Sedimenticola thiotaurini
Average proteome isoelectric point is 6.18
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3407 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0F7JZW3|A0A0F7JZW3_9GAMM ATP-binding protein OS=Sedimenticola thiotaurini OX=1543721 GN=AAY24_06950 PE=3 SV=1
MM1 pKa = 7.62 KK2 pKa = 10.43 KK3 pKa = 9.88 HH4 pKa = 6.08 FLNAAIISLLTANSVQADD22 pKa = 3.75 NLADD26 pKa = 3.61 AFSNGTVSGDD36 pKa = 3.14 LNLYY40 pKa = 8.88 YY41 pKa = 10.83 KK42 pKa = 10.86 NIDD45 pKa = 3.43 SRR47 pKa = 11.84 VGADD51 pKa = 3.02 AGYY54 pKa = 8.24 STGNLGLKK62 pKa = 10.43 YY63 pKa = 8.95 EE64 pKa = 4.19 TAPLNGLQLGLAFRR78 pKa = 11.84 HH79 pKa = 5.69 GSEE82 pKa = 4.11 VDD84 pKa = 3.04 EE85 pKa = 5.35 DD86 pKa = 4.12 NPGDD90 pKa = 3.65 SDD92 pKa = 3.83 EE93 pKa = 4.96 GVGSLLTEE101 pKa = 4.86 AYY103 pKa = 9.38 ISYY106 pKa = 10.45 QGDD109 pKa = 3.39 GFGAVLGRR117 pKa = 11.84 QAIDD121 pKa = 3.74 LEE123 pKa = 4.79 WIGDD127 pKa = 3.58 YY128 pKa = 11.11 HH129 pKa = 6.58 EE130 pKa = 5.69 AVTLTSDD137 pKa = 4.48 LLPATEE143 pKa = 4.14 LTVGYY148 pKa = 9.68 SRR150 pKa = 11.84 SIAVADD156 pKa = 4.15 EE157 pKa = 5.33 DD158 pKa = 4.83 DD159 pKa = 4.41 LQHH162 pKa = 6.66 SFTDD166 pKa = 3.56 IGDD169 pKa = 4.04 DD170 pKa = 4.04 GAWFLDD176 pKa = 3.64 ASINPAEE183 pKa = 4.41 GLTLNPYY190 pKa = 9.49 YY191 pKa = 10.15 IHH193 pKa = 7.19 EE194 pKa = 4.89 DD195 pKa = 3.55 EE196 pKa = 4.88 VFDD199 pKa = 4.9 GLGLKK204 pKa = 10.17 ADD206 pKa = 4.4 FEE208 pKa = 4.77 HH209 pKa = 7.65 ASGLGMTAHH218 pKa = 6.88 YY219 pKa = 7.5 ATSDD223 pKa = 3.17 VDD225 pKa = 3.83 VAGEE229 pKa = 3.88 SDD231 pKa = 3.73 GAILHH236 pKa = 6.31 LEE238 pKa = 4.01 LRR240 pKa = 11.84 GTLGGLALAAGYY252 pKa = 10.14 IDD254 pKa = 4.61 TDD256 pKa = 3.6 DD257 pKa = 3.58 QGGVGHH263 pKa = 7.6 LDD265 pKa = 3.5 DD266 pKa = 5.96 LGDD269 pKa = 4.17 NISPLEE275 pKa = 4.42 EE276 pKa = 4.41 GNQVYY281 pKa = 10.33 GPDD284 pKa = 3.37 AQTWYY289 pKa = 11.01 LGGEE293 pKa = 4.1 YY294 pKa = 10.25 SIGRR298 pKa = 11.84 ATIGALVGNTDD309 pKa = 3.35 YY310 pKa = 11.55 AGNSEE315 pKa = 4.13 EE316 pKa = 4.7 QEE318 pKa = 3.85 INLILGYY325 pKa = 10.65 DD326 pKa = 4.27 LAAVAEE332 pKa = 4.27 NLSVSLVYY340 pKa = 10.89 ADD342 pKa = 4.09 IDD344 pKa = 3.89 AEE346 pKa = 4.33 AGNDD350 pKa = 3.73 DD351 pKa = 4.2 YY352 pKa = 12.08 EE353 pKa = 5.17 KK354 pKa = 10.14 LTLMLSYY361 pKa = 11.17 GFF363 pKa = 4.49
Molecular weight: 38.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.756
IPC2_protein 3.719
IPC_protein 3.745
Toseland 3.528
ProMoST 3.91
Dawson 3.745
Bjellqvist 3.897
Wikipedia 3.681
Rodwell 3.567
Grimsley 3.427
Solomon 3.732
Lehninger 3.694
Nozaki 3.846
DTASelect 4.101
Thurlkill 3.579
EMBOSS 3.681
Sillero 3.872
Patrickios 0.502
IPC_peptide 3.732
IPC2_peptide 3.846
IPC2.peptide.svr19 3.766
Protein with the highest isoelectric point:
>tr|A0A0F7K1A9|A0A0F7K1A9_9GAMM Rod shape-determining protein MreD OS=Sedimenticola thiotaurini OX=1543721 GN=AAY24_14300 PE=3 SV=1
MM1 pKa = 7.4 AVKK4 pKa = 10.25 KK5 pKa = 10.6 ASKK8 pKa = 10.27 KK9 pKa = 9.75 KK10 pKa = 9.37 AAKK13 pKa = 9.67 KK14 pKa = 10.11 KK15 pKa = 9.84 SAAAKK20 pKa = 8.13 KK21 pKa = 6.01 TTSKK25 pKa = 10.87 KK26 pKa = 10.51 AVTPKK31 pKa = 10.68 KK32 pKa = 10.64 SISKK36 pKa = 10.12 KK37 pKa = 9.81 KK38 pKa = 10.58 AVTKK42 pKa = 10.47 KK43 pKa = 10.39 KK44 pKa = 10.41 AVAKK48 pKa = 10.54 KK49 pKa = 9.82 KK50 pKa = 8.84 VAKK53 pKa = 10.49 KK54 pKa = 10.31 KK55 pKa = 7.5 VTKK58 pKa = 10.24 KK59 pKa = 10.29 KK60 pKa = 10.04 SAAKK64 pKa = 10.09 KK65 pKa = 9.85 KK66 pKa = 9.21 VVKK69 pKa = 10.62 KK70 pKa = 10.42 KK71 pKa = 9.03 VAKK74 pKa = 10.19 KK75 pKa = 9.9 KK76 pKa = 10.01 AAAKK80 pKa = 10.15 KK81 pKa = 10.02 KK82 pKa = 8.84 VVKK85 pKa = 10.61 KK86 pKa = 10.42 KK87 pKa = 9.79 VSKK90 pKa = 10.73 KK91 pKa = 8.64 KK92 pKa = 8.1 TAAKK96 pKa = 10.28 KK97 pKa = 10.37 KK98 pKa = 7.15 VTKK101 pKa = 10.41 KK102 pKa = 10.25 KK103 pKa = 10.19 AAPKK107 pKa = 10.18 QKK109 pKa = 9.62 IAKK112 pKa = 9.72 KK113 pKa = 9.97 SASKK117 pKa = 10.64 KK118 pKa = 9.75 KK119 pKa = 9.06 ATAKK123 pKa = 10.64 KK124 pKa = 10.26 KK125 pKa = 7.52 VTKK128 pKa = 10.18 KK129 pKa = 10.21 KK130 pKa = 10.07 AALRR134 pKa = 11.84 PRR136 pKa = 11.84 KK137 pKa = 9.57 VRR139 pKa = 11.84 PRR141 pKa = 11.84 KK142 pKa = 9.7 KK143 pKa = 9.93 ATTKK147 pKa = 10.43 RR148 pKa = 3.49
Molecular weight: 16.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.356
IPC2_protein 11.301
IPC_protein 12.457
Toseland 12.793
ProMoST 13.13
Dawson 12.822
Bjellqvist 12.691
Wikipedia 13.173
Rodwell 13.364
Grimsley 12.837
Solomon 13.188
Lehninger 13.1
Nozaki 12.778
DTASelect 12.691
Thurlkill 12.778
EMBOSS 13.247
Sillero 12.778
Patrickios 13.071
IPC_peptide 13.188
IPC2_peptide 12.106
IPC2.peptide.svr19 8.8
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3407
0
3407
1070962
51
1955
314.3
34.77
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.575 ± 0.045
1.033 ± 0.015
5.558 ± 0.031
6.408 ± 0.041
3.643 ± 0.026
7.772 ± 0.043
2.222 ± 0.02
5.749 ± 0.03
3.754 ± 0.04
11.196 ± 0.062
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.612 ± 0.021
3.15 ± 0.025
4.755 ± 0.029
4.468 ± 0.036
6.534 ± 0.04
5.734 ± 0.028
4.95 ± 0.021
6.948 ± 0.038
1.252 ± 0.017
2.688 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here