Marinobacter profundi
Average proteome isoelectric point is 6.1
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3568 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2G1UJV0|A0A2G1UJV0_9ALTE AraC family transcriptional regulator OS=Marinobacter profundi OX=2666256 GN=CLH61_12760 PE=4 SV=1
MM1 pKa = 7.24 NNSLKK6 pKa = 10.53 FLTVTALAASLAACGGDD23 pKa = 3.6 GGDD26 pKa = 3.81 SQATGTLSLGITDD39 pKa = 4.49 APVDD43 pKa = 3.68 EE44 pKa = 4.9 LKK46 pKa = 10.73 EE47 pKa = 4.06 VNITVTGVEE56 pKa = 4.23 IKK58 pKa = 9.96 PASGEE63 pKa = 3.84 SRR65 pKa = 11.84 RR66 pKa = 11.84 YY67 pKa = 10.36 DD68 pKa = 3.47 FEE70 pKa = 4.63 EE71 pKa = 4.33 PLALDD76 pKa = 4.35 LLALQGGNAALLLNDD91 pKa = 3.94 EE92 pKa = 4.59 EE93 pKa = 4.91 VPAGEE98 pKa = 4.4 YY99 pKa = 10.09 NWVRR103 pKa = 11.84 LYY105 pKa = 10.65 ISTEE109 pKa = 3.87 PGEE112 pKa = 4.18 LTAVDD117 pKa = 4.14 MNGGVHH123 pKa = 7.26 DD124 pKa = 5.19 LYY126 pKa = 10.67 IPSGAQTGLKK136 pKa = 9.49 LVSGFTVPAGGDD148 pKa = 3.09 ADD150 pKa = 3.81 FTIDD154 pKa = 3.3 FDD156 pKa = 4.05 VRR158 pKa = 11.84 KK159 pKa = 10.36 SIVDD163 pKa = 3.59 PQGSNPSGAEE173 pKa = 3.9 YY174 pKa = 10.27 FLKK177 pKa = 10.55 PALRR181 pKa = 11.84 LIDD184 pKa = 3.71 NVEE187 pKa = 4.14 AGTISGTVDD196 pKa = 2.85 GTLIAEE202 pKa = 4.79 ACTDD206 pKa = 3.25 AATFDD211 pKa = 3.5 GGVYY215 pKa = 9.52 VHH217 pKa = 7.0 TGADD221 pKa = 3.61 QVPDD225 pKa = 4.05 DD226 pKa = 4.86 LGSTNEE232 pKa = 4.14 PLVIAPVAFDD242 pKa = 3.41 GTAYY246 pKa = 10.66 SYY248 pKa = 9.63 TAAFISAGNYY258 pKa = 6.41 TVSYY262 pKa = 9.8 TCDD265 pKa = 3.54 LDD267 pKa = 3.66 TRR269 pKa = 11.84 EE270 pKa = 4.4 DD271 pKa = 3.83 ANGEE275 pKa = 4.1 LVDD278 pKa = 5.06 EE279 pKa = 4.65 EE280 pKa = 4.49 LTFYY284 pKa = 9.3 GTQNVTVVADD294 pKa = 3.8 TTTTADD300 pKa = 3.39 FF301 pKa = 4.42
Molecular weight: 31.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.696
IPC2_protein 3.668
IPC_protein 3.694
Toseland 3.465
ProMoST 3.846
Dawson 3.681
Bjellqvist 3.834
Wikipedia 3.617
Rodwell 3.516
Grimsley 3.376
Solomon 3.668
Lehninger 3.63
Nozaki 3.795
DTASelect 4.037
Thurlkill 3.516
EMBOSS 3.63
Sillero 3.808
Patrickios 1.151
IPC_peptide 3.668
IPC2_peptide 3.783
IPC2.peptide.svr19 3.732
Protein with the highest isoelectric point:
>tr|A0A2G1ULI0|A0A2G1ULI0_9ALTE Branched-chain amino acid ABC transporter permease OS=Marinobacter profundi OX=2666256 GN=CLH61_09520 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.34 RR12 pKa = 11.84 KK13 pKa = 9.1 RR14 pKa = 11.84 VHH16 pKa = 6.26 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATANGRR28 pKa = 11.84 KK29 pKa = 8.93 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.42 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LCAA44 pKa = 3.92
Molecular weight: 5.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.423
IPC2_protein 10.906
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.13
Rodwell 12.34
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.076
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.092
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3568
0
3568
1209230
26
2800
338.9
37.24
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.489 ± 0.043
0.945 ± 0.012
5.748 ± 0.036
6.221 ± 0.042
3.72 ± 0.025
8.178 ± 0.042
2.207 ± 0.018
5.083 ± 0.031
3.188 ± 0.034
10.922 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.507 ± 0.019
3.037 ± 0.026
4.822 ± 0.024
3.91 ± 0.028
6.807 ± 0.041
5.606 ± 0.028
5.177 ± 0.03
7.457 ± 0.036
1.395 ± 0.016
2.583 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here