Streptomyces sp. ADI97-07

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7272 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3N6IGG9|A0A3N6IGG9_9ACTN Aerobic cobaltochelatase subunit CobN OS=Streptomyces sp. ADI97-07 OX=1522762 GN=cobN PE=4 SV=1
MM1 pKa = 7.44SNEE4 pKa = 3.78EE5 pKa = 3.99NPEE8 pKa = 3.91QRR10 pKa = 11.84HH11 pKa = 5.22GQPDD15 pKa = 4.39PYY17 pKa = 10.64GGWEE21 pKa = 3.87PTPQGGEE28 pKa = 3.8YY29 pKa = 10.25DD30 pKa = 3.75AEE32 pKa = 4.03ATAFVHH38 pKa = 6.98LPPEE42 pKa = 4.36DD43 pKa = 3.66LANLGNDD50 pKa = 3.41PLAAPGHH57 pKa = 6.29GYY59 pKa = 9.16VPPMILPLTPAAGLDD74 pKa = 3.7PAATGSWVVQTQSQQEE90 pKa = 4.12RR91 pKa = 11.84AVQAGEE97 pKa = 4.36VPSEE101 pKa = 4.25VHH103 pKa = 5.98WPDD106 pKa = 4.31PNHH109 pKa = 6.12QDD111 pKa = 3.38PYY113 pKa = 10.42RR114 pKa = 11.84PEE116 pKa = 4.4PNHH119 pKa = 5.85QGSYY123 pKa = 6.76QQEE126 pKa = 4.67PYY128 pKa = 9.96RR129 pKa = 11.84QEE131 pKa = 4.34PYY133 pKa = 8.81GQEE136 pKa = 3.94YY137 pKa = 9.62PGAYY141 pKa = 9.02PSTPQYY147 pKa = 10.24EE148 pKa = 4.53QTSSTTAQWNFTEE161 pKa = 4.63SVPAAGPDD169 pKa = 3.86GPDD172 pKa = 3.21AASGSASASAEE183 pKa = 4.11PMGHH187 pKa = 5.9TGQWTIPVANGDD199 pKa = 3.76LPEE202 pKa = 4.38EE203 pKa = 4.44SGEE206 pKa = 4.15FAASALASPWYY217 pKa = 10.17ADD219 pKa = 3.97SPPATLPGGASAPWASQEE237 pKa = 3.93QALPEE242 pKa = 4.24APAPEE247 pKa = 4.43QSPEE251 pKa = 4.02PQPEE255 pKa = 3.92PAAEE259 pKa = 4.19SEE261 pKa = 4.64PEE263 pKa = 4.02LSPEE267 pKa = 4.24FPAEE271 pKa = 3.99LLPEE275 pKa = 4.74FPSEE279 pKa = 4.04PEE281 pKa = 3.8PVAEE285 pKa = 4.0AVVEE289 pKa = 4.11VSEE292 pKa = 4.2VSEE295 pKa = 4.23VSEE298 pKa = 4.66EE299 pKa = 4.02EE300 pKa = 4.2ASTGTAGPPGPLEE313 pKa = 4.03PLEE316 pKa = 4.72PLDD319 pKa = 4.12SAPAALDD326 pKa = 3.52VPDD329 pKa = 5.07ALDD332 pKa = 3.83VPDD335 pKa = 5.32APDD338 pKa = 3.54VPDD341 pKa = 4.76APDD344 pKa = 3.15VSDD347 pKa = 3.59VLGASGEE354 pKa = 4.05PAEE357 pKa = 4.55APEE360 pKa = 3.85LAAAFEE366 pKa = 4.74APLDD370 pKa = 3.69AHH372 pKa = 6.92GSSTPPGADD381 pKa = 3.55PAPEE385 pKa = 4.33GAPDD389 pKa = 3.67VPSEE393 pKa = 4.33HH394 pKa = 6.94PSVSYY399 pKa = 10.53VLHH402 pKa = 6.28VNGTDD407 pKa = 3.89RR408 pKa = 11.84PVSDD412 pKa = 2.97AWIGEE417 pKa = 4.06SLLYY421 pKa = 10.18VLRR424 pKa = 11.84EE425 pKa = 3.94RR426 pKa = 11.84LGLAGAKK433 pKa = 9.72DD434 pKa = 3.75GCSQGEE440 pKa = 4.12CGACNVQVDD449 pKa = 3.94GRR451 pKa = 11.84LVASCLVPAATTAGSEE467 pKa = 3.99VRR469 pKa = 11.84TVEE472 pKa = 3.9GLAVDD477 pKa = 4.82GEE479 pKa = 4.31PSDD482 pKa = 3.82VQRR485 pKa = 11.84ALARR489 pKa = 11.84CGAVQCGFCIPGMAMTVHH507 pKa = 7.14DD508 pKa = 5.06LLEE511 pKa = 4.83GNHH514 pKa = 6.43APSEE518 pKa = 4.33LEE520 pKa = 3.87TRR522 pKa = 11.84QALCGNLCRR531 pKa = 11.84CSGYY535 pKa = 10.39RR536 pKa = 11.84GVLDD540 pKa = 3.63AVRR543 pKa = 11.84EE544 pKa = 4.25VVDD547 pKa = 3.69GRR549 pKa = 11.84EE550 pKa = 3.77AAEE553 pKa = 4.32AEE555 pKa = 4.12NEE557 pKa = 4.2EE558 pKa = 5.07LRR560 pKa = 11.84IPHH563 pKa = 5.65QAGPGAGSVQPHH575 pKa = 5.47AHH577 pKa = 6.52EE578 pKa = 4.88GDD580 pKa = 3.55PRR582 pKa = 4.99

Molecular weight: 60.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3N6G1N6|A0A3N6G1N6_9ACTN Uncharacterized protein OS=Streptomyces sp. ADI97-07 OX=1522762 GN=EES45_14430 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILANRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.57GRR40 pKa = 11.84SSLSAA45 pKa = 3.62

Molecular weight: 5.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7272

0

7272

2347028

29

4813

322.7

34.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.5 ± 0.04

0.795 ± 0.008

6.005 ± 0.025

5.641 ± 0.028

2.691 ± 0.017

9.612 ± 0.032

2.31 ± 0.014

3.169 ± 0.018

2.126 ± 0.021

10.212 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.769 ± 0.012

1.753 ± 0.016

6.176 ± 0.029

2.752 ± 0.017

8.056 ± 0.033

5.272 ± 0.026

6.238 ± 0.024

8.379 ± 0.029

1.493 ± 0.013

2.052 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski