Maribacter vaceletii
Average proteome isoelectric point is 6.74
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3317 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A495E6A9|A0A495E6A9_9FLAO Uncharacterized protein OS=Maribacter vaceletii OX=1206816 GN=CLV91_2581 PE=4 SV=1
MM1 pKa = 7.61 KK2 pKa = 10.44 KK3 pKa = 8.44 ITNTKK8 pKa = 7.68 YY9 pKa = 10.41 VKK11 pKa = 10.68 NIFASALMLVATTSTFAQDD30 pKa = 2.98 EE31 pKa = 4.39 EE32 pKa = 4.46 AAEE35 pKa = 4.08 EE36 pKa = 4.15 APKK39 pKa = 11.0 LEE41 pKa = 4.03 ISGSVDD47 pKa = 2.63 AYY49 pKa = 11.2 YY50 pKa = 9.01 RR51 pKa = 11.84 TAFNDD56 pKa = 3.24 AGVASTDD63 pKa = 3.38 PGTAFANQTGFALGMGNLIASYY85 pKa = 10.91 DD86 pKa = 3.64 AGKK89 pKa = 10.66 VGAVLDD95 pKa = 3.89 IVVGPRR101 pKa = 11.84 GAGATFNTDD110 pKa = 2.52 IVDD113 pKa = 4.88 GIVNQAYY120 pKa = 9.75 VYY122 pKa = 10.42 WNVSDD127 pKa = 3.83 KK128 pKa = 8.92 TTLTMGRR135 pKa = 11.84 FNTFLGYY142 pKa = 10.2 EE143 pKa = 4.37 VIAPQANFNYY153 pKa = 8.18 STSYY157 pKa = 10.84 LFSNGPFSHH166 pKa = 6.72 MGLKK170 pKa = 10.22 ADD172 pKa = 4.86 FALSDD177 pKa = 4.42 DD178 pKa = 3.89 FSLMVGVMNPWDD190 pKa = 3.78 TNDD193 pKa = 2.92 ISGTGEE199 pKa = 3.95 YY200 pKa = 10.75 SFGAQLGFYY209 pKa = 9.73 GQYY212 pKa = 11.08 LNFYY216 pKa = 9.24 YY217 pKa = 10.59 DD218 pKa = 3.09 SGKK221 pKa = 10.5 NGGLGTEE228 pKa = 3.96 IDD230 pKa = 3.8 FTGGFDD236 pKa = 3.32 ISDD239 pKa = 3.55 AFFLGINAAYY249 pKa = 7.5 DD250 pKa = 3.68 TNDD253 pKa = 3.38 AADD256 pKa = 3.32 TGFYY260 pKa = 10.66 GVALYY265 pKa = 8.64 PQIATSDD272 pKa = 3.37 AFSIGLRR279 pKa = 11.84 GEE281 pKa = 4.39 YY282 pKa = 9.46 FASTIDD288 pKa = 4.14 GGDD291 pKa = 3.41 NYY293 pKa = 11.51 DD294 pKa = 4.3 DD295 pKa = 3.58 VTAITLTGSYY305 pKa = 10.59 SVDD308 pKa = 3.1 NLILKK313 pKa = 10.13 PEE315 pKa = 3.99 FRR317 pKa = 11.84 LDD319 pKa = 3.75 SIKK322 pKa = 11.17 DD323 pKa = 3.48 GFTDD327 pKa = 3.34 SDD329 pKa = 3.94 GAASDD334 pKa = 3.84 SLATFALAAIYY345 pKa = 10.58 SFF347 pKa = 4.19
Molecular weight: 36.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.733
IPC2_protein 3.783
IPC_protein 3.821
Toseland 3.579
ProMoST 3.999
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.795
Rodwell 3.643
Grimsley 3.49
Solomon 3.821
Lehninger 3.783
Nozaki 3.935
DTASelect 4.24
Thurlkill 3.643
EMBOSS 3.795
Sillero 3.948
Patrickios 0.655
IPC_peptide 3.808
IPC2_peptide 3.923
IPC2.peptide.svr19 3.832
Protein with the highest isoelectric point:
>tr|A0A495EC27|A0A495EC27_9FLAO Thioredoxin OS=Maribacter vaceletii OX=1206816 GN=CLV91_0510 PE=3 SV=1
MM1 pKa = 7.54 SVRR4 pKa = 11.84 KK5 pKa = 9.68 LKK7 pKa = 10.5 PITPGQRR14 pKa = 11.84 FRR16 pKa = 11.84 VVNGFDD22 pKa = 5.32 AITADD27 pKa = 3.83 KK28 pKa = 10.62 PEE30 pKa = 4.36 KK31 pKa = 10.61 SLLAPLKK38 pKa = 10.66 KK39 pKa = 10.41 SGGRR43 pKa = 11.84 NSQGKK48 pKa = 5.87 MTMRR52 pKa = 11.84 HH53 pKa = 5.73 RR54 pKa = 11.84 GGGHH58 pKa = 5.08 KK59 pKa = 9.74 RR60 pKa = 11.84 RR61 pKa = 11.84 YY62 pKa = 9.28 RR63 pKa = 11.84 VIDD66 pKa = 4.1 FKK68 pKa = 11.12 RR69 pKa = 11.84 DD70 pKa = 3.57 KK71 pKa = 10.71 QDD73 pKa = 2.61 VAATVKK79 pKa = 10.68 SIEE82 pKa = 4.02 YY83 pKa = 10.15 DD84 pKa = 3.43 PNRR87 pKa = 11.84 TAFIALLEE95 pKa = 4.26 YY96 pKa = 10.99 ADD98 pKa = 4.12 GVKK101 pKa = 10.19 KK102 pKa = 10.6 YY103 pKa = 10.48 IIAQNGLQVGQKK115 pKa = 9.74 VSSGQGAAPEE125 pKa = 4.24 VGNALALSEE134 pKa = 4.55 IPLGTVISCIEE145 pKa = 3.9 LRR147 pKa = 11.84 PGQGAVMARR156 pKa = 11.84 SAGTFAQLMAKK167 pKa = 9.93 DD168 pKa = 4.08 GKK170 pKa = 10.42 FVTVKK175 pKa = 9.72 MPSGEE180 pKa = 4.06 TRR182 pKa = 11.84 LILSGCMATIGAISNSDD199 pKa = 3.23 HH200 pKa = 6.21 QLLVSGKK207 pKa = 9.87 AGRR210 pKa = 11.84 TRR212 pKa = 11.84 WLGKK216 pKa = 9.9 RR217 pKa = 11.84 PRR219 pKa = 11.84 TRR221 pKa = 11.84 PVAMNPVDD229 pKa = 3.63 HH230 pKa = 7.13 PMGGGEE236 pKa = 3.89 GRR238 pKa = 11.84 ASGGHH243 pKa = 5.13 PRR245 pKa = 11.84 SRR247 pKa = 11.84 NGIPAKK253 pKa = 10.36 GFRR256 pKa = 11.84 TRR258 pKa = 11.84 SEE260 pKa = 4.05 TKK262 pKa = 9.05 STNKK266 pKa = 10.46 YY267 pKa = 9.26 IIEE270 pKa = 4.03 RR271 pKa = 11.84 RR272 pKa = 11.84 KK273 pKa = 10.1 KK274 pKa = 9.82
Molecular weight: 29.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.308
IPC2_protein 9.853
IPC_protein 10.657
Toseland 11.082
ProMoST 10.847
Dawson 11.125
Bjellqvist 10.847
Wikipedia 11.345
Rodwell 11.403
Grimsley 11.155
Solomon 11.301
Lehninger 11.272
Nozaki 11.052
DTASelect 10.833
Thurlkill 11.052
EMBOSS 11.491
Sillero 11.067
Patrickios 11.111
IPC_peptide 11.316
IPC2_peptide 9.721
IPC2.peptide.svr19 8.636
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3317
0
3317
1184146
30
4889
357.0
40.22
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.251 ± 0.042
0.741 ± 0.015
5.394 ± 0.041
6.682 ± 0.046
5.082 ± 0.032
6.567 ± 0.044
1.718 ± 0.018
8.027 ± 0.042
8.329 ± 0.067
9.111 ± 0.052
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.096 ± 0.023
6.464 ± 0.045
3.418 ± 0.024
3.028 ± 0.021
3.144 ± 0.029
6.527 ± 0.031
6.01 ± 0.057
6.196 ± 0.031
1.134 ± 0.017
4.083 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here