Feline morbillivirus
Average proteome isoelectric point is 6.63
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A140JPI2|A0A140JPI2_9MONO Phosphoprotein OS=Feline morbillivirus OX=1170234 GN=C PE=3 SV=1
MM1 pKa = 7.8 SSLLKK6 pKa = 10.74 SLAAFKK12 pKa = 10.38 RR13 pKa = 11.84 HH14 pKa = 6.06 RR15 pKa = 11.84 EE16 pKa = 4.0 QPTTPSGSGGTIKK29 pKa = 10.66 GLKK32 pKa = 7.85 NTIIVPVPGDD42 pKa = 3.53 TVITTRR48 pKa = 11.84 SNLLFRR54 pKa = 11.84 LVYY57 pKa = 10.45 IIGNPDD63 pKa = 3.36 TPLSTSTGAIISLLTLFVEE82 pKa = 5.4 SPGQLIQRR90 pKa = 11.84 IADD93 pKa = 3.88 DD94 pKa = 4.03 PDD96 pKa = 4.0 AVFKK100 pKa = 10.76 LVEE103 pKa = 4.44 VIPEE107 pKa = 4.14 AGNPGEE113 pKa = 4.21 LTFASRR119 pKa = 11.84 GINLDD124 pKa = 3.37 KK125 pKa = 10.87 QAQQYY130 pKa = 9.55 FKK132 pKa = 10.82 LAEE135 pKa = 4.43 KK136 pKa = 10.36 NDD138 pKa = 3.25 QGYY141 pKa = 8.7 YY142 pKa = 9.82 VSLGFEE148 pKa = 4.4 NPPNDD153 pKa = 4.91 DD154 pKa = 5.89 DD155 pKa = 3.91 ITSSPEE161 pKa = 3.18 IFNYY165 pKa = 9.98 ILASVLAQVWILLAKK180 pKa = 10.35 AVTAPDD186 pKa = 3.45 TAAEE190 pKa = 4.17 AEE192 pKa = 3.92 NRR194 pKa = 11.84 RR195 pKa = 11.84 WIKK198 pKa = 10.6 LMQQRR203 pKa = 11.84 RR204 pKa = 11.84 VDD206 pKa = 3.47 GEE208 pKa = 4.1 LRR210 pKa = 11.84 LSKK213 pKa = 10.63 GWLDD217 pKa = 3.45 LVRR220 pKa = 11.84 NKK222 pKa = 9.9 IASDD226 pKa = 2.99 ITIRR230 pKa = 11.84 RR231 pKa = 11.84 FMVALVLDD239 pKa = 4.53 IKK241 pKa = 10.5 RR242 pKa = 11.84 SPGTRR247 pKa = 11.84 PRR249 pKa = 11.84 IAEE252 pKa = 4.48 MICDD256 pKa = 3.28 IDD258 pKa = 4.08 NYY260 pKa = 10.18 IVEE263 pKa = 4.83 AGLASFLLTIKK274 pKa = 10.65 FGIEE278 pKa = 3.37 TRR280 pKa = 11.84 YY281 pKa = 8.99 PALALHH287 pKa = 6.46 EE288 pKa = 4.52 FSGEE292 pKa = 3.78 LATIEE297 pKa = 4.52 GLMKK301 pKa = 10.46 LYY303 pKa = 10.96 QSMGEE308 pKa = 3.9 MAPYY312 pKa = 9.55 MVILEE317 pKa = 4.26 NSIQTRR323 pKa = 11.84 FSAGSYY329 pKa = 9.24 PLLWSYY335 pKa = 12.06 AMGVGVEE342 pKa = 4.78 LEE344 pKa = 3.89 RR345 pKa = 11.84 SMGGLNFTRR354 pKa = 11.84 SFFDD358 pKa = 2.93 PTYY361 pKa = 10.54 FRR363 pKa = 11.84 LGQEE367 pKa = 3.89 MVRR370 pKa = 11.84 RR371 pKa = 11.84 SSGMVNSSFARR382 pKa = 11.84 EE383 pKa = 4.04 LGLSEE388 pKa = 5.92 HH389 pKa = 5.9 EE390 pKa = 4.35 TQLVSQIVNSGGEE403 pKa = 3.91 SGIPKK408 pKa = 9.92 FDD410 pKa = 3.59 GFRR413 pKa = 11.84 ANPTTFLGTKK423 pKa = 10.38 DD424 pKa = 3.88 NINDD428 pKa = 3.76 RR429 pKa = 11.84 GEE431 pKa = 4.36 DD432 pKa = 3.53 QSSSISGLPGPLLPSRR448 pKa = 11.84 DD449 pKa = 3.54 LTHH452 pKa = 7.24 PGDD455 pKa = 4.97 SYY457 pKa = 11.55 GTDD460 pKa = 2.97 NGVKK464 pKa = 10.27 DD465 pKa = 3.88 VSNKK469 pKa = 9.81 LNEE472 pKa = 5.28 GISPDD477 pKa = 3.59 HH478 pKa = 6.9 DD479 pKa = 4.13 VSSSAMEE486 pKa = 3.85 EE487 pKa = 3.79 LRR489 pKa = 11.84 RR490 pKa = 11.84 LVEE493 pKa = 3.88 STNRR497 pKa = 11.84 IDD499 pKa = 3.74 TKK501 pKa = 10.83 KK502 pKa = 10.39 PEE504 pKa = 4.27 APGVTNHH511 pKa = 6.17 YY512 pKa = 10.8 NDD514 pKa = 3.62 TDD516 pKa = 3.99 LLRR519 pKa = 5.68
Molecular weight: 57.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.238
IPC2_protein 5.27
IPC_protein 5.219
Toseland 5.181
ProMoST 5.372
Dawson 5.219
Bjellqvist 5.347
Wikipedia 5.105
Rodwell 5.143
Grimsley 5.118
Solomon 5.219
Lehninger 5.181
Nozaki 5.347
DTASelect 5.512
Thurlkill 5.181
EMBOSS 5.156
Sillero 5.423
Patrickios 4.393
IPC_peptide 5.232
IPC2_peptide 5.41
IPC2.peptide.svr19 5.456
Protein with the highest isoelectric point:
>tr|A0A140JPI4|A0A140JPI4_9MONO Fusion glycoprotein F0 OS=Feline morbillivirus OX=1170234 GN=F PE=3 SV=1
MM1 pKa = 7.46 TEE3 pKa = 4.17 IFTLDD8 pKa = 3.24 EE9 pKa = 4.51 SSWSIKK15 pKa = 8.92 GTLDD19 pKa = 3.54 PLTPDD24 pKa = 3.57 IYY26 pKa = 10.54 PDD28 pKa = 3.29 GRR30 pKa = 11.84 LVPKK34 pKa = 10.47 VRR36 pKa = 11.84 VIDD39 pKa = 4.1 PGLGDD44 pKa = 3.95 RR45 pKa = 11.84 KK46 pKa = 10.58 SGGYY50 pKa = 8.65 MYY52 pKa = 11.22 LLLHH56 pKa = 6.19 GVIEE60 pKa = 4.8 DD61 pKa = 4.13 SEE63 pKa = 4.52 NMSSPKK69 pKa = 10.13 GRR71 pKa = 11.84 AFGAFPLGVGQSTEE85 pKa = 3.93 NPEE88 pKa = 4.51 DD89 pKa = 3.53 LFKK92 pKa = 11.03 EE93 pKa = 3.94 ILTLNIVTRR102 pKa = 11.84 RR103 pKa = 11.84 TAGFNEE109 pKa = 3.63 KK110 pKa = 10.07 LVYY113 pKa = 11.04 YY114 pKa = 8.01 NTTPIHH120 pKa = 7.11 LLTPWKK126 pKa = 10.22 KK127 pKa = 8.77 VLAYY131 pKa = 10.37 GSIFNANQVCSDD143 pKa = 3.69 TSSIPIDD150 pKa = 3.34 IPQKK154 pKa = 10.09 FRR156 pKa = 11.84 PVYY159 pKa = 9.48 LTVTKK164 pKa = 10.82 LSDD167 pKa = 3.38 DD168 pKa = 4.07 GYY170 pKa = 9.35 YY171 pKa = 10.04 QIPKK175 pKa = 9.12 MIQDD179 pKa = 4.45 FKK181 pKa = 11.12 SSNSVAFNILVHH193 pKa = 7.52 LSMGTNLLDD202 pKa = 3.35 QSKK205 pKa = 10.93 DD206 pKa = 3.25 SRR208 pKa = 11.84 LRR210 pKa = 11.84 NAGEE214 pKa = 4.02 TVITFMIHH222 pKa = 5.52 IGNFKK227 pKa = 10.4 RR228 pKa = 11.84 KK229 pKa = 8.85 SNKK232 pKa = 9.53 SYY234 pKa = 10.77 SPEE237 pKa = 3.75 YY238 pKa = 9.97 CKK240 pKa = 10.6 RR241 pKa = 11.84 KK242 pKa = 9.75 IMRR245 pKa = 11.84 LGLIFSLGAIGGTSLHH261 pKa = 6.23 IRR263 pKa = 11.84 CTGKK267 pKa = 9.38 MSKK270 pKa = 10.24 RR271 pKa = 11.84 LQAYY275 pKa = 10.17 LGFKK279 pKa = 9.46 RR280 pKa = 11.84 TLCYY284 pKa = 10.3 PLMYY288 pKa = 10.74 VNEE291 pKa = 4.56 GLNKK295 pKa = 8.87 TLWRR299 pKa = 11.84 NEE301 pKa = 3.43 CRR303 pKa = 11.84 IEE305 pKa = 4.04 KK306 pKa = 9.52 VQAVLQPSVPNEE318 pKa = 3.57 FKK320 pKa = 11.15 VYY322 pKa = 10.79 DD323 pKa = 4.31 DD324 pKa = 5.14 VIIDD328 pKa = 3.66 NTNGLFKK335 pKa = 10.68 IKK337 pKa = 10.6
Molecular weight: 38.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.716
IPC2_protein 8.653
IPC_protein 8.551
Toseland 9.165
ProMoST 9.092
Dawson 9.487
Bjellqvist 9.282
Wikipedia 9.663
Rodwell 9.706
Grimsley 9.56
Solomon 9.545
Lehninger 9.502
Nozaki 9.37
DTASelect 9.224
Thurlkill 9.341
EMBOSS 9.633
Sillero 9.472
Patrickios 4.622
IPC_peptide 9.531
IPC2_peptide 7.936
IPC2.peptide.svr19 7.734
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
4687
337
2202
781.2
88.37
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.331 ± 0.35
1.664 ± 0.317
5.547 ± 0.368
4.95 ± 0.368
3.563 ± 0.464
5.291 ± 0.687
2.006 ± 0.293
9.75 ± 1.26
5.654 ± 0.42
10.39 ± 0.527
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.39 ± 0.102
5.867 ± 0.442
4.31 ± 0.363
3.627 ± 0.184
4.865 ± 0.371
9.345 ± 0.732
6.059 ± 0.239
5.035 ± 0.181
0.981 ± 0.157
4.374 ± 0.774
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here