Fonsecaea multimorphosa CBS 102226
Average proteome isoelectric point is 6.41
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 12368 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0D2I3S9|A0A0D2I3S9_9EURO Uncharacterized protein OS=Fonsecaea multimorphosa CBS 102226 OX=1442371 GN=Z520_12340 PE=4 SV=1
MM1 pKa = 7.4 RR2 pKa = 11.84 HH3 pKa = 5.85 LAAFCLLFPLLLLTEE18 pKa = 4.88 LVTGAPSSSLQEE30 pKa = 3.87 YY31 pKa = 9.77 NRR33 pKa = 11.84 RR34 pKa = 11.84 SSKK37 pKa = 10.86 SILLKK42 pKa = 9.18 TRR44 pKa = 11.84 RR45 pKa = 11.84 VNKK48 pKa = 6.91 PTLRR52 pKa = 11.84 RR53 pKa = 11.84 RR54 pKa = 11.84 QDD56 pKa = 3.58 DD57 pKa = 3.53 GDD59 pKa = 4.12 SFLTTIAWDD68 pKa = 3.71 PTEE71 pKa = 4.05 SQYY74 pKa = 11.19 EE75 pKa = 3.93 ISVGIGTPLQDD86 pKa = 3.03 ILLVVDD92 pKa = 4.62 TGSSDD97 pKa = 2.89 TWAFPPDD104 pKa = 3.71 LCSITPNCFWSFDD117 pKa = 3.95 PDD119 pKa = 3.19 QSSTYY124 pKa = 9.9 EE125 pKa = 4.1 VVGSGAFSITYY136 pKa = 10.26 EE137 pKa = 4.15 EE138 pKa = 4.73 GTSVASGDD146 pKa = 3.81 FFKK149 pKa = 10.64 DD150 pKa = 3.03 TASFEE155 pKa = 4.39 GGVQVEE161 pKa = 4.37 DD162 pKa = 3.7 VQFGLATAITGNLSYY177 pKa = 10.93 GVAGLGFDD185 pKa = 3.84 TNEE188 pKa = 4.46 AEE190 pKa = 4.36 PGYY193 pKa = 9.89 PDD195 pKa = 5.86 LIDD198 pKa = 3.7 MLVSSGTIDD207 pKa = 3.35 TPLFSLWSDD216 pKa = 4.34 LIGPDD221 pKa = 2.89 SGGYY225 pKa = 9.57 ILFGGVDD232 pKa = 3.37 EE233 pKa = 4.63 TAYY236 pKa = 10.17 KK237 pKa = 10.71 GDD239 pKa = 3.75 IVWRR243 pKa = 11.84 PMVADD248 pKa = 4.05 DD249 pKa = 4.2 DD250 pKa = 4.48 GVYY253 pKa = 9.99 RR254 pKa = 11.84 EE255 pKa = 4.11 YY256 pKa = 11.06 AITLTGWGTTADD268 pKa = 3.91 PPKK271 pKa = 10.44 QLVPVDD277 pKa = 3.92 VKK279 pKa = 11.47 LDD281 pKa = 3.53 MLLDD285 pKa = 3.96 SGTTNIVVPQDD296 pKa = 3.37 MYY298 pKa = 11.53 NAIVGYY304 pKa = 10.54 LPFAVNNGNVDD315 pKa = 3.65 CTGIPAGSVDD325 pKa = 4.58 FYY327 pKa = 11.44 FGDD330 pKa = 3.73 EE331 pKa = 4.37 LKK333 pKa = 10.69 ISIPFQALVLPLSPGSDD350 pKa = 2.99 SCFFGIEE357 pKa = 4.15 VASEE361 pKa = 4.21 GGWLILGDD369 pKa = 3.77 TFLQNAYY376 pKa = 10.26 VAFDD380 pKa = 3.74 LANYY384 pKa = 9.82 RR385 pKa = 11.84 FGIAPTNYY393 pKa = 9.83 GPTDD397 pKa = 3.35 SSS399 pKa = 3.57
Molecular weight: 43.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.681
IPC2_protein 3.783
IPC_protein 3.821
Toseland 3.579
ProMoST 3.986
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.757
Rodwell 3.63
Grimsley 3.49
Solomon 3.808
Lehninger 3.77
Nozaki 3.923
DTASelect 4.202
Thurlkill 3.643
EMBOSS 3.77
Sillero 3.935
Patrickios 1.291
IPC_peptide 3.808
IPC2_peptide 3.91
IPC2.peptide.svr19 3.827
Protein with the highest isoelectric point:
>tr|A0A0D2JTP6|A0A0D2JTP6_9EURO Peptidase A1 domain-containing protein OS=Fonsecaea multimorphosa CBS 102226 OX=1442371 GN=Z520_07567 PE=3 SV=1
MM1 pKa = 7.78 PNLFGLLRR9 pKa = 11.84 LGCDD13 pKa = 3.34 GADD16 pKa = 3.29 RR17 pKa = 11.84 RR18 pKa = 11.84 PGQHH22 pKa = 6.19 LNSYY26 pKa = 7.43 TVQSLLKK33 pKa = 9.33 TYY35 pKa = 11.1 LNLTISSRR43 pKa = 11.84 LCEE46 pKa = 4.03 GLPLVGHH53 pKa = 7.27 DD54 pKa = 4.95 AMLIPQEE61 pKa = 4.52 SGSHH65 pKa = 6.34 SSTQVPSNTVLRR77 pKa = 11.84 FRR79 pKa = 11.84 IGAYY83 pKa = 7.98 SRR85 pKa = 11.84 KK86 pKa = 8.69 PRR88 pKa = 11.84 VVSHH92 pKa = 6.99 RR93 pKa = 11.84 GPVRR97 pKa = 11.84 AQLNLPQHH105 pKa = 5.59 VRR107 pKa = 11.84 PHH109 pKa = 6.37 INAHH113 pKa = 5.53 HH114 pKa = 7.05 PNRR117 pKa = 11.84 NLIHH121 pKa = 6.36 NNSRR125 pKa = 11.84 KK126 pKa = 8.2 PTQDD130 pKa = 2.72 QQNEE134 pKa = 4.35 HH135 pKa = 6.26 NPVTFGEE142 pKa = 4.13 PKK144 pKa = 9.94 ILYY147 pKa = 8.74 EE148 pKa = 4.31 PFWIFPTKK156 pKa = 10.83 DD157 pKa = 3.17 NANTRR162 pKa = 11.84 SGVQFCKK169 pKa = 10.21 SDD171 pKa = 3.59 SVRR174 pKa = 11.84 SRR176 pKa = 11.84 ATRR179 pKa = 3.26
Molecular weight: 20.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.353
IPC2_protein 9.663
IPC_protein 10.57
Toseland 10.643
ProMoST 10.628
Dawson 10.745
Bjellqvist 10.496
Wikipedia 10.979
Rodwell 10.891
Grimsley 10.804
Solomon 10.877
Lehninger 10.847
Nozaki 10.643
DTASelect 10.482
Thurlkill 10.643
EMBOSS 11.052
Sillero 10.687
Patrickios 10.613
IPC_peptide 10.877
IPC2_peptide 9.677
IPC2.peptide.svr19 8.639
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
12368
0
12368
6118718
39
6136
494.7
54.75
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.711 ± 0.018
1.229 ± 0.007
5.671 ± 0.016
6.121 ± 0.021
3.745 ± 0.012
6.888 ± 0.021
2.451 ± 0.008
4.852 ± 0.014
4.707 ± 0.019
8.991 ± 0.018
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.17 ± 0.008
3.561 ± 0.011
6.064 ± 0.018
4.14 ± 0.015
6.129 ± 0.02
8.133 ± 0.026
5.986 ± 0.017
6.19 ± 0.015
1.491 ± 0.007
2.77 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here