Devosia epidermidihirudinis
Average proteome isoelectric point is 6.21
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3353 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0F5Q3M8|A0A0F5Q3M8_9RHIZ Alanine--tRNA ligase OS=Devosia epidermidihirudinis OX=1293439 GN=alaS PE=3 SV=1
MM1 pKa = 7.76 AISPTDD7 pKa = 2.71 WGTRR11 pKa = 11.84 YY12 pKa = 10.46 APTLDD17 pKa = 5.03 DD18 pKa = 4.03 IEE20 pKa = 5.22 GLASQALKK28 pKa = 10.64 DD29 pKa = 3.7 LPEE32 pKa = 4.41 PFRR35 pKa = 11.84 SLAADD40 pKa = 3.86 VTCSVADD47 pKa = 3.7 YY48 pKa = 11.22 AEE50 pKa = 4.09 EE51 pKa = 5.82 DD52 pKa = 3.37 ILASFGMEE60 pKa = 4.23 SPFEE64 pKa = 3.98 LMGLFSGIGMTEE76 pKa = 4.33 DD77 pKa = 3.09 GAMPQTGQLPNTVYY91 pKa = 10.78 LYY93 pKa = 10.82 RR94 pKa = 11.84 RR95 pKa = 11.84 AILDD99 pKa = 3.21 YY100 pKa = 10.2 WAEE103 pKa = 4.11 HH104 pKa = 7.43 DD105 pKa = 5.11 DD106 pKa = 3.79 NTLGEE111 pKa = 4.62 VVTHH115 pKa = 5.82 VLIHH119 pKa = 5.98 EE120 pKa = 5.08 LGHH123 pKa = 6.39 HH124 pKa = 6.58 FGFSDD129 pKa = 3.6 EE130 pKa = 4.25 DD131 pKa = 3.62 MEE133 pKa = 6.46 AIEE136 pKa = 4.75 SSADD140 pKa = 3.36 EE141 pKa = 4.32 SS142 pKa = 3.89
Molecular weight: 15.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.758
IPC2_protein 3.897
IPC_protein 3.859
Toseland 3.668
ProMoST 4.012
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.757
Rodwell 3.694
Grimsley 3.579
Solomon 3.834
Lehninger 3.783
Nozaki 3.961
DTASelect 4.151
Thurlkill 3.706
EMBOSS 3.77
Sillero 3.986
Patrickios 1.888
IPC_peptide 3.834
IPC2_peptide 3.961
IPC2.peptide.svr19 3.876
Protein with the highest isoelectric point:
>tr|A0A0F5QBT7|A0A0F5QBT7_9RHIZ Multidrug ABC transporter OS=Devosia epidermidihirudinis OX=1293439 GN=WH87_11225 PE=3 SV=1
MM1 pKa = 7.21 SVWIGAVMVGAVFFTSTLSGIFGMAGGLILLAILLSIMPVGTAIAVQGAIQIISNGSRR59 pKa = 11.84 AFLSRR64 pKa = 11.84 AFIDD68 pKa = 3.26 WRR70 pKa = 11.84 VLGTICLGLLTAGILLFILRR90 pKa = 11.84 YY91 pKa = 7.24 TPSLATVCIVIGLMPILVWIPRR113 pKa = 11.84 DD114 pKa = 3.42 WLALDD119 pKa = 3.66 ASKK122 pKa = 10.37 PLHH125 pKa = 6.55 AFICGFVGGGLNLAVGVSGPTIDD148 pKa = 3.41 IFFIRR153 pKa = 11.84 TPMDD157 pKa = 2.86 RR158 pKa = 11.84 RR159 pKa = 11.84 KK160 pKa = 9.85 IVATKK165 pKa = 10.38 AATQVISHH173 pKa = 6.24 GAKK176 pKa = 9.94 VVFYY180 pKa = 9.86 WNATMVLTPGEE191 pKa = 4.24 WIAVLIAAPFAIGGANVGFWVLQRR215 pKa = 11.84 LSDD218 pKa = 3.89 ANFKK222 pKa = 10.44 RR223 pKa = 11.84 WTRR226 pKa = 11.84 WIVTAVGIFYY236 pKa = 9.58 MSRR239 pKa = 11.84 GISLAFGAA247 pKa = 5.05
Molecular weight: 26.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.349
IPC2_protein 9.633
IPC_protein 10.423
Toseland 10.584
ProMoST 10.277
Dawson 10.701
Bjellqvist 10.423
Wikipedia 10.906
Rodwell 10.891
Grimsley 10.745
Solomon 10.804
Lehninger 10.774
Nozaki 10.599
DTASelect 10.409
Thurlkill 10.584
EMBOSS 10.979
Sillero 10.628
Patrickios 10.657
IPC_peptide 10.804
IPC2_peptide 9.619
IPC2.peptide.svr19 8.513
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3353
0
3353
1031883
41
2833
307.7
33.3
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.187 ± 0.055
0.64 ± 0.01
5.804 ± 0.036
5.406 ± 0.041
3.859 ± 0.027
8.537 ± 0.042
1.957 ± 0.019
5.694 ± 0.03
3.352 ± 0.032
10.301 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.512 ± 0.02
2.925 ± 0.029
4.891 ± 0.032
3.209 ± 0.023
6.151 ± 0.043
5.571 ± 0.029
5.652 ± 0.035
7.722 ± 0.035
1.319 ± 0.018
2.314 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here