Amino acid dipepetide frequency for Streptomyces sp. ADI97-07

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.108AlaAla: 21.108 ± 0.157	1.05AlaCys: 1.05 ± 0.021	8.335AlaAsp: 8.335 ± 0.063	8.558AlaGlu: 8.558 ± 0.084	3.436AlaPhe: 3.436 ± 0.043	13.499AlaGly: 13.499 ± 0.105	2.859AlaHis: 2.859 ± 0.04	3.278AlaIle: 3.278 ± 0.045	2.711AlaLys: 2.711 ± 0.046	14.333AlaLeu: 14.333 ± 0.112	2.53AlaMet: 2.53 ± 0.033	1.848AlaAsn: 1.848 ± 0.032	7.486AlaPro: 7.486 ± 0.082	3.647AlaGln: 3.647 ± 0.041	10.14AlaArg: 10.14 ± 0.091	6.141AlaSer: 6.141 ± 0.054	7.02AlaThr: 7.02 ± 0.064	12.439AlaVal: 12.439 ± 0.096	1.83AlaTrp: 1.83 ± 0.032	2.749AlaTyr: 2.749 ± 0.038	0.0AlaXaa: 0.0 ± 0.0
Cys	1.086CysAla: 1.086 ± 0.023	0.096CysCys: 0.096 ± 0.007	0.464CysAsp: 0.464 ± 0.013	0.395CysGlu: 0.395 ± 0.013	0.232CysPhe: 0.232 ± 0.011	0.985CysGly: 0.985 ± 0.023	0.197CysHis: 0.197 ± 0.009	0.184CysIle: 0.184 ± 0.009	0.112CysLys: 0.112 ± 0.007	0.715CysLeu: 0.715 ± 0.018	0.13CysMet: 0.13 ± 0.008	0.138CysAsn: 0.138 ± 0.009	0.506CysPro: 0.506 ± 0.016	0.154CysGln: 0.154 ± 0.008	0.633CysArg: 0.633 ± 0.017	0.458CysSer: 0.458 ± 0.016	0.532CysThr: 0.532 ± 0.015	0.643CysVal: 0.643 ± 0.015	0.137CysTrp: 0.137 ± 0.007	0.151CysTyr: 0.151 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	7.687AspAla: 7.687 ± 0.059	0.421AspCys: 0.421 ± 0.016	3.607AspAsp: 3.607 ± 0.054	3.923AspGlu: 3.923 ± 0.043	1.66AspPhe: 1.66 ± 0.026	6.526AspGly: 6.526 ± 0.056	1.416AspHis: 1.416 ± 0.028	1.936AspIle: 1.936 ± 0.03	1.163AspLys: 1.163 ± 0.026	6.03AspLeu: 6.03 ± 0.06	0.838AspMet: 0.838 ± 0.021	0.971AspAsn: 0.971 ± 0.02	4.487AspPro: 4.487 ± 0.049	1.503AspGln: 1.503 ± 0.027	5.102AspArg: 5.102 ± 0.047	2.688AspSer: 2.688 ± 0.039	3.369AspThr: 3.369 ± 0.037	4.631AspVal: 4.631 ± 0.047	1.002AspTrp: 1.002 ± 0.021	1.091AspTyr: 1.091 ± 0.024	0.0AspXaa: 0.0 ± 0.0
Glu	7.395GluAla: 7.395 ± 0.073	0.372GluCys: 0.372 ± 0.013	2.946GluAsp: 2.946 ± 0.035	3.55GluGlu: 3.55 ± 0.048	1.358GluPhe: 1.358 ± 0.024	4.409GluGly: 4.409 ± 0.044	1.471GluHis: 1.471 ± 0.025	2.253GluIle: 2.253 ± 0.033	1.474GluLys: 1.474 ± 0.03	6.576GluLeu: 6.576 ± 0.063	0.901GluMet: 0.901 ± 0.023	1.031GluAsn: 1.031 ± 0.024	3.41GluPro: 3.41 ± 0.044	2.269GluGln: 2.269 ± 0.036	5.275GluArg: 5.275 ± 0.059	2.691GluSer: 2.691 ± 0.031	2.938GluThr: 2.938 ± 0.039	4.314GluVal: 4.314 ± 0.046	0.716GluTrp: 0.716 ± 0.02	1.06GluTyr: 1.06 ± 0.023	0.0GluXaa: 0.0 ± 0.0
Phe	3.599PheAla: 3.599 ± 0.047	0.26PheCys: 0.26 ± 0.012	1.946PheAsp: 1.946 ± 0.032	1.415PheGlu: 1.415 ± 0.027	0.866PhePhe: 0.866 ± 0.023	2.974PheGly: 2.974 ± 0.041	0.593PheHis: 0.593 ± 0.015	0.772PheIle: 0.772 ± 0.02	0.48PheLys: 0.48 ± 0.015	2.565PheLeu: 2.565 ± 0.035	0.419PheMet: 0.419 ± 0.014	0.556PheAsn: 0.556 ± 0.016	1.36PhePro: 1.36 ± 0.024	0.647PheGln: 0.647 ± 0.016	1.864PheArg: 1.864 ± 0.027	1.479PheSer: 1.479 ± 0.03	1.97PheThr: 1.97 ± 0.029	2.177PheVal: 2.177 ± 0.039	0.412PheTrp: 0.412 ± 0.013	0.554PheTyr: 0.554 ± 0.016	0.0PheXaa: 0.0 ± 0.0
Gly	11.501GlyAla: 11.501 ± 0.081	0.865GlyCys: 0.865 ± 0.019	5.221GlyAsp: 5.221 ± 0.054	5.103GlyGlu: 5.103 ± 0.055	2.872GlyPhe: 2.872 ± 0.039	9.09GlyGly: 9.09 ± 0.075	2.373GlyHis: 2.373 ± 0.035	3.505GlyIle: 3.505 ± 0.04	2.409GlyLys: 2.409 ± 0.039	9.52GlyLeu: 9.52 ± 0.081	1.987GlyMet: 1.987 ± 0.03	1.756GlyAsn: 1.756 ± 0.038	5.608GlyPro: 5.608 ± 0.067	2.64GlyGln: 2.64 ± 0.036	8.132GlyArg: 8.132 ± 0.075	5.77GlySer: 5.77 ± 0.066	6.623GlyThr: 6.623 ± 0.064	7.297GlyVal: 7.297 ± 0.062	1.648GlyTrp: 1.648 ± 0.028	2.204GlyTyr: 2.204 ± 0.038	0.0GlyXaa: 0.0 ± 0.0
His	2.69HisAla: 2.69 ± 0.036	0.227HisCys: 0.227 ± 0.011	1.361HisAsp: 1.361 ± 0.023	1.191HisGlu: 1.191 ± 0.024	0.645HisPhe: 0.645 ± 0.016	2.414HisGly: 2.414 ± 0.033	0.719HisHis: 0.719 ± 0.02	0.68HisIle: 0.68 ± 0.021	0.321HisLys: 0.321 ± 0.013	2.453HisLeu: 2.453 ± 0.037	0.34HisMet: 0.34 ± 0.012	0.38HisAsn: 0.38 ± 0.013	1.841HisPro: 1.841 ± 0.025	0.677HisGln: 0.677 ± 0.019	2.198HisArg: 2.198 ± 0.034	1.077HisSer: 1.077 ± 0.022	1.385HisThr: 1.385 ± 0.026	1.647HisVal: 1.647 ± 0.028	0.371HisTrp: 0.371 ± 0.012	0.484HisTyr: 0.484 ± 0.016	0.0HisXaa: 0.0 ± 0.0
Ile	4.755IleAla: 4.755 ± 0.052	0.305IleCys: 0.305 ± 0.012	2.272IleAsp: 2.272 ± 0.031	1.99IleGlu: 1.99 ± 0.031	0.703IlePhe: 0.703 ± 0.019	3.666IleGly: 3.666 ± 0.045	0.615IleHis: 0.615 ± 0.018	0.907IleIle: 0.907 ± 0.023	0.703IleLys: 0.703 ± 0.019	2.395IleLeu: 2.395 ± 0.035	0.433IleMet: 0.433 ± 0.015	0.691IleAsn: 0.691 ± 0.017	1.787IlePro: 1.787 ± 0.027	0.767IleGln: 0.767 ± 0.019	2.236IleArg: 2.236 ± 0.029	1.7IleSer: 1.7 ± 0.028	2.256IleThr: 2.256 ± 0.032	2.62IleVal: 2.62 ± 0.038	0.375IleTrp: 0.375 ± 0.012	0.519IleTyr: 0.519 ± 0.015	0.0IleXaa: 0.0 ± 0.0
Lys	2.832LysAla: 2.832 ± 0.049	0.11LysCys: 0.11 ± 0.007	1.346LysAsp: 1.346 ± 0.031	1.203LysGlu: 1.203 ± 0.029	0.464LysPhe: 0.464 ± 0.015	1.793LysGly: 1.793 ± 0.033	0.439LysHis: 0.439 ± 0.014	0.835LysIle: 0.835 ± 0.018	0.895LysLys: 0.895 ± 0.028	1.993LysLeu: 1.993 ± 0.037	0.39LysMet: 0.39 ± 0.013	0.55LysAsn: 0.55 ± 0.016	1.349LysPro: 1.349 ± 0.027	0.715LysGln: 0.715 ± 0.02	1.412LysArg: 1.412 ± 0.03	1.176LysSer: 1.176 ± 0.028	1.223LysThr: 1.223 ± 0.028	1.855LysVal: 1.855 ± 0.037	0.263LysTrp: 0.263 ± 0.011	0.419LysTyr: 0.419 ± 0.014	0.0LysXaa: 0.0 ± 0.0
Leu	14.523LeuAla: 14.523 ± 0.116	0.861LeuCys: 0.861 ± 0.021	6.666LeuAsp: 6.666 ± 0.059	4.579LeuGlu: 4.579 ± 0.055	2.574LeuPhe: 2.574 ± 0.033	9.208LeuGly: 9.208 ± 0.076	2.357LeuHis: 2.357 ± 0.035	3.3LeuIle: 3.3 ± 0.042	1.934LeuLys: 1.934 ± 0.034	11.269LeuLeu: 11.269 ± 0.109	1.709LeuMet: 1.709 ± 0.026	1.663LeuAsn: 1.663 ± 0.029	6.516LeuPro: 6.516 ± 0.062	2.211LeuGln: 2.211 ± 0.036	8.615LeuArg: 8.615 ± 0.072	5.456LeuSer: 5.456 ± 0.048	6.837LeuThr: 6.837 ± 0.058	8.768LeuVal: 8.768 ± 0.075	1.259LeuTrp: 1.259 ± 0.024	1.816LeuTyr: 1.816 ± 0.027	0.0LeuXaa: 0.0 ± 0.0
Met	2.249MetAla: 2.249 ± 0.033	0.14MetCys: 0.14 ± 0.008	0.902MetAsp: 0.902 ± 0.019	0.808MetGlu: 0.808 ± 0.02	0.447MetPhe: 0.447 ± 0.014	1.337MetGly: 1.337 ± 0.026	0.387MetHis: 0.387 ± 0.012	0.676MetIle: 0.676 ± 0.017	0.435MetLys: 0.435 ± 0.013	1.727MetLeu: 1.727 ± 0.03	0.309MetMet: 0.309 ± 0.013	0.435MetAsn: 0.435 ± 0.013	1.16MetPro: 1.16 ± 0.023	0.457MetGln: 0.457 ± 0.014	1.459MetArg: 1.459 ± 0.025	1.403MetSer: 1.403 ± 0.024	1.555MetThr: 1.555 ± 0.029	1.262MetVal: 1.262 ± 0.021	0.206MetTrp: 0.206 ± 0.009	0.332MetTyr: 0.332 ± 0.012	0.0MetXaa: 0.0 ± 0.0
Asn	2.174AsnAla: 2.174 ± 0.034	0.162AsnCys: 0.162 ± 0.009	0.99AsnAsp: 0.99 ± 0.02	0.833AsnGlu: 0.833 ± 0.019	0.473AsnPhe: 0.473 ± 0.013	1.896AsnGly: 1.896 ± 0.036	0.384AsnHis: 0.384 ± 0.014	0.655AsnIle: 0.655 ± 0.018	0.404AsnLys: 0.404 ± 0.016	1.608AsnLeu: 1.608 ± 0.026	0.313AsnMet: 0.313 ± 0.01	0.42AsnAsn: 0.42 ± 0.017	1.308AsnPro: 1.308 ± 0.026	0.511AsnGln: 0.511 ± 0.016	1.305AsnArg: 1.305 ± 0.024	0.95AsnSer: 0.95 ± 0.026	1.156AsnThr: 1.156 ± 0.028	1.305AsnVal: 1.305 ± 0.027	0.279AsnTrp: 0.279 ± 0.012	0.403AsnTyr: 0.403 ± 0.014	0.0AsnXaa: 0.0 ± 0.0
Pro	9.047ProAla: 9.047 ± 0.088	0.367ProCys: 0.367 ± 0.014	4.498ProAsp: 4.498 ± 0.051	4.305ProGlu: 4.305 ± 0.049	1.537ProPhe: 1.537 ± 0.025	7.045ProGly: 7.045 ± 0.057	1.416ProHis: 1.416 ± 0.026	1.172ProIle: 1.172 ± 0.021	1.152ProLys: 1.152 ± 0.027	5.414ProLeu: 5.414 ± 0.059	1.028ProMet: 1.028 ± 0.022	0.851ProAsn: 0.851 ± 0.022	3.603ProPro: 3.603 ± 0.066	1.818ProGln: 1.818 ± 0.037	4.006ProArg: 4.006 ± 0.05	3.347ProSer: 3.347 ± 0.044	3.098ProThr: 3.098 ± 0.042	5.746ProVal: 5.746 ± 0.053	0.89ProTrp: 0.89 ± 0.023	1.421ProTyr: 1.421 ± 0.027	0.0ProXaa: 0.0 ± 0.0
Gln	3.528GlnAla: 3.528 ± 0.044	0.169GlnCys: 0.169 ± 0.009	1.467GlnAsp: 1.467 ± 0.028	1.515GlnGlu: 1.515 ± 0.028	0.704GlnPhe: 0.704 ± 0.017	2.257GlnGly: 2.257 ± 0.038	0.72GlnHis: 0.72 ± 0.018	1.077GlnIle: 1.077 ± 0.023	0.644GlnLys: 0.644 ± 0.019	3.07GlnLeu: 3.07 ± 0.041	0.492GlnMet: 0.492 ± 0.015	0.536GlnAsn: 0.536 ± 0.015	1.694GlnPro: 1.694 ± 0.031	1.297GlnGln: 1.297 ± 0.039	2.359GlnArg: 2.359 ± 0.036	1.292GlnSer: 1.292 ± 0.024	1.297GlnThr: 1.297 ± 0.022	2.346GlnVal: 2.346 ± 0.037	0.471GlnTrp: 0.471 ± 0.015	0.588GlnTyr: 0.588 ± 0.017	0.0GlnXaa: 0.0 ± 0.0
Arg	9.864ArgAla: 9.864 ± 0.097	0.615ArgCys: 0.615 ± 0.018	4.226ArgAsp: 4.226 ± 0.05	4.515ArgGlu: 4.515 ± 0.053	2.257ArgPhe: 2.257 ± 0.037	5.982ArgGly: 5.982 ± 0.056	2.095ArgHis: 2.095 ± 0.034	3.413ArgIle: 3.413 ± 0.038	1.712ArgLys: 1.712 ± 0.033	8.502ArgLeu: 8.502 ± 0.075	1.756ArgMet: 1.756 ± 0.027	1.435ArgAsn: 1.435 ± 0.025	5.056ArgPro: 5.056 ± 0.053	2.291ArgGln: 2.291 ± 0.036	7.685ArgArg: 7.685 ± 0.083	4.419ArgSer: 4.419 ± 0.045	5.885ArgThr: 5.885 ± 0.053	5.697ArgVal: 5.697 ± 0.052	1.382ArgTrp: 1.382 ± 0.023	1.769ArgTyr: 1.769 ± 0.031	0.0ArgXaa: 0.0 ± 0.0
Ser	7.042SerAla: 7.042 ± 0.06	0.432SerCys: 0.432 ± 0.015	2.864SerAsp: 2.864 ± 0.041	2.551SerGlu: 2.551 ± 0.034	1.582SerPhe: 1.582 ± 0.027	6.219SerGly: 6.219 ± 0.064	1.078SerHis: 1.078 ± 0.022	1.484SerIle: 1.484 ± 0.029	1.067SerLys: 1.067 ± 0.026	4.926SerLeu: 4.926 ± 0.056	1.118SerMet: 1.118 ± 0.024	0.867SerAsn: 0.867 ± 0.022	3.326SerPro: 3.326 ± 0.048	1.249SerGln: 1.249 ± 0.021	3.838SerArg: 3.838 ± 0.044	3.075SerSer: 3.075 ± 0.04	3.284SerThr: 3.284 ± 0.048	4.527SerVal: 4.527 ± 0.042	0.955SerTrp: 0.955 ± 0.021	1.236SerTyr: 1.236 ± 0.026	0.0SerXaa: 0.0 ± 0.0
Thr	9.13ThrAla: 9.13 ± 0.069	0.422ThrCys: 0.422 ± 0.014	3.73ThrAsp: 3.73 ± 0.04	3.36ThrGlu: 3.36 ± 0.038	1.638ThrPhe: 1.638 ± 0.029	7.125ThrGly: 7.125 ± 0.057	1.219ThrHis: 1.219 ± 0.022	1.653ThrIle: 1.653 ± 0.029	1.164ThrLys: 1.164 ± 0.033	5.748ThrLeu: 5.748 ± 0.054	0.968ThrMet: 0.968 ± 0.023	0.987ThrAsn: 0.987 ± 0.023	4.053ThrPro: 4.053 ± 0.038	1.368ThrGln: 1.368 ± 0.025	4.026ThrArg: 4.026 ± 0.049	3.269ThrSer: 3.269 ± 0.043	4.009ThrThr: 4.009 ± 0.051	6.178ThrVal: 6.178 ± 0.055	0.935ThrTrp: 0.935 ± 0.021	1.398ThrTyr: 1.398 ± 0.031	0.0ThrXaa: 0.0 ± 0.0
Val	10.374ValAla: 10.374 ± 0.077	0.752ValCys: 0.752 ± 0.016	4.884ValAsp: 4.884 ± 0.053	4.597ValGlu: 4.597 ± 0.048	2.366ValPhe: 2.366 ± 0.037	6.372ValGly: 6.372 ± 0.057	2.035ValHis: 2.035 ± 0.029	2.878ValIle: 2.878 ± 0.036	1.732ValLys: 1.732 ± 0.036	9.539ValLeu: 9.539 ± 0.078	1.487ValMet: 1.487 ± 0.028	1.6ValAsn: 1.6 ± 0.029	5.409ValPro: 5.409 ± 0.057	2.095ValGln: 2.095 ± 0.029	7.173ValArg: 7.173 ± 0.064	4.425ValSer: 4.425 ± 0.046	5.669ValThr: 5.669 ± 0.059	7.74ValVal: 7.74 ± 0.078	1.125ValTrp: 1.125 ± 0.022	1.534ValTyr: 1.534 ± 0.024	0.0ValXaa: 0.0 ± 0.0
Trp	1.654TrpAla: 1.654 ± 0.031	0.147TrpCys: 0.147 ± 0.007	0.842TrpAsp: 0.842 ± 0.022	0.745TrpGlu: 0.745 ± 0.019	0.479TrpPhe: 0.479 ± 0.014	1.058TrpGly: 1.058 ± 0.023	0.363TrpHis: 0.363 ± 0.012	0.555TrpIle: 0.555 ± 0.017	0.381TrpLys: 0.381 ± 0.014	1.688TrpLeu: 1.688 ± 0.031	0.306TrpMet: 0.306 ± 0.012	0.4TrpAsn: 0.4 ± 0.013	0.767TrpPro: 0.767 ± 0.02	0.635TrpGln: 0.635 ± 0.017	1.262TrpArg: 1.262 ± 0.028	0.941TrpSer: 0.941 ± 0.024	1.042TrpThr: 1.042 ± 0.022	0.981TrpVal: 0.981 ± 0.019	0.341TrpTrp: 0.341 ± 0.012	0.338TrpTyr: 0.338 ± 0.012	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.76TyrAla: 2.76 ± 0.033	0.175TyrCys: 0.175 ± 0.008	1.484TyrAsp: 1.484 ± 0.035	1.273TyrGlu: 1.273 ± 0.025	0.614TyrPhe: 0.614 ± 0.015	2.268TyrGly: 2.268 ± 0.035	0.365TyrHis: 0.365 ± 0.013	0.481TyrIle: 0.481 ± 0.015	0.369TyrLys: 0.369 ± 0.012	2.05TyrLeu: 2.05 ± 0.035	0.23TyrMet: 0.23 ± 0.01	0.417TyrAsn: 0.417 ± 0.014	1.034TyrPro: 1.034 ± 0.022	0.573TyrGln: 0.573 ± 0.017	1.836TyrArg: 1.836 ± 0.03	0.962TyrSer: 0.962 ± 0.023	1.231TyrThr: 1.231 ± 0.028	1.614TyrVal: 1.614 ± 0.03	0.329TyrTrp: 0.329 ± 0.013	0.452TyrTyr: 0.452 ± 0.016	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 7272 proteins (2347028 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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