Microbacterium phage Jayden

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Metamorphoovirus; unclassified Metamorphoovirus

Average proteome isoelectric point is 5.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 92 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A649VTJ4|A0A649VTJ4_9CAUD Uncharacterized protein OS=Microbacterium phage Jayden OX=2656550 GN=29 PE=4 SV=1
MM1 pKa = 7.17LTDD4 pKa = 4.31DD5 pKa = 4.71AAATSAAAFVVPTDD19 pKa = 3.7PPVTQACGWPVVYY32 pKa = 10.41LDD34 pKa = 5.48CADD37 pKa = 5.05DD38 pKa = 4.36DD39 pKa = 4.28CAAYY43 pKa = 10.4SQFPEE48 pKa = 4.3DD49 pKa = 3.36QQAAAQAWFEE59 pKa = 4.02AQAIDD64 pKa = 4.48ILWNATNGVFGVCDD78 pKa = 3.54VEE80 pKa = 5.31IRR82 pKa = 11.84PCRR85 pKa = 11.84QGCEE89 pKa = 4.48SNFDD93 pKa = 3.31WATTFWGRR101 pKa = 11.84GPGFSPGFPRR111 pKa = 11.84LGVGGAGGSFYY122 pKa = 10.48PVLVSGQWYY131 pKa = 9.81NVTCGCLGACQCSPSGPNVLSLPGPVVAVTEE162 pKa = 4.17VTIDD166 pKa = 3.62GLVVDD171 pKa = 4.8PATYY175 pKa = 10.33RR176 pKa = 11.84IDD178 pKa = 3.31RR179 pKa = 11.84GRR181 pKa = 11.84WLIRR185 pKa = 11.84DD186 pKa = 3.76GDD188 pKa = 4.31EE189 pKa = 4.36PWPGCQDD196 pKa = 3.28MNVPADD202 pKa = 3.58AVGSFVVRR210 pKa = 11.84YY211 pKa = 7.45TRR213 pKa = 11.84GIPVPAGGQIAAGRR227 pKa = 11.84LACEE231 pKa = 4.43LAMAACGDD239 pKa = 4.45DD240 pKa = 5.29DD241 pKa = 5.82CALPDD246 pKa = 3.18NWQTITRR253 pKa = 11.84QGLTVNADD261 pKa = 3.34PNMDD265 pKa = 3.55GTQVTGIWSIDD276 pKa = 3.07EE277 pKa = 4.27WIKK280 pKa = 10.35QVNRR284 pKa = 11.84PRR286 pKa = 11.84SFASVRR292 pKa = 11.84SVDD295 pKa = 4.24LPNLRR300 pKa = 4.34

Molecular weight: 31.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A649VSR4|A0A649VSR4_9CAUD Uncharacterized protein OS=Microbacterium phage Jayden OX=2656550 GN=34 PE=4 SV=1
MM1 pKa = 7.8TITTTTIPEE10 pKa = 4.03PVLEE14 pKa = 4.19VGRR17 pKa = 11.84AFEE20 pKa = 4.08KK21 pKa = 10.46RR22 pKa = 11.84RR23 pKa = 11.84EE24 pKa = 3.9IAQRR28 pKa = 11.84LADD31 pKa = 3.53QDD33 pKa = 3.98RR34 pKa = 11.84YY35 pKa = 10.52IGAAVRR41 pKa = 11.84TARR44 pKa = 11.84KK45 pKa = 9.49AGHH48 pKa = 6.06SWAEE52 pKa = 3.73MARR55 pKa = 11.84AAKK58 pKa = 10.32VSDD61 pKa = 3.6VAILKK66 pKa = 9.57AARR69 pKa = 11.84RR70 pKa = 11.84PEE72 pKa = 3.85KK73 pKa = 10.61AAA75 pKa = 3.79

Molecular weight: 8.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

92

0

92

16792

29

955

182.5

19.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.083 ± 0.404

0.923 ± 0.136

6.557 ± 0.335

6.539 ± 0.386

2.686 ± 0.115

8.802 ± 0.373

1.846 ± 0.193

4.413 ± 0.248

2.579 ± 0.227

7.462 ± 0.358

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.406 ± 0.156

2.555 ± 0.172

6.122 ± 0.267

3.013 ± 0.188

6.712 ± 0.387

6.164 ± 0.264

6.908 ± 0.366

7.641 ± 0.274

2.078 ± 0.156

2.513 ± 0.178
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski