Amino acid dipepetide frequency for Microbacterium phage Jayden

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.033AlaAla: 17.033 ± 1.639
0.893AlaCys: 0.893 ± 0.319
7.921AlaAsp: 7.921 ± 0.638
8.1AlaGlu: 8.1 ± 0.952
3.276AlaPhe: 3.276 ± 0.472
10.065AlaGly: 10.065 ± 0.952
2.382AlaHis: 2.382 ± 0.362
4.824AlaIle: 4.824 ± 0.583
3.335AlaLys: 3.335 ± 0.475
11.077AlaLeu: 11.077 ± 1.252
3.156AlaMet: 3.156 ± 0.46
2.68AlaAsn: 2.68 ± 0.534
5.896AlaPro: 5.896 ± 0.723
3.097AlaGln: 3.097 ± 0.365
8.04AlaArg: 8.04 ± 0.821
7.98AlaSer: 7.98 ± 0.646
7.921AlaThr: 7.921 ± 0.882
8.219AlaVal: 8.219 ± 0.84
2.74AlaTrp: 2.74 ± 0.442
2.204AlaTyr: 2.204 ± 0.43
0.0AlaXaa: 0.0 ± 0.0
Cys
1.132CysAla: 1.132 ± 0.313
0.238CysCys: 0.238 ± 0.152
1.132CysAsp: 1.132 ± 0.288
0.715CysGlu: 0.715 ± 0.243
0.179CysPhe: 0.179 ± 0.104
1.37CysGly: 1.37 ± 0.315
0.179CysHis: 0.179 ± 0.088
0.596CysIle: 0.596 ± 0.166
0.06CysLys: 0.06 ± 0.066
0.655CysLeu: 0.655 ± 0.185
0.119CysMet: 0.119 ± 0.088
0.238CysAsn: 0.238 ± 0.108
0.834CysPro: 0.834 ± 0.235
0.119CysGln: 0.119 ± 0.124
0.357CysArg: 0.357 ± 0.153
0.238CysSer: 0.238 ± 0.113
0.298CysThr: 0.298 ± 0.136
0.655CysVal: 0.655 ± 0.214
0.06CysTrp: 0.06 ± 0.063
0.06CysTyr: 0.06 ± 0.061
0.0CysXaa: 0.0 ± 0.0
Asp
7.861AspAla: 7.861 ± 0.801
0.715AspCys: 0.715 ± 0.247
5.241AspAsp: 5.241 ± 0.841
5.479AspGlu: 5.479 ± 0.851
2.025AspPhe: 2.025 ± 0.331
6.075AspGly: 6.075 ± 0.558
0.953AspHis: 0.953 ± 0.206
2.68AspIle: 2.68 ± 0.463
1.191AspLys: 1.191 ± 0.238
5.479AspLeu: 5.479 ± 0.681
1.429AspMet: 1.429 ± 0.292
1.965AspAsn: 1.965 ± 0.282
4.05AspPro: 4.05 ± 0.605
2.084AspGln: 2.084 ± 0.336
4.169AspArg: 4.169 ± 0.6
3.335AspSer: 3.335 ± 0.416
3.216AspThr: 3.216 ± 0.504
4.586AspVal: 4.586 ± 0.647
1.31AspTrp: 1.31 ± 0.3
1.727AspTyr: 1.727 ± 0.396
0.0AspXaa: 0.0 ± 0.0
Glu
8.278GluAla: 8.278 ± 0.83
0.536GluCys: 0.536 ± 0.196
3.812GluAsp: 3.812 ± 0.631
4.407GluGlu: 4.407 ± 0.591
2.144GluPhe: 2.144 ± 0.361
5.122GluGly: 5.122 ± 0.795
1.668GluHis: 1.668 ± 0.315
1.548GluIle: 1.548 ± 0.33
2.204GluLys: 2.204 ± 0.362
2.799GluLeu: 2.799 ± 0.419
1.132GluMet: 1.132 ± 0.286
1.548GluAsn: 1.548 ± 0.304
5.42GluPro: 5.42 ± 0.721
2.74GluGln: 2.74 ± 0.438
5.658GluArg: 5.658 ± 0.574
4.05GluSer: 4.05 ± 0.536
3.276GluThr: 3.276 ± 0.511
5.539GluVal: 5.539 ± 0.58
1.548GluTrp: 1.548 ± 0.301
1.965GluTyr: 1.965 ± 0.384
0.0GluXaa: 0.0 ± 0.0
Phe
3.156PheAla: 3.156 ± 0.437
0.417PheCys: 0.417 ± 0.153
2.442PheAsp: 2.442 ± 0.419
2.144PheGlu: 2.144 ± 0.447
0.476PhePhe: 0.476 ± 0.157
2.442PheGly: 2.442 ± 0.4
0.298PheHis: 0.298 ± 0.13
1.668PheIle: 1.668 ± 0.332
0.715PheLys: 0.715 ± 0.207
1.548PheLeu: 1.548 ± 0.403
0.596PheMet: 0.596 ± 0.179
0.476PheAsn: 0.476 ± 0.207
1.191PhePro: 1.191 ± 0.291
0.417PheGln: 0.417 ± 0.142
1.608PheArg: 1.608 ± 0.285
1.608PheSer: 1.608 ± 0.34
2.68PheThr: 2.68 ± 0.401
1.787PheVal: 1.787 ± 0.295
0.417PheTrp: 0.417 ± 0.153
0.774PheTyr: 0.774 ± 0.201
0.0PheXaa: 0.0 ± 0.0
Gly
9.112GlyAla: 9.112 ± 1.151
0.715GlyCys: 0.715 ± 0.253
6.372GlyAsp: 6.372 ± 0.875
5.181GlyGlu: 5.181 ± 0.684
3.156GlyPhe: 3.156 ± 0.405
7.861GlyGly: 7.861 ± 0.851
1.965GlyHis: 1.965 ± 0.299
4.228GlyIle: 4.228 ± 0.706
2.62GlyLys: 2.62 ± 0.386
7.087GlyLeu: 7.087 ± 0.67
3.395GlyMet: 3.395 ± 0.493
2.204GlyAsn: 2.204 ± 0.381
3.812GlyPro: 3.812 ± 0.424
2.68GlyGln: 2.68 ± 0.347
4.705GlyArg: 4.705 ± 0.664
5.479GlySer: 5.479 ± 0.617
6.789GlyThr: 6.789 ± 0.945
5.658GlyVal: 5.658 ± 0.549
2.501GlyTrp: 2.501 ± 0.352
2.501GlyTyr: 2.501 ± 0.422
0.0GlyXaa: 0.0 ± 0.0
His
1.846HisAla: 1.846 ± 0.299
0.179HisCys: 0.179 ± 0.094
1.608HisAsp: 1.608 ± 0.318
1.608HisGlu: 1.608 ± 0.339
0.715HisPhe: 0.715 ± 0.208
1.489HisGly: 1.489 ± 0.336
0.476HisHis: 0.476 ± 0.213
0.536HisIle: 0.536 ± 0.165
0.476HisLys: 0.476 ± 0.174
1.429HisLeu: 1.429 ± 0.301
0.357HisMet: 0.357 ± 0.14
0.179HisAsn: 0.179 ± 0.112
1.191HisPro: 1.191 ± 0.267
0.417HisGln: 0.417 ± 0.136
1.251HisArg: 1.251 ± 0.274
1.132HisSer: 1.132 ± 0.24
1.31HisThr: 1.31 ± 0.265
1.191HisVal: 1.191 ± 0.293
0.357HisTrp: 0.357 ± 0.173
0.715HisTyr: 0.715 ± 0.188
0.0HisXaa: 0.0 ± 0.0
Ile
4.526IleAla: 4.526 ± 0.457
0.476IleCys: 0.476 ± 0.179
3.216IleAsp: 3.216 ± 0.428
3.216IleGlu: 3.216 ± 0.391
0.596IlePhe: 0.596 ± 0.171
3.216IleGly: 3.216 ± 0.364
0.655IleHis: 0.655 ± 0.175
2.561IleIle: 2.561 ± 0.556
0.834IleLys: 0.834 ± 0.225
2.263IleLeu: 2.263 ± 0.348
0.834IleMet: 0.834 ± 0.255
1.191IleAsn: 1.191 ± 0.343
3.692IlePro: 3.692 ± 0.843
1.429IleGln: 1.429 ± 0.287
3.156IleArg: 3.156 ± 0.464
2.68IleSer: 2.68 ± 0.423
4.467IleThr: 4.467 ± 0.49
3.692IleVal: 3.692 ± 0.501
0.774IleTrp: 0.774 ± 0.236
0.655IleTyr: 0.655 ± 0.191
0.0IleXaa: 0.0 ± 0.0
Lys
3.276LysAla: 3.276 ± 0.542
0.357LysCys: 0.357 ± 0.144
1.31LysAsp: 1.31 ± 0.309
0.834LysGlu: 0.834 ± 0.216
0.655LysPhe: 0.655 ± 0.196
2.323LysGly: 2.323 ± 0.354
0.417LysHis: 0.417 ± 0.155
1.072LysIle: 1.072 ± 0.25
0.476LysLys: 0.476 ± 0.166
0.774LysLeu: 0.774 ± 0.261
0.476LysMet: 0.476 ± 0.138
0.596LysAsn: 0.596 ± 0.201
2.382LysPro: 2.382 ± 0.409
0.774LysGln: 0.774 ± 0.18
3.216LysArg: 3.216 ± 0.526
1.668LysSer: 1.668 ± 0.272
0.715LysThr: 0.715 ± 0.207
3.037LysVal: 3.037 ± 0.453
0.536LysTrp: 0.536 ± 0.172
0.893LysTyr: 0.893 ± 0.225
0.0LysXaa: 0.0 ± 0.0
Leu
9.529LeuAla: 9.529 ± 0.992
0.476LeuCys: 0.476 ± 0.189
5.777LeuAsp: 5.777 ± 0.668
4.288LeuGlu: 4.288 ± 0.51
1.489LeuPhe: 1.489 ± 0.38
6.492LeuGly: 6.492 ± 0.927
0.953LeuHis: 0.953 ± 0.228
3.99LeuIle: 3.99 ± 0.681
1.012LeuLys: 1.012 ± 0.27
5.777LeuLeu: 5.777 ± 0.716
1.429LeuMet: 1.429 ± 0.293
1.787LeuAsn: 1.787 ± 0.324
4.705LeuPro: 4.705 ± 0.428
1.787LeuGln: 1.787 ± 0.355
4.943LeuArg: 4.943 ± 0.615
5.241LeuSer: 5.241 ± 0.722
6.67LeuThr: 6.67 ± 0.523
5.539LeuVal: 5.539 ± 0.596
1.012LeuTrp: 1.012 ± 0.207
1.727LeuTyr: 1.727 ± 0.39
0.0LeuXaa: 0.0 ± 0.0
Met
3.276MetAla: 3.276 ± 0.479
0.119MetCys: 0.119 ± 0.132
1.37MetAsp: 1.37 ± 0.322
0.536MetGlu: 0.536 ± 0.201
0.596MetPhe: 0.596 ± 0.214
1.727MetGly: 1.727 ± 0.367
0.417MetHis: 0.417 ± 0.139
1.251MetIle: 1.251 ± 0.296
0.357MetLys: 0.357 ± 0.152
2.084MetLeu: 2.084 ± 0.393
0.417MetMet: 0.417 ± 0.182
0.655MetAsn: 0.655 ± 0.192
1.132MetPro: 1.132 ± 0.266
0.774MetGln: 0.774 ± 0.19
1.31MetArg: 1.31 ± 0.256
2.74MetSer: 2.74 ± 0.358
2.442MetThr: 2.442 ± 0.357
1.608MetVal: 1.608 ± 0.303
0.476MetTrp: 0.476 ± 0.149
0.774MetTyr: 0.774 ± 0.203
0.0MetXaa: 0.0 ± 0.0
Asn
3.99AsnAla: 3.99 ± 0.499
0.238AsnCys: 0.238 ± 0.112
1.191AsnAsp: 1.191 ± 0.253
0.893AsnGlu: 0.893 ± 0.223
0.417AsnPhe: 0.417 ± 0.158
2.859AsnGly: 2.859 ± 0.475
0.298AsnHis: 0.298 ± 0.156
1.012AsnIle: 1.012 ± 0.265
0.298AsnLys: 0.298 ± 0.129
2.501AsnLeu: 2.501 ± 0.329
0.357AsnMet: 0.357 ± 0.146
0.298AsnAsn: 0.298 ± 0.111
2.918AsnPro: 2.918 ± 0.554
0.596AsnGln: 0.596 ± 0.17
1.727AsnArg: 1.727 ± 0.316
1.489AsnSer: 1.489 ± 0.333
1.37AsnThr: 1.37 ± 0.277
2.084AsnVal: 2.084 ± 0.348
0.357AsnTrp: 0.357 ± 0.141
0.655AsnTyr: 0.655 ± 0.246
0.0AsnXaa: 0.0 ± 0.0
Pro
8.159ProAla: 8.159 ± 0.911
