Treponema socranskii subsp. socranskii VPI DR56BR1116 = ATCC 35536
Average proteome isoelectric point is 6.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2624 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|U1FMJ2|U1FMJ2_TRESO Fcf1 OS=Treponema socranskii subsp. socranskii VPI DR56BR1116 = ATCC 35536 OX=1125725 GN=HMPREF1325_1651 PE=3 SV=1
MM1 pKa = 8.05 DD2 pKa = 5.19 KK3 pKa = 11.22 YY4 pKa = 10.8 EE5 pKa = 4.4 CTVCGYY11 pKa = 11.02 VYY13 pKa = 10.54 DD14 pKa = 4.42 PEE16 pKa = 6.53 KK17 pKa = 10.82 GDD19 pKa = 3.89 PDD21 pKa = 3.92 GGIAPGTKK29 pKa = 9.04 WEE31 pKa = 5.71 DD32 pKa = 3.46 IPDD35 pKa = 3.65 DD36 pKa = 4.33 WVCPLCGVGKK46 pKa = 10.35 DD47 pKa = 3.69 SFQKK51 pKa = 10.12 QQ52 pKa = 2.9
Molecular weight: 5.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.807
IPC2_protein 4.037
IPC_protein 3.935
Toseland 3.732
ProMoST 4.126
Dawson 3.948
Bjellqvist 4.113
Wikipedia 3.923
Rodwell 3.77
Grimsley 3.643
Solomon 3.935
Lehninger 3.884
Nozaki 4.075
DTASelect 4.329
Thurlkill 3.808
EMBOSS 3.923
Sillero 4.062
Patrickios 0.782
IPC_peptide 3.923
IPC2_peptide 4.037
IPC2.peptide.svr19 3.955
Protein with the highest isoelectric point:
>tr|U2MP12|U2MP12_TRESO Periplasmic-binding protein domain protein OS=Treponema socranskii subsp. socranskii VPI DR56BR1116 = ATCC 35536 OX=1125725 GN=HMPREF1325_0476 PE=4 SV=1
MM1 pKa = 6.58 VTSKK5 pKa = 10.91 KK6 pKa = 10.66 LSGTAALIFFIYY18 pKa = 8.82 CTIALSQNARR28 pKa = 11.84 DD29 pKa = 3.92 PKK31 pKa = 9.89 GARR34 pKa = 11.84 PYY36 pKa = 10.55 RR37 pKa = 11.84 SMRR40 pKa = 11.84 MRR42 pKa = 11.84 AVIRR46 pKa = 11.84 RR47 pKa = 11.84 NEE49 pKa = 3.59 AHH51 pKa = 6.36 EE52 pKa = 4.2 RR53 pKa = 11.84 SGGGAWDD60 pKa = 3.41 WDD62 pKa = 3.95 CRR64 pKa = 11.84 SSKK67 pKa = 11.0 ALL69 pKa = 3.49
Molecular weight: 7.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.348
IPC2_protein 9.692
IPC_protein 10.57
Toseland 10.745
ProMoST 10.482
Dawson 10.833
Bjellqvist 10.57
Wikipedia 11.067
Rodwell 11.008
Grimsley 10.877
Solomon 10.979
Lehninger 10.95
Nozaki 10.745
DTASelect 10.555
Thurlkill 10.745
EMBOSS 11.155
Sillero 10.774
Patrickios 10.804
IPC_peptide 10.994
IPC2_peptide 9.765
IPC2.peptide.svr19 8.524
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2624
0
2624
852725
33
2972
325.0
35.94
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.61 ± 0.058
1.419 ± 0.022
5.646 ± 0.038
6.157 ± 0.048
4.742 ± 0.038
7.095 ± 0.049
1.554 ± 0.018
7.097 ± 0.039
6.646 ± 0.047
8.743 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.477 ± 0.023
3.962 ± 0.03
3.683 ± 0.031
2.603 ± 0.026
4.898 ± 0.038
6.708 ± 0.044
5.65 ± 0.044
6.812 ± 0.041
0.912 ± 0.014
3.584 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here