Xanthophyllomyces dendrorhous virus L1A
Average proteome isoelectric point is 6.67
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|H9XW60|H9XW60_9VIRU RNA-directed RNA polymerase OS=Xanthophyllomyces dendrorhous virus L1A OX=1167690 GN=RdRp PE=3 SV=1
MM1 pKa = 6.6 HH2 pKa = 7.25 TKK4 pKa = 10.42 FIEE7 pKa = 4.68 SIVKK11 pKa = 9.02 TDD13 pKa = 3.49 YY14 pKa = 11.07 EE15 pKa = 4.97 GSFQPLFKK23 pKa = 10.6 DD24 pKa = 4.44 GRR26 pKa = 11.84 FTLINKK32 pKa = 5.42 QTAMVDD38 pKa = 3.28 MLGRR42 pKa = 11.84 QYY44 pKa = 11.6 DD45 pKa = 4.03 VNSEE49 pKa = 3.92 LASNFNVCGKK59 pKa = 10.26 KK60 pKa = 10.23 PEE62 pKa = 4.03 VLHH65 pKa = 6.37 NVGEE69 pKa = 4.28 ADD71 pKa = 3.91 FSGLNRR77 pKa = 11.84 KK78 pKa = 9.13 YY79 pKa = 10.82 FDD81 pKa = 3.85 DD82 pKa = 3.97 TNTYY86 pKa = 10.69 SPLAAINEE94 pKa = 4.2 FTKK97 pKa = 10.39 FVPGVRR103 pKa = 11.84 LTKK106 pKa = 10.93 GEE108 pKa = 3.61 VDD110 pKa = 4.29 AFAKK114 pKa = 9.64 SQIYY118 pKa = 10.15 EE119 pKa = 4.26 DD120 pKa = 3.74 SQEE123 pKa = 4.29 AYY125 pKa = 9.08 ILNMLISWFKK135 pKa = 10.75 ALLYY139 pKa = 10.68 EE140 pKa = 4.57 DD141 pKa = 4.79 TNSKK145 pKa = 11.34 DD146 pKa = 3.37 NVLHH150 pKa = 5.94 VKK152 pKa = 10.26 QSGYY156 pKa = 10.29 QDD158 pKa = 3.14 SHH160 pKa = 7.41 VKK162 pKa = 10.51 AEE164 pKa = 4.15 YY165 pKa = 10.75 GGVTGDD171 pKa = 3.52 VVHH174 pKa = 7.45 EE175 pKa = 4.36 INMGPPVDD183 pKa = 5.01 DD184 pKa = 3.92 MPEE187 pKa = 3.68 VMMMVRR193 pKa = 11.84 SKK195 pKa = 11.39 DD196 pKa = 3.75 NYY198 pKa = 7.82 WDD200 pKa = 3.42 RR201 pKa = 11.84 PYY203 pKa = 11.51 VLRR206 pKa = 11.84 FDD208 pKa = 4.24 NRR210 pKa = 11.84 SSAQYY215 pKa = 9.53 TFYY218 pKa = 9.79 ITHH221 pKa = 6.56 CFGRR225 pKa = 11.84 DD226 pKa = 3.06 GTSPLNVDD234 pKa = 2.68 IHH236 pKa = 6.98 IPSVDD241 pKa = 3.88 FDD243 pKa = 3.74 QMLFEE248 pKa = 4.74 PASGAMRR255 pKa = 11.84 SITDD259 pKa = 3.67 PAALPWCKK267 pKa = 10.43 SGTLFGWIKK276 pKa = 10.96 DD277 pKa = 3.75 YY278 pKa = 10.72 VTLNRR283 pKa = 11.84 VEE285 pKa = 4.42 RR286 pKa = 11.84 AFSAAFEE293 pKa = 4.3 TLTAIAFTPMPSYY306 pKa = 10.77 QEE308 pKa = 3.86 SLVWDD313 pKa = 3.76 KK314 pKa = 11.55 AITQVVIAKK323 pKa = 7.73 FAPCRR328 pKa = 11.84 AKK330 pKa = 10.42 IPSNLEE336 pKa = 3.47 GEE338 pKa = 4.6 AMVTDD343 pKa = 4.32 MDD345 pKa = 3.62 AHH347 pKa = 6.86 DD348 pKa = 4.25 FVLDD352 pKa = 3.57 EE353 pKa = 4.47 TKK355 pKa = 10.27 TPRR358 pKa = 11.84 KK359 pKa = 9.81 ALFSGAMINYY369 pKa = 8.28 LAFMGLHH376 pKa = 7.25 AILSNYY382 pKa = 9.79 ASRR385 pKa = 11.84 HH386 pKa = 4.73 EE387 pKa = 4.13 NWRR390 pKa = 11.84 SAFLHH395 pKa = 5.22 SHH397 pKa = 6.33 EE398 pKa = 4.77 EE399 pKa = 3.74 LAILHH404 pKa = 6.54 DD405 pKa = 3.81 RR406 pKa = 11.84 TVRR409 pKa = 11.84 AALTSVITGKK419 pKa = 10.85 EE420 pKa = 3.78 MVTFMNPNLFVSYY433 pKa = 10.77 DD434 pKa = 3.55 VTPMVNVSKK443 pKa = 11.19 VSFEE447 pKa = 4.09 EE448 pKa = 4.1 VLEE451 pKa = 4.16 RR452 pKa = 11.84 GYY454 pKa = 10.17 PSSLPVHH461 pKa = 6.27 GVVPMVSGSLFLSANASDD479 pKa = 4.37 VASQCHH485 pKa = 6.85 LEE487 pKa = 4.07 QGMKK491 pKa = 9.04 VTVDD495 pKa = 3.26 EE496 pKa = 4.75 YY497 pKa = 11.04 GTVSPEE503 pKa = 3.53 DD504 pKa = 3.85 ALRR507 pKa = 11.84 VAQMYY512 pKa = 10.88 RR513 pKa = 11.84 MFGHH517 pKa = 6.19 EE518 pKa = 4.12 LEE520 pKa = 4.38 IRR522 pKa = 11.84 SEE524 pKa = 3.98 KK525 pKa = 10.01 TEE527 pKa = 4.36 EE528 pKa = 3.69 IHH530 pKa = 7.29 EE531 pKa = 4.28 LFAPVQEE538 pKa = 4.3 CVIYY542 pKa = 9.85 PSALLYY548 pKa = 8.17 NTRR551 pKa = 11.84 DD552 pKa = 3.34 TDD554 pKa = 3.73 RR555 pKa = 11.84 LKK557 pKa = 11.0 LVSSLRR563 pKa = 11.84 RR564 pKa = 11.84 PGRR567 pKa = 11.84 SSTIPDD573 pKa = 3.48 VSALTAGQTITIDD586 pKa = 3.38 YY587 pKa = 7.12 TIPRR591 pKa = 11.84 IGMHH595 pKa = 5.85 YY596 pKa = 9.88 FNKK599 pKa = 9.87 RR600 pKa = 11.84 VLPIAPSMVLPQRR613 pKa = 11.84 KK614 pKa = 8.71 RR615 pKa = 11.84 EE616 pKa = 3.52 ITFRR620 pKa = 11.84 VKK622 pKa = 10.49 GHH624 pKa = 5.42 TVLHH628 pKa = 5.54 KK629 pKa = 11.2 VKK631 pKa = 9.95 MNASNVTRR639 pKa = 11.84 PVKK642 pKa = 10.26 DD643 pKa = 3.31 FHH645 pKa = 8.44 GGEE648 pKa = 4.05 IQVAPLLPVLRR659 pKa = 11.84 NARR662 pKa = 11.84 VPIQVNQTSEE672 pKa = 4.1 EE673 pKa = 4.33 DD674 pKa = 3.45 VTAEE678 pKa = 4.53 DD679 pKa = 4.17 VEE681 pKa = 4.46
Molecular weight: 76.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.555
IPC2_protein 5.474
IPC_protein 5.512
Toseland 5.893
ProMoST 5.766
Dawson 5.715
Bjellqvist 5.753
Wikipedia 5.703
Rodwell 5.69
Grimsley 6.059
Solomon 5.715
Lehninger 5.703
Nozaki 5.957
DTASelect 6.148
Thurlkill 6.109
EMBOSS 6.071
Sillero 6.059
Patrickios 4.113
IPC_peptide 5.728
IPC2_peptide 6.059
IPC2.peptide.svr19 6.012
Protein with the highest isoelectric point:
>tr|H9XW60|H9XW60_9VIRU RNA-directed RNA polymerase OS=Xanthophyllomyces dendrorhous virus L1A OX=1167690 GN=RdRp PE=3 SV=1
MM1 pKa = 7.83 DD2 pKa = 5.36 RR3 pKa = 11.84 EE4 pKa = 4.3 SSNKK8 pKa = 9.75 FGIFEE13 pKa = 4.27 ASKK16 pKa = 10.31 SVYY19 pKa = 9.35 GVTMSGLVIANGKK32 pKa = 10.1 AKK34 pKa = 10.47 FFFASIKK41 pKa = 10.42 QYY43 pKa = 11.63 LLTQSKK49 pKa = 10.96 AMLIAMTRR57 pKa = 11.84 HH58 pKa = 5.62 FSSLYY63 pKa = 10.08 EE64 pKa = 4.1 SYY66 pKa = 10.94 AYY68 pKa = 9.96 DD69 pKa = 4.92 DD70 pKa = 3.63 PTSPVNLFRR79 pKa = 11.84 DD80 pKa = 4.26 RR81 pKa = 11.84 FPGRR85 pKa = 11.84 SEE87 pKa = 3.9 NEE89 pKa = 3.44 AMTFKK94 pKa = 10.8 QMEE97 pKa = 4.63 EE98 pKa = 4.08 LPKK101 pKa = 10.82 SKK103 pKa = 10.27 ISGAHH108 pKa = 6.2 HH109 pKa = 6.14 FHH111 pKa = 6.6 FTPMQVLKK119 pKa = 10.88 SIGRR123 pKa = 11.84 GRR125 pKa = 11.84 LEE127 pKa = 3.85 RR128 pKa = 11.84 AIQASRR134 pKa = 11.84 LPKK137 pKa = 9.91 DD138 pKa = 3.42 AEE140 pKa = 4.25 MTFATGMLLWYY151 pKa = 9.35 TSISDD156 pKa = 3.87 QMAEE160 pKa = 4.66 LIRR163 pKa = 11.84 GAGIFKK169 pKa = 10.01 CDD171 pKa = 3.36 SVRR174 pKa = 11.84 EE175 pKa = 4.12 YY176 pKa = 10.96 VKK178 pKa = 10.57 EE179 pKa = 3.9 MKK181 pKa = 10.14 QLSVEE186 pKa = 4.21 AKK188 pKa = 9.82 SLQNLVTDD196 pKa = 4.2 DD197 pKa = 3.85 LRR199 pKa = 11.84 TVFEE203 pKa = 5.09 LEE205 pKa = 4.12 VLVNRR210 pKa = 11.84 IDD212 pKa = 4.99 GVVDD216 pKa = 3.21 WEE218 pKa = 4.29 KK219 pKa = 11.0 EE220 pKa = 3.93 KK221 pKa = 11.17 EE222 pKa = 3.93 NRR224 pKa = 11.84 QSVNVTNIKK233 pKa = 10.73 DD234 pKa = 3.44 SDD236 pKa = 4.06 VFRR239 pKa = 11.84 SACKK243 pKa = 9.98 IFEE246 pKa = 4.29 DD247 pKa = 3.59 AKK249 pKa = 11.17 GIGRR253 pKa = 11.84 RR254 pKa = 11.84 PKK256 pKa = 8.95 SFSWEE261 pKa = 4.31 SYY263 pKa = 7.49 WANRR267 pKa = 11.84 WQWSAAGSIHH277 pKa = 5.62 SQYY280 pKa = 9.7 PRR282 pKa = 11.84 DD283 pKa = 3.68 MEE285 pKa = 4.27 YY286 pKa = 10.8 VIRR289 pKa = 11.84 DD290 pKa = 3.57 QQSLKK295 pKa = 11.0 NKK297 pKa = 10.11 FITISNMPKK306 pKa = 9.02 CTVDD310 pKa = 3.33 YY311 pKa = 10.82 FSDD314 pKa = 4.49 RR315 pKa = 11.84 EE316 pKa = 4.11 PQVQGWSSTKK326 pKa = 10.34 YY327 pKa = 8.99 EE328 pKa = 3.89 WGKK331 pKa = 9.95 QRR333 pKa = 11.84 AIYY336 pKa = 8.53 GTDD339 pKa = 2.92 LTSYY343 pKa = 10.13 VLSNFAFYY351 pKa = 10.89 NCEE354 pKa = 3.76 NVLPNQFPVGRR365 pKa = 11.84 DD366 pKa = 3.35 ANDD369 pKa = 3.18 EE370 pKa = 4.19 NVVNRR375 pKa = 11.84 VSGVLNNRR383 pKa = 11.84 MPFCLDD389 pKa = 3.3 FEE391 pKa = 5.27 DD392 pKa = 5.67 FNSQHH397 pKa = 6.66 SSGNMKK403 pKa = 10.31 AVIYY407 pKa = 10.07 AYY409 pKa = 9.49 IEE411 pKa = 4.12 TFIDD415 pKa = 3.98 CLTPEE420 pKa = 4.15 QEE422 pKa = 4.28 QAAMWTAASLDD433 pKa = 3.61 KK434 pKa = 11.09 QIINDD439 pKa = 3.53 NVGTKK444 pKa = 7.34 TTYY447 pKa = 9.61 EE448 pKa = 4.16 SKK450 pKa = 9.96 GTLLSGWRR458 pKa = 11.84 LTTFMNSVLNYY469 pKa = 10.02 IYY471 pKa = 7.55 TTKK474 pKa = 10.69 LAAEE478 pKa = 4.62 EE479 pKa = 4.13 KK480 pKa = 10.42 RR481 pKa = 11.84 PGDD484 pKa = 3.75 SLHH487 pKa = 6.73 NGDD490 pKa = 5.07 DD491 pKa = 3.41 VLIGVRR497 pKa = 11.84 SMALPQRR504 pKa = 11.84 CMQNAIKK511 pKa = 10.83 YY512 pKa = 6.95 NVRR515 pKa = 11.84 MQSSKK520 pKa = 10.74 CAVGAIAEE528 pKa = 4.3 FLRR531 pKa = 11.84 IDD533 pKa = 3.95 HH534 pKa = 6.25 KK535 pKa = 11.27 QGGNGQYY542 pKa = 10.68 LSRR545 pKa = 11.84 AVATMVHH552 pKa = 5.78 SRR554 pKa = 11.84 IEE556 pKa = 4.29 SRR558 pKa = 11.84 VSTDD562 pKa = 2.8 IRR564 pKa = 11.84 DD565 pKa = 3.73 LVQSMEE571 pKa = 4.09 NRR573 pKa = 11.84 FADD576 pKa = 3.42 AKK578 pKa = 10.21 GRR580 pKa = 11.84 GMANDD585 pKa = 4.26 IISGLRR591 pKa = 11.84 EE592 pKa = 3.8 QYY594 pKa = 11.27 YY595 pKa = 8.9 MRR597 pKa = 11.84 QSVLCDD603 pKa = 3.13 TDD605 pKa = 4.19 VEE607 pKa = 4.65 DD608 pKa = 5.77 IYY610 pKa = 11.07 LIKK613 pKa = 10.46 NAHH616 pKa = 5.77 RR617 pKa = 11.84 VVGGISEE624 pKa = 4.29 EE625 pKa = 4.34 KK626 pKa = 9.6 DD627 pKa = 3.09 SKK629 pKa = 10.66 MGVLITSQLRR639 pKa = 11.84 AQKK642 pKa = 10.22 NVSIPYY648 pKa = 9.59 LPGVNEE654 pKa = 4.14 YY655 pKa = 11.33 ANEE658 pKa = 3.48 IHH660 pKa = 7.09 KK661 pKa = 10.42 ALKK664 pKa = 10.21 INVSIKK670 pKa = 8.99 TICDD674 pKa = 3.11 RR675 pKa = 11.84 LYY677 pKa = 10.49 DD678 pKa = 3.66 ATYY681 pKa = 9.7 EE682 pKa = 4.03 AVSIKK687 pKa = 9.9 DD688 pKa = 3.25 RR689 pKa = 11.84 KK690 pKa = 9.67 MKK692 pKa = 10.12 ILRR695 pKa = 11.84 EE696 pKa = 3.97 NRR698 pKa = 11.84 DD699 pKa = 3.03 QWFVNVRR706 pKa = 11.84 RR707 pKa = 11.84 IYY709 pKa = 9.94 KK710 pKa = 9.23 AHH712 pKa = 7.27 KK713 pKa = 10.14 GSQLSQNYY721 pKa = 8.66 GKK723 pKa = 10.57 AALVGFALEE732 pKa = 4.03 VLGRR736 pKa = 11.84 EE737 pKa = 4.3 MPDD740 pKa = 2.98 ATITTILNTSQRR752 pKa = 11.84 PLDD755 pKa = 4.63 LIKK758 pKa = 10.78 HH759 pKa = 5.69 ILL761 pKa = 3.48
Molecular weight: 86.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.789
IPC2_protein 8.039
IPC_protein 7.878
Toseland 7.98
ProMoST 8.434
Dawson 8.712
Bjellqvist 8.814
Wikipedia 8.741
Rodwell 8.785
Grimsley 8.039
Solomon 8.785
Lehninger 8.77
Nozaki 8.916
DTASelect 8.639
Thurlkill 8.726
EMBOSS 8.873
Sillero 8.96
Patrickios 4.482
IPC_peptide 8.77
IPC2_peptide 7.556
IPC2.peptide.svr19 7.763
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1442
681
761
721.0
81.67
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.074 ± 0.078
1.11 ± 0.147
5.895 ± 0.081
6.103 ± 0.136
4.508 ± 0.311
5.27 ± 0.178
2.358 ± 0.466
5.687 ± 0.54
5.756 ± 0.584
7.42 ± 0.138
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.675 ± 0.091
4.993 ± 0.377
3.883 ± 0.901
3.814 ± 0.563
5.825 ± 0.44
8.044 ± 0.357
5.617 ± 0.447
7.906 ± 0.864
1.179 ± 0.191
3.883 ± 0.136
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here