Arthrobacter crusticola

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Micrococcaceae; Arthrobacter

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3325 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R5TUM1|A0A4R5TUM1_9MICC Orotate phosphoribosyltransferase OS=Arthrobacter crusticola OX=2547960 GN=pyrE PE=3 SV=1
MM1 pKa = 7.29KK2 pKa = 10.27VLRR5 pKa = 11.84FGRR8 pKa = 11.84AAAVISVAALALTACGSDD26 pKa = 2.97NATNAPAGEE35 pKa = 4.36AGPAEE40 pKa = 4.29STAAAVSGTLTGAGSSAQNAAMTAWQSGFQSAQSGATVQYY80 pKa = 10.92SPDD83 pKa = 3.26GSGAGRR89 pKa = 11.84DD90 pKa = 3.31AFLAQGVQFAGSDD103 pKa = 3.21AALDD107 pKa = 3.89DD108 pKa = 4.4EE109 pKa = 5.11EE110 pKa = 5.87YY111 pKa = 10.4EE112 pKa = 4.2ASKK115 pKa = 10.04EE116 pKa = 3.78ICGPDD121 pKa = 3.4GAINIPAYY129 pKa = 10.17VSPIAVAFNLPGIEE143 pKa = 4.39SLNLDD148 pKa = 3.97ADD150 pKa = 4.74TIASIFRR157 pKa = 11.84GEE159 pKa = 3.83IEE161 pKa = 4.02TWDD164 pKa = 3.98DD165 pKa = 3.38EE166 pKa = 5.43AIASQNEE173 pKa = 4.49GVEE176 pKa = 4.25LPDD179 pKa = 3.36TAVTVVHH186 pKa = 6.9RR187 pKa = 11.84SDD189 pKa = 3.74EE190 pKa = 4.41SGTTEE195 pKa = 4.58NFTEE199 pKa = 4.44YY200 pKa = 10.44LAQAAPEE207 pKa = 4.14VWTDD211 pKa = 3.45EE212 pKa = 5.1ASGEE216 pKa = 4.14WPSAIVAEE224 pKa = 4.45NAQGTNGVVSTTSSTEE240 pKa = 3.78GAVTYY245 pKa = 10.18TDD247 pKa = 3.47ASAVGDD253 pKa = 4.18LGQVHH258 pKa = 5.68VKK260 pKa = 10.44VGEE263 pKa = 4.21EE264 pKa = 3.99YY265 pKa = 11.25VEE267 pKa = 4.37LSSEE271 pKa = 4.07AAALAVEE278 pKa = 4.51AATPVEE284 pKa = 4.12GRR286 pKa = 11.84SDD288 pKa = 3.08VDD290 pKa = 3.18MSLEE294 pKa = 4.14LEE296 pKa = 4.41RR297 pKa = 11.84DD298 pKa = 3.68TEE300 pKa = 4.13ASGAYY305 pKa = 8.79PVVLVSYY312 pKa = 10.15HH313 pKa = 6.86IYY315 pKa = 10.27CSTYY319 pKa = 10.2EE320 pKa = 4.06DD321 pKa = 4.15QEE323 pKa = 4.54TVDD326 pKa = 4.02LVKK329 pKa = 11.0AFASYY334 pKa = 10.7AVSEE338 pKa = 4.34QGQQDD343 pKa = 3.39ASEE346 pKa = 4.44SAGNAPISEE355 pKa = 4.42TLRR358 pKa = 11.84EE359 pKa = 4.01QALTAIDD366 pKa = 4.77SITVASS372 pKa = 3.93

Molecular weight: 38.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R5TZM7|A0A4R5TZM7_9MICC 2'-5' RNA ligase family protein OS=Arthrobacter crusticola OX=2547960 GN=E2F48_06095 PE=4 SV=1
MM1 pKa = 7.69SNRR4 pKa = 11.84LINAAGRR11 pKa = 11.84LAGRR15 pKa = 11.84GTTGTTGTGRR25 pKa = 11.84TRR27 pKa = 11.84GTGGVGRR34 pKa = 11.84GMGRR38 pKa = 11.84TTGRR42 pKa = 11.84GGMSSGSRR50 pKa = 11.84GGLGGMIRR58 pKa = 11.84GILNRR63 pKa = 11.84RR64 pKa = 3.45

Molecular weight: 6.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3325

0

3325

1063883

32

1698

320.0

34.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.236 ± 0.071

0.574 ± 0.011

5.592 ± 0.033

5.87 ± 0.043

3.129 ± 0.028

9.4 ± 0.04

1.975 ± 0.02

4.156 ± 0.031

2.207 ± 0.031

10.523 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.799 ± 0.015

2.244 ± 0.026

5.728 ± 0.037

2.908 ± 0.023

7.159 ± 0.038

5.899 ± 0.028

5.838 ± 0.027

8.36 ± 0.041

1.386 ± 0.018

2.02 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski