Streptococcus phage vB_SthS_VA460

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Moineauvirus; unclassified Moineauvirus

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2I6QQY9|A0A2I6QQY9_9CAUD Capsid protein OS=Streptococcus phage vB_SthS_VA460 OX=2069609 PE=4 SV=1
MM1 pKa = 7.3SVSKK5 pKa = 9.08EE6 pKa = 4.26TIMQTLNLDD15 pKa = 3.8EE16 pKa = 4.97TDD18 pKa = 3.78DD19 pKa = 3.8TALIPAYY26 pKa = 9.63IEE28 pKa = 4.23SAQQYY33 pKa = 9.35IINAVGSDD41 pKa = 3.33PKK43 pKa = 10.91FYY45 pKa = 10.87DD46 pKa = 3.46LDD48 pKa = 4.68SIEE51 pKa = 4.6SLYY54 pKa = 10.17DD55 pKa = 3.21TAVIALTSSYY65 pKa = 8.66FTYY68 pKa = 10.07RR69 pKa = 11.84VALTDD74 pKa = 3.37TATYY78 pKa = 9.41PVNLTLNSIIGQLRR92 pKa = 11.84GLYY95 pKa = 8.66ATYY98 pKa = 10.56SEE100 pKa = 4.46EE101 pKa = 5.06RR102 pKa = 11.84GDD104 pKa = 3.53

Molecular weight: 11.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2I6QR07|A0A2I6QR07_9CAUD Uncharacterized protein OS=Streptococcus phage vB_SthS_VA460 OX=2069609 PE=3 SV=1
MM1 pKa = 7.59RR2 pKa = 11.84VKK4 pKa = 10.41EE5 pKa = 3.78YY6 pKa = 11.09ALYY9 pKa = 10.36KK10 pKa = 10.21GEE12 pKa = 4.6EE13 pKa = 4.25IIGMGTKK20 pKa = 9.99RR21 pKa = 11.84EE22 pKa = 3.9IAEE25 pKa = 3.73QLGISVRR32 pKa = 11.84SVTFYY37 pKa = 9.93GTQSYY42 pKa = 11.12AKK44 pKa = 9.01RR45 pKa = 11.84TSEE48 pKa = 3.9KK49 pKa = 10.11NGRR52 pKa = 11.84RR53 pKa = 11.84LVKK56 pKa = 10.59LL57 pKa = 3.47

Molecular weight: 6.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

12631

40

1563

225.6

25.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.152 ± 0.425

0.776 ± 0.18

6.658 ± 0.216

6.666 ± 0.297

4.204 ± 0.155

6.88 ± 0.377

1.473 ± 0.1

6.634 ± 0.255

8.329 ± 0.431

7.561 ± 0.305

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.391 ± 0.184

6.532 ± 0.27

3.04 ± 0.175

3.974 ± 0.212

4.386 ± 0.256

6.365 ± 0.21

6.167 ± 0.338

6.017 ± 0.24

1.512 ± 0.131

4.283 ± 0.316
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski