Bacillus sp. 7504-2

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; unclassified Bacillus (in: Bacteria)

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4463 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A268IJV3|A0A268IJV3_9BACI Uncharacterized protein OS=Bacillus sp. 7504-2 OX=2022547 GN=CHH80_01645 PE=4 SV=1
MM1 pKa = 7.74KK2 pKa = 9.98EE3 pKa = 3.69PSSFGKK9 pKa = 10.28DD10 pKa = 3.21YY11 pKa = 10.93IYY13 pKa = 10.1LTEE16 pKa = 4.15VSALEE21 pKa = 4.68DD22 pKa = 3.94DD23 pKa = 3.6SDD25 pKa = 4.95FYY27 pKa = 12.07VEE29 pKa = 4.8LYY31 pKa = 10.03PYY33 pKa = 10.47RR34 pKa = 11.84PDD36 pKa = 3.77LQCSGSPIKK45 pKa = 10.47VLHH48 pKa = 6.31NFDD51 pKa = 3.99EE52 pKa = 5.82LIDD55 pKa = 3.95FLEE58 pKa = 4.23NLL60 pKa = 4.03

Molecular weight: 7.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A268I8C5|A0A268I8C5_9BACI YIP1 family protein OS=Bacillus sp. 7504-2 OX=2022547 GN=CHH80_22245 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84KK11 pKa = 9.27RR12 pKa = 11.84SKK14 pKa = 9.34VHH16 pKa = 5.82GFRR19 pKa = 11.84SRR21 pKa = 11.84MSSANGRR28 pKa = 11.84KK29 pKa = 8.82VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.05GRR39 pKa = 11.84KK40 pKa = 8.7VLSAA44 pKa = 4.05

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4463

0

4463

1284409

26

1719

287.8

32.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.96 ± 0.04

0.753 ± 0.011

4.816 ± 0.027

7.859 ± 0.045

4.67 ± 0.033

6.752 ± 0.032

2.082 ± 0.017

8.185 ± 0.035

7.015 ± 0.037

10.002 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.738 ± 0.015

4.452 ± 0.025

3.486 ± 0.02

3.681 ± 0.025

4.065 ± 0.024

5.888 ± 0.024

5.248 ± 0.023

6.852 ± 0.027

0.989 ± 0.013

3.507 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski