Mesorhizobium hungaricum
Average proteome isoelectric point is 6.68
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5848 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1C2DS55|A0A1C2DS55_9RHIZ Uncharacterized protein OS=Mesorhizobium hungaricum OX=1566387 GN=QV13_12710 PE=4 SV=1
MM1 pKa = 7.9 PDD3 pKa = 3.47 DD4 pKa = 4.04 TEE6 pKa = 4.6 HH7 pKa = 7.27 LVTACADD14 pKa = 3.59 FASKK18 pKa = 10.54 HH19 pKa = 6.29 GIDD22 pKa = 4.62 AMLDD26 pKa = 3.51 VLAGVYY32 pKa = 7.89 QVYY35 pKa = 10.41 APLSEE40 pKa = 4.42 DD41 pKa = 3.61 VAIKK45 pKa = 10.2 IAWDD49 pKa = 3.19 IASGSDD55 pKa = 3.21 VSVVTEE61 pKa = 4.08 IDD63 pKa = 3.15 SDD65 pKa = 4.24 GNATHH70 pKa = 6.6 HH71 pKa = 5.79 TVQQ74 pKa = 3.9
Molecular weight: 7.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.767
IPC2_protein 3.973
IPC_protein 3.923
Toseland 3.706
ProMoST 4.113
Dawson 3.935
Bjellqvist 4.088
Wikipedia 3.91
Rodwell 3.757
Grimsley 3.617
Solomon 3.923
Lehninger 3.884
Nozaki 4.062
DTASelect 4.342
Thurlkill 3.783
EMBOSS 3.923
Sillero 4.05
Patrickios 1.952
IPC_peptide 3.91
IPC2_peptide 4.024
IPC2.peptide.svr19 3.932
Protein with the highest isoelectric point:
>tr|A0A1C2DEH6|A0A1C2DEH6_9RHIZ ParB domain-containing protein OS=Mesorhizobium hungaricum OX=1566387 GN=QV13_26925 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.27 QPSKK9 pKa = 9.73 LVRR12 pKa = 11.84 KK13 pKa = 9.15 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.11 GGRR28 pKa = 11.84 GVVAARR34 pKa = 11.84 RR35 pKa = 11.84 NRR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.03 RR41 pKa = 11.84 LSAA44 pKa = 4.03
Molecular weight: 5.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.448
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.384
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.106
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.089
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5848
0
5848
1817757
27
3304
310.8
33.69
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.611 ± 0.048
0.774 ± 0.01
5.614 ± 0.028
5.522 ± 0.03
3.881 ± 0.021
8.638 ± 0.038
1.961 ± 0.015
5.277 ± 0.021
3.678 ± 0.028
9.98 ± 0.042
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.47 ± 0.016
2.708 ± 0.021
5.01 ± 0.032
3.053 ± 0.021
6.849 ± 0.036
5.443 ± 0.024
5.359 ± 0.032
7.545 ± 0.025
1.361 ± 0.013
2.266 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here