Hop latent virus
Average proteome isoelectric point is 7.01
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q9E9R6|Q9E9R6_9VIRU Helicase OS=Hop latent virus OX=104263 GN=ORF 1 PE=2 SV=1
MM1 pKa = 7.86 DD2 pKa = 3.94 VLVDD6 pKa = 4.35 LLHH9 pKa = 6.65 KK10 pKa = 10.75 FGFEE14 pKa = 3.96 RR15 pKa = 11.84 LSSRR19 pKa = 11.84 LTTPIVVHH27 pKa = 6.13 CVPGAGKK34 pKa = 8.46 STLIRR39 pKa = 11.84 EE40 pKa = 4.43 LLDD43 pKa = 3.98 LDD45 pKa = 4.22 TRR47 pKa = 11.84 FCAYY51 pKa = 8.71 TAGVPDD57 pKa = 3.87 SPRR60 pKa = 11.84 LNGRR64 pKa = 11.84 WIRR67 pKa = 11.84 ALEE70 pKa = 4.29 EE71 pKa = 4.17 YY72 pKa = 10.13 PGTEE76 pKa = 3.54 GRR78 pKa = 11.84 LAIVDD83 pKa = 4.46 EE84 pKa = 4.28 YY85 pKa = 11.44 TLLDD89 pKa = 4.03 KK90 pKa = 11.46 LPFEE94 pKa = 4.63 PFAVFGDD101 pKa = 4.69 PIQSNSKK108 pKa = 10.26 GVLPAHH114 pKa = 6.18 FTCNFSRR121 pKa = 11.84 RR122 pKa = 11.84 FGLATSNLLRR132 pKa = 11.84 DD133 pKa = 4.13 LGWNVIAEE141 pKa = 4.42 GSDD144 pKa = 3.29 VVQISDD150 pKa = 3.19 IFGVEE155 pKa = 4.17 PIGTVVYY162 pKa = 9.49 FEE164 pKa = 5.38 AEE166 pKa = 4.27 VGCLLRR172 pKa = 11.84 SHH174 pKa = 6.51 CVEE177 pKa = 4.08 AKK179 pKa = 10.09 SLAEE183 pKa = 3.76 IRR185 pKa = 11.84 GQTFDD190 pKa = 2.77 IVTFVTSEE198 pKa = 3.98 NCPSSDD204 pKa = 2.85 VCAAFQCLTRR214 pKa = 11.84 HH215 pKa = 6.06 RR216 pKa = 11.84 EE217 pKa = 3.98 ALHH220 pKa = 5.88 ILCPNATYY228 pKa = 8.9 TAAA231 pKa = 4.37
Molecular weight: 25.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.048
IPC2_protein 5.156
IPC_protein 5.092
Toseland 5.092
ProMoST 5.258
Dawson 5.118
Bjellqvist 5.232
Wikipedia 5.016
Rodwell 5.041
Grimsley 5.029
Solomon 5.118
Lehninger 5.08
Nozaki 5.258
DTASelect 5.41
Thurlkill 5.13
EMBOSS 5.092
Sillero 5.334
Patrickios 3.706
IPC_peptide 5.13
IPC2_peptide 5.334
IPC2.peptide.svr19 5.287
Protein with the highest isoelectric point:
>tr|Q9E9R2|Q9E9R2_9VIRU Capsid protein OS=Hop latent virus OX=104263 GN=ORF 5 PE=2 SV=1
MM1 pKa = 7.5 FYY3 pKa = 11.05 LRR5 pKa = 11.84 VALLLHH11 pKa = 6.31 NKK13 pKa = 9.38 FLEE16 pKa = 4.04 QCGRR20 pKa = 11.84 SDD22 pKa = 3.29 FHH24 pKa = 8.21 LCVMISLQVHH34 pKa = 5.73 RR35 pKa = 11.84 PVGVGRR41 pKa = 11.84 SSYY44 pKa = 10.59 ARR46 pKa = 11.84 RR47 pKa = 11.84 RR48 pKa = 11.84 RR49 pKa = 11.84 AKK51 pKa = 10.48 LVGRR55 pKa = 11.84 CHH57 pKa = 5.96 RR58 pKa = 11.84 CYY60 pKa = 10.69 RR61 pKa = 11.84 LWPPTAFTTRR71 pKa = 11.84 CDD73 pKa = 3.54 NKK75 pKa = 9.85 TCFPGLTYY83 pKa = 10.4 NASIARR89 pKa = 11.84 FIRR92 pKa = 11.84 DD93 pKa = 3.03 GVTEE97 pKa = 4.74 VIPSAPNN104 pKa = 3.05
Molecular weight: 11.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.335
IPC2_protein 9.648
IPC_protein 10.584
Toseland 10.35
ProMoST 10.218
Dawson 10.555
Bjellqvist 10.379
Wikipedia 10.818
Rodwell 10.613
Grimsley 10.643
Solomon 10.657
Lehninger 10.613
Nozaki 10.482
DTASelect 10.335
Thurlkill 10.423
EMBOSS 10.774
Sillero 10.511
Patrickios 10.292
IPC_peptide 10.657
IPC2_peptide 9.882
IPC2.peptide.svr19 8.34
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2790
60
1981
465.0
52.38
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.814 ± 0.43
2.903 ± 0.566
5.09 ± 0.671
6.703 ± 1.025
4.695 ± 0.829
6.093 ± 0.584
2.473 ± 0.42
4.194 ± 0.267
5.806 ± 1.055
11.075 ± 0.748
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.971 ± 0.419
3.943 ± 0.515
4.301 ± 0.791
3.011 ± 0.367
6.667 ± 0.726
6.953 ± 0.324
4.194 ± 0.624
7.563 ± 0.796
1.29 ± 0.128
3.262 ± 0.382
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here