0.715ProCys: 0.715 ± 0.254
3.99ProAsp: 3.99 ± 0.778
5.36ProGlu: 5.36 ± 0.656
1.787ProPhe: 1.787 ± 0.267
5.36ProGly: 5.36 ± 0.661
1.489ProHis: 1.489 ± 0.32
1.906ProIle: 1.906 ± 0.34
1.846ProLys: 1.846 ± 0.357
3.99ProLeu: 3.99 ± 0.454
1.548ProMet: 1.548 ± 0.267
2.263ProAsn: 2.263 ± 0.427
3.395ProPro: 3.395 ± 0.534
2.204ProGln: 2.204 ± 0.481
2.74ProArg: 2.74 ± 0.472
3.395ProSer: 3.395 ± 0.568
4.109ProThr: 4.109 ± 0.736
4.526ProVal: 4.526 ± 0.616
1.132ProTrp: 1.132 ± 0.255
1.251ProTyr: 1.251 ± 0.179
0.0ProXaa: 0.0 ± 0.0
Gln
3.395GlnAla: 3.395 ± 0.544
0.298GlnCys: 0.298 ± 0.121
1.251GlnAsp: 1.251 ± 0.252
1.37GlnGlu: 1.37 ± 0.296
1.191GlnPhe: 1.191 ± 0.28
2.68GlnGly: 2.68 ± 0.338
0.596GlnHis: 0.596 ± 0.202
1.608GlnIle: 1.608 ± 0.297
0.953GlnLys: 0.953 ± 0.231
1.132GlnLeu: 1.132 ± 0.399
1.072GlnMet: 1.072 ± 0.224
1.072GlnAsn: 1.072 ± 0.258
1.846GlnPro: 1.846 ± 0.388
1.251GlnGln: 1.251 ± 0.286
2.442GlnArg: 2.442 ± 0.376
2.025GlnSer: 2.025 ± 0.391
1.727GlnThr: 1.727 ± 0.326
2.62GlnVal: 2.62 ± 0.416
0.715GlnTrp: 0.715 ± 0.238
0.893GlnTyr: 0.893 ± 0.189
0.0GlnXaa: 0.0 ± 0.0
Arg
6.551ArgAla: 6.551 ± 0.699
0.715ArgCys: 0.715 ± 0.227
4.169ArgAsp: 4.169 ± 0.683
4.169ArgGlu: 4.169 ± 0.568
1.965ArgPhe: 1.965 ± 0.322
6.194ArgGly: 6.194 ± 0.638
1.191ArgHis: 1.191 ± 0.346
2.263ArgIle: 2.263 ± 0.392
2.084ArgLys: 2.084 ± 0.339
6.372ArgLeu: 6.372 ± 0.633
1.906ArgMet: 1.906 ± 0.315
1.548ArgAsn: 1.548 ± 0.377
3.216ArgPro: 3.216 ± 0.53
2.144ArgGln: 2.144 ± 0.405
5.598ArgArg: 5.598 ± 0.789
3.633ArgSer: 3.633 ± 0.513
4.169ArgThr: 4.169 ± 0.51
5.003ArgVal: 5.003 ± 0.587
1.846ArgTrp: 1.846 ± 0.279
2.382ArgTyr: 2.382 ± 0.456
0.0ArgXaa: 0.0 ± 0.0
Ser
7.564SerAla: 7.564 ± 1.028
0.238SerCys: 0.238 ± 0.122
3.692SerAsp: 3.692 ± 0.532
4.109SerGlu: 4.109 ± 0.535
1.489SerPhe: 1.489 ± 0.38
5.896SerGly: 5.896 ± 0.723
1.012SerHis: 1.012 ± 0.232
3.037SerIle: 3.037 ± 0.649
1.548SerLys: 1.548 ± 0.291
4.645SerLeu: 4.645 ± 0.453
2.025SerMet: 2.025 ± 0.304
1.965SerAsn: 1.965 ± 0.34
2.978SerPro: 2.978 ± 0.408
1.668SerGln: 1.668 ± 0.352
3.931SerArg: 3.931 ± 0.522
3.156SerSer: 3.156 ± 0.668
5.181SerThr: 5.181 ± 0.636
4.586SerVal: 4.586 ± 0.508
1.251SerTrp: 1.251 ± 0.331
1.668SerTyr: 1.668 ± 0.3
0.0SerXaa: 0.0 ± 0.0
Thr
9.052ThrAla: 9.052 ± 1.053
0.774ThrCys: 0.774 ± 0.205
4.169ThrAsp: 4.169 ± 0.473
4.228ThrGlu: 4.228 ± 0.501
1.846ThrPhe: 1.846 ± 0.272
6.492ThrGly: 6.492 ± 0.922
1.191ThrHis: 1.191 ± 0.252
3.514ThrIle: 3.514 ± 0.588
2.144ThrLys: 2.144 ± 0.372
5.241ThrLeu: 5.241 ± 0.601
1.37ThrMet: 1.37 ± 0.296
1.548ThrAsn: 1.548 ± 0.308
5.896ThrPro: 5.896 ± 0.804
1.787ThrGln: 1.787 ± 0.28
3.633ThrArg: 3.633 ± 0.417
3.514ThrSer: 3.514 ± 0.443
3.931ThrThr: 3.931 ± 0.805
6.194ThrVal: 6.194 ± 0.713
1.012ThrTrp: 1.012 ± 0.218
1.548ThrTyr: 1.548 ± 0.27
0.0ThrXaa: 0.0 ± 0.0
Val
8.516ValAla: 8.516 ± 0.675
0.655ValCys: 0.655 ± 0.21
3.335ValAsp: 3.335 ± 0.486
5.539ValGlu: 5.539 ± 0.577
1.548ValPhe: 1.548 ± 0.307
6.67ValGly: 6.67 ± 0.591
1.429ValHis: 1.429 ± 0.319
4.169ValIle: 4.169 ± 0.484
2.263ValLys: 2.263 ± 0.406
6.313ValLeu: 6.313 ± 0.563
1.31ValMet: 1.31 ± 0.254
2.144ValAsn: 2.144 ± 0.389
4.705ValPro: 4.705 ± 0.534
2.442ValGln: 2.442 ± 0.399
5.062ValArg: 5.062 ± 0.525
5.42ValSer: 5.42 ± 0.586
6.134ValThr: 6.134 ± 0.622
5.717ValVal: 5.717 ± 0.656
1.548ValTrp: 1.548 ± 0.334
1.489ValTyr: 1.489 ± 0.268
0.0ValXaa: 0.0 ± 0.0
Trp
2.263TrpAla: 2.263 ± 0.392
0.357TrpCys: 0.357 ± 0.146
1.072TrpAsp: 1.072 ± 0.225
1.608TrpGlu: 1.608 ± 0.418
0.834TrpPhe: 0.834 ± 0.222
1.668TrpGly: 1.668 ± 0.298
0.536TrpHis: 0.536 ± 0.176
0.953TrpIle: 0.953 ± 0.261
0.774TrpLys: 0.774 ± 0.216
1.37TrpLeu: 1.37 ± 0.277
0.417TrpMet: 0.417 ± 0.164
0.715TrpAsn: 0.715 ± 0.209
0.715TrpPro: 0.715 ± 0.226
0.715TrpGln: 0.715 ± 0.215
1.191TrpArg: 1.191 ± 0.245
1.489TrpSer: 1.489 ± 0.316
1.072TrpThr: 1.072 ± 0.245
1.429TrpVal: 1.429 ± 0.294
0.834TrpTrp: 0.834 ± 0.239
0.774TrpTyr: 0.774 ± 0.219
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.323TyrAla: 2.323 ± 0.36
0.119TyrCys: 0.119 ± 0.082
2.501TyrAsp: 2.501 ± 0.452
1.608TyrGlu: 1.608 ± 0.313
0.476TyrPhe: 0.476 ± 0.174
2.025TyrGly: 2.025 ± 0.32
0.357TyrHis: 0.357 ± 0.14
0.715TyrIle: 0.715 ± 0.173
0.596TyrLys: 0.596 ± 0.174
2.084TyrLeu: 2.084 ± 0.365
0.655TyrMet: 0.655 ± 0.207
0.476TyrAsn: 0.476 ± 0.147
1.251TyrPro: 1.251 ± 0.267
1.012TyrGln: 1.012 ± 0.259
2.382TyrArg: 2.382 ± 0.353
1.37TyrSer: 1.37 ± 0.252
1.608TyrThr: 1.608 ± 0.283
2.74TyrVal: 2.74 ± 0.458
0.357TyrTrp: 0.357 ± 0.147
0.476TyrTyr: 0.476 ± 0.164
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 92 proteins (16792 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski