Sarcoptes scabiei (Itch mite) (Acarus scabiei)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda;

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10528 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A132AFI1|A0A132AFI1_SARSC GPN-loop GTPase 2 (Fragment) OS=Sarcoptes scabiei OX=52283 GN=QR98_0077230 PE=3 SV=1
MM1 pKa = 7.79GIFFVVTNRR10 pKa = 11.84KK11 pKa = 8.85KK12 pKa = 9.89WISSDD17 pKa = 3.06FDD19 pKa = 3.92NYY21 pKa = 10.74TIDD24 pKa = 3.16IDD26 pKa = 3.57EE27 pKa = 5.54DD28 pKa = 3.77YY29 pKa = 10.83DD30 pKa = 3.93YY31 pKa = 11.05DD32 pKa = 4.12YY33 pKa = 11.05DD34 pKa = 4.1YY35 pKa = 11.67VDD37 pKa = 3.94VIVRR41 pKa = 11.84CHH43 pKa = 6.91DD44 pKa = 3.7DD45 pKa = 4.08SYY47 pKa = 12.05DD48 pKa = 3.51SDD50 pKa = 3.81GVMNLKK56 pKa = 9.96MMII59 pKa = 3.86

Molecular weight: 7.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A132AJ24|A0A132AJ24_SARSC Replication protein A subunit OS=Sarcoptes scabiei OX=52283 GN=QR98_0093820 PE=3 SV=1
MM1 pKa = 7.26MMMMMRR7 pKa = 11.84RR8 pKa = 11.84RR9 pKa = 11.84RR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84NN16 pKa = 3.02

Molecular weight: 2.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10527

1

10528

4005944

8

9023

380.5

43.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.62 ± 0.022

1.833 ± 0.024

5.932 ± 0.022

6.233 ± 0.029

4.781 ± 0.022

4.403 ± 0.029

2.492 ± 0.012

7.65 ± 0.024

6.795 ± 0.027

9.241 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.227 ± 0.012

6.946 ± 0.031

4.132 ± 0.027

4.539 ± 0.021

5.01 ± 0.018

9.311 ± 0.038

5.013 ± 0.021

4.676 ± 0.019

0.953 ± 0.009

3.21 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski