 0.299 ± 0.013
2.291HisLeu: 2.291 ± 0.037
0.335HisMet: 0.335 ± 0.012
0.365HisAsn: 0.365 ± 0.014
1.797HisPro: 1.797 ± 0.033
0.59HisGln: 0.59 ± 0.016
2.275HisArg: 2.275 ± 0.035
0.978HisSer: 0.978 ± 0.023
1.341HisThr: 1.341 ± 0.026
1.557HisVal: 1.557 ± 0.032
0.366HisTrp: 0.366 ± 0.013
0.456HisTyr: 0.456 ± 0.014
0.0HisXaa: 0.0 ± 0.0
Ile
4.259IleAla: 4.259 ± 0.051
0.284IleCys: 0.284 ± 0.012
1.987IleAsp: 1.987 ± 0.036
1.789IleGlu: 1.789 ± 0.032
0.657IlePhe: 0.657 ± 0.019
3.366IleGly: 3.366 ± 0.05
0.527IleHis: 0.527 ± 0.015
0.732IleIle: 0.732 ± 0.024
0.635IleLys: 0.635 ± 0.019
2.148IleLeu: 2.148 ± 0.034
0.421IleMet: 0.421 ± 0.015
0.606IleAsn: 0.606 ± 0.02
1.643IlePro: 1.643 ± 0.029
0.593IleGln: 0.593 ± 0.017
2.192IleArg: 2.192 ± 0.029
1.5IleSer: 1.5 ± 0.027
1.99IleThr: 1.99 ± 0.036
2.433IleVal: 2.433 ± 0.041
0.351IleTrp: 0.351 ± 0.013
0.462IleTyr: 0.462 ± 0.015
0.0IleXaa: 0.0 ± 0.0
Lys
2.516LysAla: 2.516 ± 0.049
0.119LysCys: 0.119 ± 0.008
1.168LysAsp: 1.168 ± 0.03
1.129LysGlu: 1.129 ± 0.025
0.428LysPhe: 0.428 ± 0.015
1.623LysGly: 1.623 ± 0.037
0.412LysHis: 0.412 ± 0.015
0.76LysIle: 0.76 ± 0.023
0.792LysLys: 0.792 ± 0.033
1.924LysLeu: 1.924 ± 0.033
0.348LysMet: 0.348 ± 0.016
0.47LysAsn: 0.47 ± 0.016
1.298LysPro: 1.298 ± 0.031
0.66LysGln: 0.66 ± 0.024
1.391LysArg: 1.391 ± 0.028
1.046LysSer: 1.046 ± 0.024
1.165LysThr: 1.165 ± 0.026
1.627LysVal: 1.627 ± 0.033
0.235LysTrp: 0.235 ± 0.012
0.406LysTyr: 0.406 ± 0.016
0.0LysXaa: 0.0 ± 0.0
Leu
14.634LeuAla: 14.634 ± 0.104
0.853LeuCys: 0.853 ± 0.023
6.566LeuAsp: 6.566 ± 0.063
4.777LeuGlu: 4.777 ± 0.055
2.6LeuPhe: 2.6 ± 0.046
9.217LeuGly: 9.217 ± 0.085
2.253LeuHis: 2.253 ± 0.032
2.838LeuIle: 2.838 ± 0.047
1.719LeuLys: 1.719 ± 0.032
11.517LeuLeu: 11.517 ± 0.114
1.619LeuMet: 1.619 ± 0.032
1.529LeuAsn: 1.529 ± 0.03
6.619LeuPro: 6.619 ± 0.063
1.932LeuGln: 1.932 ± 0.036
9.126LeuArg: 9.126 ± 0.077
5.031LeuSer: 5.031 ± 0.051
6.361LeuThr: 6.361 ± 0.062
8.542LeuVal: 8.542 ± 0.083
1.294LeuTrp: 1.294 ± 0.026
1.834LeuTyr: 1.834 ± 0.034
0.0LeuXaa: 0.0 ± 0.0
Met
2.226MetAla: 2.226 ± 0.033
0.124MetCys: 0.124 ± 0.007
0.863MetAsp: 0.863 ± 0.02
0.775MetGlu: 0.775 ± 0.022
0.448MetPhe: 0.448 ± 0.015
1.216MetGly: 1.216 ± 0.03
0.322MetHis: 0.322 ± 0.013
0.621MetIle: 0.621 ± 0.018
0.386MetLys: 0.386 ± 0.014
1.655MetLeu: 1.655 ± 0.036
0.285MetMet: 0.285 ± 0.011
0.378MetAsn: 0.378 ± 0.013
1.129MetPro: 1.129 ± 0.024
0.401MetGln: 0.401 ± 0.012
1.413MetArg: 1.413 ± 0.026
1.236MetSer: 1.236 ± 0.023
1.47MetThr: 1.47 ± 0.027
1.229MetVal: 1.229 ± 0.023
0.204MetTrp: 0.204 ± 0.01
0.323MetTyr: 0.323 ± 0.013
0.0MetXaa: 0.0 ± 0.0
Asn
2.069AsnAla: 2.069 ± 0.033
0.161AsnCys: 0.161 ± 0.008
0.95AsnAsp: 0.95 ± 0.02
0.842AsnGlu: 0.842 ± 0.022
0.495AsnPhe: 0.495 ± 0.016
1.839AsnGly: 1.839 ± 0.04
0.404AsnHis: 0.404 ± 0.014
0.62AsnIle: 0.62 ± 0.019
0.372AsnLys: 0.372 ± 0.015
1.544AsnLeu: 1.544 ± 0.029
0.276AsnMet: 0.276 ± 0.015
0.411AsnAsn: 0.411 ± 0.016
1.301AsnPro: 1.301 ± 0.029
0.454AsnGln: 0.454 ± 0.015
1.253AsnArg: 1.253 ± 0.024
0.892AsnSer: 0.892 ± 0.022
1.063AsnThr: 1.063 ± 0.027
1.276AsnVal: 1.276 ± 0.03
0.293AsnTrp: 0.293 ± 0.011
0.408AsnTyr: 0.408 ± 0.014
0.0AsnXaa: 0.0 ± 0.0
Pro
9.713ProAla: 9.713 ± 0.077
0.365ProCys: 0.365 ± 0.015
4.837ProAsp: 4.837 ± 0.056
4.937ProGlu: 4.937 ± 0.061
1.504ProPhe: 1.504 ± 0.028
8.491ProGly: 8.491 ± 0.083
1.33ProHis: 1.33 ± 0.026
1.009ProIle: 1.009 ± 0.024
0.992ProLys: 0.992 ± 0.026
5.287ProLeu: 5.287 ± 0.063
0.916ProMet: 0.916 ± 0.021
0.799ProAsn: 0.799 ± 0.019
3.947ProPro: 3.947 ± 0.067
1.657ProGln: 1.657 ± 0.034
4.334ProArg: 4.334 ± 0.055
3.032ProSer: 3.032 ± 0.042
2.621ProThr: 2.621 ± 0.037
5.762ProVal: 5.762 ± 0.057
0.908ProTrp: 0.908 ± 0.023
1.456ProTyr: 1.456 ± 0.027
0.0ProXaa: 0.0 ± 0.0
Gln
3.27GlnAla: 3.27 ± 0.042
0.149GlnCys: 0.149 ± 0.01
1.298GlnAsp: 1.298 ± 0.028
1.423GlnGlu: 1.423 ± 0.029
0.638GlnPhe: 0.638 ± 0.019
2.117GlnGly: 2.117 ± 0.034
0.613GlnHis: 0.613 ± 0.02
0.959GlnIle: 0.959 ± 0.022
0.554GlnLys: 0.554 ± 0.018
3.005GlnLeu: 3.005 ± 0.043
0.425GlnMet: 0.425 ± 0.015
0.467GlnAsn: 0.467 ± 0.016
1.662GlnPro: 1.662 ± 0.034
1.271GlnGln: 1.271 ± 0.037
2.32GlnArg: 2.32 ± 0.035
1.178GlnSer: 1.178 ± 0.025
1.195GlnThr: 1.195 ± 0.023
2.069GlnVal: 2.069 ± 0.03
0.413GlnTrp: 0.413 ± 0.016
0.523GlnTyr: 0.523 ± 0.017
0.0GlnXaa: 0.0 ± 0.0
Arg
10.676ArgAla: 10.676 ± 0.104
0.638ArgCys: 0.638 ± 0.02
4.771ArgAsp: 4.771 ± 0.052
5.254ArgGlu: 5.254 ± 0.059
2.329ArgPhe: 2.329 ± 0.041
6.573ArgGly: 6.573 ± 0.066
2.219ArgHis: 2.219 ± 0.044
3.142ArgIle: 3.142 ± 0.044
1.695ArgLys: 1.695 ± 0.032
9.006ArgLeu: 9.006 ± 0.092
1.721ArgMet: 1.721 ± 0.032
1.424ArgAsn: 1.424 ± 0.028
5.578ArgPro: 5.578 ± 0.059
2.374ArgGln: 2.374 ± 0.036
8.555ArgArg: 8.555 ± 0.093
4.273ArgSer: 4.273 ± 0.05
5.558ArgThr: 5.558 ± 0.059
6.029ArgVal: 6.029 ± 0.058
1.38ArgTrp: 1.38 ± 0.03
1.843ArgTyr: 1.843 ± 0.028
0.0ArgXaa: 0.0 ± 0.0
Ser
6.733SerAla: 6.733 ± 0.063
0.423SerCys: 0.423 ± 0.016
2.637SerAsp: 2.637 ± 0.04
2.445SerGlu: 2.445 ± 0.045
1.543SerPhe: 1.543 ± 0.027
6.649SerGly: 6.649 ± 0.07
0.999SerHis: 0.999 ± 0.022
1.189SerIle: 1.189 ± 0.023
0.868SerLys: 0.868 ± 0.023
4.563SerLeu: 4.563 ± 0.05
0.999SerMet: 0.999 ± 0.022
0.803SerAsn: 0.803 ± 0.02
3.218SerPro: 3.218 ± 0.047
1.132SerGln: 1.132 ± 0.023
3.931SerArg: 3.931 ± 0.048
2.66SerSer: 2.66 ± 0.051
2.709SerThr: 2.709 ± 0.042
4.188SerVal: 4.188 ± 0.047
0.872SerTrp: 0.872 ± 0.022
1.178SerTyr: 1.178 ± 0.027
0.0SerXaa: 0.0 ± 0.0
Thr
8.985ThrAla: 8.985 ± 0.073
0.384ThrCys: 0.384 ± 0.015
3.542ThrAsp: 3.542 ± 0.041
3.437ThrGlu: 3.437 ± 0.043
1.494ThrPhe: 1.494 ± 0.03
7.56ThrGly: 7.56 ± 0.072
1.052ThrHis: 1.052 ± 0.021
1.44ThrIle: 1.44 ± 0.029
0.99ThrLys: 0.99 ± 0.024
5.265ThrLeu: 5.265 ± 0.051
0.865ThrMet: 0.865 ± 0.019
0.889ThrAsn: 0.889 ± 0.022
3.904ThrPro: 3.904 ± 0.05
1.122ThrGln: 1.122 ± 0.024
3.843ThrArg: 3.843 ± 0.048
2.811ThrSer: 2.811 ± 0.041
3.439ThrThr: 3.439 ± 0.05
6.004ThrVal: 6.004 ± 0.057
0.816ThrTrp: 0.816 ± 0.021
1.17ThrTyr: 1.17 ± 0.034
0.0ThrXaa: 0.0 ± 0.0
Val
10.207ValAla: 10.207 ± 0.086
0.769ValCys: 0.769 ± 0.02
4.638ValAsp: 4.638 ± 0.043
4.608ValGlu: 4.608 ± 0.061
2.449ValPhe: 2.449 ± 0.035
6.115ValGly: 6.115 ± 0.067
2.029ValHis: 2.029 ± 0.04
2.607ValIle: 2.607 ± 0.041
1.555ValLys: 1.555 ± 0.034
9.626ValLeu: 9.626 ± 0.075
1.378ValMet: 1.378 ± 0.028
1.62ValAsn: 1.62 ± 0.033
5.48ValPro: 5.48 ± 0.052
1.91ValGln: 1.91 ± 0.03
7.339ValArg: 7.339 ± 0.062
4.337ValSer: 4.337 ± 0.048
5.58ValThr: 5.58 ± 0.063
7.755ValVal: 7.755 ± 0.085
1.125ValTrp: 1.125 ± 0.026
1.538ValTyr: 1.538 ± 0.031
0.0ValXaa: 0.0 ± 0.0
Trp
1.614TrpAla: 1.614 ± 0.029
0.165TrpCys: 0.165 ± 0.009
0.849TrpAsp: 0.849 ± 0.024
0.746TrpGlu: 0.746 ± 0.017
0.509TrpPhe: 0.509 ± 0.017
1.033TrpGly: 1.033 ± 0.024
0.376TrpHis: 0.376 ± 0.014
0.504TrpIle: 0.504 ± 0.018
0.334TrpLys: 0.334 ± 0.013
1.721TrpLeu: 1.721 ± 0.032
0.277TrpMet: 0.277 ± 0.012
0.39TrpAsn: 0.39 ± 0.014
0.784TrpPro: 0.784 ± 0.022
0.625TrpGln: 0.625 ± 0.018
1.368TrpArg: 1.368 ± 0.027
0.929TrpSer: 0.929 ± 0.021
0.987TrpThr: 0.987 ± 0.024
0.9TrpVal: 0.9 ± 0.024
0.336TrpTrp: 0.336 ± 0.016
0.337TrpTyr: 0.337 ± 0.013
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.7TyrAla: 2.7 ± 0.036
0.183TyrCys: 0.183 ± 0.01
1.496TyrAsp: 1.496 ± 0.038
1.265TyrGlu: 1.265 ± 0.03
0.599TyrPhe: 0.599 ± 0.018
2.317TyrGly: 2.317 ± 0.033
0.372TyrHis: 0.372 ± 0.015
0.47TyrIle: 0.47 ± 0.016
0.357TyrLys: 0.357 ± 0.014
1.951TyrLeu: 1.951 ± 0.031
0.245TyrMet: 0.245 ± 0.011
0.391TyrAsn: 0.391 ± 0.015
0.985TyrPro: 0.985 ± 0.026
0.556TyrGln: 0.556 ± 0.019
1.865TyrArg: 1.865 ± 0.036
0.887TyrSer: 0.887 ± 0.02
1.157TyrThr: 1.157 ± 0.029
1.524TyrVal: 1.524 ± 0.029
0.357TyrTrp: 0.357 ± 0.014
0.447TyrTyr: 0.447 ± 0.019
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 6048 proteins (1998184 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski