Amino acid dipepetide frequency for Bacillus sp. 7504-2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.635AlaAla: 5.635 ± 0.096
0.644AlaCys: 0.644 ± 0.026
3.318AlaAsp: 3.318 ± 0.063
4.737AlaGlu: 4.737 ± 0.075
3.265AlaPhe: 3.265 ± 0.05
5.093AlaGly: 5.093 ± 0.076
1.282AlaHis: 1.282 ± 0.038
6.107AlaIle: 6.107 ± 0.087
4.878AlaLys: 4.878 ± 0.069
7.424AlaLeu: 7.424 ± 0.084
1.945AlaMet: 1.945 ± 0.04
2.885AlaAsn: 2.885 ± 0.049
2.02AlaPro: 2.02 ± 0.048
2.263AlaGln: 2.263 ± 0.047
2.635AlaArg: 2.635 ± 0.051
3.946AlaSer: 3.946 ± 0.055
3.497AlaThr: 3.497 ± 0.062
5.277AlaVal: 5.277 ± 0.07
0.568AlaTrp: 0.568 ± 0.025
2.182AlaTyr: 2.182 ± 0.045
0.0AlaXaa: 0.0 ± 0.0
Cys
0.442CysAla: 0.442 ± 0.02
0.101CysCys: 0.101 ± 0.009
0.381CysAsp: 0.381 ± 0.016
0.504CysGlu: 0.504 ± 0.021
0.322CysPhe: 0.322 ± 0.015
0.7CysGly: 0.7 ± 0.031
0.234CysHis: 0.234 ± 0.013
0.541CysIle: 0.541 ± 0.019
0.42CysLys: 0.42 ± 0.018
0.719CysLeu: 0.719 ± 0.028
0.174CysMet: 0.174 ± 0.012
0.308CysAsn: 0.308 ± 0.015
0.402CysPro: 0.402 ± 0.018
0.291CysGln: 0.291 ± 0.015
0.309CysArg: 0.309 ± 0.017
0.518CysSer: 0.518 ± 0.021
0.378CysThr: 0.378 ± 0.016
0.431CysVal: 0.431 ± 0.021
0.076CysTrp: 0.076 ± 0.007
0.28CysTyr: 0.28 ± 0.016
0.0CysXaa: 0.0 ± 0.0
Asp
3.002AspAla: 3.002 ± 0.052
0.379AspCys: 0.379 ± 0.017
2.421AspAsp: 2.421 ± 0.045
4.484AspGlu: 4.484 ± 0.07
2.488AspPhe: 2.488 ± 0.048
3.263AspGly: 3.263 ± 0.059
1.035AspHis: 1.035 ± 0.03
4.103AspIle: 4.103 ± 0.056
2.906AspLys: 2.906 ± 0.054
4.76AspLeu: 4.76 ± 0.063
1.25AspMet: 1.25 ± 0.031
1.7AspAsn: 1.7 ± 0.044
1.897AspPro: 1.897 ± 0.039
1.609AspGln: 1.609 ± 0.034
1.967AspArg: 1.967 ± 0.038
2.56AspSer: 2.56 ± 0.047
2.105AspThr: 2.105 ± 0.043
3.567AspVal: 3.567 ± 0.053
0.585AspTrp: 0.585 ± 0.022
2.081AspTyr: 2.081 ± 0.046
0.0AspXaa: 0.0 ± 0.0
Glu
5.474GluAla: 5.474 ± 0.069
0.426GluCys: 0.426 ± 0.018
3.727GluAsp: 3.727 ± 0.053
7.389GluGlu: 7.389 ± 0.095
2.693GluPhe: 2.693 ± 0.044
4.416GluGly: 4.416 ± 0.061
1.653GluHis: 1.653 ± 0.035
6.3GluIle: 6.3 ± 0.086
7.056GluLys: 7.056 ± 0.082
7.691GluLeu: 7.691 ± 0.087
2.449GluMet: 2.449 ± 0.044
3.989GluAsn: 3.989 ± 0.06
2.084GluPro: 2.084 ± 0.045
3.759GluGln: 3.759 ± 0.06
3.841GluArg: 3.841 ± 0.056
3.479GluSer: 3.479 ± 0.056
3.772GluThr: 3.772 ± 0.057
5.231GluVal: 5.231 ± 0.066
0.836GluTrp: 0.836 ± 0.027
2.322GluTyr: 2.322 ± 0.042
0.0GluXaa: 0.0 ± 0.0
Phe
3.187PheAla: 3.187 ± 0.057
0.374PheCys: 0.374 ± 0.019
2.214PheAsp: 2.214 ± 0.045
3.017PheGlu: 3.017 ± 0.051
2.562PhePhe: 2.562 ± 0.053
3.249PheGly: 3.249 ± 0.062
1.05PheHis: 1.05 ± 0.03
4.087PheIle: 4.087 ± 0.069
2.516PheLys: 2.516 ± 0.049
4.899PheLeu: 4.899 ± 0.078
1.176PheMet: 1.176 ± 0.033
1.928PheAsn: 1.928 ± 0.036
1.706PhePro: 1.706 ± 0.042
1.801PheGln: 1.801 ± 0.042
1.561PheArg: 1.561 ± 0.041
3.365PheSer: 3.365 ± 0.057
2.581PheThr: 2.581 ± 0.053
3.24PheVal: 3.24 ± 0.061
0.467PheTrp: 0.467 ± 0.02
1.72PheTyr: 1.72 ± 0.039
0.0PheXaa: 0.0 ± 0.0
Gly
4.907GlyAla: 4.907 ± 0.08
0.626GlyCys: 0.626 ± 0.025
3.012GlyAsp: 3.012 ± 0.053
4.715GlyGlu: 4.715 ± 0.07
3.382GlyPhe: 3.382 ± 0.059
4.634GlyGly: 4.634 ± 0.078
1.32GlyHis: 1.32 ± 0.031
6.095GlyIle: 6.095 ± 0.078
5.053GlyLys: 5.053 ± 0.063
6.465GlyLeu: 6.465 ± 0.081
2.016GlyMet: 2.016 ± 0.041
2.769GlyAsn: 2.769 ± 0.048
1.697GlyPro: 1.697 ± 0.041
2.213GlyGln: 2.213 ± 0.045
2.635GlyArg: 2.635 ± 0.042
3.751GlySer: 3.751 ± 0.056
3.819GlyThr: 3.819 ± 0.055
4.861GlyVal: 4.861 ± 0.069
0.766GlyTrp: 0.766 ± 0.027
2.785GlyTyr: 2.785 ± 0.05
0.0GlyXaa: 0.0 ± 0.0
His
1.279HisAla: 1.279 ± 0.037
0.218HisCys: 0.218 ± 0.014
1.019HisAsp: 1.019 ± 0.03
1.511HisGlu: 1.511 ± 0.037
1.175HisPhe: 1.175 ± 0.03
1.332HisGly: 1.332 ± 0.031
0.674HisHis: 0.674 ± 0.026
1.576HisIle: 1.576 ± 0.037
1.046HisLys: 1.046 ± 0.03
2.235HisLeu: 2.235 ± 0.041
0.476HisMet: 0.476 ± 0.018
0.784HisAsn: 0.784 ± 0.025
1.035HisPro: 1.035 ± 0.024
0.844HisGln: 0.844 ± 0.027
0.858HisArg: 0.858 ± 0.025
1.242HisSer: 1.242 ± 0.033
0.974HisThr: 0.974 ± 0.03
1.339HisVal: 1.339 ± 0.033
0.223HisTrp: 0.223 ± 0.017
0.975HisTyr: 0.975 ± 0.028
0.0HisXaa: 0.0 ± 0.0
Ile
6.316IleAla: 6.316 ± 0.072
0.687IleCys: 0.687 ± 0.027
4.265IleAsp: 4.265 ± 0.058
6.433IleGlu: 6.433 ± 0.084
3.627IlePhe: 3.627 ± 0.068
6.019IleGly: 6.019 ± 0.082
1.743IleHis: 1.743 ± 0.037
6.337IleIle: 6.337 ± 0.092
4.904IleLys: 4.904 ± 0.068
7.737IleLeu: 7.737 ± 0.1
1.875IleMet: 1.875 ± 0.04
3.514IleAsn: 3.514 ± 0.055
3.475IlePro: 3.475 ± 0.057
2.83IleGln: 2.83 ± 0.049
3.293IleArg: 3.293 ± 0.053
5.311IleSer: 5.311 ± 0.072
4.404IleThr: 4.404 ± 0.067
5.786IleVal: 5.786 ± 0.075
0.685IleTrp: 0.685 ± 0.027
2.609IleTyr: 2.609 ± 0.044
0.0IleXaa: 0.0 ± 0.0
Lys
4.712LysAla: 4.712 ± 0.062
0.378LysCys: 0.378 ± 0.017
3.585LysAsp: 3.585 ± 0.057
7.354LysGlu: 7.354 ± 0.091
2.06LysPhe: 2.06 ± 0.043
4.624LysGly: 4.624 ± 0.063
1.273LysHis: 1.273 ± 0.033
5.222LysIle: 5.222 ± 0.064
6.041LysLys: 6.041 ± 0.072
6.317LysLeu: 6.317 ± 0.076
2.268LysMet: 2.268 ± 0.045
3.487LysAsn: 3.487 ± 0.054
2.111LysPro: 2.111 ± 0.041
2.796LysGln: 2.796 ± 0.053
3.412LysArg: 3.412 ± 0.058
3.347LysSer: 3.347 ± 0.058
3.535LysThr: 3.535 ± 0.052
4.527LysVal: 4.527 ± 0.059
0.855LysTrp: 0.855 ± 0.031
2.25LysTyr: 2.25 ± 0.049
0.0LysXaa: 0.0 ± 0.0
Leu
7.413LeuAla: 7.413 ± 0.091
0.719LeuCys: 0.719 ± 0.025
4.603LeuAsp: 4.603 ± 0.059
7.157LeuGlu: 7.157 ± 0.075
5.005LeuPhe: 5.005 ± 0.078
6.374LeuGly: 6.374 ± 0.089
1.997LeuHis: 1.997 ± 0.036
7.724LeuIle: 7.724 ± 0.083
6.967LeuLys: 6.967 ± 0.082
10.754LeuLeu: 10.754 ± 0.13
2.578LeuMet: 2.578 ± 0.053
4.49LeuAsn: 4.49 ± 0.059
3.803LeuPro: 3.803 ± 0.054
3.899LeuGln: 3.899 ± 0.059
3.697LeuArg: 3.697 ± 0.057
6.679LeuSer: 6.679 ± 0.081
5.798LeuThr: 5.798 ± 0.065
6.315LeuVal: 6.315 ± 0.089
0.803LeuTrp: 0.803 ± 0.026
3.25LeuTyr: 3.25 ± 0.054
0.0LeuXaa: 0.0 ± 0.0
Met
1.939MetAla: 1.939 ± 0.034
0.152MetCys: 0.152 ± 0.009
1.33MetAsp: 1.33 ± 0.034
2.142MetGlu: 2.142 ± 0.043
1.088MetPhe: 1.088 ± 0.028
1.721MetGly: 1.721 ± 0.037
0.388MetHis: 0.388 ± 0.018
2.325MetIle: 2.325 ± 0.042
2.561MetLys: 2.561 ± 0.047
2.549MetLeu: 2.549 ± 0.049
0.828MetMet: 0.828 ± 0.024
1.605MetAsn: 1.605 ± 0.035
0.93MetPro: 0.93 ± 0.031
0.821MetGln: 0.821 ± 0.023
1.109MetArg: 1.109 ± 0.029
1.659MetSer: 1.659 ± 0.032
1.612MetThr: 1.612 ± 0.031
1.703MetVal: 1.703 ± 0.039
0.181MetTrp: 0.181 ± 0.012
0.739MetTyr: 0.739 ± 0.024
0.0MetXaa: 0.0 ± 0.0
Asn
2.75AsnAla: 2.75 ± 0.052
0.333AsnCys: 0.333 ± 0.015
2.206AsnAsp: 2.206 ± 0.041
3.803AsnGlu: 3.803 ± 0.057
1.688AsnPhe: 1.688 ± 0.041
3.433AsnGly: 3.433 ± 0.048
1.088AsnHis: 1.088 ± 0.027
3.62AsnIle: 3.62 ± 0.057
2.924AsnLys: 2.924 ± 0.047
3.944AsnLeu: 3.944 ± 0.051
1.238AsnMet: 1.238 ± 0.032
1.886AsnAsn: 1.886 ± 0.044
2.106AsnPro: 2.106 ± 0.052
1.804AsnGln: 1.804 ± 0.041
2.12AsnArg: 2.12 ± 0.039
2.421AsnSer: 2.421 ± 0.044
2.023AsnThr: 2.023 ± 0.041
3.017AsnVal: 3.017 ± 0.051
0.549AsnTrp: 0.549 ± 0.025
1.569AsnTyr: 1.569 ± 0.037
0.0AsnXaa: 0.0 ± 0.0
Pro
2.18ProAla: 2.18 ± 0.045
0.206ProCys: 0.206 ± 0.013
1.806ProAsp: 1.806 ± 0.044
2.762ProGlu: 2.762 ± 0.053
2.09ProPhe: 2.09 ± 0.046
2.124ProGly: 2.124 ± 0.04
0.842ProHis: 0.842 ± 0.028
2.967ProIle: 2.967 ± 0.054
2.143ProLys: 2.143 ± 0.042
3.599ProLeu: 3.599 ± 0.06
0.754ProMet: 0.754 ± 0.026
1.7ProAsn: 1.7 ± 0.043
0.965ProPro: 0.965 ± 0.034
1.145ProGln: 1.145 ± 0.031
1.107ProArg: 1.107 ± 0.029
2.243ProSer: 2.243 ± 0.043
1.964ProThr: 1.964 ± 0.038
2.526ProVal: 2.526 ± 0.048
0.352ProTrp: 0.352 ± 0.018
1.387ProTyr: 1.387 ± 0.042
0.0ProXaa: 0.0 ± 0.0
Gln
2.591GlnAla: 2.591 ± 0.048
0.237GlnCys: 0.237 ± 0.013
1.471GlnAsp: 1.471 ± 0.036
2.966GlnGlu: 2.966 ± 0.05
1.814GlnPhe: 1.814 ± 0.042
2.113GlnGly: 2.113 ± 0.04
0.779GlnHis: 0.779 ± 0.025
2.804GlnIle: 2.804 ± 0.058
2.856GlnLys: 2.856 ± 0.052
4.142GlnLeu: 4.142 ± 0.067
1.143GlnMet: 1.143 ± 0.032
1.618GlnAsn: 1.618 ± 0.035
1.072GlnPro: 1.072 ± 0.034
1.669GlnGln: 1.669 ± 0.053
1.518GlnArg: 1.518 ± 0.035
2.097GlnSer: 2.097 ± 0.045
1.888GlnThr: 1.888 ± 0.037
2.28GlnVal: 2.28 ± 0.048
0.381GlnTrp: 0.381 ± 0.017
1.37GlnTyr: 1.37 ± 0.031
0.0GlnXaa: 0.0 ± 0.0
Arg
2.663ArgAla: 2.663 ± 0.052
0.28ArgCys: 0.28 ± 0.015
1.915ArgAsp: 1.915 ± 0.041
3.314ArgGlu: 3.314 ± 0.051
1.943ArgPhe: 1.943 ± 0.034
2.32ArgGly: 2.32 ± 0.052
0.762ArgHis: 0.762 ± 0.025
3.175ArgIle: 3.175 ± 0.048
3.339ArgLys: 3.339 ± 0.055
4.121ArgLeu: 4.121 ± 0.061
1.26ArgMet: 1.26 ± 0.034
2.02ArgAsn: 2.02 ± 0.045
1.246ArgPro: 1.246 ± 0.038
1.489ArgGln: 1.489 ± 0.038
1.919ArgArg: 1.919 ± 0.042
2.178ArgSer: 2.178 ± 0.045
2.067ArgThr: 2.067 ± 0.041
2.641ArgVal: 2.641 ± 0.055
0.41ArgTrp: 0.41 ± 0.022
1.584ArgTyr: 1.584 ± 0.037
0.0ArgXaa: 0.0 ± 0.0
Ser
3.655SerAla: 3.655 ± 0.056
0.457SerCys: 0.457 ± 0.022
2.592SerAsp: 2.592 ± 0.043
4.101SerGlu: 4.101 ± 0.058
3.381SerPhe: 3.381 ± 0.055
4.343SerGly: 4.343 ± 0.061
1.238SerHis: 1.238 ± 0.031
5.154SerIle: 5.154 ± 0.067
3.752SerLys: 3.752 ± 0.058
6.156SerLeu: 6.156 ± 0.079
1.659SerMet: 1.659 ± 0.037
2.532SerAsn: 2.532 ± 0.049
2.072SerPro: 2.072 ± 0.04
1.963SerGln: 1.963 ± 0.04
2.191SerArg: 2.191 ± 0.038
3.851SerSer: 3.851 ± 0.058
3.044SerThr: 3.044 ± 0.054
3.935SerVal: 3.935 ± 0.059
0.592SerTrp: 0.592 ± 0.022
2.213SerTyr: 2.213 ± 0.039
0.0SerXaa: 0.0 ± 0.0
Thr
3.825ThrAla: 3.825 ± 0.062
0.35ThrCys: 0.35 ± 0.017
2.456ThrAsp: 2.456 ± 0.042
3.62ThrGlu: 3.62 ± 0.055
2.625ThrPhe: 2.625 ± 0.05
4.043ThrGly: 4.043 ± 0.057
0.978ThrHis: 0.978 ± 0.029
4.615ThrIle: 4.615 ± 0.066
3.359ThrLys: 3.359 ± 0.046
5.135ThrLeu: 5.135 ± 0.07
1.285ThrMet: 1.285 ± 0.036
2.389ThrAsn: 2.389 ± 0.05
2.087ThrPro: 2.087 ± 0.041
1.334ThrGln: 1.334 ± 0.037
1.885ThrArg: 1.885 ± 0.039
3.125ThrSer: 3.125 ± 0.045
2.901ThrThr: 2.901 ± 0.05
4.137ThrVal: 4.137 ± 0.053
0.472ThrTrp: 0.472 ± 0.022
1.862ThrTyr: 1.862 ± 0.035
0.0ThrXaa: 0.0 ± 0.0
Val
5.06ValAla: 5.06 ± 0.07
0.568ValCys: 0.568 ± 0.02
3.48ValAsp: 3.48 ± 0.053
5.141ValGlu: 5.141 ± 0.061
3.093ValPhe: 3.093 ± 0.056
4.562ValGly: 4.562 ± 0.068
1.358ValHis: 1.358 ± 0.033
5.674ValIle: 5.674 ± 0.077
4.699ValLys: 4.699 ± 0.057
6.67ValLeu: 6.67 ± 0.084
1.862ValMet: 1.862 ± 0.046
3.009ValAsn: 3.009 ± 0.052
2.513ValPro: 2.513 ± 0.042
2.352ValGln: 2.352 ± 0.042
2.567ValArg: 2.567 ± 0.05
4.396ValSer: 4.396 ± 0.06
3.891ValThr: 3.891 ± 0.056
4.947ValVal: 4.947 ± 0.062
0.562ValTrp: 0.562 ± 0.023
2.115ValTyr: 2.115 ± 0.044
0.0ValXaa: 0.0 ± 0.0
Trp
0.535TrpAla: 0.535 ± 0.021
0.076TrpCys: 0.076 ± 0.007
0.494TrpAsp: 0.494 ± 0.022
0.698TrpGlu: 0.698 ± 0.024
0.539TrpPhe: 0.539 ± 0.024
0.691TrpGly: 0.691 ± 0.023
0.224TrpHis: 0.224 ± 0.014
0.806TrpIle: 0.806 ± 0.028
0.687TrpLys: 0.687 ± 0.022
1.148TrpLeu: 1.148 ± 0.03
0.299TrpMet: 0.299 ± 0.015
0.505TrpAsn: 0.505 ± 0.021
0.233TrpPro: 0.233 ± 0.014
0.388TrpGln: 0.388 ± 0.02
0.385TrpArg: 0.385 ± 0.017
0.58TrpSer: 0.58 ± 0.022
0.515TrpThr: 0.515 ± 0.024
0.592TrpVal: 0.592 ± 0.024
0.145TrpTrp: 0.145 ± 0.012
0.351TrpTyr: 0.351 ± 0.019
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.036TyrAla: 2.036 ± 0.042
0.321TyrCys: 0.321 ± 0.015
1.869TyrAsp: 1.869 ± 0.035
2.742TyrGlu: 2.742 ± 0.046
1.859TyrPhe: 1.859 ± 0.041
2.468TyrGly: 2.468 ± 0.044
0.899TyrHis: 0.899 ± 0.026
2.614TyrIle: 2.614 ± 0.045
2.048TyrLys: 2.048 ± 0.043
3.557TyrLeu: 3.557 ± 0.054
0.845TyrMet: 0.845 ± 0.026
1.402TyrAsn: 1.402 ± 0.037
1.408TyrPro: 1.408 ± 0.036
1.541TyrGln: 1.541 ± 0.035
1.637TyrArg: 1.637 ± 0.035
2.132TyrSer: 2.132 ± 0.042
1.714TyrThr: 1.714 ± 0.039
2.168TyrVal: 2.168 ± 0.036
0.381TyrTrp: 0.381 ± 0.018
1.422TyrTyr: 1.422 ± 0.036
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4463 proteins (1284409 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski